新扶正除疫颗粒抗柯萨奇病毒B3作用评价
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摘要
目的评价新扶正除疫颗粒抗柯萨奇病毒B3(CVB3)感染的效果。方法将60只小鼠随机分为空白对照组、病毒模型组(A组)、新扶正除疫颗粒低、中、高剂量组(B、C、D组)、阳性药物对照组(E组)。给除空白对照组以外的每只小鼠腹腔注射50μL柯萨奇病毒液(10×TCID50)。每天观察小鼠的发病体征及死亡情况,计算生存率,检测病毒滴度,观察心肺组织病理变化。结果给药第3天时D组死亡率仅10%,第7天时仅为20%,显著低于A组、B组和C组,与E组基本相同。检测不同组心、肺中病毒滴度,发现D组显著低于A组、B组和C组(P<0.01)。在比较小鼠脏器组织病理观察方面,D组与E组对肺组织的影响结果相似,病理变化轻微,炎症反应明显弱于A组。结论新扶正除疫颗粒能降低柯萨奇病毒B3感染小鼠的死亡率和病毒滴度,对心肺组织有保护作用。
Objective To evaluate the effect of new Fuzheng Chuyi granule in addition to the plague particles against coxsackie virus B3(CVB3). Methods 60 mice were randomly divided into blank control group, virus model group(group A), and the new Fuzheng Chuyi granule with low, middle and high dose group(B, C, D group) and the positive drug control group(group E). Intraperitoneal injection of 50 ul coxsackie venom 10×TCID50) was injected into the abdominal cavity of every mouse except the blank control group. The morbidity and mortality of mice were observed daily and the survival rate was calculated. To detect the drop of the virus, observe the pathologic changes of the heart and lungs. Results The death rate of group D was only 10% on the third day, and only 20% in 7 days, which was significantly lower than group A, group B and group C, and which was the same as group E. The titer of virus in heart and lung of group D was significantly lower than that of group A and group C(P < 0. 01). In comparison with the pathological observation of organ tissues in mice, the results of group D and E were similar to that of lung tissue, and the pathological changes were slight, and the inflammatory response was significantly weaker than that of group A. Conclusion The new Fuzheng Chuyi granule reduced the mortality and titer of Coxsackievirus B3 infected mice, and had protective effect on heart and lung tissue.
Objective To evaluate the effect of new Fuzheng Chuyi granule in addition to the plague particles against coxsackie virus B3(CVB3). Methods 60 mice were randomly divided into blank control group, virus model group(group A), and the new Fuzheng Chuyi granule with low, middle and high dose group(B, C, D group) and the positive drug control group(group E). Intraperitoneal injection of 50 ul coxsackie venom 10×TCID50) was injected into the abdominal cavity of every mouse except the blank control group. The morbidity and mortality of mice were observed daily and the survival rate was calculated. To detect the drop of the virus, observe the pathologic changes of the heart and lungs. Results The death rate of group D was only 10% on the third day, and only 20% in 7 days, which was significantly lower than group A, group B and group C, and which was the same as group E. The titer of virus in heart and lung of group D was significantly lower than that of group A and group C(P < 0. 01). In comparison with the pathological observation of organ tissues in mice, the results of group D and E were similar to that of lung tissue, and the pathological changes were slight, and the inflammatory response was significantly weaker than that of group A. Conclusion The new Fuzheng Chuyi granule reduced the mortality and titer of Coxsackievirus B3 infected mice, and had protective effect on heart and lung tissue.
引文
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[15]宫晓燕,曲晓波,王泽玉.扶正除疫颗粒对感染流感病毒小鼠的保护作用[J].中药新药与临床药理,2006,17(3):184-185.
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[18]苏鑫,宫晓燕.新扶正除疫颗粒对感染肺炎链球菌小鼠肺组织TLR2,TLR4 m RNA的影响[J].中华中医药杂志,2016,31(8):3302-3304.
[2]郭予洁,伍伟锋.柯萨奇病毒B3与病毒性心脏病发病机制的研究进展[J].医学综述,2016,22(11):2081-2085.
[3]邵惠训.柯萨奇B组病毒与病毒性心肌炎[J].首都公共卫生,2011,5(3):120-124.
[4]WHEELER D S,KOOY N W.A formidable challenge:the diagnosis and treatment of viral myocarditis in children[J].Critical Care Clinics,2003,19(3):365-391.
[5]ESFANDIAREI M,MCMANUS B M.Molecular biology and pathogenesis of viral myocarditis[J].Annual Review of Pathology,2008(3):127-155.
[6]孙非,于起福,孙寒.高山红景天多糖对病毒感染大鼠心肌细胞的抑制作用[J].中国药理学通报,2007,13(6):525.
[7]张勇,刘小玲,刘小雷.红景天多糖体外抗CVB3病毒活性[J].中国医院药学杂志,2009,29(20):1749-1752.
[8]袁琦,侯林,崔清华,等.红景天甲醇冷浸提取物的体外抗病毒谱及抗病毒机制的研究[J].中华中医药学刊,2017,35(7):1-4.
[9]KAMAR N,IZOPET J,TRIPON S,et al.Ribavirin for chronic hepatitis E virus infection in transplant recipients[J].The New England Journal of Medicine,2014,370(12):1111-1120.
[10]YU Yuchen,Jiang Hui.New strategies against drug resistance to herpes simplex virus[J].International Journal of Oral Science,2016,8(1):1-6.
[11]李小青,张国成,许东亮,等.黄芪和大青叶治疗小鼠病毒性心肌炎的对比研究[J].中国当代儿科杂志,2003,5(5):439-442.
[12]刘钊,杨占秋,肖红.中药大青叶有效单体抗柯萨奇病毒作用[J].中南民族大学学报(自然科学版),2009,28(2):41-45.
[13]薛岚.中药虎杖的药理研究进展[J].中国中药杂志,2000,25(11):651.
[14]杨洁,刘萍,武晓玉.5种中药提取物体外抗病毒药效学研究[J].军医进修学院学报,2007,28(5):375-376.
[15]宫晓燕,曲晓波,王泽玉.扶正除疫颗粒对感染流感病毒小鼠的保护作用[J].中药新药与临床药理,2006,17(3):184-185.
[16]岳冬辉,王化磊,宫晓燕,等.扶正除疫颗粒对A型H1N1流感病毒感染小鼠T细胞亚群的影响[J].中华中医药杂志,2014,29(3):704-707.
[17]岳冬辉,高玉伟,宫晓燕,等.扶正除疫颗粒抗甲型H1N1流感病毒感染作用评价[J].中医杂志,2014,55(23):2029.
[18]苏鑫,宫晓燕.新扶正除疫颗粒对感染肺炎链球菌小鼠肺组织TLR2,TLR4 m RNA的影响[J].中华中医药杂志,2016,31(8):3302-3304.