摘要
采用线粒体COⅠ基因序列对我国长江中下游达氏鲌天然种群的遗传变异进行研究。结果表明,达氏鲌不同自然种群的遗传变异程度较低,基因多态性为0.412 3,核苷酸多态性为0.001 1。各群体间特有单倍型较少,多数个体分布于共享单倍型中。分子变异分析(AMOVA)表明,达氏鲌野生群体间具有明显的遗传分化,群体间的变异为33.09%,丹江口种群解释了群体间该遗传变异组成的71.47%,其余的达氏鲌群体间无明显分化,种群间存在一定的基因流动。达氏鲌自然种群的遗传组成可能与不同群体所处的地理位置有关,水系间的相互连通,不同地理种群间存在基因交流,可能减弱了由地理距离产生的种群间的遗传差异。
引文
[1]陈宜瑜. 中国动物志:硬骨鱼纲鲤形目(中卷)[M]. 北京:科学出版社,1998.
[2]陈清华,刘伟,唐富江. 兴凯湖4种野生鲌类营养成分的比较分析[J]. 大连海洋大学学报,2011,26(1):88-92.
[3]王琨,程宝晶,刘斌,等. 不同年龄野生和养殖兴凯湖翘嘴鲌肌肉营养成分分析[J]. 中国水产科学,2012,19(5):906-912.
[4]Skaala O,Hoyheim B,Glover K,et al. Microsatellite analysis in domesticated and wild Atlantic salmon (Salmo salar L.):allelic diversity and identification of individuals[J]. Aquaculture,2004,240(1/2/3/4):131-143.
[5]杨太有,陈宏喜,刘向奇,等. 丹江口水库翘嘴红鲌(Erythroculter ilishaeformis)遗传多样性的RAPD和ISSR分析[J]. 海洋与湖沼,2008,39(3):240-244.
[6]李思发,吕国庆,贝纳切兹 L. 长江中下游鲢鳙草青四大家鱼线粒体DNA多样性分析[J]. 动物学报,1998,44(1):82-93.
[7]杨建远,张小谷,孙丽群. 江西鄱阳湖达氏鲌生长特征研究[J]. 江西农业大学学报,2009,31(2):336-340.
[8]彭居俐,王绪祯,王丁,等. 基于线粒体COⅠ基因序列的DNA条形码在鲤科鲌属鱼类物种鉴定中的应用[J]. 水生生物学报,2009,3(2):271-276.
[9]王丹,程庆武,杨镇宇,等. 三峡库区鲌属鱼类线粒体COⅠ基因遗传多样性的初步分析[J]. 水生生物学报,2015,39(5):1054-1058.
[10]萨姆布鲁克 J,拉塞尔D W. 分子克隆实验指南[M]. 黄培堂,译. 3版. 北京:科学出版社,2002:463.
[11]Ward R D,Zemlak T S,Innes B H,et al. DNA barcoding Australia's fish species[J]. Philosophical Transactions of the Royal Society of London. Series B,Biological Sciences,2005,360(1462):1847-1857.
[12]Thompson J D,Gibson T J,Plewniak F,et al. The clustal X Windows interface:flexible strategies for multiple sequences alignment aided by quality analysis tools[J]. Nucleic Acids Research,1997,25(24):4876-4882.
[13]Gouy M,Guindon S,Gascuel O. SeaView version 4:a multiplatform graphical user interface for sequence alignment and phylogenetic tree building[J]. Molecular Biology and Evolution,2010,27(2):221-224.
[14]Tamura K,Stecher G,Peterson D,et al. MEGA6:molecular evolutionary genetics analysis version 6.0[J]. Molecular Biology and Evolution,2013,30(12):2725-2729.
[15]Excoffier L,Lischer H E. Arlequin suite ver 3.5:a new series of programs to perform population genetics analyses under Linux and Windows[J]. Molecular Ecology Resources,2010,10(3):564-567.
[16]He S P,Liu H Z,Chen Y Y,et al. Molecular phylogenetic relationships of Eastern Asian Cyprinidae (Pisces:Cypriniformes) inferred from cytochrome b sequences[J]. Science in China Series C-Life Sciences,2004,47(2):130-138.
[17]Hebert P D,Cywinska A,Ball S L,et al. Biological identifications through DNA barcodes[J]. Proceedings. Biological Sciences / the Royal Society,2003,270(1512):313-321.
[18]Schlei O L,Crete-Lafreniere A,Whiteley A R,et al. DNA barcoding of eight North American coregonine species[J]. Molecular Ecology Resources,2008,8(6):1212-1218.
[19]唐伟,朱治任,汪财生,等. 基于COⅠ基因的DNA条形码在鳖科动物鉴定上的应用[J]. 江苏农业科学,2017,45(6):30-36.
[20]Hebert P D,Stoeckle M Y,Zemlak T S,et al. Identification of birds through DNA barcodes[J]. PLOS Biology,2004,2(10):1657-1663.
[21]施立明. 遗传多样性及其保存[J]. 生物科学信息,1990,2(4):158-164.
[22]王伟,陈立侨,禹娜,等. 应用COⅡ基因部分序列分析翘嘴鲌群体的遗传多样性[J]. 大连水产学院学报,2008,23(5):403-408.
[23]Grant W S,Bowen B W. Shallow population histories in deep evolutionary lineages of marine fishes:insights from sardines and anchovies and lessons for conservation[J]. Journal of Heredity,1998,89(5):415-426.
[24]He D K,Kang Z J,Tao J,et al. Hydrologic connectivity driven natural stream fish assemblages in mountain streams in the Yangtze River basin:implications for stream fish conservation in monsoonal East Asia[J]. Hydrobiologia,2017,785(1):185-206.
[25]李修峰,黄道明,谢文星. 汉江中游产漂流性卵鱼类产卵场的现状[J]. 大连海洋大学学报,2006,21(2):105-111.
[26]谢文星,黄道明,谢山,等. 丹江口水利枢纽兴建后汉江中下游四大家鱼等早期资源及其演变[J]. 水生态学杂志,2009,2(2):44-49.孔杰,周洲,刘伟,等. 达氏鳇亲鱼群体mtDNA Cyt b基因和D-loop区的序列特征及遗传多样性分析[J]. 江苏农业科学,2019,47(3):40-42.