烟草野火病菌的基因组学分析及其致病性分化研究进展
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摘要
烟草野火病是世界普遍发生的一种细菌性病害。本文从野火病菌基因组学和致病性两方面归纳了烟草野火病的研究现状,总结了5个已完成测序的丁香假单胞菌属的基因组特征、致病岛、T3SS及其毒力研究、基因水平转移与病原菌寄主适应性进化,分析了野火病菌株的致病机理和生理小种,并对烟草野火病的深入研究方向进行了展望。
Tobacco wildfire disease is a common bacterial disease in the world. To explore current progress on tobacco wildfire disease research, this paper summarized present research status of wildfire disease in tobacco based on Pseudomonas syringae pave. tabaci genomics and pathogenicity. Genomic features, pathogenicity island, type Ⅲ secretion system and virulence, horizontal gene transfer and adaptive evolution between pathogen and host of 5 Pseudomonas syringae strains were investigated. Pathogenic mechanism, physioloigical race of tobacco wildfire strain were analyzed. Suggestions were put forward for further research on tobacco wildfire disease.
Tobacco wildfire disease is a common bacterial disease in the world. To explore current progress on tobacco wildfire disease research, this paper summarized present research status of wildfire disease in tobacco based on Pseudomonas syringae pave. tabaci genomics and pathogenicity. Genomic features, pathogenicity island, type Ⅲ secretion system and virulence, horizontal gene transfer and adaptive evolution between pathogen and host of 5 Pseudomonas syringae strains were investigated. Pathogenic mechanism, physioloigical race of tobacco wildfire strain were analyzed. Suggestions were put forward for further research on tobacco wildfire disease.
引文
[1]Lucas G B.Diseases of Tobacco[M].(Third edition)Raleigh North Carolina.U.S.A.1975.
[2]谈文.烟草病理学教程[M].北京:中国科学技术出版社,1995,149-153.TAN Wen.Tobacco pathology tutorial[M].Beijing:Science and Technology of China Press,1995,149-153.
[3]Young J M.Taxonomy of Pseudomonas syringae[J].Journal of Plant Pathology,2010,92(1):S1.5-S1.14.
[4]Joardar V,Lindeberg M,Jackson R W,et al.Whole-Genome Sequence Analysis of Pseudomonas syringae pv.phaseolicola1448A Reveals Divergence among Pathovars in Genes Involved in Virulence and Transposition[J].Journal of Bacteriology,2005,187(18):6488-98.
[5]Feil H,Feil W S,Chain P,et al.Comparison of the Complete Genome Sequences of Pseudomonas syringae pv.syringae B728a and pv.tomato DC3000[J].Proceedings of the National Academy of Sciences of the United States of America,2005,102(31):11064-9.
[6]Buell C R,Joardar V,Lindeberg M,et al.The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv.tomato DC3000[J].Proceedings of the National Academy of Sciences of the United States of America,2003,100(18):10181.
[7]Studholme D J,Ibanez S G,Maclean D,et al.A draft genome sequence and functional screen reveals the repertoire of type III secreted proteins of Pseudomonas syringae pathovar tabaci11528[J].Bmc Genomics,2009,10(1):569.
[8]Wang T,Yang Y,Zhao T.Genome Sequence of a Pseudomonas syringae pv.tabaci Strain,yuexi-1,Causing Wildfire Disease in Tobacco[J].Genome Announcements,2015,3(2).
[9]Gutiérrez-Barranquero J A,Cazorla F M,Vicente A D,et al.Complete sequence and comparative genomic analysis of eight native Pseudomonas syringae,plasmids belonging to the pPT23Afamily[J].Bmc Genomics,2017,18(1):365.
[10]Everett K R,Taylor R K,Romberg M K,et al.First report of Pseudomonas syringae pv.actinidiae causing kiwifruit bacterial canker in New Zealand[J].Australasian Plant Disease Notes,2011,6(1):67-71.
[11]Bastas K K,Karakaya A.First report of bacterial canker of kiwifruit caused by Pseudomonas syringae pv.actinidiae in Turkey[J].Plant Disease,2012,96(3):452-452.
[12]Baltrus D A,Nishimura M T,Romanchuk A,et al.Dynamic Evolution of Pathogenicity Revealed by Sequencing and Comparative Genomics of 19 Pseudomonas syringae Isolates[J].Plos Pathogens,2011,7(7):e1002132.
[13]李江利.丁香假单胞菌番茄致病变种和烟草致病变种egfp标记突变体的构建[D].河南农业大学,2011.L I J i a n g l i.C o n s t r u c t i o n o f e g f p M a f k e d-M u t a n t s f r o m Pseudomonas syringae pv.tomato and pv.tabaci[D].Henan Agricultural University,2011.
[14]Lindeberg M,Myers C R,Collmer A,et al.Roadmap to new virulence determinants in Pseudomonas syringae:insights from comparative genomics and genome organization[J].Molecular Plant-Microbe Interactions,2008,21(6):685-700.
[15]Studholme D J,Ibanez S G,Maclean D,et al.A draft genome sequence and functional screen reveals the repertoire of type IIIsecreted proteins of Pseudomonas syringae,pathovar tabaci,11528[J].Bmc Genomics,2009,10(1):395.
[16]Studholme D J,Ibanez S G,Maclean D,et al.A draft genome sequence and functional screen reveals the repertoire of type IIIsecreted proteins of Pseudomonas syringae,pathovar tabaci,11528[J].Bmc Genomics,2009,10(1):395.
[17]Yao J,Allen C.Chemotaxis is required for virulence and competitive fitness of the bacterial wilt pathogen Ralstonia solanacearum.[J].J Bacterio,2006,188(10):3697-3708.
[18]Gomez-Gomez L,Boller T.Flagellin perception:a paradigm for innate immunity[Review][J].Trends in Plant Science,2002,7(6):251.
[19]Tseng T T,Tyler B M,Setubal J C.Protein secretion systems in bacterial-host associations,and their description in the Gene Ontology.[J].Bmc Microbiology,2009,9(Suppl 1):S2.
[20]Yang H J,Lee J S,Cha J Y,et al.Negative regulation of pathogenesis in Pseudomonas syringae pv.tabaci 11528 by ATP-dependent Lon protease.[J].Molecules&Cells,2011,32(4):317-323.
[21]Collmer A,Lindeberg M,Petnickiocwieja T,et al.Genomic mining type III secretion system eff ectors in Pseudomonas syringae yields new picks for all TTSS prospectors.[J].Trends in Microbiology,2002,10(10):462.
[22]Hutcheson S W,Bretz J,Sussan T,et al.Enhancer-binding proteins HrpR and HrpS interact to regulate hrp-encoded type IIIprotein secretion in Pseudomonas syringae strains.[J].Journal of Bacteriology,2001,183(19):5589.
[23]王育鹏,刘登义,李征,等.自然植物种群中病原菌与寄主植物相互作用的遗传学[J].生态学杂志,2005,24(2):190-194.WANG Yupeng,LIU Deng yi,LI Zheng,et al.Genetics aspects of interactions between pathogenic fungi and host plants in natural plant population[J].Chinese Journal of Ecology,2005,24(2):190-194.
[24]牟志霞.致病细菌三型分泌系统(T3SS)高度保守的ATPase结构与功能研究[D].北京协和医学院23中国医学科学院,北京协和医学院,中国医学科学院,清华大学医学部,2015.MOU Zhixia.Structural and Functional Study of the Highly Conserved ATPase from Type III Secretion System of Bacterial Pathogens[D].Chinese Academy of Medical Sciences,Peking Union Medical College,2015.
[25]Lucas GB.Wildfi re and angular leaf spot.Diseases of tobacco,3rd ed.Biological Consulting Associates,Raleigh,NC,1975:379-409
[26]Hirano S S,Upper C D.Bacteria in the leaf ecosystem with emphasis on Pseudomonas syringae-a pathogen,ice nucleus,and epiphyte.[J].Microbiology&Molecular Biology Reviews,2000,64(3):624-653.
[27]王振国,丁伟.烟草野火病发生与防治的研究进展[J].中国烟草学报,2012,18(2):101-106.WANG Zhenguo,DING Wei.Research progeress in tocacco wildfi re disease’s occurrence and control[J].ACTA TABACARIASINICA,2012,18(2):101-106.
[28]Deall M W,Cole J S.A comparative study of the pathogenicity and epidemiology of strains of Pseudomonas syringae pv.tabaci that cause wildfire and angular leaf spot diseases of tobacco in Zimbabwe[J].Plant Pathology,1986,35(1):74-81.
[29]刘秋,吴元华,于基成.烟草野火病的研究进展[J].沈阳农业大学学报,1999(3):354-360.LIU Qiu,WU Yuanhua,YU Jicheng.Research prospect of tobacco wildfire disease[J].Journal of Shenyang Agricultural University,1999(3):354-360.
[30]高必达.植物抗病基因工程研究进展[J].湖南农学院学报,1994,20(6):587-596.GAO Bida.Advances in the research of plant disease resistance genetic engineering[J].Journal of Hunan Agricultural College,1994,20(6):587-596.
[31]徐玲,张世珖.云南省烟草野火病菌毒素的致病性评价[J].云南农业大学学报,2007,22(5):650-653.XU Ling,ZHANG Shiguang.Research prospect of the tabtoxin’s pathogenicity[J].Journal of Yunnan Agricultural University,2007,22(5):50-653.
[32]Arrebola E,Cazorla F M,Pérezgarcía A,et al.Genes Involved in the Production of Antimetabolite Toxins by Pseudomonas syringae Pathovars[J].Genes,2011,2(3):640.
[33]Ito M,Yamamoto Y,Kim C S,et al.Heat shock protein 70 is required for tabtoxinine-β-lactam-induced cell death in Nicotiana benthamiana[J].Journal of Plant Physiology,2014,171(2):173-8.
[34]Ito M,Takahashi H,Sawasaki T,et al.Novel type of adenylyl cyclase participates in tabtoxinine-β-lactam-induced cell death and occurrence of wildfi re disease in Nicotiana benthamiana[J].Plant Signaling&Behavior,2014,9(1).
[35]Taylor P A,Schnoes H K,Durbin R D.Characterization of chlorosis-inducing toxins from a plant pathogenic Pseudomonas Sp[J].Biochimica et Biophysica Acta(BBA)-General Subjects,1972,86(1):107-117.
[36]Popham P L,Pike S M,Novacky A,et al.Water Relation Alterations Observed during Hypersensitive Reaction Induced by Bacteria[J].Plant Physiology,1993,103(4):1243-1247.
[37]Baker C J,O'Neill N R,Keppler L D,et al.Early responses during plant-bacteria interactions in tobacco cell suspensions[J].Phytopathology,1991,81(12):1504-1507.
[38]Lee S,Yang D S,Uppalapati S R,et al.Suppression of plant defense responses by extracellular metabolites from Pseudomonas syringae pv.tabaci in Nicotiana benthamiana[J].Bmc Plant Biology,2013,13(1):65.
[39]Franco M C,Sta C,Montrazzi F C,et al.RAPD analysis common bean(Phaseolus vulgaris L.)cultivars and evaluation of common bacterial blight(CBB)and wild fire(WF)resistance[J].Bean Improvement Cooperative,1998.
[40]Knoche K K,Clayton M K,Fulton R W.Comparison of resistance in tobacco to Pseudomonas syringae pv.tabaci races0 and 1 by infectivity titrations and bacterial multiplications.[J].Phytopathology,1987,77(9):1364-1368.
[41]Fulton R W.Tobacco blackfire disease in Wisconsin[J].Plant Disease,1980,64(1):100-100.
[42]Cole D L and Mapuranga N.Reaction of indicator tobacco cultivars to races of Pseudomonas syringae pv.tabaci Tox+[J].Contributions to Tobacco Research.2001,19(7):353-359.
[43]彭润,张世珖,熊立,等.云南省烟草野火病菌生理小种分化的研究[J].植物病理学报,2005,35(1):1-5.PENG Run,ZHANG Shiguang,XIONG Li,et al.Physiological race diff erentiation of Pseudomonas syringae pv.tabaci in Yunnan province[J].Acta Phytopathologica Sinica,2005,35(1):1-5.
[44]陈赟娟,刘雅婷,李永忠,等.云南烟草野火病菌菌系分化研究[J].云南农业大学学报,2007,22(4):491-494.CHEN Yunjuan,LIU Yating,LI Yongzhong,et al.Study on strains dirrerentiation of Pseudomonas syringae pv.tabaci in Yunnan[J].Journal of Yunnan Agricultural University,2007,22(4):491-494.
[45]孙剑萍,孙宏伟,虞艳芳.黑龙江省烟草野火病菌生理小种鉴别寄主的筛选[J].烟草科技,2011(10):75-77.SUN Jianping,SUN Hongwei,YUN Yanfang.Differential Host Screen for Physiologic Races of Pseudomonas syringae pv.tabaci in Heilongjiang[J].Tobacco Science and Technology,2011(10):75-77.
[46]隋原.吉林和黑龙江两省烟草野火病菌遗传多样性研究[D].吉林农业大学,2011.SUI Yuan.Genetic Diversity of Pseudomonas Syringae Pv.Tabaci from Jilin and Heilongjiang Provinces of China[D].Jilin Agricultural University,2011.
[2]谈文.烟草病理学教程[M].北京:中国科学技术出版社,1995,149-153.TAN Wen.Tobacco pathology tutorial[M].Beijing:Science and Technology of China Press,1995,149-153.
[3]Young J M.Taxonomy of Pseudomonas syringae[J].Journal of Plant Pathology,2010,92(1):S1.5-S1.14.
[4]Joardar V,Lindeberg M,Jackson R W,et al.Whole-Genome Sequence Analysis of Pseudomonas syringae pv.phaseolicola1448A Reveals Divergence among Pathovars in Genes Involved in Virulence and Transposition[J].Journal of Bacteriology,2005,187(18):6488-98.
[5]Feil H,Feil W S,Chain P,et al.Comparison of the Complete Genome Sequences of Pseudomonas syringae pv.syringae B728a and pv.tomato DC3000[J].Proceedings of the National Academy of Sciences of the United States of America,2005,102(31):11064-9.
[6]Buell C R,Joardar V,Lindeberg M,et al.The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv.tomato DC3000[J].Proceedings of the National Academy of Sciences of the United States of America,2003,100(18):10181.
[7]Studholme D J,Ibanez S G,Maclean D,et al.A draft genome sequence and functional screen reveals the repertoire of type III secreted proteins of Pseudomonas syringae pathovar tabaci11528[J].Bmc Genomics,2009,10(1):569.
[8]Wang T,Yang Y,Zhao T.Genome Sequence of a Pseudomonas syringae pv.tabaci Strain,yuexi-1,Causing Wildfire Disease in Tobacco[J].Genome Announcements,2015,3(2).
[9]Gutiérrez-Barranquero J A,Cazorla F M,Vicente A D,et al.Complete sequence and comparative genomic analysis of eight native Pseudomonas syringae,plasmids belonging to the pPT23Afamily[J].Bmc Genomics,2017,18(1):365.
[10]Everett K R,Taylor R K,Romberg M K,et al.First report of Pseudomonas syringae pv.actinidiae causing kiwifruit bacterial canker in New Zealand[J].Australasian Plant Disease Notes,2011,6(1):67-71.
[11]Bastas K K,Karakaya A.First report of bacterial canker of kiwifruit caused by Pseudomonas syringae pv.actinidiae in Turkey[J].Plant Disease,2012,96(3):452-452.
[12]Baltrus D A,Nishimura M T,Romanchuk A,et al.Dynamic Evolution of Pathogenicity Revealed by Sequencing and Comparative Genomics of 19 Pseudomonas syringae Isolates[J].Plos Pathogens,2011,7(7):e1002132.
[13]李江利.丁香假单胞菌番茄致病变种和烟草致病变种egfp标记突变体的构建[D].河南农业大学,2011.L I J i a n g l i.C o n s t r u c t i o n o f e g f p M a f k e d-M u t a n t s f r o m Pseudomonas syringae pv.tomato and pv.tabaci[D].Henan Agricultural University,2011.
[14]Lindeberg M,Myers C R,Collmer A,et al.Roadmap to new virulence determinants in Pseudomonas syringae:insights from comparative genomics and genome organization[J].Molecular Plant-Microbe Interactions,2008,21(6):685-700.
[15]Studholme D J,Ibanez S G,Maclean D,et al.A draft genome sequence and functional screen reveals the repertoire of type IIIsecreted proteins of Pseudomonas syringae,pathovar tabaci,11528[J].Bmc Genomics,2009,10(1):395.
[16]Studholme D J,Ibanez S G,Maclean D,et al.A draft genome sequence and functional screen reveals the repertoire of type IIIsecreted proteins of Pseudomonas syringae,pathovar tabaci,11528[J].Bmc Genomics,2009,10(1):395.
[17]Yao J,Allen C.Chemotaxis is required for virulence and competitive fitness of the bacterial wilt pathogen Ralstonia solanacearum.[J].J Bacterio,2006,188(10):3697-3708.
[18]Gomez-Gomez L,Boller T.Flagellin perception:a paradigm for innate immunity[Review][J].Trends in Plant Science,2002,7(6):251.
[19]Tseng T T,Tyler B M,Setubal J C.Protein secretion systems in bacterial-host associations,and their description in the Gene Ontology.[J].Bmc Microbiology,2009,9(Suppl 1):S2.
[20]Yang H J,Lee J S,Cha J Y,et al.Negative regulation of pathogenesis in Pseudomonas syringae pv.tabaci 11528 by ATP-dependent Lon protease.[J].Molecules&Cells,2011,32(4):317-323.
[21]Collmer A,Lindeberg M,Petnickiocwieja T,et al.Genomic mining type III secretion system eff ectors in Pseudomonas syringae yields new picks for all TTSS prospectors.[J].Trends in Microbiology,2002,10(10):462.
[22]Hutcheson S W,Bretz J,Sussan T,et al.Enhancer-binding proteins HrpR and HrpS interact to regulate hrp-encoded type IIIprotein secretion in Pseudomonas syringae strains.[J].Journal of Bacteriology,2001,183(19):5589.
[23]王育鹏,刘登义,李征,等.自然植物种群中病原菌与寄主植物相互作用的遗传学[J].生态学杂志,2005,24(2):190-194.WANG Yupeng,LIU Deng yi,LI Zheng,et al.Genetics aspects of interactions between pathogenic fungi and host plants in natural plant population[J].Chinese Journal of Ecology,2005,24(2):190-194.
[24]牟志霞.致病细菌三型分泌系统(T3SS)高度保守的ATPase结构与功能研究[D].北京协和医学院23中国医学科学院,北京协和医学院,中国医学科学院,清华大学医学部,2015.MOU Zhixia.Structural and Functional Study of the Highly Conserved ATPase from Type III Secretion System of Bacterial Pathogens[D].Chinese Academy of Medical Sciences,Peking Union Medical College,2015.
[25]Lucas GB.Wildfi re and angular leaf spot.Diseases of tobacco,3rd ed.Biological Consulting Associates,Raleigh,NC,1975:379-409
[26]Hirano S S,Upper C D.Bacteria in the leaf ecosystem with emphasis on Pseudomonas syringae-a pathogen,ice nucleus,and epiphyte.[J].Microbiology&Molecular Biology Reviews,2000,64(3):624-653.
[27]王振国,丁伟.烟草野火病发生与防治的研究进展[J].中国烟草学报,2012,18(2):101-106.WANG Zhenguo,DING Wei.Research progeress in tocacco wildfi re disease’s occurrence and control[J].ACTA TABACARIASINICA,2012,18(2):101-106.
[28]Deall M W,Cole J S.A comparative study of the pathogenicity and epidemiology of strains of Pseudomonas syringae pv.tabaci that cause wildfire and angular leaf spot diseases of tobacco in Zimbabwe[J].Plant Pathology,1986,35(1):74-81.
[29]刘秋,吴元华,于基成.烟草野火病的研究进展[J].沈阳农业大学学报,1999(3):354-360.LIU Qiu,WU Yuanhua,YU Jicheng.Research prospect of tobacco wildfire disease[J].Journal of Shenyang Agricultural University,1999(3):354-360.
[30]高必达.植物抗病基因工程研究进展[J].湖南农学院学报,1994,20(6):587-596.GAO Bida.Advances in the research of plant disease resistance genetic engineering[J].Journal of Hunan Agricultural College,1994,20(6):587-596.
[31]徐玲,张世珖.云南省烟草野火病菌毒素的致病性评价[J].云南农业大学学报,2007,22(5):650-653.XU Ling,ZHANG Shiguang.Research prospect of the tabtoxin’s pathogenicity[J].Journal of Yunnan Agricultural University,2007,22(5):50-653.
[32]Arrebola E,Cazorla F M,Pérezgarcía A,et al.Genes Involved in the Production of Antimetabolite Toxins by Pseudomonas syringae Pathovars[J].Genes,2011,2(3):640.
[33]Ito M,Yamamoto Y,Kim C S,et al.Heat shock protein 70 is required for tabtoxinine-β-lactam-induced cell death in Nicotiana benthamiana[J].Journal of Plant Physiology,2014,171(2):173-8.
[34]Ito M,Takahashi H,Sawasaki T,et al.Novel type of adenylyl cyclase participates in tabtoxinine-β-lactam-induced cell death and occurrence of wildfi re disease in Nicotiana benthamiana[J].Plant Signaling&Behavior,2014,9(1).
[35]Taylor P A,Schnoes H K,Durbin R D.Characterization of chlorosis-inducing toxins from a plant pathogenic Pseudomonas Sp[J].Biochimica et Biophysica Acta(BBA)-General Subjects,1972,86(1):107-117.
[36]Popham P L,Pike S M,Novacky A,et al.Water Relation Alterations Observed during Hypersensitive Reaction Induced by Bacteria[J].Plant Physiology,1993,103(4):1243-1247.
[37]Baker C J,O'Neill N R,Keppler L D,et al.Early responses during plant-bacteria interactions in tobacco cell suspensions[J].Phytopathology,1991,81(12):1504-1507.
[38]Lee S,Yang D S,Uppalapati S R,et al.Suppression of plant defense responses by extracellular metabolites from Pseudomonas syringae pv.tabaci in Nicotiana benthamiana[J].Bmc Plant Biology,2013,13(1):65.
[39]Franco M C,Sta C,Montrazzi F C,et al.RAPD analysis common bean(Phaseolus vulgaris L.)cultivars and evaluation of common bacterial blight(CBB)and wild fire(WF)resistance[J].Bean Improvement Cooperative,1998.
[40]Knoche K K,Clayton M K,Fulton R W.Comparison of resistance in tobacco to Pseudomonas syringae pv.tabaci races0 and 1 by infectivity titrations and bacterial multiplications.[J].Phytopathology,1987,77(9):1364-1368.
[41]Fulton R W.Tobacco blackfire disease in Wisconsin[J].Plant Disease,1980,64(1):100-100.
[42]Cole D L and Mapuranga N.Reaction of indicator tobacco cultivars to races of Pseudomonas syringae pv.tabaci Tox+[J].Contributions to Tobacco Research.2001,19(7):353-359.
[43]彭润,张世珖,熊立,等.云南省烟草野火病菌生理小种分化的研究[J].植物病理学报,2005,35(1):1-5.PENG Run,ZHANG Shiguang,XIONG Li,et al.Physiological race diff erentiation of Pseudomonas syringae pv.tabaci in Yunnan province[J].Acta Phytopathologica Sinica,2005,35(1):1-5.
[44]陈赟娟,刘雅婷,李永忠,等.云南烟草野火病菌菌系分化研究[J].云南农业大学学报,2007,22(4):491-494.CHEN Yunjuan,LIU Yating,LI Yongzhong,et al.Study on strains dirrerentiation of Pseudomonas syringae pv.tabaci in Yunnan[J].Journal of Yunnan Agricultural University,2007,22(4):491-494.
[45]孙剑萍,孙宏伟,虞艳芳.黑龙江省烟草野火病菌生理小种鉴别寄主的筛选[J].烟草科技,2011(10):75-77.SUN Jianping,SUN Hongwei,YUN Yanfang.Differential Host Screen for Physiologic Races of Pseudomonas syringae pv.tabaci in Heilongjiang[J].Tobacco Science and Technology,2011(10):75-77.
[46]隋原.吉林和黑龙江两省烟草野火病菌遗传多样性研究[D].吉林农业大学,2011.SUI Yuan.Genetic Diversity of Pseudomonas Syringae Pv.Tabaci from Jilin and Heilongjiang Provinces of China[D].Jilin Agricultural University,2011.