野生/栽培羊肚菌及毛头鬼伞的红外光谱三级鉴别
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摘要
为野生/栽培羊肚菌及毛头鬼伞的开发利用和深入研究提供参考,采用傅里叶变换红外光谱、二阶导数红外光谱和二维相关红外光谱,对野生/栽培羊肚菌(Morchella esculenta)及毛头鬼伞(Coprinus comatus)进行鉴别。结果表明:野生/栽培羊肚菌及毛头鬼伞的原始光谱的整体特征基本相同。野生/栽培羊肚菌在1 750~850cm-1区域时和毛头鬼伞在1 700~900cm-1区域时的二阶导数红外光谱均出现差异。野生/栽培羊肚菌在1 694~1 270cm-1、1 240~872cm-1和480~400cm-1区域时的自动峰数目均有差异;野生/栽培毛头鬼伞在1 680~1 380cm-1与1 210~900cm-1区域时的自动峰数目有差异;野生/栽培间的自动峰和交叉峰的强度有差异。红外光谱三级鉴别法能简单、快速、无损地区分野生/栽培羊肚菌及毛头鬼伞。
The wild/cultivation Morchellaesculenta and Coprinus comatus is identified by Fourier transform infrared spectra(FT-IR),second derivative near-infrared spectra(SD-IR)and two-dimensional correlation infrared spectra(2 D-IR)to provide a reference for development and intensive study of wild/cultivation M.esculenta and C.comatus.Result:The overall feature of original spectrum of wild/cultivation M.esculenta and C.comatus is the same basically.There are differences in SD-IR spectra within 1750~850 cm-1 between wild and cultivation M.esculentaand within 1 700~900 cm-1 between wild and cultivation C.comatus.There is a difference in number of auto peak within 1 694~1 270 cm-1,1 240~872 cm-1 and 480~400 cm-1 between wild and cultivation M.esculenta.There is a difference in number of auto peak within 1 680~1 380 cm-1 and 1 210~900 cm-1 between wild and cultivation C.comatus.There are differences in intensity of auto peak and cross peak between wild and cultivation M.esculentaand between wild and cultivation C.comatus.The three-step infrared spectroscopy method with the simplicity,rapid and undamaged advantage can be used in identification of wild/cultivation M.esculentaand C.comatus.
The wild/cultivation Morchellaesculenta and Coprinus comatus is identified by Fourier transform infrared spectra(FT-IR),second derivative near-infrared spectra(SD-IR)and two-dimensional correlation infrared spectra(2 D-IR)to provide a reference for development and intensive study of wild/cultivation M.esculenta and C.comatus.Result:The overall feature of original spectrum of wild/cultivation M.esculenta and C.comatus is the same basically.There are differences in SD-IR spectra within 1750~850 cm-1 between wild and cultivation M.esculentaand within 1 700~900 cm-1 between wild and cultivation C.comatus.There is a difference in number of auto peak within 1 694~1 270 cm-1,1 240~872 cm-1 and 480~400 cm-1 between wild and cultivation M.esculenta.There is a difference in number of auto peak within 1 680~1 380 cm-1 and 1 210~900 cm-1 between wild and cultivation C.comatus.There are differences in intensity of auto peak and cross peak between wild and cultivation M.esculentaand between wild and cultivation C.comatus.The three-step infrared spectroscopy method with the simplicity,rapid and undamaged advantage can be used in identification of wild/cultivation M.esculentaand C.comatus.
引文
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[20]马殿旭,刘刚,欧全宏,等.七种牛肝菌的红外光谱鉴别[J].光谱学与光谱分析,2016,36(8):2479-2486.
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[3]HELENO S A,STOJKOVID,BARROS L,et al.A comparative study of chemical composition,antioxidant and antimicrobial properties of Morchella esculenta(L.)Pers.from Portugal and Serbia[J].Food Research International,2013,51(1):236-243.
[4]DING Z Y,LU Y J,LU Z X,et al.Hypoglycaemic effect of comatin,an antidiabetic substance separated fromCoprinus comatus broth,on alloxan-induced-diabetic rats[J].Food Chemistry,2010,121(1):39-43.
[5]LIU Y F,ZHAO Y,YANG Y,et al.Structural characteristics and hypoglycemic activity of polysaccharides from Coprinus comatus[J].Bioactive Carbohydrates and Dietary Fibre,2013,2(2):164-169.
[6]中国食用菌协会.2015年中国食用菌总产量及细分市场分析(图)[EB/OL].(2015-09-01)[2017-12-03].http://www.chyxx.com/industry/201509/341282.html.
[7]冮洁,麦海美,解彬,等.羊肚菌菌丝体富硒条件优化及其硒多糖抗氧化活性研究[J].食品与发酵工业,2016,42(9):120-125.
[8]王震,王春弘,蔡英丽,等.羊肚菌人工栽培技术[J].中国食用菌,2016,35(4):87-91.
[9]KESU.Life History and Developmental Processes in the Basidiomycete Coprinus cinereus[J].Microbiology and Molecular Biology Reviews,2000,64(2):316-353.
[10]STOJKOVID,REIS F S,BARROS L,et al.Nutrients and non-nutrients composition and bioactivity of wild and cultivated Coprinus comatus(O.F.Mull.)Pers[J].Food and Chemical Toxicology,2013,59:289-296.
[11]韩文清,杨辉,于得才,等.不同覆土基质特性及其对毛头鬼伞出菇产量的影响[J].食用菌学报,2014,21(4):35-38.
[12]DU X H,ZHAO Q,O’DONNELL K,et al.Multigene molecular phylogenetics reveals true morels(Morchella)are especially species-rich in China[J].Fungal Genetics and Biology,2012,49(6):455-469.
[13]KELLNER H,RENKER C,BUSCOT F.Species diversity within the Morchella esculenta group(Ascomycota:Morchellaceae)in Germany and France[J].Organisms,Diversity&Evolution,2005,5(2):101-107.
[14]孙素琴,周群,陈建波.中药红外光谱分析与鉴定[M].北京:化学工业出版社,2010.
[15]CHOONG Y K,XU C H,LAN J,et al.Identification of geographical origin of Lignosus samples using Fourier transform infrared and two-dimensional infrared correlation spectroscopy[J].Journal of Molecular Structure,2014,1069:188-195.
[16]马殿旭,刘刚,欧全宏,等.乳菇和红菇的红外光谱及其二维相关红外光谱的分析与鉴别[J].光谱学与光谱分析,2016,36(7):2104-2110.
[17]周继国,刘刚,刘剑虹,等.毛头鬼伞的红外光谱研究[J].光谱学与光谱分析,2008,28(2):321-323.
[18]CHOONG Y K,SUN S Q,ZHOU Q,et al.Determination of storage stability of the crude extracts of Ganoderma lucidum using FTIR and 2D-IR spectroscopy[J].Vibrational Spectroscopy,2011,57(1):87-96.
[19]ZHU Y,TAN T L.Penalized discriminant analysis for the detection of wild-grown and cultivated Ganoderma lucidumusing Fourier transform infrared spectroscopy[J].Spectrochim.Acta A,2016,159:68-77.
[20]马殿旭,刘刚,欧全宏,等.七种牛肝菌的红外光谱鉴别[J].光谱学与光谱分析,2016,36(8):2479-2486.