鸡腿菇子实体多糖的体内、体外抗氧化活性
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摘要
以VC为对照,研究鸡腿菇多糖的体外、体内抗氧化活性。结果表明:鸡腿菇多糖对·OH和NO·自由基有较好的清除效果,并且经去蛋白处理的DPPS的清除能力强于未经去蛋白处理的粗多糖CPS,当质量浓度为8mg/mL时,二者对·OH自由基的清除率分别达到62.1%和44%,对NO·的清除率分别达到70.1%和63.1%;另外,该多糖对DPPH的清除率也很高,清除率分别达到62.55%和48.4%;鸡腿菇多糖对O2-·虽也具有一定的清除能力,但效果不明显,当质量浓度为8 mg/mL时,二者的清除率分别为21.2%和29.2%。小鼠实验表明,饲养30 d后,相比于模型组,DPPS组小鼠的心、肝、肾、血中GSH-Px、SOD、CAT及T-AOC的活性显著升高,而MDA含量显著降低,体现出较好的抗氧化作用。
The antioxidant activity in vitro and vivo of polysaccharide from Coprinus comatus were tested and compared with those of VC. The results showed that the polysaccharides from Coprinus comatus had good scavenging effect on ·OH and NO·, moreover, the scavenging rate of DPPS(deproteinated) were higher than CPS(non-deproteinated,crude). The ·OH and NO· radical scavenging rate could reach 62.1%, 70.1% for DPPS and 44% and 63.1% for CPS respectively when the concentration of polysaccharide was 8 mg/mL. The good scavenging effect of DPPS and CPS on·DPPH was also proved with 62.55% and 48.4% respectively. Also, the polysaccharide from Coprinus comatus had a certain scavenging effect on O2-·. Compared with model group, the GSH-Px, SOD, CAT and T-AOC activities of DPPS treatment group were significantly raised, and the MDA content was remarkably reduced. All of this conceived the good antioxidant activity of polysaccharide from Coprinus comatus.
The antioxidant activity in vitro and vivo of polysaccharide from Coprinus comatus were tested and compared with those of VC. The results showed that the polysaccharides from Coprinus comatus had good scavenging effect on ·OH and NO·, moreover, the scavenging rate of DPPS(deproteinated) were higher than CPS(non-deproteinated,crude). The ·OH and NO· radical scavenging rate could reach 62.1%, 70.1% for DPPS and 44% and 63.1% for CPS respectively when the concentration of polysaccharide was 8 mg/mL. The good scavenging effect of DPPS and CPS on·DPPH was also proved with 62.55% and 48.4% respectively. Also, the polysaccharide from Coprinus comatus had a certain scavenging effect on O2-·. Compared with model group, the GSH-Px, SOD, CAT and T-AOC activities of DPPS treatment group were significantly raised, and the MDA content was remarkably reduced. All of this conceived the good antioxidant activity of polysaccharide from Coprinus comatus.
引文
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[12] YU L, ZHAO M, WANG J, et al. Antioxidant,immunomodulatory and anti-breast cancer activities of phenolic extract from pine(Pinus massoniana Lamb)bark[J]. J Innov Food Sci Emerg, 2008, 9(1):122-128.
[13] STEWAR R C, BEEWLEY J D. Lipid peroxidation associated with accelerated aging of soybean areas[J].Plant Phys, 1980, 65(2):245-248.
[14]马钊.金针菇SF-07菌株多糖的提取、结构与抗氧化活性分析[D].泰安:山东农业大学, 2015.
[15]孙汉巨.菜籽多糖的分离、纯化及特性研究[D].合肥:合肥工业大学, 2009.
[16]张云侠.平菇多糖的提取、鉴定及其抗氧化活性研究[D].合肥:安徽大学, 2012.
[17]程光宇,刘俊,王峰,等.鸡腿菇子实体多糖对小鼠血液和肝脏的抗氧化作用[J].食品科学, 2010,31(13):267-272.
[2]王岩,关洪全,马贤德.毛头鬼伞多糖对免疫抑制小鼠体液免疫功能的影响[J].吉林中医药, 2010, 30(3):260-261.
[3] JIANG X G, LIAN M X, HAN Y, et al. Retraction notice to “Antitumor and immunomodulatory activity of a polysaccharide from fungus Coprinus comatus(Mull.:Fr.)Gray”[J]. Int J Biol Macromol, 2013,58:349-353.
[4]崔鹏举.富硒毛头鬼伞菌丝深层发酵培养及其降血糖活性研究[D].汕头:汕头大学, 2009.
[5] MWITA L N, MSHANDETE A M, LYANTAGAYE S L. Improved antimicrobial activity of the Tanzanian edible mushroom Coprinus cinereus(Schaeff)Gray by chicken manure supplemented solid sisal wastes substrates[J]. J Yeast Fungal Res, 2010, 1(10):201-206.
[6]王金玺,顾林,孔凡伟,等.鸡腿菇粗多糖的体外抗氧化性[J].食品科学, 2012, 33(13):79-82.
[7] LI B, LU F, SUO X, et al. Antioxidant properties of cap and stipe from Coprinus comatus[J]. Molecules,2010, 15:1473-1486.[8吴艳兵,谢荔岩,谢联辉,等.毛头鬼伞(Coprinus comatus)多糖的理化性质及体外抗氧化活性[J].激光生物学报, 2007, 16(4):438-442.
[9]陈丹红,陈南.分光光度法研究鸡腿菇多糖的抗氧化性及抗菌性[J].福建轻纺, 2010, 12:20-23.
[10] OYAIZU M. Antioxidative activity of browning products of glucosamine fractionated by organic solvent and thin layer chromatography[J]. Jpn J Nutri. 1986,35:771-775.
[11] SMIRONFF N, CUMBES Q J. Hyroxyl radical scavenging activity of compatible solutes[J]. Phytochemistry, 1989, 28(4):1057-1060.
[12] YU L, ZHAO M, WANG J, et al. Antioxidant,immunomodulatory and anti-breast cancer activities of phenolic extract from pine(Pinus massoniana Lamb)bark[J]. J Innov Food Sci Emerg, 2008, 9(1):122-128.
[13] STEWAR R C, BEEWLEY J D. Lipid peroxidation associated with accelerated aging of soybean areas[J].Plant Phys, 1980, 65(2):245-248.
[14]马钊.金针菇SF-07菌株多糖的提取、结构与抗氧化活性分析[D].泰安:山东农业大学, 2015.
[15]孙汉巨.菜籽多糖的分离、纯化及特性研究[D].合肥:合肥工业大学, 2009.
[16]张云侠.平菇多糖的提取、鉴定及其抗氧化活性研究[D].合肥:安徽大学, 2012.
[17]程光宇,刘俊,王峰,等.鸡腿菇子实体多糖对小鼠血液和肝脏的抗氧化作用[J].食品科学, 2010,31(13):267-272.