Study on Efficient Multiplication of Blueberry (Vaccinium corymbosum) Plantlets in Swinging type Temporary Immersion Bioreactors System (S-TIBs)
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摘要
The swinging type temporary immersion bioreactors system( S-TTBs) is a kind of new and advanced method of tissue culture. The efficient multiplication technology of blueberry( Vaccinium corymbosum) plantlets in S-TTBs was systematically studied through L_9( 3~4) orthogonal experiment with three factors( culture medium volume,swing angle,and inoculation density) and completely randomized experiment with two factors( swing frequency and immersion time).The results showed that the optimal culture parameters were set as follows: the culture medium volume of 250 ml/bottle,the swing angle at 45°,and the inoculation density of 60 plantlets/bottle. The optimal swing frequency was 1 time/6 h,and the immersion time was set as 60 s.
The swinging type temporary immersion bioreactors system( S-TTBs) is a kind of new and advanced method of tissue culture. The efficient multiplication technology of blueberry( Vaccinium corymbosum) plantlets in S-TTBs was systematically studied through L_9( 3~4) orthogonal experiment with three factors( culture medium volume,swing angle,and inoculation density) and completely randomized experiment with two factors( swing frequency and immersion time).The results showed that the optimal culture parameters were set as follows: the culture medium volume of 250 ml/bottle,the swing angle at 45°,and the inoculation density of 60 plantlets/bottle. The optimal swing frequency was 1 time/6 h,and the immersion time was set as 60 s.
The swinging type temporary immersion bioreactors system( S-TTBs) is a kind of new and advanced method of tissue culture. The efficient multiplication technology of blueberry( Vaccinium corymbosum) plantlets in S-TTBs was systematically studied through L_9( 3~4) orthogonal experiment with three factors( culture medium volume,swing angle,and inoculation density) and completely randomized experiment with two factors( swing frequency and immersion time).The results showed that the optimal culture parameters were set as follows: the culture medium volume of 250 ml/bottle,the swing angle at 45°,and the inoculation density of 60 plantlets/bottle. The optimal swing frequency was 1 time/6 h,and the immersion time was set as 60 s.
引文
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[17]XU YL,ZHANG JM.A efficient large-scale tissue culture method-temporary immersion culture method[J].Plant Physiology Communications,2013,49(4):392-399.(in Chinese)
[18]ZHANG ZZ,SHI K,YU ZR,et al.Research on rapid propagation of strawberry based on plant micropropagation bioreactor[J].Chinese Agricultural Science Bulletin,2014,30(16):216-220.(in Chinese)
[19]SHI K,ZHENG CX.Liquid culture system of rapid propagation of Cotinus coggygria‘royal purple’[J].Plant Physiology Communications,2015,51(4):553-558.(in Chinese)
[20]GAO MP,LI ZC,ZHANG C,et al.Efficient propagation of Eleocharis dulcis plantlets in temporary immersion bioreactors(TIBs)[J].Guangxi Agricultural Sciences,2016,47(10):1653-1657.(in Chinese)
[21]MEHROTRA S,GOEL MK,KUKREJA AK,et al.Efficiency of liquid culture system over conventional micropropagation:A progress towards commercialization[J].African Journal of Biotechnology,2007,6(13):1484-1492.
[2]BORNSEK SM,ZIBERNA L,POLAK T,et al.Bilberry and blueberry anthocyanins act as powerful intracellular antioxidants in mammalian cells[J].Food Chem,2012,134(4):1878-1884.(in Chinese)
[3]ZHAO XL.Research progress of ingredient and healthy function of blueberry[J].Chinese Wild Plant Resources,2011,30(6):19-23
[4]WANG SS,SUN AD,LI SY.Advancement of health function of blueberry and its utilization[J].?Food and Nutrition in China,2010(6):17-20.(in Chinese)
[5]WU X,RAHAL O,KANG J,et al.In utero and lactational exposure to blueberry via maternal diet promotes mammary epithelial differentiation in prepubescent female rats[J].Nutr Res,2009,29(11):802-811
[6]DEL BO C,MARTINI D,VENDRAME S,et al.Improvement of lymphocyte resistance against H2O2-induced DNA damage in Sprague-Dawley rats after eight weeks of a wild blueberry(Vaccinium angustifolium)-enriched diet[J].Mutat Res-Genet Toxicol Environ Mutagen,2010,703(2):158-162
[7]LACOMBE A,WU VCH,WHITE J,et al.The antimicrobial properties of the lowbush blueberry(Vaccinium angustifolium)fractional compo- nents against foodborne pathogens and the conservation of probiotic Lactobacillus rhamnosus[J].Food Microbiol,2012,30(1):124-131
[8]KADER F,ROVEL B,GIRARDIN M,METCHE M.Fractionation and identification of the phenolic compounds of Highbush blueberries(Vaccinium corgmbosum L.)[J].Food Chem,1996,55(1):35-40
[9]GAO MP,LI ZC,ZHANG C,et al.Efficient propagation of Eleocharis dulcis plantlets in temporary immersion bioreactors(TIBs)[J].Guangxi Agricultural Sciences,2016,47(10):1653-1657.(in Chinese)
[10]ZHAO WF,WANG LH.Study on temporary immersion bioreactor in tissue culture[J].Journal of Anhui Agricultural Sciences,2007,35(2):317-320,323.(in Chinese)
[11]MEYER GM,BANASIAK M,NTOYI TT,et al.Sugarcane plants from temporary immersion culture:acclimating for commercial production[J].Acta Hortic,2009,812:323-327
[12]PEЙA-RAMREZ YJ,JUREZ-GMEZ J,GMEZ-LPEZ L,et al.Multiple adventitious shoot formation in Spanish red cedar(Cedrela odorata L.)cultured in vitro using juvenile and mature tissues:an improved micropropagation protocol for a highly valuable tropical tree species[J].In Vitro Cell Dev-Pl,2010,46:149-160
[13]SNYMAN SJ,MEYER GM,KOCH AC,et al.Applications of in vitro culture systems for commercial sugarcane production and improvement[J].In Vitro Cell Dev-Pl,2011a,47:234-249
[14]YANG L,QIN G,SU J,et al.Micro-propagation of banana(Musa AAA Cavendish Var.Williams B6)in Temporary immersion bioreactor system(TIBS)[J].Journal of Fruit Science,2010b,27(6):1010-1013.(in Chinese)
[15]YANG L,QIN G,YANG LT,et al.Optimization of Saccharum sinensis Roxb rapid multiplication in temporary immersion bioreactors system[J].Chinese Journal of Tropical Crops,2010a,31(4):614-620.(in Chinese)
[16]YANG L,QIN G,YANG LT,et al.Optimization of sugarcane rapid propagation in temporary immersion bioreactors system[J].Journal of South China Agricultural University,2011,32(1):37-41.(in Chinese)
[17]XU YL,ZHANG JM.A efficient large-scale tissue culture method-temporary immersion culture method[J].Plant Physiology Communications,2013,49(4):392-399.(in Chinese)
[18]ZHANG ZZ,SHI K,YU ZR,et al.Research on rapid propagation of strawberry based on plant micropropagation bioreactor[J].Chinese Agricultural Science Bulletin,2014,30(16):216-220.(in Chinese)
[19]SHI K,ZHENG CX.Liquid culture system of rapid propagation of Cotinus coggygria‘royal purple’[J].Plant Physiology Communications,2015,51(4):553-558.(in Chinese)
[20]GAO MP,LI ZC,ZHANG C,et al.Efficient propagation of Eleocharis dulcis plantlets in temporary immersion bioreactors(TIBs)[J].Guangxi Agricultural Sciences,2016,47(10):1653-1657.(in Chinese)
[21]MEHROTRA S,GOEL MK,KUKREJA AK,et al.Efficiency of liquid culture system over conventional micropropagation:A progress towards commercialization[J].African Journal of Biotechnology,2007,6(13):1484-1492.