酪蛋白源七肽AVPYPQR在不同体系中抗氧化活性的构效关系比较
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摘要
本文为了探讨酪蛋白源七肽AVPYPQR在不同体系中的抗氧化活性及构效关系,比较研究了AVPYPQR及其相关片段PYPQ、PYPQR、VPYPQ、VPYPQR和AVPYPQ在体外化学方法和细胞模型中的抗氧化能力。结果表明,在ABTS自由基清除能力和氧自由基吸收能力(ORAC)等体外化学方法中6条肽的活性都高于标准抗氧化剂Trolox,其中Val、Ala和Arg残基的同时存在降低AVPYPQR的ABTS自由基清除能力,而Arg残基存在提升了AVPYPQR的ORAC活性。在细胞氧化损伤模型中,6条多肽对AAPH诱导血红细胞溶血都有保护作用,其中AVPYPQR保护效果最好,Ala和Arg残基存在提升其保护效果;H2O2诱导Hep G2细胞氧化损伤模型中,除PYPQ,其他多肽都具有显著保护效果(p<0.05),其中Ala、Val和Arg残基都可以提升保护效果,但是没有叠加作用。综上,除PYPQ,其它5条多肽在在体外化学方法和细胞模型中都表现出较强抗氧化活性,但无显著性相关,且不同方法中Val、Ala和Arg残基对AVPYPQR活性影响不同。
In order to investigate the antioxidant activity and structure-activity relationship of casein-derived heptapeptide AVPYPQR in different systems, the antioxidant activity of AVPYPQR and its related fragments PYPQ, PYPQR, VPYPQ, VPYPQR and AVPYPQ were compared in this study using in vitro chemical assays and cellular models. The results showed that the six peptides exhibited higher ABTS free radical scavenging activity and oxygen radical absorbance capacity(ORAC) than the standard antioxidant, Trolox. The co-occurrence of Val, Ala and Arg residues led to a reduced ABTS radical scavenging activity of AVPYPQR, while the presence of Arg residue resulted in an enhanced ORAC. In the cell oxidative damage model, all the six peptides offered protection against AAPH-induced erythrocyte hemolysis. Among which,AVPYPQR exhibited the greatest protective effect, and the presence of Ala and Arg residues improved further such an effect. In H2 O2-induced Hep G2 cell oxidative damage model, all the peptides showed(p < 0.05) significant protective effect except for PYPQ, and the Ala, Val and Arg residues could enhance the protection but without an addictive effect. In summary, all the peptides, except for PYPQ, had strong antioxidant activity in both in vitro chemical assays and cellular models, but no significant correlation was found among the detected activities in different assays. The influence of Val, Ala and Arg residues on the antioxidant activity of AVPYPQR varied between analysis assays.
In order to investigate the antioxidant activity and structure-activity relationship of casein-derived heptapeptide AVPYPQR in different systems, the antioxidant activity of AVPYPQR and its related fragments PYPQ, PYPQR, VPYPQ, VPYPQR and AVPYPQ were compared in this study using in vitro chemical assays and cellular models. The results showed that the six peptides exhibited higher ABTS free radical scavenging activity and oxygen radical absorbance capacity(ORAC) than the standard antioxidant, Trolox. The co-occurrence of Val, Ala and Arg residues led to a reduced ABTS radical scavenging activity of AVPYPQR, while the presence of Arg residue resulted in an enhanced ORAC. In the cell oxidative damage model, all the six peptides offered protection against AAPH-induced erythrocyte hemolysis. Among which,AVPYPQR exhibited the greatest protective effect, and the presence of Ala and Arg residues improved further such an effect. In H2 O2-induced Hep G2 cell oxidative damage model, all the peptides showed(p < 0.05) significant protective effect except for PYPQ, and the Ala, Val and Arg residues could enhance the protection but without an addictive effect. In summary, all the peptides, except for PYPQ, had strong antioxidant activity in both in vitro chemical assays and cellular models, but no significant correlation was found among the detected activities in different assays. The influence of Val, Ala and Arg residues on the antioxidant activity of AVPYPQR varied between analysis assays.
引文
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[2]Somogyi A O,Kl A R,P Eter Pusztai,et al.Antioxidant measurements[J].Physiological Measurement,2007,28:R41-R55
[3]Zou T,He T,Li H,et al.The structure-activity relationship of the antioxidant peptides from natural proteins[J].Molecules,2016,21(1):72
[4]郑淋.抗氧化肽的构效关系及定向制备的研究[D].广州:华南理工大学,2015ZHENG Lin.Structure-activity relationship and directed preparation of antioxidant peptides[D].Guangzhou:South China University of Technology,2015
[5]Wu R,Wu C,Liu D,et al.Overview of antioxidant peptides derived from marine resources:the sources,characteristic,purification,and evaluation methods[J].Applied Biochemistry and Biotechnology,2015,176(7):1815-1833
[6]Power O,Jakeman P,FitzGerald R J.Antioxidative peptides:enzymatic production,in vitro and in vivo antioxidant activity and potential applications of milk-derived antioxidative peptides[J].Amino Acids,2013,44(3):797-820
[7]Liang R,Zhang Z,Lin S.Effects of pulsed electric field on intracellular antioxidant activity and antioxidant enzyme regulating capacities of pine nut(Pinus Koraiensis)peptide QDHCH in Hep G2 cells[J].Food Chemistry,2017,237:793-802
[8]Zhang X,Wang L,Wang R,et al.Protective effects of rice dreg protein hydrolysates against hydrogen peroxide-induced oxidative stress in HepG-2 cells[J].Food&Function,2016,7(3):1429-1437
[9]Phrueksanan W,Yibchok-anun S,Adisakwattana S.Protection of Clitoria ternatea flower petal extract against free radical-induced hemolysis and oxidative damage in canine erythrocytes[J].Research in Veterinary Science,2014,97(2):357-363
[10]Yang H,Korivi M,Lin M,et al.Antihemolytic and antioxidant properties of pearl powder against 2,2’-azobis(2-amidinopropane)dihydrochloride-induced hemolysis and oxidative damage to erythrocyte membrane lipids and proteins[J].Journal of Food and Drug Analysis,2017,25(4):898-907
[11]Zheng L,Zhao Y J,Dong H Z,et al.Structure-activity relationship of antioxidant dipeptides:Dominant role of Tyr,Trp,Cys and Met residues[J].Journal of Functional Foods,2016,21:485-496
[12]Ohashi Y,Onuma R,Naganuma T,et al.Antioxidant properties of tripeptides revealed by a comparison of six different assays[J].Food Science&Technology International Tokyo,2015,21(5):695-704
[13]Sabahi Z,Soltani F,Moein M.Insight into DNA protection ability of medicinal herbs and potential mechanisms in hydrogen peroxide damages model[J].Asian Pacific Journal of Tropical Biomedicine,2018,8(2):120
[14]Tonolo F,Sandre M,Ferro S,et al.Milk-derived bioactive peptides protect against oxidative stress in a Caco-2 cell model[J].Food&Function,2018,9(2):1245-1253