硫酸软骨素蛋白多糖4在口腔鳞状细胞癌组织中的表达及其临床意义
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摘要
目的:研究硫酸软骨素蛋白多糖4(CSPG4)在口腔鳞状细胞癌(OSCC)组织中的表达及其临床意义。方法:应用荧光定量PCR及免疫组织化学技术检测42例OSCC组织及相应的癌旁正常组织(>2cm)中CSPG4mRNA及其蛋白的表达,并分析其与患者临床特征之间的关系。结果:CSPG4 mRNA及其蛋白在OSCC组织中的表达均较癌旁组织显著上调(均P<0.05),CSPG4mRNA及其蛋白的表达与患者年龄、民族、OSCC分化程度无关(P>0.05),与性别、淋巴结转移及临床分期相关,男性患者中的表达较女性患者高,转移组高于非转移组,Ⅲ～Ⅳ期高于Ⅰ～Ⅱ期(均P<0.05)。结论:CSPG4与OSCC的发生、发展及转移有着密切的联系。
Objective:To investigate the expression of chondrioditin sulfate proteoglycans 4(CSPG4)and its clinical significance in oral squamous cell carcinoma(OSCC).Methods:Fluorescence quantitative PCR and immunohistochemical staining were respectively performed to detect the mRNA and protein expressions of CSPG4 in OSCC tissues and corresponding adjacent tissues.The relationship between the CSPG4 expression and patients' clinicopathological features was analyzed.Results:The expression of CSPG4 mRNA and protein in OSCC tissues was significantly higher than that in adjacent tissues(P<0.05).There was no relationship between the CSPG4 expression and age,ethnicity and OSCC differentiation degree(P>0.05).The expression of CSPG4 was strongly correlated with the gender,lymph node metastasis and clinical stage.Up-regulated CSPG4 correlated with a higher clinical stage and lymph node metastasis and was more common in males(P < 0.05).Conclusion:The expression of CSPG4 was closely linked to the occurrence and development of OSCC.
Objective:To investigate the expression of chondrioditin sulfate proteoglycans 4(CSPG4)and its clinical significance in oral squamous cell carcinoma(OSCC).Methods:Fluorescence quantitative PCR and immunohistochemical staining were respectively performed to detect the mRNA and protein expressions of CSPG4 in OSCC tissues and corresponding adjacent tissues.The relationship between the CSPG4 expression and patients' clinicopathological features was analyzed.Results:The expression of CSPG4 mRNA and protein in OSCC tissues was significantly higher than that in adjacent tissues(P<0.05).There was no relationship between the CSPG4 expression and age,ethnicity and OSCC differentiation degree(P>0.05).The expression of CSPG4 was strongly correlated with the gender,lymph node metastasis and clinical stage.Up-regulated CSPG4 correlated with a higher clinical stage and lymph node metastasis and was more common in males(P < 0.05).Conclusion:The expression of CSPG4 was closely linked to the occurrence and development of OSCC.
引文
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[16]KASTEN B B,OLIVER P G,KIM H,et al.(212)Pblabeled antibody 225.28targeted to chondroitin sulfate proteoglycan 4for triple-negative breast cancer therapy in mouse models[J].International Journal of Molecular Sciences,2018,19(4):925-940.
[17]SIMON B,HARRER D C,SCHULER-THURNER B,et al.The siRNA-mediated downregulation of PD-1alone or simultaneously with CTLA-4shows enhanced in-vitro CAR-T cell functionality for further clinical development towards the potential use in immunotherapy of melanoma[J].Experimental Dermatology,2018,27(7):769-778.
[2] MA J,TANG X,SUN W W,et al.Mutant GDF15presents a poor prognostic outcome for patients with oral squamous cell carcinoma[J].Oncotarget,2016,7(2):2113-2122.
[3] MITSOU I,MULTHAUPT HAB,COUCHMAN J R.Proteoglycans,ion channels and cell-matrix adhesion[J].The Biochemical Journal,2017,474(12):1965-1979.
[4] GARUSI E,ROSSI S,PERRIS R.Antithetic roles of proteoglycans in cancer[J].Cellular and Molecular Life Sciences,2012,69(4):553-579.
[5] WANG J,SVENDSEN A,KMIECIK J,et al.Targeting the NG2/CSPG4proteoglycan retards tumour growth and angiogenesis in preclinical models of GBM and melanoma[J].PLoS One,2011,6(7):e23062.
[6] WU Y,ZHOU B P.Snail:More than EMT.Cell adhesion&migration[J].Apr-Jun,2010,4(2):199-203.
[7] WUSHOU A,HOU J,ZHAO Y J,et al.Twist-1upregulation in carcinoma correlates to poor survival.[J].International Journal of Molecular Sciences,2014,15(12):21621-21630.
[8] MAROCCHIO L S,LIMA J,SPERANDIO F F,et al.Oral squamous cell carcinoma:an analysis of 1,564cases showing advances in early detection[J].Journal of Oral Science,2010,52(2):267-273.
[9]李占,魁柯,倪黎明,等.雄激素对HIBD新生鼠phosphacan和NG_2蛋白聚糖表达与轴突再生的影响研究[J].中国当代儿科杂志,2008(3):357-361.
[10]SHAPIRO J P,GUZELOGLU-KAYISLI O,KAYISLI U A,et al.Thrombin impairs human endometrial endothelial angiogenesis;implications for progestin-only contraceptive-induced abnormal uterine bleeding[J].Contraception,2017,95(6):592-601.
[11]TSIDULKO A Y,KAZANSKAYA G M,KOSTROMSKAYA D V,et al.Prognostic relevance of NG2/CSPG4,CD44and Ki-67in patients with glioblastoma[J].Journal of the International Society for Oncodevelopmental Biology and Medicine,2017,39(9):1010428317724282.
[12]SCHOENFELD A J,WANG X,WANG Y,et al.CSPG4as a prognostic biomarker in chordoma[J].Official Journal of the North American Spine Society,2016,16(6):722-727.
[13]KELEG S,TITOV A,HELLER A,et al.Chondroitin sulfate proteoglycan CSPG4as a novel hypoxia-sensitive marker in pancreatic tumors[J].PLoS One,2014,9(6):e100178.
[14]CATTARUZZA S,NICOLOSI PA,BRAGHETTA P,et al.NG2/CSPG4-collagen type VI interplays putatively involved in the microenvironmental control of tumour engraftment and local expansion[J].Journal of Molecular Cell Biology,2013,5(3):176-193.
[15] WINSHIP A,VAN SINDEREN M,HEFFERNANMARKS A,et al.Chondroitin sulfate proteoglycan protein is stimulated by interleukin 11and promotes endometrial epithelial cancer cell proliferation and migration[J].International Journal of Oncology,2017,50(3):798-804.
[16]KASTEN B B,OLIVER P G,KIM H,et al.(212)Pblabeled antibody 225.28targeted to chondroitin sulfate proteoglycan 4for triple-negative breast cancer therapy in mouse models[J].International Journal of Molecular Sciences,2018,19(4):925-940.
[17]SIMON B,HARRER D C,SCHULER-THURNER B,et al.The siRNA-mediated downregulation of PD-1alone or simultaneously with CTLA-4shows enhanced in-vitro CAR-T cell functionality for further clinical development towards the potential use in immunotherapy of melanoma[J].Experimental Dermatology,2018,27(7):769-778.