青海湖裸鲤和花斑裸鲤消化酶活力对比
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摘要
本实验对比研究青海湖裸鲤(Gymnocypris przewalskii)和花斑裸鲤(Gymnocypris eckloni)消化酶活性差异。两种鱼各挑选9尾体重100g左右的鱼作为实验对象,分别检测肝胰脏、前肠、中肠及后肠中的脂肪酶、淀粉酶、糜蛋白酶和胰蛋白酶活性。结果表明:作为无胃鱼,两种鱼肠道消化酶活性显著高于肝胰脏,其中前肠是主要的消化器官,后肠具有较高的蛋白质消化能力。通过对比研究发现青海湖裸鲤具有较高的脂肪和糖类消化能力,而花斑裸鲤具有较高的蛋白质消化能力。
Differences of digestive enzymes activities between Gymnocypris przewalskii and Gymnocypris eckloni were analyzed.Hepatopancreas,foregut,midgut and hindgut of nine fish for each species(body weight:100 g)were sampled to analyze the following enzymes:trypsin,chymotrypsin,lipase and amylase.Results showed that as stomachless fish,all digestive enzymes activites in intestine were higher than those in hepatopancreas for the two fish species.Foreguts of two fish species contributed more in nutrient digestion,hindguts of those were also the main sites for protein digestion.Gymnocypris przewalskii had the higher ablility to digest fat and carbohydrate,however,Gymnocypris eckloni had the higher ablility to digest protein.
Differences of digestive enzymes activities between Gymnocypris przewalskii and Gymnocypris eckloni were analyzed.Hepatopancreas,foregut,midgut and hindgut of nine fish for each species(body weight:100 g)were sampled to analyze the following enzymes:trypsin,chymotrypsin,lipase and amylase.Results showed that as stomachless fish,all digestive enzymes activites in intestine were higher than those in hepatopancreas for the two fish species.Foreguts of two fish species contributed more in nutrient digestion,hindguts of those were also the main sites for protein digestion.Gymnocypris przewalskii had the higher ablility to digest fat and carbohydrate,however,Gymnocypris eckloni had the higher ablility to digest protein.
引文
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[12]Stroband HW,Kroon AG.The development of the stomach in Clarias lazera and the intestinal absorption of protein macromolecules[J].Cell&Tissue Research,1981,215:397-415.
[13]欧阳冬冬,佘长宁,郭仁湘,等.云斑尖塘鳢消化酶活力的研究[J].激光生物学报,2016(25):431-436.
[2]Xiong DM,Xie CX,Zhang HJ,et al.Digestive enzymes along digestive tract of a carnivorous fish Glyptosternum maculatum(Sisoridae,Siluriformes)[J].Journal of Animal Physiology and Animal Nutrition,2011,95:56-64.
[3]史建全,刘建虎,陈大庆,等.青海湖裸鲤肠道组织学研究[J].淡水渔业,2004,34:16-19.
[4]Hidalgo MC,Urea E,Sanz A.Comparative study of digestive enzymes in fish with different nutritional habits.Proteolytic and amylase activities[J].Aquaculture,1999,170:267-283.
[5]黄瑾,熊邦喜,陈洁,等.匙吻鲟的消化酶分布及其活性[J].华中农业大学学报,2013(32):110-115.
[6]Uys W,Hecht T.Assays on the digestive enzymes of sharptooth catfish,Clarias gariepinus(Pisces:Clariidae)[J].Aquaculture,1987,63:301-313.
[7]Deguara S,Jauncey K,Agius C.Enzyme activities and pH variations in the digestive tract of gilthead sea bream[J].Journal of Fish Biology,2003,62:1033-1043.
[8]Tengjaroenkul B,Smith BJ,Caceci T,et al.Distribution of intestinal enzyme activities along the intestinal tract of cultured Nile tilapia,Oreochromis niloticus L[J].Aquaculture,2000,182:317-327.
[9]Tramati C,Savona B,Mazzola A.A study of the pattern of digestive enzymes in Diplodus puntazzo(Cetti,1777)(Osteichthyes,Sparidae):evidence for the definition of nutritional protocols[J].Aquaculture International,2005,13:89-95.
[10]Lundstedt LM,Fernando Bibiano Melo J,Moraes G.Digestive enzymes and metabolic profile of Pseudoplatystoma corruscans(Teleostei:Siluriformes)in response to diet composition[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,2004,137:331-339.
[11]Castro C,Couto A,Pérez-Jiménez A,et al.Effects of fish oil replacement by vegetable oil blend on digestive enzymes and tissue histomorphology of European sea bass(Dicentrarchus labrax)juveniles[J].Fish Physiology and Biochemistry,2016,42:203-217.
[12]Stroband HW,Kroon AG.The development of the stomach in Clarias lazera and the intestinal absorption of protein macromolecules[J].Cell&Tissue Research,1981,215:397-415.
[13]欧阳冬冬,佘长宁,郭仁湘,等.云斑尖塘鳢消化酶活力的研究[J].激光生物学报,2016(25):431-436.