泰国输华真柏中一种隐皮孢囊线虫的鉴定
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摘要
深圳口岸从来自泰国真柏苗木中截获一种隐皮孢囊线虫。序列分析显示截获的孢囊线虫28S r DNA-D2/D3区基因序列与Gen Bank中布林克曼隐皮孢囊线虫(Cryphodera brinkmani)(登录号为JQ965677和DQ328705)的序列相似性为99%。系统进化树中截获的孢囊线虫和布林克曼隐皮孢囊线虫聚为一个分支。结合形态学特征比较和分子特征分析,将截获的孢囊线虫鉴定为布林克曼隐皮孢囊线虫。这是我国口岸首次报道截获该线虫。
The Cryphodera sp. was detected in roots of Juniperus chinensis from Thailand at Shenzhen port. 28 S r DNA-D2/D3 region sequence analysis showed 99% identity with Cryphodera brinkmani in GenBank(accession No. JQ965677 and DQ328705). The phylogenetic tree based on 28 S r DNA-D2/D3 region revealed that the intercepted nematode clustered with C.brinkmani. According to the results of morphological and molecular characteristics,the intercepted cyst nematode was identified as C.brinkmani. This is the first report of its interception in China.
The Cryphodera sp. was detected in roots of Juniperus chinensis from Thailand at Shenzhen port. 28 S r DNA-D2/D3 region sequence analysis showed 99% identity with Cryphodera brinkmani in GenBank(accession No. JQ965677 and DQ328705). The phylogenetic tree based on 28 S r DNA-D2/D3 region revealed that the intercepted nematode clustered with C.brinkmani. According to the results of morphological and molecular characteristics,the intercepted cyst nematode was identified as C.brinkmani. This is the first report of its interception in China.
引文
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[12]顾建锋,王江岭,邵芳,等.日本鸡爪槭中苹果根结线虫的鉴定.植物检疫,2013,27(1):43-49.
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[15]Karssen G,Van Aelst A.Description of Cryphodera brinkmani n.sp.(Nematoda:Heteroderidae),a parasite of Pinus thunbergii Parlatore from Japan,including a key to the species of the genus Cryphodera Colbran,1966.Nematology,1999,1(2):121-130.
[16]Vovlas N,Trisciuzzi N,Troccoli A,et al.Integrative diagnosis and parasitic habits of Cryphodera brinkmani a non-cyst forming heteroderid nematode intercepted on Japanese white pine bonsai trees imported into Italy.European Journal of Plant Pathology,2013,135(4):717-726.
[2]谢辉.植物线虫分类学.合肥:安徽科学技术出版,2000.
[3]Wouts W M.A revision of the family Heteroderidae(Nematoda:Tylenchoidea)II.The subfamily Meloidoderinae.Nematologica,1973,19:218-235.
[4]Baldwin J G,Mundo-Ocampo M,Othman A A.Cryphodera utahensis n.sp.(Heteroderidae),a new species from wild rose in Utah.Journal of Nematology,1983,15(2):182-191.
[5]Bajaj H K,Walia R K,Dabur K R,et al.Cryphodera kalesari,a new heteroderid nematode species from Haryana,India.Systematic Parasitology,1989,14(2):113-116.
[6]Hirata K,Yuhara I.Plant parasitic nematodes associated with mulberry plants in Gunma Prefecturd.Japanese Journal of Nematology,1972,2:20-22.
[7]Toida Y.Nematode species from mulberry fields and their geographical distribution in Japan.Japanese Journal of Nematology,1984,14:20-27.
[8]Hirata K,Yuhara I.Plant parasitic nematodes detected from bonsai trees and bonsai nurseries.Research Bulletin of the Plant Protection Service,Japana,1986,22:111–117.
[9]Subbotin S A,Mundo-Ocampo M,Baldwin J G.Systematics of Cyst Nematodes(Nematoda:Heteroderinae),Volume8 Part A.Leiden,the Netherlands:Brill,2010:285-290.
[10]Reddy P P.Plant Nematology.New Delhi:Pratibha Printing Press,1983.
[11]Long H,Liu H,Xu J H.Development of a PCR Diagnostic for the Root-knot Nematode Meloidogyne enterolobii.Acta Phytopathologica Sinica,2006,36(2):109-115.
[12]顾建锋,王江岭,邵芳,等.日本鸡爪槭中苹果根结线虫的鉴定.植物检疫,2013,27(1):43-49.
[13]Hall T A.Bio Edit:a user-friendly biological sequence alignment editor and analysis program for windows 95/98NT.Nucleic Acids Symposium Serries,1999,41(41):95-98.
[14]Tamura K,Dudley J,Nei M,et al.MEGA4:Molecular Evolutionary Genetics Analysis(MEGA)software version 4.0.Molecular Biology and Evolution,2007,24(8):1596-1599.
[15]Karssen G,Van Aelst A.Description of Cryphodera brinkmani n.sp.(Nematoda:Heteroderidae),a parasite of Pinus thunbergii Parlatore from Japan,including a key to the species of the genus Cryphodera Colbran,1966.Nematology,1999,1(2):121-130.
[16]Vovlas N,Trisciuzzi N,Troccoli A,et al.Integrative diagnosis and parasitic habits of Cryphodera brinkmani a non-cyst forming heteroderid nematode intercepted on Japanese white pine bonsai trees imported into Italy.European Journal of Plant Pathology,2013,135(4):717-726.