进境新西兰麻中玻利维亚短体线虫的鉴定
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摘要
2013年,北京口岸入境的新西兰麻中分离到一种短体线虫,通过形态学特征以及基于核糖体r RNA-ITS区及28S r RNA-D2/D3区序列比较,证实其为玻利维亚短体线虫(Pratylenchus bolivianus Corbett,1983)。该线虫的主要形态鉴别特征为:唇区有3个唇环;平均V=80%~82%;尾末端圆而光滑。该线虫至今未在我国发现,这是国际上首次从新西兰种苗中截获。
Pratylenchus bolivianus was detected from New Zealand Phormium tenax plant. It is characterised by head with three lip annuli, V=80%-82%, female tail tip smooth and rounded. It is identified by morphological and DNA sequence analysis of the ITS region and 28 S r RNA-D2/D3 gene. This species has not been found in China, and this is the first report of its distribution in New Zealand.
Pratylenchus bolivianus was detected from New Zealand Phormium tenax plant. It is characterised by head with three lip annuli, V=80%-82%, female tail tip smooth and rounded. It is identified by morphological and DNA sequence analysis of the ITS region and 28 S r RNA-D2/D3 gene. This species has not been found in China, and this is the first report of its distribution in New Zealand.
引文
[1]Castillo P,Vovlas N.Pratylenchus(Nematoda:Pratylenchidae):Diagnosis,Biology,Pathogenicity and Management.Leiden,The Netherlands:Brill Academic Publising,2007.
[2]Inserra R N,Troccoli A,Gozel U,et al.Pratylenchus bolivianus n.sp.(Nematoda:Pratylenchidae)from amaryllis in Floridae with notes on P.scribneri and P.hexincisus.Nematology,2007,9(1):25-42.
[3]De Luca F,Troccoli A,Duncan L W,et al.Characterisation of a population of Pratylenchus bolivianus from bromeliads and description of two related new species,P.floridensis n.sp.and P.parafloridensis n.sp.,from grasses in Florida.Nematology,2010,12(6):847-868.
[4]GB/T 24829-2009毛刺线虫属(传毒种类)检疫鉴定方法.
[5]谢辉.植物线虫分类学.2版.北京:高等教育出版社,2005:30.
[6]王江岭,张建成,顾建锋.单条线虫DNA提取方法.植物检疫,2011,25(2):32-35.
[7]De Ley P,Felix M A,Frisse L M,et al.Molecular and morphological characterisation of two reproductively isolated species with mirror-image anatomy(Nemtoda:Cephalobidae).Nematology,1999,1(6):591-612.
[8]顾建锋,王江岭,何洁,等.4种常见短体线虫形态特征比较鉴别.植物检疫,2013,27(6):69-71.
[9]刘修勇.山东省短体科线虫主要属种的分类研究[D].山东:莱阳农学院,2006.
[2]Inserra R N,Troccoli A,Gozel U,et al.Pratylenchus bolivianus n.sp.(Nematoda:Pratylenchidae)from amaryllis in Floridae with notes on P.scribneri and P.hexincisus.Nematology,2007,9(1):25-42.
[3]De Luca F,Troccoli A,Duncan L W,et al.Characterisation of a population of Pratylenchus bolivianus from bromeliads and description of two related new species,P.floridensis n.sp.and P.parafloridensis n.sp.,from grasses in Florida.Nematology,2010,12(6):847-868.
[4]GB/T 24829-2009毛刺线虫属(传毒种类)检疫鉴定方法.
[5]谢辉.植物线虫分类学.2版.北京:高等教育出版社,2005:30.
[6]王江岭,张建成,顾建锋.单条线虫DNA提取方法.植物检疫,2011,25(2):32-35.
[7]De Ley P,Felix M A,Frisse L M,et al.Molecular and morphological characterisation of two reproductively isolated species with mirror-image anatomy(Nemtoda:Cephalobidae).Nematology,1999,1(6):591-612.
[8]顾建锋,王江岭,何洁,等.4种常见短体线虫形态特征比较鉴别.植物检疫,2013,27(6):69-71.
[9]刘修勇.山东省短体科线虫主要属种的分类研究[D].山东:莱阳农学院,2006.