长链非编码RNA DANCR在关节软骨损伤修复中作用机制的研究进展
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摘要
长链非编码RNA(lncRNA)是一类转录本长度超过200个核苷酸但缺乏蛋白质编码功能的RNA,在许多细胞生物过程中发挥重要的调控作用.关节软骨损伤修复一直是关节外科的热点与难点.最近研究表明lncRNA DANCR在间充质干细胞(MSCs)向软骨细胞分化和增殖过程中发挥重要作用.lncRNA DANCR可能通过断裂为miRNA、结合或竞争mRNA/miRNA、增加胞质内β-catenin等途径发挥促软骨损伤修复作用.本文就lncRNA DANCR的结构、功能及在关节软骨损伤修复过程中的可能作用机制作一综述.
Long non-coding RNA( lncRNA) is an RNA molecule that is longer than 200 nucleotides and is not translated into a protein. LncRNAs participate in various biological processes. The repair of articular cartilage damage is always a hot and difficult point in joint surgery. Recent studies show that long non- coding RNA DANCR plays a key role in chondrogenic differentitation of human mesenchymal stem cells( MSCs) by interaction with miRNAs / mRNA and increasing the content of β-catenin in the cytoplasm. This article reviews the mechanism of DANCR in enhancing chondrogenhic differentiation and proliferation of human MSCs.
Long non-coding RNA( lncRNA) is an RNA molecule that is longer than 200 nucleotides and is not translated into a protein. LncRNAs participate in various biological processes. The repair of articular cartilage damage is always a hot and difficult point in joint surgery. Recent studies show that long non- coding RNA DANCR plays a key role in chondrogenic differentitation of human mesenchymal stem cells( MSCs) by interaction with miRNAs / mRNA and increasing the content of β-catenin in the cytoplasm. This article reviews the mechanism of DANCR in enhancing chondrogenhic differentiation and proliferation of human MSCs.
引文
[1]Merceron C,Portron S,Masson M,et al.Cartilage tissue engineering:From hydrogel to mesenchymal stem cells[J].Biomed Mater Eng,2010,20(3):159-166.
[2]Koga H,Engebretsen L,Brinchmann JE,et al.Mesenchymal stem cell-based therapy for cartilage repair:a review[J].Knee Surg Sports Traumatol Arthrosc,2009,17(11):1289-1297.
[3]Mercer TR,Dinger ME,Mattick JS.Long non-coding RNAs:insights into functions[J].Nat Rev Genet,2009,10(3):155-159.
[4]Wang L,Li Z,Li Z,et al.Long noncoding RNAs expression signatures in chondrogenic differentiation of human bone marrow mesenchymal stem cells[J].Biochem Biophys Res Commun,2015,456(1):459-464.
[5]Zhang L,Chen S,Bao N,et al.Sox4 enhances chondrogenic differentiation and proliferation of human synovium-derived stem cell via activation of long noncoding RNA DANCR[J].J Mol Histol,2015,46(6):467-473.
[6]Hochberg MC,Altman RD,April KT,et al.American College of Rheumatology 2012 recommendations for the use of nonpharmacologic and pharmacologic therapies in osteoarthritis of the hand,hip,and knee[J].Arthritis Care Res(Hoboken),2012,64(4):465-474.
[7]Orth P,Rey-Rico A,Venkatesan JK,et al.Current perspectives in stem cell research for knee cartilage repair[J].Stem Cells Cloning,2014,7:1-17.
[8]Frith JE,Thomson B,Genever PG.Dynamic three-dimensional culture methods enhance mesenchymal stem cell properties and increase therapeutic potential[J].Tissue Eng Part C Methods,2010,16(4):735-749.
[9]Gómez-Barrena E,SoláCA,Bunu CP.Regulatory authorities and orthopaedic clinical trials on expanded mesenchymal stem cells[J].Int Orthop,2014,38(9):1803-1809.
[10]Djebali S,Davis CA,Merkel A,et al.Landscape of transcription in human cells[J].Nature,2012,489(7414):101-108.
[11]Cabili MN,Trapnell C,Goff L,et al.Integrative annotation of human large intergenic noncoding RNAs reveals global properties and specific subclasses[J].Genes Dev,2011,25(18):1915-1927.
[12]Fatica A,Bozzoni I.Long non-coding RNAs:new players in cell differentiation and development[J].Nat Rev Genet,2014,15(1):7-21.
[13]Hu W,Alvarez-Dominguez JR,Lodish HF.Regulation of mammalian cell differentiation by long non-coding RNAs[J].EMBO Rep,2012,13(11):971-983.
[14]Vance KW,Ponting CP.Transcriptional regulatory functions of nuclear long noncoding RNAs[J].Trends Genet,2014,30(8):348-355.
[15]Quinodoz S,Guttman M.Long noncoding RNAs:an emerging link between gene regulation and nuclear organization[J].Trends Cell Biol,2014,24(11):651-663.
[16]Rinn JL,Chang HY.Genome regulation by long noncoding RNAs[J].Annu Rev Biochem,2012,81:145-166.
[17]Kretz M,Webster DE,Flockhart RJ,et al.Suppression of progenitor differentiation requires the long noncoding RNA ANCR[J].Genes Dev,2012,26(4):338-343.
[18]Novikova IV,Hennelly SP,Tung CS,et al.Rise of the RNA machines:exploring the structure of long non-coding RNAs[J].J Mol Biol,2013,425(19):3731-3746.
[19]Yuan SX,Wang J,Yang F,et al.Long noncoding RNA DANCR increases stemness features of hepatocellular carcinoma by derepression of CTNNB1[J].Hepatology,2016,63(2):499-511.
[20]Jia J,Li F,Tang XS,et al.Long noncoding RNA DANCR promotes invasion of prostate cancer through epigenetically silencing expression of TIMP2/3[J].Oncotarget,2016,7(25):37868-37881.
[21]Liu Y,Zhang M,Liang L,et al.Over-expression of lncRNA DANCR is associated with advanced tumor progression and poor prognosis in patients with colorectal cancer[J].Int J Clin Exp Pathol,2015,8(9):11480-11484.
[22]Zhu L,Xu PC.Downregulated LncRNA-ANCR promotes osteoblast differentiation by targeting EZH2 and regulating Runx2 expression[J].Biochem Biophys Res Commun,2013,432(4):612-617.
[23]Jia Q,Jiang W,Ni L.Down-regulated non-coding RNA(lncRNAANCR)promotes osteogenic differentiation of periodontal ligament stem cells[J].Arch Oral Biol,2015,60(2):234-241.
[24]Fu M,Huang G,Zhang Z,et al.Expression profile of long noncoding RNAs in cartilage from knee osteoarthritis patients[J].Osteoarthr Cartil,2015,23(3):423-432.
[25]Huang Y,Zheng Y,Jin C,et al.Long Non-coding RNA H19 Inhibits Adipocyte Differentiation of Bone Marrow Mesenchymal Stem Cells through Epigenetic Modulation of Histone Deacetylases[J].Sci Rep,2016,6:28897.
[26]Zhang Z,Kang Y,Zhang Z,et al.Expression of microRNAs during chondrogenesis of human adipose-derived stem cells[J].Osteoarthr Cartil,2012,20(12):1638-1646.
[27]Yang B,Guo H,Zhang Y,et al.MicroRNA-145 regulates chondrogenic differentiation of mesenchymal stem cells by targeting Sox9[J].PLo S ONE,2011,6(7):e21679.
[28]Xu J,Kang Y,Liao WM,et al.MiR-194 regulates chondrogenic differentiation of human adipose-derived stem cells by targeting Sox5[J].PLo S ONE,2012,7(3):e31861.
[29]Yan C,Wang Y,Shen XY,et al.MicroRNA regulation associated chondrogenesis of mouse MSCs grown on polyhydroxyalkanoates[J].Biomaterials,2011,32(27):6435-6444.
[30]Liu Q,Hu X,Zhang X,et al.The TMSB4 Pseudogene LncRNA Functions as a Competing Endogenous RNA to Promote Cartilage Degradation in Human Osteoarthritis[J].Mol Ther,2016,24(10):1726-1733.
[31]Gong C,Maquat LE.lncRNAs transactivate STAU1-mediated mRNA decay by duplexing with 3’UTRs via Alu elements[J].Nature,2011,470(7333):284-288.
[32]Kretz M,Siprashvili Z,Chu C,et al.Control of somatic tissue differentiation by the long non-coding RNA TINCR[J].Nature,2013,493(7431):231-235.
[33]Inui A,Iwakura T,Reddi AH.Human stem cells and articular cartilage regeneration[J].Cells,2012,1(4):994-1009.
[2]Koga H,Engebretsen L,Brinchmann JE,et al.Mesenchymal stem cell-based therapy for cartilage repair:a review[J].Knee Surg Sports Traumatol Arthrosc,2009,17(11):1289-1297.
[3]Mercer TR,Dinger ME,Mattick JS.Long non-coding RNAs:insights into functions[J].Nat Rev Genet,2009,10(3):155-159.
[4]Wang L,Li Z,Li Z,et al.Long noncoding RNAs expression signatures in chondrogenic differentiation of human bone marrow mesenchymal stem cells[J].Biochem Biophys Res Commun,2015,456(1):459-464.
[5]Zhang L,Chen S,Bao N,et al.Sox4 enhances chondrogenic differentiation and proliferation of human synovium-derived stem cell via activation of long noncoding RNA DANCR[J].J Mol Histol,2015,46(6):467-473.
[6]Hochberg MC,Altman RD,April KT,et al.American College of Rheumatology 2012 recommendations for the use of nonpharmacologic and pharmacologic therapies in osteoarthritis of the hand,hip,and knee[J].Arthritis Care Res(Hoboken),2012,64(4):465-474.
[7]Orth P,Rey-Rico A,Venkatesan JK,et al.Current perspectives in stem cell research for knee cartilage repair[J].Stem Cells Cloning,2014,7:1-17.
[8]Frith JE,Thomson B,Genever PG.Dynamic three-dimensional culture methods enhance mesenchymal stem cell properties and increase therapeutic potential[J].Tissue Eng Part C Methods,2010,16(4):735-749.
[9]Gómez-Barrena E,SoláCA,Bunu CP.Regulatory authorities and orthopaedic clinical trials on expanded mesenchymal stem cells[J].Int Orthop,2014,38(9):1803-1809.
[10]Djebali S,Davis CA,Merkel A,et al.Landscape of transcription in human cells[J].Nature,2012,489(7414):101-108.
[11]Cabili MN,Trapnell C,Goff L,et al.Integrative annotation of human large intergenic noncoding RNAs reveals global properties and specific subclasses[J].Genes Dev,2011,25(18):1915-1927.
[12]Fatica A,Bozzoni I.Long non-coding RNAs:new players in cell differentiation and development[J].Nat Rev Genet,2014,15(1):7-21.
[13]Hu W,Alvarez-Dominguez JR,Lodish HF.Regulation of mammalian cell differentiation by long non-coding RNAs[J].EMBO Rep,2012,13(11):971-983.
[14]Vance KW,Ponting CP.Transcriptional regulatory functions of nuclear long noncoding RNAs[J].Trends Genet,2014,30(8):348-355.
[15]Quinodoz S,Guttman M.Long noncoding RNAs:an emerging link between gene regulation and nuclear organization[J].Trends Cell Biol,2014,24(11):651-663.
[16]Rinn JL,Chang HY.Genome regulation by long noncoding RNAs[J].Annu Rev Biochem,2012,81:145-166.
[17]Kretz M,Webster DE,Flockhart RJ,et al.Suppression of progenitor differentiation requires the long noncoding RNA ANCR[J].Genes Dev,2012,26(4):338-343.
[18]Novikova IV,Hennelly SP,Tung CS,et al.Rise of the RNA machines:exploring the structure of long non-coding RNAs[J].J Mol Biol,2013,425(19):3731-3746.
[19]Yuan SX,Wang J,Yang F,et al.Long noncoding RNA DANCR increases stemness features of hepatocellular carcinoma by derepression of CTNNB1[J].Hepatology,2016,63(2):499-511.
[20]Jia J,Li F,Tang XS,et al.Long noncoding RNA DANCR promotes invasion of prostate cancer through epigenetically silencing expression of TIMP2/3[J].Oncotarget,2016,7(25):37868-37881.
[21]Liu Y,Zhang M,Liang L,et al.Over-expression of lncRNA DANCR is associated with advanced tumor progression and poor prognosis in patients with colorectal cancer[J].Int J Clin Exp Pathol,2015,8(9):11480-11484.
[22]Zhu L,Xu PC.Downregulated LncRNA-ANCR promotes osteoblast differentiation by targeting EZH2 and regulating Runx2 expression[J].Biochem Biophys Res Commun,2013,432(4):612-617.
[23]Jia Q,Jiang W,Ni L.Down-regulated non-coding RNA(lncRNAANCR)promotes osteogenic differentiation of periodontal ligament stem cells[J].Arch Oral Biol,2015,60(2):234-241.
[24]Fu M,Huang G,Zhang Z,et al.Expression profile of long noncoding RNAs in cartilage from knee osteoarthritis patients[J].Osteoarthr Cartil,2015,23(3):423-432.
[25]Huang Y,Zheng Y,Jin C,et al.Long Non-coding RNA H19 Inhibits Adipocyte Differentiation of Bone Marrow Mesenchymal Stem Cells through Epigenetic Modulation of Histone Deacetylases[J].Sci Rep,2016,6:28897.
[26]Zhang Z,Kang Y,Zhang Z,et al.Expression of microRNAs during chondrogenesis of human adipose-derived stem cells[J].Osteoarthr Cartil,2012,20(12):1638-1646.
[27]Yang B,Guo H,Zhang Y,et al.MicroRNA-145 regulates chondrogenic differentiation of mesenchymal stem cells by targeting Sox9[J].PLo S ONE,2011,6(7):e21679.
[28]Xu J,Kang Y,Liao WM,et al.MiR-194 regulates chondrogenic differentiation of human adipose-derived stem cells by targeting Sox5[J].PLo S ONE,2012,7(3):e31861.
[29]Yan C,Wang Y,Shen XY,et al.MicroRNA regulation associated chondrogenesis of mouse MSCs grown on polyhydroxyalkanoates[J].Biomaterials,2011,32(27):6435-6444.
[30]Liu Q,Hu X,Zhang X,et al.The TMSB4 Pseudogene LncRNA Functions as a Competing Endogenous RNA to Promote Cartilage Degradation in Human Osteoarthritis[J].Mol Ther,2016,24(10):1726-1733.
[31]Gong C,Maquat LE.lncRNAs transactivate STAU1-mediated mRNA decay by duplexing with 3’UTRs via Alu elements[J].Nature,2011,470(7333):284-288.
[32]Kretz M,Siprashvili Z,Chu C,et al.Control of somatic tissue differentiation by the long non-coding RNA TINCR[J].Nature,2013,493(7431):231-235.
[33]Inui A,Iwakura T,Reddi AH.Human stem cells and articular cartilage regeneration[J].Cells,2012,1(4):994-1009.