摘要
目的探讨子宫内膜癌组织中DAPK3和c-Myc的表达情况、相互关系及其对患者预后的影响。方法运用免疫组织化学SP法检测132例子宫内膜癌组织中DAPK3和c-Myc的表达,分析DAPK3、c-Myc蛋白的表达与子宫内膜癌临床病理参数的关系及对子宫内膜癌预后的影响。结果(1)132例组织标本中DAPK3低表达80例(80/132,60.61%),c-Myc高表达71例(71/132,53.79%);(2)DAPK3的表达在子宫内膜癌不同FIGO分级(P=0.034),组织学分级(P=0.027),淋巴结转移状态(P=0.022)和肌层浸润程度(P=0.011)中差异显著;c-Myc的表达在子宫内膜癌不同FIGO分级(P=0.015)和淋巴结转移状态(P=0.031)中差异显著;(3)生存曲线分析显示,DAPK3低表达患者的生存时间少于高表达患者(P=0.023),而c-Myc高表达患者的生存时间少于低表达患者(P=0.002);(4)等级相关分析显示DAPK3和c-Myc在子宫内膜癌中的表达显著负相关(r=-0.310,P<0.001);(5)COX回归分析提示,c-Myc高表达是子宫内膜癌患者预后的独立影响因素(P=0.007)。结论 DAPK3低表达和c-Myc高表达与子宫内膜癌的生长浸润行为相关,且与患者预后密切相关。通过检测DAPK3和c-Myc的表达能够更加有效地评估子宫内膜癌患者的预后。
Objective To investigate the expressions of DAPK3 and c-Myc and their prognostic value in endometrial carcinoma(EC). Methods The expressions of DAPK3 and c- Myc were detected immunohistochemically in 132 surgical specimens. The relationship between DAPK3 and c-Myc protein expressions and the clinicopathological features of the patients was evaluated.Results Immunohistochemical analysis revealed low DAPK3 expression in 60.61%(80/132) and high c- Myc expression in53.79%(71/132) of the specimens of EC. Both DAPK3 expression and c-Myc expression were significantly correlated with FIGO stage(P=0.034 and 0.015, respectively) and lymph node metastasis(P=0.022 and 0.031, respectively). DAPK3 expression was closely correlated with the histological grade(P=0.027) and depth of myometrial invasion(P=0.011). Kaplan- Meier survival analysis indicated that patients with low DAPK3 expressions had a shorter overall survival rate than those with high DAPK3expressions(P=0.023), while patients with high c- Myc expressions had poorer prognoses than those with low c- Myc expressions(P=0.002). Spearman correlation analysis showed that DAPK3 and c- Myc expressions were negatively correlated(P<0.001, r=-0.310). Multivariate analysis identified a high c-Myc expression as the independent predictor of the prognosis of EC(P=0.007). Conclusions A low expression of DAPK3 and a high expression of c- Myc are associated with aggressive and metastatic behaviors of EC, and their detection may help to predict the prognosis of the EC patients.
引文
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