DAPK蛋白、Ki-67蛋白在子宫内膜癌中的表达及其与临床病理学特征的相关性
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摘要
目的:探讨DAPK蛋白、Ki-67蛋白在子宫内膜癌中的表达及其与临床病理学特征的相关性。方法:选取2012年6月至2016年10月在我院及邯郸市第一医院治疗的子宫内膜癌患者150例,同时选取150例子宫内膜增生患者,采用免疫组化法检测DAPK蛋白、Ki-67蛋白表达情况,采用全自动生化法检测血清中CEA、CA19-9和CA125等的表达,分析DAPK蛋白、Ki-67蛋白的表达与子宫内膜癌临床病理特征的关系。结果:子宫内膜癌患者组织中的DAPK蛋白低于子宫内膜增生组(P<0.05),Ki-67蛋白阳性表达率均高于子宫内膜增生组患者(P<0.05);子宫内膜癌组患者CEA、CA19-9和CA125水平均高于子宫内膜增生组(P<0.05);子宫内膜癌患者的DAPK阳性表达率与CEA等肿瘤标志物水平负相关(P<0.05),子宫内膜癌患者的Ki-67阳性表达率与CEA等肿瘤标志物水平正相关(P<0.05);不同年龄的患者间DAPK、Ki-67的表达无差异,高分化、无淋巴结转移、TNM分期为Ⅰ+Ⅱ期的子宫内膜癌患者的DAPK阳性表达率较高(P<0.05),高分化、无淋巴结转移、TNM分期为Ⅰ+Ⅱ期的子宫内膜癌患者的Ki-6 7阳性表达率较低(P<0.05)。结论:DAPK蛋白、Ki-67蛋白在子宫内膜癌患者中存在异常表达,且其表达与患者的病情或者细胞分化、淋巴结转移等具有密切关系。
Objective: To investigate the correlation between DAPK protein and Ki-67 protein in endometrial carcinoma and its mechanism. Methods: From June 2012 to October 2016 with endometrial cancer patients in our hospital treatment of 150 study,and 150 cases of endometrial hyperplasia patients( control group) were detected by immunohistochemistry,DAPK protein,Ki-67 protein,expression by automatic biochemical assay of serum CEA,CA19-9 and CA125 etc. The relationship between the expression of DAPK protein,Ki-67 protein and clinical pathological characteristics of endometrial carcinoma was analysed. Results: Endometrial carcinoma tissue in DAPK protein was lower than that of control group( P < 0. 05),the expression rate of Ki-67 was higher than that in the benign group( P < 0. 05). In endometrial cancer group,CEA,CA19-9 and CA125 were significantly higher than endometrial hyperplasia group( P < 0. 05). In endometrial carcinoma patients,the positive expression rates of DAPK were negatively with CEA tumor markers( P < 0. 05),and the positive expression rates of Ki-67 were positively related to the level of tumor markers( P < 0. 05). No expression difference occured in different age between patients with DAPK,Ki-67.DAPK positive expression of high differentiation,non-lymph node metastasis,TNM stage Ⅰ + Ⅱ of the uterus endometrial cancer was higher( P < 0. 05). Ki-67 positive expression of high differentiation,non-lymph node metastasis,TNM stage Ⅰ + Ⅱ endometrial carcinoma rate was low( P < 0. 05). Conclusion: DAPK protein and Ki-67 protein show abnormal expression in patients with endometrial cancer,and its expression was correlated with the patient's condition or cell differentiation,lymph node metastasis has a close relationship,have a potential value for the development of endometrial cancer.
Objective: To investigate the correlation between DAPK protein and Ki-67 protein in endometrial carcinoma and its mechanism. Methods: From June 2012 to October 2016 with endometrial cancer patients in our hospital treatment of 150 study,and 150 cases of endometrial hyperplasia patients( control group) were detected by immunohistochemistry,DAPK protein,Ki-67 protein,expression by automatic biochemical assay of serum CEA,CA19-9 and CA125 etc. The relationship between the expression of DAPK protein,Ki-67 protein and clinical pathological characteristics of endometrial carcinoma was analysed. Results: Endometrial carcinoma tissue in DAPK protein was lower than that of control group( P < 0. 05),the expression rate of Ki-67 was higher than that in the benign group( P < 0. 05). In endometrial cancer group,CEA,CA19-9 and CA125 were significantly higher than endometrial hyperplasia group( P < 0. 05). In endometrial carcinoma patients,the positive expression rates of DAPK were negatively with CEA tumor markers( P < 0. 05),and the positive expression rates of Ki-67 were positively related to the level of tumor markers( P < 0. 05). No expression difference occured in different age between patients with DAPK,Ki-67.DAPK positive expression of high differentiation,non-lymph node metastasis,TNM stage Ⅰ + Ⅱ of the uterus endometrial cancer was higher( P < 0. 05). Ki-67 positive expression of high differentiation,non-lymph node metastasis,TNM stage Ⅰ + Ⅱ endometrial carcinoma rate was low( P < 0. 05). Conclusion: DAPK protein and Ki-67 protein show abnormal expression in patients with endometrial cancer,and its expression was correlated with the patient's condition or cell differentiation,lymph node metastasis has a close relationship,have a potential value for the development of endometrial cancer.
引文
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[11]Yang Yuan,Wang Jianliu,Wei Lihui.Notch signaling pathway and the relationship between endometriosis and endometrial cancer[J].Chinese Journal of Obstetrics and Gynecology,2015,50(5):391-393.[杨媛,王建六,魏丽惠.Notch信号通路与子宫内膜癌关系的研究进展[J].中华妇产科杂志,2015,50(5):391-393.]
[12]Zhu Minghui,Chen Ye,Wang Channi.C-erb B-2,Ki-67,ER and PR expression are correlated with the clinical pathology of endometrial carcinoma[J].Journal of Mudanjiang Medical University,2017,38(01):16-18.[朱明慧,陈烨,王婵妮.Cerb B-2、Ki-67、ER及PR表达与子宫内膜癌的临床病理相关性[J].牡丹江医学院学报,2017,38(01):16-18.]
[13]Yuan DZ,Ding XL,Yu HL,et al.Progesterone-induced Cyclin G1inhibits the proliferation of endometrial epithelial cell and its possible molecular mechanism[J].Hormone and Metabolic Research,2014,46(11):761-767.
[14]Lin C,Chen P,Wang C,et al.Antitumor effects and biological mechanism of action of the aqueous extract of the camptotheca acuminata fruit in human endometrial carcinoma cells[J].Evidence-Based Complementary and Alternative Medicine,2014,24(05):1-10.
[2]Li Li,Guo Donghui,Wang Yiying,et al.Progress and clinical significance of the pathological study of endometrial carcinoma[J].Practical Obstetrics and Gynecology Journal,2015,31(11):806-808.[李丽,郭东辉,王轶英,等.子宫内膜癌病理研究进展及临床意义[J].实用妇产科杂志,2015,31(11):806-808.]
[3]Banno K,Yanokura M,Iida M,et al.Carcinogenic mechanisms of endometrial cancer:Involvement of genetics and epigenetics[J].Journal of Obstetrics and Gynaecology Research,2014,40(8):1957-1967.
[4]Hrzenjak A,Dieber-Rotheneder M,Moinfar F,et al.Molecular mechanisms of endometrial stromal sarcoma and undifferentiated endometrial sarcoma as premises for new therapeutic strategies[J].Cancer Letters,2014,354(1):21-27.
[5]Lou Xueling,Zhou Meiling,Zhang Zhanxin,et al.The expressions of estrogen receptor,progesterone receptor,C-erb B-2 and Ki-67 in endometrial carcinoma and their correlation with clinical pathology[J].Chinese Journal of Gynecology and Obstetrics,2014,30(07):557-560.[娄雪玲,周梅玲,张占薪,等.雌激素受体、孕激素受体、C-erb B-2和Ki-67在子宫内膜癌中的表达及其与临床病理相关性[J].中国实用妇科与产科杂志,2014,30(07):557-560.]
[6]Ning Jing,Luo Na,Zheng Dongmei.The correlation between ER,PR and Ki-67 expression and the occurrence of endometrial cancer[J].Journal of Hunan Normal University(Medical Science),2017,14(03):138-140.[宁静,罗娜,郑冬梅.ER、PR、Ki-67表达与子宫内膜癌发生的相关性研究[J].湖南师范大学学报(医学版),2017,14(03):138-140.]
[7]Zhao Yiqi,Deng Zewen,Qie Mingrong,et al.226 cases of advanced endometrial carcinoma,their clinicopathological characteristics,treatment and prognosis related factors[J].Journal of Practical Obstetrics and Gynecology,2017,33(07):506-511.[赵祎琪,邓泽文,郄明蓉,等.226例晚期子宫内膜癌的临床病理特征及治疗与其预后的相关因素分析[J].实用妇产科杂志,2017,33(07):506-511.]
[8]Li Xiaomao,Ye Huixia,Yang Xiaohui,et al.Clinicopathological analysis of endometrial carcinoma over 60 years old[J].Modern Obstetrics and Gynecology,2015,24(05):354-356.[李小毛,叶辉霞,杨晓辉,等.60岁以上子宫内膜癌临床病理分析[J].现代妇产科进展,2015,24(05):354-356.]
[9]Lv Qiaoying,Zhao Hongbo,Chen Xiaojun.Progress in the immunomodulatory mechanism of endometrial carcinoma microenvironment[J].Journal of Fudan(Medical Edition),2016,43(04):475-478.[吕巧英,赵洪波,陈晓军.子宫内膜癌肿瘤微环境的免疫调控机制进展[J].复旦学报(医学版),2016,43(04):475-478.]
[10]Wu Yadong,Wang Lina,Hu Yuhong.Advances in the molecular mechanism of endometrial cancer[J].Medical Review,2015,21(17):3135-3137.[吴雅冬,王丽娜,胡玉红.子宫内膜癌相关分子机制的研究进展[J].医学综述,2015,21(17):3135-3137.]
[11]Yang Yuan,Wang Jianliu,Wei Lihui.Notch signaling pathway and the relationship between endometriosis and endometrial cancer[J].Chinese Journal of Obstetrics and Gynecology,2015,50(5):391-393.[杨媛,王建六,魏丽惠.Notch信号通路与子宫内膜癌关系的研究进展[J].中华妇产科杂志,2015,50(5):391-393.]
[12]Zhu Minghui,Chen Ye,Wang Channi.C-erb B-2,Ki-67,ER and PR expression are correlated with the clinical pathology of endometrial carcinoma[J].Journal of Mudanjiang Medical University,2017,38(01):16-18.[朱明慧,陈烨,王婵妮.Cerb B-2、Ki-67、ER及PR表达与子宫内膜癌的临床病理相关性[J].牡丹江医学院学报,2017,38(01):16-18.]
[13]Yuan DZ,Ding XL,Yu HL,et al.Progesterone-induced Cyclin G1inhibits the proliferation of endometrial epithelial cell and its possible molecular mechanism[J].Hormone and Metabolic Research,2014,46(11):761-767.
[14]Lin C,Chen P,Wang C,et al.Antitumor effects and biological mechanism of action of the aqueous extract of the camptotheca acuminata fruit in human endometrial carcinoma cells[J].Evidence-Based Complementary and Alternative Medicine,2014,24(05):1-10.