三阴型乳腺癌患者癌组织及血清转化生长因子β_1检测的临床意义
摘要
目的探讨转化生长因子β_1(TGF-β_1)在三阴型乳腺癌发生、发展中的作用。方法选择三阴型乳腺癌患者80例、非三阴型乳腺癌患者40例,采用ELISA法检测血清TGF-β_1水平,采用免疫组化法检测乳腺癌组织TGF-β_1表达,分析TGF-β_1表达与三阴型乳腺癌患者临床病理参数及预后的关系。结果三阴型及非三阴型乳腺癌患者血清TGF-β_1水平分别为(108.43±10.55)、(84.09±11.52)ng/L,二者比较P<0.05。三阴型乳腺癌患者中TGF-β_1高表达42例、低表达38例,TGF-β_1高表达者较低表达者腋下淋巴结转移率高、组织学分级程度低(P均<0.01),二者年龄、绝经状态、肿瘤直径、乳腺癌家族史比较差异均无统计学意义(P均>0.05);TGF-β_1高表达者5年无瘤生存率为54.76%(23/42)、低表达者为71.05%(27/38),二者比较P<0.05。结论三阴型乳腺癌患者TGF-β_1高表达,TGF-β_1的表达变化与肿瘤组织学分级及腋下淋巴结转移相关,并影响患者预后。
引文
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[14]Bao J,Wu ZS,Qi Y,et al.Expression of TGF-beta1 and the mechanism of invasiveness and metastasis induced by TGF-beta1 in breast cancer[J].Zhonghua Zhong Liu Za Zhi,2009,31(9):679-828.
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[17]高炳玉,刘玉,夏立平,等.三阴性乳腺癌和非三阴性乳腺癌临床特征及预后的比较分析[J].肿瘤防治研究,2013,40(8):786-788.
[2]Rosman DS,Phukan S,Huang CC,et al.TGFBR1*6A enhances the migration and invasion of MCF-7 breast cancer cells through Rho A activation[J].Cancer Res,2008,68(5):1319-1328
[3]Yang YN,Pan XC,Lei WW,et al.Regulation of transforming growth factor-β1-induced apoptosis and epithelial-to-mesenchymal transition by protein kinase a and signatl Transducers and activators of Ttranscription 3[J].Cancer Res,2006,66(17):8617-8624
[4]Bierie B,Moses HL.Gain or loss of TGFbeta signaling in mammary carcinoma cells can promote metastasis[J].Cell Cycle,2009,8(20):3319-3327.
[5]Nagaraj NS,Datta PK.Targeting the transforming growth factor-beta signaling pathway in human cancer[J].Expert Opin Investig Drugs,2010,19(1):77-91.
[6]沈卫东,刘鹏飞,刘兵团,等.TGF-β1和Smad2在胃癌中的表达及意义[J].世界华人消化杂志,2012,20(14):1238-1241.
[7]Minamiya Y,Miura M,Hinai Y,et al.Transforming growth factor-β129T>C genetic polymorphism is associated with lymph node metastasis in patients with adenocarcinoma of the lung[J].Tumour Biol,2010,31(5):437-441.
[8]周瑞,丁明剑,熊斌.转化生长因子β1型受体多态性在结肠癌细胞侵袭迁移中的作用[J].中华临床医师杂志(电子版),2012,6(22):7200-7204.
[9]Bhola NE,Balko JM,Dugger TC,et al.TGF-βinhibition enhances chemotherapy action against triple-negative breast cancer[J].J Clin Invest,2013,123(3):1348-1358.
[10]Lang DS,Marwitz S,Heilenk?tter U,et al.Transforming growth factor-beta signaling leads to u PA/PAI-1 activation and metastasis:a study on human breast cancer tissues[J].Pathol Oncol Res,2014,20(3):727-732.
[11]Roberts AB,Wakefield LM.The two faces of transforming growth factor in carcinogenesis[J].PNAS,2003,100(15):8621-8623.
[12]Jakowlew SB.Transforming growth factor-βin cancer and metastasis[J].Cancer Metastasis Rev,2006,25(3):435-457.
[13]Bhola NE,Balko JM,Dugger TC,et al.TGF-βinhibition enhances chemotherapy action against triple-negative breast cancer[J].J Clin Invest,2013,123(3):1348-1358.
[14]Bao J,Wu ZS,Qi Y,et al.Expression of TGF-beta1 and the mechanism of invasiveness and metastasis induced by TGF-beta1 in breast cancer[J].Zhonghua Zhong Liu Za Zhi,2009,31(9):679-828.
[15]Xue J,Lin X,Chiu WT,et al.Sustained activation of SMAD3/SMAD4 by FOXM1 promotes TGF-β-dependent cancer metastasis[J].J Clin Invest,2014,124(2):564-579.
[16]Buck MB,Fritz P,Dippon J,et al.Prognostic significance of transforming growth factor receptorⅡin estrogen receptor-negative breast cancer patients[J].Clin Cancer Res,2004,10(2):491-498.
[17]高炳玉,刘玉,夏立平,等.三阴性乳腺癌和非三阴性乳腺癌临床特征及预后的比较分析[J].肿瘤防治研究,2013,40(8):786-788.