DEAE-琼脂糖LB膜的制备与表征
|
摘要
由于单分子膜性能独特,受到关注度越来越高,利用LB膜技术制备了二乙氨乙基(DEAE)-琼脂糖单分子层。分析其表面压(π)-面积(A)曲线,崩溃压较高,成膜过程明显。研究浓度对DEAE-琼脂糖成膜情况的影响,确定最佳成膜浓度为0.5 mg/m L。考察配基密度与表面电势的关系,其表面电势随着配基密度的增加而增大。采用原子力显微镜(AFM)和布鲁斯特角显微镜(BAM)对制备的LB膜进行了表征,得出表面膜的表面平整、紧密,厚度约为7.9 nm。DEAE-琼脂糖LB膜制备成功,为以后LB膜技术的广泛应用提供了一种新的材料。
Diethylaminoethyl( DEAE)-Agarose was studied for preparing monolayers based on the Langmuir-Blodgett( LB) film technology. A high collapse pressure and a standard film forming process were found in the surface pressure( π)-area( A) isotherm of DEAE-Agarose. The effect of DEAE-Agarose concentration on the film formation showed that 0. 5 mg / m L was the best. The surface potential increased with rise of the ligand density. DEAE-Agarose LB films were characterized with Brewster angle microscopy( BAM) and atomic force microscope( AFM) after being transferred onto silicon surface. The morphology of the film was pretty compact,well structured,and relatively smooth. The thickness was about 7. 9 nm and the roughness was about 2 nm. This result indicated that the DEAEAgarose LB film was successfully prepared and could be an novel material for the fabrication of LB films.
Diethylaminoethyl( DEAE)-Agarose was studied for preparing monolayers based on the Langmuir-Blodgett( LB) film technology. A high collapse pressure and a standard film forming process were found in the surface pressure( π)-area( A) isotherm of DEAE-Agarose. The effect of DEAE-Agarose concentration on the film formation showed that 0. 5 mg / m L was the best. The surface potential increased with rise of the ligand density. DEAE-Agarose LB films were characterized with Brewster angle microscopy( BAM) and atomic force microscope( AFM) after being transferred onto silicon surface. The morphology of the film was pretty compact,well structured,and relatively smooth. The thickness was about 7. 9 nm and the roughness was about 2 nm. This result indicated that the DEAEAgarose LB film was successfully prepared and could be an novel material for the fabrication of LB films.
引文
[1]张俊苓,杨芳,郑文杰,等.自组装单分子膜及其表征方法[J].化学进展,2005,17(2):203-208.
[2]张一芳.基于纳米材料的生物传感技术在食品安全中的应用[D].长沙:湖南大学,2011.
[3]马翠翠.基于紫外光降解自组装单分子层的纸芯片研制及其应用研究[D].杭州:浙江大学,2013.
[4]齐平.卟啉LB膜的制备和性能研究[D].长春:吉林大学,2013.
[5]ZOU L,LI Y,CAO S,et al.Gold nanoparticles/polyaniline Langmuir–Blodgett Film modified glassy carbon electrode as voltammetric sensor for detection of epinephrine and uric acid[J].Talanta,2013,117:333-337.
[6]LEE J H,KANG W S,NAJEEB C K,et al.A hydrogen gas sensor using single-walled carbon nanotube Langmuir Blodgett films decorated with palladium nanoparticles[J].Sensors and Actuators B:Chemical,2013,188:169-175.
[7]白桦.运用LB技术构筑有机半导体超薄膜[D].甘肃:兰州大学,2009.
[8]GURFIDAN L,OZMEN M,ATACAN K,et al.Preparation and characterization of calix
[6]arene Langmuir–Blodgett thin film[J].Thin Solid Films,2012,520(19):6238-6242.
[9]PARK Y H,JANGA J I,KIM K H,et al.Preparation and characterization of electroconductive poly(thiophene-co-pyrrolylundecanoic acid)Langmuir Blodgett films[J].Thin Solid Films,2005,489(1-2):215-220.
[10]CHEN Q,XU S,LIANG X,et al.Network structure of collagen layers absorbed on LB film[J].Journal of Colloid and Interface Science,2007,316(1):1-9.
[11]汪建中,李艳如,龚华锐.平菇菌粗多糖的抗氧化活性研究[J].生物学杂志,2014,31(1):15-19.
[12]安晓娟,冯琳,宋红平,等.淫羊藿多糖的分离纯化及结构初步分析[J].生物学杂志,2012,29(3):39-41.
[13]QING W X,WANG Y,WANG Y Y,et al.The modified nanocrystalline cellulose for hydrophobic drug delivery[J].Applied Surface Science,2016,366:404-409.
[14]KONG B J,KIM A,PARK S N,et al.Properties and in vitro drug release of hyaluronic acid-hydroxyethyl cellulose hydrogels for transdermal delivery of isoliquiritigenin[J].Carbohydrate Polymers,2016,147:473-481.
[15]TANG Y D,LIU J M,CHEN Y.Agarose multi-wells for tumour spheroid formation and anti-cancer drug test[J].Microelectronic Engineering,2016,158:41-45.
[16]李龙.龙须菜琼脂糖的提取技术研究[D].福州:福建农林大学,2013.
[17]吴钢.低熔点琼脂糖的改性与制备技术研究[D].福州:福建农林大学,2014.
[18]苏永昌,李龙,刘淑集,等.响应面法优化琼脂糖的DEAE-纤维素法提取工艺[J].食品工业科技,2014,35(24):234-238.
[19]IFUKU S,NAKAI S,KAMITAKAHARA H,et al.Preparation and characterization of monolayer and multilayer Langmuir-Blodgett films of a series of 6-O-alkylcelluloses[J].Biomacromolecules,2005,6(4):2067-2073.
[20]杨小雁.离子交换配基密度对蛋白质吸附行为的影响[D].泰安:山东农业大学,2015.
[21]HABIBI Y,FOULON L,AGUIé-BéGHIN V,et al.LangmuirBlodgett films of cellulose nanocrystals:preparation and characterization[J].Journal of Colloid and Interface Science,2007,316(2):388-397.
[2]张一芳.基于纳米材料的生物传感技术在食品安全中的应用[D].长沙:湖南大学,2011.
[3]马翠翠.基于紫外光降解自组装单分子层的纸芯片研制及其应用研究[D].杭州:浙江大学,2013.
[4]齐平.卟啉LB膜的制备和性能研究[D].长春:吉林大学,2013.
[5]ZOU L,LI Y,CAO S,et al.Gold nanoparticles/polyaniline Langmuir–Blodgett Film modified glassy carbon electrode as voltammetric sensor for detection of epinephrine and uric acid[J].Talanta,2013,117:333-337.
[6]LEE J H,KANG W S,NAJEEB C K,et al.A hydrogen gas sensor using single-walled carbon nanotube Langmuir Blodgett films decorated with palladium nanoparticles[J].Sensors and Actuators B:Chemical,2013,188:169-175.
[7]白桦.运用LB技术构筑有机半导体超薄膜[D].甘肃:兰州大学,2009.
[8]GURFIDAN L,OZMEN M,ATACAN K,et al.Preparation and characterization of calix
[6]arene Langmuir–Blodgett thin film[J].Thin Solid Films,2012,520(19):6238-6242.
[9]PARK Y H,JANGA J I,KIM K H,et al.Preparation and characterization of electroconductive poly(thiophene-co-pyrrolylundecanoic acid)Langmuir Blodgett films[J].Thin Solid Films,2005,489(1-2):215-220.
[10]CHEN Q,XU S,LIANG X,et al.Network structure of collagen layers absorbed on LB film[J].Journal of Colloid and Interface Science,2007,316(1):1-9.
[11]汪建中,李艳如,龚华锐.平菇菌粗多糖的抗氧化活性研究[J].生物学杂志,2014,31(1):15-19.
[12]安晓娟,冯琳,宋红平,等.淫羊藿多糖的分离纯化及结构初步分析[J].生物学杂志,2012,29(3):39-41.
[13]QING W X,WANG Y,WANG Y Y,et al.The modified nanocrystalline cellulose for hydrophobic drug delivery[J].Applied Surface Science,2016,366:404-409.
[14]KONG B J,KIM A,PARK S N,et al.Properties and in vitro drug release of hyaluronic acid-hydroxyethyl cellulose hydrogels for transdermal delivery of isoliquiritigenin[J].Carbohydrate Polymers,2016,147:473-481.
[15]TANG Y D,LIU J M,CHEN Y.Agarose multi-wells for tumour spheroid formation and anti-cancer drug test[J].Microelectronic Engineering,2016,158:41-45.
[16]李龙.龙须菜琼脂糖的提取技术研究[D].福州:福建农林大学,2013.
[17]吴钢.低熔点琼脂糖的改性与制备技术研究[D].福州:福建农林大学,2014.
[18]苏永昌,李龙,刘淑集,等.响应面法优化琼脂糖的DEAE-纤维素法提取工艺[J].食品工业科技,2014,35(24):234-238.
[19]IFUKU S,NAKAI S,KAMITAKAHARA H,et al.Preparation and characterization of monolayer and multilayer Langmuir-Blodgett films of a series of 6-O-alkylcelluloses[J].Biomacromolecules,2005,6(4):2067-2073.
[20]杨小雁.离子交换配基密度对蛋白质吸附行为的影响[D].泰安:山东农业大学,2015.
[21]HABIBI Y,FOULON L,AGUIé-BéGHIN V,et al.LangmuirBlodgett films of cellulose nanocrystals:preparation and characterization[J].Journal of Colloid and Interface Science,2007,316(2):388-397.