经济海藻羊栖菜(Sargassum fusiforme)受精卵生物活性物质及矿质元素成分分析
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摘要
为了综合评价经济海藻羊栖菜(Sargassum fusiforme)受精卵的营养价值及开发利用前景,本文检测了羊栖菜受精卵碳水化合物、膳食纤维、氨基酸、脂肪酸等生物活性物质与矿质元素成分,并与文献报道的孢子体相应成分进行了比较。结果发现:羊栖菜受精卵富含碳水化合物、蛋白质、氨基酸及膳食纤维,其含量分别为(20.4±0.07)、(22.7±0.03)、(16.7±0.04)和(39.2±0.03)g/100g DW;谷氨酸、维生素E和β-类胡萝卜素含量尤其高,分别达到(9.82±0.05)g/100g DW、(11.8±0.01)mg/100g DW和(3.0×103±7.07)μg/100gDW;而脂肪含量偏低,只有1.03%。矿质元素中微量元素铁、钙和镁的含量较高,分别为(4.54×103±49.50)、(1.83×103±7.78)和(1.40×103±28.28)mg/100g DW。通过与文献报道的羊栖菜孢子体生化成分相比较,羊栖菜受精卵中蛋白质、氨基酸、β-类胡萝卜素、钾、钠、磷、铁、钙、锌、锰及铜元素的含量均高于羊栖菜孢子体。说明羊栖菜受精卵具有更高的天然产物提取、开发和利用价值。
To comprehensively evaluate the nutritional values and development prospects of fertilized eggs of Sargassum fusiforme, the mineral elements, bioactive substances such as carbohydrates, dietary fibers, amino acids and fatty acids in the fertilized eggs were determined and compared with the corresponding components in the sporophytes based on literatures. The results showed that the fertilized eggs of S. fusiforme were rich in carbohydrates, proteins, amino acids and dietary fibers, and the corresponding contents were(20.4±0.07),(22.7±0.03),(16.7±0.04) and(39.2±0.03)g/100 g DW, respectively. In addition, the contents of glutamic acid, vitamin E and β-carotene were particularly high, reaching(9.82±0.05)g/100 g DW,(11.8±0.01)mg/100 g DW and(3.0×103±7.07)μg/100 g DW, respectively. However, the content of fat was low, only 1.03%. For the mineral elements, the contents of trace elements iron, calcium and magnesium were high,reaching(4.54×103±49.50),(1.83×103±7.78) and(1.40×103±28.28)mg/100 g DW, respectively. In conclusion, compared with the biochemical components in sporophytes of S. fusiformis reported in the literatures, the fertilized eggs contain higher contents of proteins, amino acids, β-carotene, potassium, sodium, phosphorus, iron, calcium, zinc, manganese and copper. It indicates that the fertilized eggs of S. fusiforme is better than the sporophytes in extraction, development and utilization of the natural products.
To comprehensively evaluate the nutritional values and development prospects of fertilized eggs of Sargassum fusiforme, the mineral elements, bioactive substances such as carbohydrates, dietary fibers, amino acids and fatty acids in the fertilized eggs were determined and compared with the corresponding components in the sporophytes based on literatures. The results showed that the fertilized eggs of S. fusiforme were rich in carbohydrates, proteins, amino acids and dietary fibers, and the corresponding contents were(20.4±0.07),(22.7±0.03),(16.7±0.04) and(39.2±0.03)g/100 g DW, respectively. In addition, the contents of glutamic acid, vitamin E and β-carotene were particularly high, reaching(9.82±0.05)g/100 g DW,(11.8±0.01)mg/100 g DW and(3.0×103±7.07)μg/100 g DW, respectively. However, the content of fat was low, only 1.03%. For the mineral elements, the contents of trace elements iron, calcium and magnesium were high,reaching(4.54×103±49.50),(1.83×103±7.78) and(1.40×103±28.28)mg/100 g DW, respectively. In conclusion, compared with the biochemical components in sporophytes of S. fusiformis reported in the literatures, the fertilized eggs contain higher contents of proteins, amino acids, β-carotene, potassium, sodium, phosphorus, iron, calcium, zinc, manganese and copper. It indicates that the fertilized eggs of S. fusiforme is better than the sporophytes in extraction, development and utilization of the natural products.
引文
王百龙,孙稚颖,周凤琴,2015.羊栖菜成分以及药理研究进展.山东中医杂志,34(9):722-725
尤瑜敏,许时婴,2002.羊栖菜中褐藻糖胶的提取工艺.无锡轻工大学学报,21(3):233-238
毛文君,管华诗,1995.12种海藻硒含量及其分布特点的研究.中国海洋药物杂志,14(1):27-30
朱金霞,周文生,郭生虎,2009.植物中微量元素硒的研究进展.安徽农业科学,37(13):5844-5845
刘红英,薛长湖,江艳华等,2003.我国部分经济海产食品中砷、铅、镉、汞含量的测定与分析.中国食品学报,(S1):175-179
阮积惠,徐礼根,2001.羊栖菜Sargassum fusiforme Setch繁殖与发育生物学的初步研究.浙江大学学报(理学版),28(3):315-320
孙建璋,方家仲,朱植丰等,1996.羊栖菜Sargassum fusiforme(Harve)Setch繁殖生物学的初步研究.浙江水产学报,15(4):243-249
李丽,陶平,安风飞,2002.羊栖菜(Sargassum fusiforme)的营养组成分析.中国公共卫生管理,18(6):548-550
李八方,2003.羊栖菜功能多糖化学及其生物活性研究.青岛:中国科学院研究生院(海洋研究所)博士学位论文
李卫华,王雅珍,刘玉海,2006.高效液相色谱-电感耦合等离子体质谱检测海藻中的砷.陕西师范大学学报(自然科学版),34(4):65-68
李雅静,范家兴,程柳坤等,2017.两个品系羊栖菜褐藻胶与褐藻糖胶的联合提取及理化性质测定.食品工业科技,38(6):90-95,100
陈帆,陈世理,2002.海洋药用羊栖菜中总砷含量的分析.温州师范学院学报(自然科学版),23(6):37-39
陈群,许宁,2005.羊栖菜活性成分和药理作用研究进展.中国药业,14(6):95-96
陈绍瑗,陈耀祖,莫卫民,1998.海洋药物研究(Ⅱ)--羊栖菜中蛋白质和氨基酸分析.浙江工业大学学报,26(1):45-48
陈耀祖,潘远江,莫卫民,1996.东海药用海藻化学成份分析研究(I)羊栖菜中微量元素分析.浙江大学学报(自然科学版),30(4):471-473
国家药典委员会,2015.中华人民共和国药典2015年版一部.北京:中国医药科技出版社
周青,1986.植物中氨基酸的生理作用.生物学通报,(8):7-9
郑立,严小军,孙建等,2005.日本真海带对硒的积累和生物转化.海洋学报,27(5):119-125
赵进沛,刘卫,陈连庆,2000.微量元素在保健食品研制开发中的应用.广东微量元素科学,7(7):11-14
赵艳芳,尚德荣,宁劲松等,2013.羊栖菜中微量金属元素的亚细胞分区分布.渔业科学进展,34(3):118-123
胡敏,于凤文,洪机剑等,2017.5种生物质基本性质分析.西北林学院学报,32(3):52-55
骆其君,林少珍,2013.羊栖菜--海岛洞头的金名片.北京:海洋出版社
夏静芬,汪财生,钱国英,2009.羊栖菜和海带脂肪酸组成的比较分析.食品研究与开发,30(12):5-8
高杨,曹煊,余晶晶等,2009.高效液相色谱-电感耦合等离子体质谱联用技术测定干海产品中砷化学形态.分析化学,37(12):1738-1742
曾呈奎,陆保仁,2000.中国海藻志第三卷褐藻门第二册墨角藻目.北京:科学出版社
温州市海洋与渔业局,2015.2014年温州市海洋环境公报.http://xxgk.wenzhou.gov.cn
温州市海洋与渔业局,2016.2015年温州市海洋环境公报.https://mp.weixin.qq.com/s?__bi z=MzA4ODY2NzYyMQ%3D%3D&idx=1&mid=401850591&sn=e0ad7362e3b756aef72a4d2baf388b20
温州市海洋与渔业局,2017.2016年温州市海洋环境公报.http://wzhy.wenzhou.gov.cn/art/2017/3/8/art_1252564_5868666.html
温州市海洋与渔业局,2018.2017年温州市海洋环境公报.http://xxgk.wenzhou.gov.cn
戴志远,洪泳平,张燕平等,2002.羊栖菜的营养成分分析与评价.水产学报,26(4):382-384
Choi E Y,Hwang H J,Kim I H et al,2009.Protective effects of a polysaccharide from Hizikia fusiformis against ethanol toxicity in rats.Food and Chemical Toxicology,47(1):134-139
Davis T A,Volesky B,Mucci A,2003.A review of the biochemistry of heavy metal biosorption by brown algae.Water Research,37(18):4311-4330
Hwang H J,Kim I H,Nam T J,2008.Effect of a glycoprotein from Hizikia fusiformis on acetaminophen-induced liver injury.Food and Chemical Toxicology,46(11):3475-3481
Laparra J M,Vélez D,Montoro R et al,2004.Bioaccessibility of inorganic arsenic species in raw and cooked Hizikia fusiforme seaweed.Applied Organometallic Chemistry,18(12):662-669
Li B,Wei X J,Sun J L et al,2006.Structural investigation of a fucoidan containing a fucose-free core from the brown seaweed,Hizikia fusiforme.Carbohydrate Research,341(9):1135-1146
Ma Z L,Lin L D,Wu M J et al,2018.Total and inorganic arsenic contents in seaweeds:absorption,accumulation,transformation and toxicity.Aquaculture,497:49-55
Pang S J,Chen L T,Zhuang D G et al,2005.Cultivation of the brown alga Hizikia fusiformis(Harvey)Okamura:enhanced seedling production in tumbled culture.Aquaculture245(1-4):321-329
Pang S J,Gao S Q,Sun J Z,2006.Cultivation of the brown alga Hizikia fusiformis(Harvey)Okamura:controlled fertilization and early development of seedlings in raceway tanks in ambient light and temperature.Journal of Applied Phycology,18(6):723-731
Pang S J,Shan T F,Zhang Z H et al,2008.Cultivation of the intertidal brown alga Hizikia fusiformis(Harvey)Okamura:mass production of zygote-derived seedlings under commercial cultivation conditions,a case study experience.Aquaculture Research,39(13):1408-1415
Rose M,Lewis J,Langford N et al,2007.Arsenic in seaweed-forms,concentration and dietary exposure.Food and Chemical Toxicology,45(7):1263-1267
Tamilselvan N,Saurav K,Kannabiran K,2012.Biosorption of Cr(VI),Cr(III),Pb(II)and Cd(II)from aqueous solutions by Sargassum wightii and Caulerpa racemosa algal biomass.Journal of Ocean University of China,11(1):52-58
Vilar V J P,Botelho C M S,Boaventura R A R,2007.Copper desorption from Gelidium algal biomass.Water Research,41(7):1569-1579
Zhu T,Heo H J,Row K H,2010.Optimization of crude polysaccharides extraction from Hizikia fusiformis using response surface methodology.Carbohydrate Polymers,82(1):106-110
尤瑜敏,许时婴,2002.羊栖菜中褐藻糖胶的提取工艺.无锡轻工大学学报,21(3):233-238
毛文君,管华诗,1995.12种海藻硒含量及其分布特点的研究.中国海洋药物杂志,14(1):27-30
朱金霞,周文生,郭生虎,2009.植物中微量元素硒的研究进展.安徽农业科学,37(13):5844-5845
刘红英,薛长湖,江艳华等,2003.我国部分经济海产食品中砷、铅、镉、汞含量的测定与分析.中国食品学报,(S1):175-179
阮积惠,徐礼根,2001.羊栖菜Sargassum fusiforme Setch繁殖与发育生物学的初步研究.浙江大学学报(理学版),28(3):315-320
孙建璋,方家仲,朱植丰等,1996.羊栖菜Sargassum fusiforme(Harve)Setch繁殖生物学的初步研究.浙江水产学报,15(4):243-249
李丽,陶平,安风飞,2002.羊栖菜(Sargassum fusiforme)的营养组成分析.中国公共卫生管理,18(6):548-550
李八方,2003.羊栖菜功能多糖化学及其生物活性研究.青岛:中国科学院研究生院(海洋研究所)博士学位论文
李卫华,王雅珍,刘玉海,2006.高效液相色谱-电感耦合等离子体质谱检测海藻中的砷.陕西师范大学学报(自然科学版),34(4):65-68
李雅静,范家兴,程柳坤等,2017.两个品系羊栖菜褐藻胶与褐藻糖胶的联合提取及理化性质测定.食品工业科技,38(6):90-95,100
陈帆,陈世理,2002.海洋药用羊栖菜中总砷含量的分析.温州师范学院学报(自然科学版),23(6):37-39
陈群,许宁,2005.羊栖菜活性成分和药理作用研究进展.中国药业,14(6):95-96
陈绍瑗,陈耀祖,莫卫民,1998.海洋药物研究(Ⅱ)--羊栖菜中蛋白质和氨基酸分析.浙江工业大学学报,26(1):45-48
陈耀祖,潘远江,莫卫民,1996.东海药用海藻化学成份分析研究(I)羊栖菜中微量元素分析.浙江大学学报(自然科学版),30(4):471-473
国家药典委员会,2015.中华人民共和国药典2015年版一部.北京:中国医药科技出版社
周青,1986.植物中氨基酸的生理作用.生物学通报,(8):7-9
郑立,严小军,孙建等,2005.日本真海带对硒的积累和生物转化.海洋学报,27(5):119-125
赵进沛,刘卫,陈连庆,2000.微量元素在保健食品研制开发中的应用.广东微量元素科学,7(7):11-14
赵艳芳,尚德荣,宁劲松等,2013.羊栖菜中微量金属元素的亚细胞分区分布.渔业科学进展,34(3):118-123
胡敏,于凤文,洪机剑等,2017.5种生物质基本性质分析.西北林学院学报,32(3):52-55
骆其君,林少珍,2013.羊栖菜--海岛洞头的金名片.北京:海洋出版社
夏静芬,汪财生,钱国英,2009.羊栖菜和海带脂肪酸组成的比较分析.食品研究与开发,30(12):5-8
高杨,曹煊,余晶晶等,2009.高效液相色谱-电感耦合等离子体质谱联用技术测定干海产品中砷化学形态.分析化学,37(12):1738-1742
曾呈奎,陆保仁,2000.中国海藻志第三卷褐藻门第二册墨角藻目.北京:科学出版社
温州市海洋与渔业局,2015.2014年温州市海洋环境公报.http://xxgk.wenzhou.gov.cn
温州市海洋与渔业局,2016.2015年温州市海洋环境公报.https://mp.weixin.qq.com/s?__bi z=MzA4ODY2NzYyMQ%3D%3D&idx=1&mid=401850591&sn=e0ad7362e3b756aef72a4d2baf388b20
温州市海洋与渔业局,2017.2016年温州市海洋环境公报.http://wzhy.wenzhou.gov.cn/art/2017/3/8/art_1252564_5868666.html
温州市海洋与渔业局,2018.2017年温州市海洋环境公报.http://xxgk.wenzhou.gov.cn
戴志远,洪泳平,张燕平等,2002.羊栖菜的营养成分分析与评价.水产学报,26(4):382-384
Choi E Y,Hwang H J,Kim I H et al,2009.Protective effects of a polysaccharide from Hizikia fusiformis against ethanol toxicity in rats.Food and Chemical Toxicology,47(1):134-139
Davis T A,Volesky B,Mucci A,2003.A review of the biochemistry of heavy metal biosorption by brown algae.Water Research,37(18):4311-4330
Hwang H J,Kim I H,Nam T J,2008.Effect of a glycoprotein from Hizikia fusiformis on acetaminophen-induced liver injury.Food and Chemical Toxicology,46(11):3475-3481
Laparra J M,Vélez D,Montoro R et al,2004.Bioaccessibility of inorganic arsenic species in raw and cooked Hizikia fusiforme seaweed.Applied Organometallic Chemistry,18(12):662-669
Li B,Wei X J,Sun J L et al,2006.Structural investigation of a fucoidan containing a fucose-free core from the brown seaweed,Hizikia fusiforme.Carbohydrate Research,341(9):1135-1146
Ma Z L,Lin L D,Wu M J et al,2018.Total and inorganic arsenic contents in seaweeds:absorption,accumulation,transformation and toxicity.Aquaculture,497:49-55
Pang S J,Chen L T,Zhuang D G et al,2005.Cultivation of the brown alga Hizikia fusiformis(Harvey)Okamura:enhanced seedling production in tumbled culture.Aquaculture245(1-4):321-329
Pang S J,Gao S Q,Sun J Z,2006.Cultivation of the brown alga Hizikia fusiformis(Harvey)Okamura:controlled fertilization and early development of seedlings in raceway tanks in ambient light and temperature.Journal of Applied Phycology,18(6):723-731
Pang S J,Shan T F,Zhang Z H et al,2008.Cultivation of the intertidal brown alga Hizikia fusiformis(Harvey)Okamura:mass production of zygote-derived seedlings under commercial cultivation conditions,a case study experience.Aquaculture Research,39(13):1408-1415
Rose M,Lewis J,Langford N et al,2007.Arsenic in seaweed-forms,concentration and dietary exposure.Food and Chemical Toxicology,45(7):1263-1267
Tamilselvan N,Saurav K,Kannabiran K,2012.Biosorption of Cr(VI),Cr(III),Pb(II)and Cd(II)from aqueous solutions by Sargassum wightii and Caulerpa racemosa algal biomass.Journal of Ocean University of China,11(1):52-58
Vilar V J P,Botelho C M S,Boaventura R A R,2007.Copper desorption from Gelidium algal biomass.Water Research,41(7):1569-1579
Zhu T,Heo H J,Row K H,2010.Optimization of crude polysaccharides extraction from Hizikia fusiformis using response surface methodology.Carbohydrate Polymers,82(1):106-110