MicroRNA相关基因Dicer rs1057035多态性与脑胶质瘤的相关性研究
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摘要
目的探讨陕西地区汉族人群中,Dicer基因rs1057035多态性与脑胶质瘤的相关性。方法 200例陕西地区脑胶质瘤患者作为病例组,随机选择同期在我科住院治疗的非肿瘤疾病患者184例作为对照组,收集所有入住患者相关临床资料,并采用聚合酶链反应-限制性内切酶分析(PCR-RFLP)法检测Dicer基因rs1057035单核苷酸多态性。结果病例组患者Dicer基因rs1057035位点等位基因C分布频率明显低于对照组(χ~2=5.137,P=0.023)。分层分析,携带突变等位基因C可降低酗酒者,无肿瘤性疾病家族史者以及Ⅲ型、Ⅳ型胶质瘤患者发病风险(OR=0.253,95%CI 0.067～0.949,P=0.036;OR=0.561,95%CI 0.339～0.930,P=0.024;OR=0.525,95%CI 0.284～0.969,P=0.037)。结论 Dicer基因rs1057035多态性可能与脑胶质瘤遗传易感性相关。
Objective To investigate the association between the Dicer gene rs1057035 polymorphism and glioma in the Han Chinese population in Shaanxi Province,China. Methods A total of 200 patients with glioma in Shaanxi Province were enrolled as case group,and 184 patients without tumor diseases who were hospitalized and treated in our department during the same period of time were enrolled as control group. Their clinical data were collected,and polymerase chain reaction-restriction fragment length polymorphism was used to analyze the single nucleotide polymorphism of Dicer gene rs1057035. Results The case group had a significantly lower frequency of C allele of Dicer gene rs1057035 than the control group( χ~2= 5. 137,P = 0. 023). The stratified analysis showed that Dicer rs1057035 C allele significantly reduced the risk of glioma in alcoholics( odds ratio [OR]= 0. 253,95% confidence interval [CI]:0. 067-0. 949,P = 0. 036),patients without a family history of tumor disease( OR = 0. 561,95% CI: 0. 339-0. 930,P = 0. 024),or patients with type III/IV glioma( OR = 0. 525,95% CI: 0. 284-0. 969,P = 0. 037). Conclusions The Dicer rs1057035 polymorphism may be associated with the genetic susceptibility to glioma.
Objective To investigate the association between the Dicer gene rs1057035 polymorphism and glioma in the Han Chinese population in Shaanxi Province,China. Methods A total of 200 patients with glioma in Shaanxi Province were enrolled as case group,and 184 patients without tumor diseases who were hospitalized and treated in our department during the same period of time were enrolled as control group. Their clinical data were collected,and polymerase chain reaction-restriction fragment length polymorphism was used to analyze the single nucleotide polymorphism of Dicer gene rs1057035. Results The case group had a significantly lower frequency of C allele of Dicer gene rs1057035 than the control group( χ~2= 5. 137,P = 0. 023). The stratified analysis showed that Dicer rs1057035 C allele significantly reduced the risk of glioma in alcoholics( odds ratio [OR]= 0. 253,95% confidence interval [CI]:0. 067-0. 949,P = 0. 036),patients without a family history of tumor disease( OR = 0. 561,95% CI: 0. 339-0. 930,P = 0. 024),or patients with type III/IV glioma( OR = 0. 525,95% CI: 0. 284-0. 969,P = 0. 037). Conclusions The Dicer rs1057035 polymorphism may be associated with the genetic susceptibility to glioma.
引文
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[2]Tu Y,Gao X,Li G,et al.Micro RNA-218 inhibits glioma invasion,migration,proliferation,and cancer stem-like cell self-renewal by targeting the polycomb group gene Bmi 1[J].Cancer Res,2013,73(19):6046-6055.
[3]Yang TQ,Lu XJ,Wu TF,et al.Micro RNA‐16 inhibits glioma cell growth and invasion through suppression of BCL 2and the nuclear factor-κB 1/MMP 9 signaling pathway[J].Cancer Sci,2014,105(3):265-271.
[4]Palumbo S,Miracco C,Pirtoli L,et al.Emerging roles of micro RNA in modulating cell-death processes in malignant glioma[J].J Cell Physiol,2014,229(3):277-286.
[5]Heravi-Moussavi A,Anglesio MS,Cheng SW,et al.Recurrent somatic DICER 1 mutations in nonepithelial ovarian cancers[J].N Engl J Med,2012,366(3):234-242.
[6]Anglesio MS,Wang Y,Yang W,et al.Cancer-associated somatic DICER 1 hotspot mutations cause defective mi RNA processing and reverse-strand expression bias to predominantly mature 3 p strands through loss of 5 p strand cleavage[J].J Pathol,2013,229(3):400-409.
[7]Yu YY,Kuang D,Yin XX.Association between the DICER rs 1 0 5 7 0 3 5 polymorphism and cancer risk:evidence from a meta-analysis of 1,2675 individuals.[J].Asian Pac J Cancer Prev,2015,16(1):119-124.
[8]付胜伟.微小分子RNA在胶质瘤中的研究进展[J].国际神经病学神经外科学杂志,2012,39(4):373-3 7 6.
[9]刘于海,牛朝诗.Micro RNA与胶质母细胞瘤的相关性研究[J].国际神经病学神经外科学杂志,2009,36(5):412-416.
[10]Rah H,Jeon YJ,Lee BE,et al.Association of polymorphisms in micro RNA machinery genes(DROSHA,DICER 1,RAN,and XPO 5)with risk of idiopathic primary ovarian insufficiency in Korean women[J].Menopause,2013,20(10):1067-1073.
[11]Kurzynskakokorniak A,Koralewska N,Pokornowska M,et al.The many faces of Dicer:the complexity of the mechanisms regulating Dicer gene expression and enzyme activities[J].Nucleic Acids Res,2015,43(9):4365-4380.
[12]Chiosea S,Jelezcova E,Chandran U,et al.Overexpression of Dicer in precursor lesions of lung adenocarcinoma[J].Cancer Res,2007,67(5):2345-2350.
[13]Jafarnejad SM,Ardekani GS,Ghaffari M,et al.Sox4 Mediated Dicer Expression is Critical for Suppression of Melanoma Cell Invasion[J].Oncogene,2013,32(17):213 1-2139.
[14]Michael S,Marina S,Daniel S,et al.Comparative microarray analysis of micro RNA expression profiles in primary cutaneous malignant melanoma,cutaneous malignant melanoma metastases,and benign melanocytic nevi[J].Cell Tissue Res,2013,351(1):85-98.