人类ARGONAUTE和DICER基因家族在结肠癌中的表达分析
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摘要
目的研究分析人类ARGONAUTE和DICER基因家族在结肠癌中的表达意义。方法通过实时荧光定量聚合酶链式反应(PCR)测定人类ARGONAUTE和DICER基因家族在结肠癌组织和癌旁组织中的表达。结果与癌旁组织比较,结肠癌组织中AGO2表达明显上调,其他AGO基因表达均明显下调,DICER家族表达均明显下调,差异均有统计学意义(P<0.05)。结论 ARGONAUTE和DICER家族的异常表达表明其可能参与结肠癌的发生、发展。
Objective To analyze the expression of human ARGONAUTE and DICER gene families in colon cancer.Methods The expression of the human ARGONAUTE and DICER gene family in colon cancer tissues and adjacent tissueswere determined by real-time fluorescence quantitative polymerase chain reaction(PCR). Results The expression of AGO2 was significantly up-regulated,the expression of other AGO genes was down-regulated,and the expression of DICER familywas significantly down-regulated. The differences were statistically significant(P<0.05). Conclusion Abnormal expression ofthe ARGONAUTE and DICER families suggests that they may be involved in the development of colon cancer.
Objective To analyze the expression of human ARGONAUTE and DICER gene families in colon cancer.Methods The expression of the human ARGONAUTE and DICER gene family in colon cancer tissues and adjacent tissueswere determined by real-time fluorescence quantitative polymerase chain reaction(PCR). Results The expression of AGO2 was significantly up-regulated,the expression of other AGO genes was down-regulated,and the expression of DICER familywas significantly down-regulated. The differences were statistically significant(P<0.05). Conclusion Abnormal expression ofthe ARGONAUTE and DICER families suggests that they may be involved in the development of colon cancer.
引文
[1]刘见荣,侯风刚.结肠癌治疗概况[J].辽宁中医药大学学报,2014,16(2):99-101.
[2]张兰,张德志.天胡荽的研究进展[J].现代食品与药品杂志,2007,17(1):15-17.
[3]SONG JJ,STEPHANIE KS,GREGORY JH,et al.Crystal structure of Argenaute and its in plications for RISC slicer activity[J].Science,2004,305(5689):1434-1437.
[4]CHOE J,CHO H,LEE HC,et al.Microrna/ARGONAUTE 2 regulates nonsense-mediated messenger rna decay[J].EMBO Rep,2010,11(5):380-386.
[5]YANG FQ,HUANG JH,LIU H,et al.ARGONAUTE 2 is up-regulated in tissues of urothelial carcinoma of bladder[J].Int J Clin Exp Pathol,2014,7(1):340-347.
[6]STEVEN SC,IAN S,CHAD AG,et al.EIF2C is overexpressed and amplified in head and neck squamous cell carcinoma[J].ORL,2010,72(6):337-343.
[7]OLGA V,THEA EH,CLAES GT,et al.ARGONAUTE,DICER,and drosha are up-regulated along tumor progression in serous ovarian carcinoma[J].Human Pathol,2012,43(11):2062-2069.
[8]ZHANG J,JIN H,LIU H,et al.Mirna-99a directly regulates ago2through translational repression in hepatocellular carcinoma[J].Oncogenesis,2014,4(3):e97.
[9]BO F,PENG H,LU SJ,et al.Increased ARGONAUTE 2 expression in gliomas and its association with tumor progression and poor prognosis[J].Asian Pac J Cancer P,2014,15(9):4079-4083.
[10]DANIEL V,JORG R,GUNTER M,et al.Strong reduction of AGO2 expression in melanoma and cellular consequences[J].Bri J Cancer,2013,109(12):3116-3124.
[11]SUDHIR GT,THOMAS R,SARAH JM,et al.ARGONAUTE2 mediates compensatory expansion of the pancreatic beta cell[J].Cell metabolism,2014,19(1):122-134.
[12]ZHANG XX,GRAVES P,ZENG Y.Overexpression of human ARGONAUTE2 inhibits cell and tumor growth[J].Bioch Bioph Acta,2013,1830(3):2553-2561.
[13]JOSIE H,PIERPAOLO P,SEAN EL.Micro RNAs in cancer biomarkers,functions and therapy[J].Trends Mol Med,2014,20(8):460-469.
[14]LI ZH,TARIQ MR.Therapeutic targeting of micro RNAs:current status and future challenges[J].Nat rev Drug Discov,2014,13(8):622-638.
[15]SHANE RH,MAJA MJ,CLAUDIA L,et al.Akt-mediated phosphorylation of ARGONAUTE 2 downregulates cleavage and upregulates translational repression of Micro RNA targets[J].Mol Cell,2013,50(3):356-367.
[16]VERA H,JIASHUN Z,ZHONGXIA Q,et al.Ago1 interacts with RNA polymeraseⅡand binds to the promoters of actively transcribed genes in human cancer cells[J].PLo S Genetics,2013,9(9):e1003821.
[17]VOLLER D,LINCK L,BRUCKMANN A,et al.ARGONAUTE Family Protein Expression in Normal Tissue and Cancer Entities[J].PLo S One,2016,11(8):e0161165.
[18]WANG DW,WANG ZH,WANG LL,et al.Overexpression of hiwi promotes growth of human breast cancer cells[J].Asian Pac J Cancer Prev,2014,9(15):7553-7558.
[19]CAO J,XU G,LAN J,et al.High expres-sion of piwi-like rna mediated gene silencing 1 is associated with poor prognosis via regulating transforming growth factor-a receptors and cyclin-dependent kinases in breast cancer[J].Mol Med Rep,2016,13(3):2829-2835.
[20]WANG Y,LIU Y,SHEN X,et al.The piwi protein acts as a predictive marker for human gastric cancer[J].Int J Clin Exp Pathol,2012,5(4):315-325.
[21]HE W,WANG Z,WANG Q,et al.Expression of HIWI in human esopha-geal squamous cell carcinoma is significantly associated with poorer prognosis[J].BMC Cancer,2009,9(1):426-428.
[22]ZHENG C,QI C,XIAOYING H,et al.Stem cell protein Piwil1 endowed endometrial cancer cells with stem-like properties via inducing epithelial-mesenchymal transition[J].BMC Cancer,2015,15(1):811.
[23]范正军,杨飞龙,孙涛,等.PIWIL1在肝细胞癌中的表达及临床意义[J].世界华人消化杂志,2014,22(1):119-124.
[24]TAUBERT H,WURL P,GREITHER T,et al.Stem cell-associated genes are extremely poor prognostic factors for soft-tissue sarcoma patients[J].Oncogene,2007,26(1):7170-7174.
[25]GROCHOLA LF,GREITHER T,TAUBERT H,et al.The stem cell-associated Hiwi gene in human ad-enocarcinoma of the pancreas:expression and risk of tumour-related death[J].Br J Cancer,2008,99(7):1083-1088.
[26]田瑞卿,刘民,李欣,等.PIWI1在不用肿瘤中的差异性表达[J].中国生物化学和分子生物学报,2008,24(11):1072-1075.
[27]GANG H,LI C,YIN Y,et al.Piwil2 expressed in various stages of cervical neoplasia is a potential complementary marker for p16INK4a[J].Am J Trans Res,2010,2(2):156-169.
[28]JAMES JL,RULONG S,LI C,et al.Piwil2 is expressed in various stages of breast cancers and has the potential to be used as a novel biomarker[J].Int J Clin Exp Pathol,2010,3(4):328-337.
[29]WANG Y,LIU J,WU GA,et al.Manipulations in hiwi levels exerts influence on the proliferation of human non-small cell lung cancer cells[J].Exp Ther Med,2016,11(1):1971-1976.
[30]XIAOHAN Q,JINLU L,XINWEN Z,et al.PIWIL2 promotes progression of non-small cell lung cancer by inducing CDK2 and Cyclin A expression-PIWIL2[J].J Trans Med,2015,13(2):301-302.
[31]ZIFENG W,NA L,SHUO S,et al.The role of PIWIL4,an ARGONAUTE family protein,in breast cancer[J].J Bio Chem,2016,291(20):10646-10658.
[32]曹政.DICER和Drosha在骨肉瘤和骨软骨瘤中的表达及意义[D].长沙:中南大学,2010:1-5.
[33]YAN M,HUANG HY,WANG T,et al.Dysregulated expression of DICER and drosha in breast cancer[J].Pathol Oncol Res,2012,18(2):343-348.
[34]TORRES A,TORRES K.Major regulators of micro RNAs biogenesis DICER and Drosha are down-regulated in endometrial cancer[J].Tumor Biol,2011,32(4):769-776.
[35]XIAOFANG G,QIANJIN L,PAN C,et al.The micro RNA-processing enzymes:Drosha and DICER can predict prognosis of nasopharyngeal carcinoma[J].J Cancer Res Clin Oncol,2012,138(1):49-56.
[36]RUEYJEN L,YOUCHIN L,JEREMY JW,et al.Micro RNA signature and expression of DICER and drosha can predict prognosis and delineate risk groups in neuroblastoma[J].Cancer Res,2010,70(20):7841-7850.
[37]FABER C,HORSTA D,HLUBEK F,et al.Overexpression of DICER predicts poor survival in colorectal cancer[J].Euro J Cancer,2011,47(9):1414-1419.
[38]YOKO K,HISAAKI T,HIROTAKA O,et al.Reduced expression of DICER associated with poor prognosis in lung cancer patients[J].Cancer Sci,2005,96(2):111-115.
[39]WILLIAM MM,YVONNE GL,LIZ YH,et al.DICER,drosha,and outcomes in patients with ovarian cancer[J].New Eng J Med,2008,359(25):2641-2650.
[2]张兰,张德志.天胡荽的研究进展[J].现代食品与药品杂志,2007,17(1):15-17.
[3]SONG JJ,STEPHANIE KS,GREGORY JH,et al.Crystal structure of Argenaute and its in plications for RISC slicer activity[J].Science,2004,305(5689):1434-1437.
[4]CHOE J,CHO H,LEE HC,et al.Microrna/ARGONAUTE 2 regulates nonsense-mediated messenger rna decay[J].EMBO Rep,2010,11(5):380-386.
[5]YANG FQ,HUANG JH,LIU H,et al.ARGONAUTE 2 is up-regulated in tissues of urothelial carcinoma of bladder[J].Int J Clin Exp Pathol,2014,7(1):340-347.
[6]STEVEN SC,IAN S,CHAD AG,et al.EIF2C is overexpressed and amplified in head and neck squamous cell carcinoma[J].ORL,2010,72(6):337-343.
[7]OLGA V,THEA EH,CLAES GT,et al.ARGONAUTE,DICER,and drosha are up-regulated along tumor progression in serous ovarian carcinoma[J].Human Pathol,2012,43(11):2062-2069.
[8]ZHANG J,JIN H,LIU H,et al.Mirna-99a directly regulates ago2through translational repression in hepatocellular carcinoma[J].Oncogenesis,2014,4(3):e97.
[9]BO F,PENG H,LU SJ,et al.Increased ARGONAUTE 2 expression in gliomas and its association with tumor progression and poor prognosis[J].Asian Pac J Cancer P,2014,15(9):4079-4083.
[10]DANIEL V,JORG R,GUNTER M,et al.Strong reduction of AGO2 expression in melanoma and cellular consequences[J].Bri J Cancer,2013,109(12):3116-3124.
[11]SUDHIR GT,THOMAS R,SARAH JM,et al.ARGONAUTE2 mediates compensatory expansion of the pancreatic beta cell[J].Cell metabolism,2014,19(1):122-134.
[12]ZHANG XX,GRAVES P,ZENG Y.Overexpression of human ARGONAUTE2 inhibits cell and tumor growth[J].Bioch Bioph Acta,2013,1830(3):2553-2561.
[13]JOSIE H,PIERPAOLO P,SEAN EL.Micro RNAs in cancer biomarkers,functions and therapy[J].Trends Mol Med,2014,20(8):460-469.
[14]LI ZH,TARIQ MR.Therapeutic targeting of micro RNAs:current status and future challenges[J].Nat rev Drug Discov,2014,13(8):622-638.
[15]SHANE RH,MAJA MJ,CLAUDIA L,et al.Akt-mediated phosphorylation of ARGONAUTE 2 downregulates cleavage and upregulates translational repression of Micro RNA targets[J].Mol Cell,2013,50(3):356-367.
[16]VERA H,JIASHUN Z,ZHONGXIA Q,et al.Ago1 interacts with RNA polymeraseⅡand binds to the promoters of actively transcribed genes in human cancer cells[J].PLo S Genetics,2013,9(9):e1003821.
[17]VOLLER D,LINCK L,BRUCKMANN A,et al.ARGONAUTE Family Protein Expression in Normal Tissue and Cancer Entities[J].PLo S One,2016,11(8):e0161165.
[18]WANG DW,WANG ZH,WANG LL,et al.Overexpression of hiwi promotes growth of human breast cancer cells[J].Asian Pac J Cancer Prev,2014,9(15):7553-7558.
[19]CAO J,XU G,LAN J,et al.High expres-sion of piwi-like rna mediated gene silencing 1 is associated with poor prognosis via regulating transforming growth factor-a receptors and cyclin-dependent kinases in breast cancer[J].Mol Med Rep,2016,13(3):2829-2835.
[20]WANG Y,LIU Y,SHEN X,et al.The piwi protein acts as a predictive marker for human gastric cancer[J].Int J Clin Exp Pathol,2012,5(4):315-325.
[21]HE W,WANG Z,WANG Q,et al.Expression of HIWI in human esopha-geal squamous cell carcinoma is significantly associated with poorer prognosis[J].BMC Cancer,2009,9(1):426-428.
[22]ZHENG C,QI C,XIAOYING H,et al.Stem cell protein Piwil1 endowed endometrial cancer cells with stem-like properties via inducing epithelial-mesenchymal transition[J].BMC Cancer,2015,15(1):811.
[23]范正军,杨飞龙,孙涛,等.PIWIL1在肝细胞癌中的表达及临床意义[J].世界华人消化杂志,2014,22(1):119-124.
[24]TAUBERT H,WURL P,GREITHER T,et al.Stem cell-associated genes are extremely poor prognostic factors for soft-tissue sarcoma patients[J].Oncogene,2007,26(1):7170-7174.
[25]GROCHOLA LF,GREITHER T,TAUBERT H,et al.The stem cell-associated Hiwi gene in human ad-enocarcinoma of the pancreas:expression and risk of tumour-related death[J].Br J Cancer,2008,99(7):1083-1088.
[26]田瑞卿,刘民,李欣,等.PIWI1在不用肿瘤中的差异性表达[J].中国生物化学和分子生物学报,2008,24(11):1072-1075.
[27]GANG H,LI C,YIN Y,et al.Piwil2 expressed in various stages of cervical neoplasia is a potential complementary marker for p16INK4a[J].Am J Trans Res,2010,2(2):156-169.
[28]JAMES JL,RULONG S,LI C,et al.Piwil2 is expressed in various stages of breast cancers and has the potential to be used as a novel biomarker[J].Int J Clin Exp Pathol,2010,3(4):328-337.
[29]WANG Y,LIU J,WU GA,et al.Manipulations in hiwi levels exerts influence on the proliferation of human non-small cell lung cancer cells[J].Exp Ther Med,2016,11(1):1971-1976.
[30]XIAOHAN Q,JINLU L,XINWEN Z,et al.PIWIL2 promotes progression of non-small cell lung cancer by inducing CDK2 and Cyclin A expression-PIWIL2[J].J Trans Med,2015,13(2):301-302.
[31]ZIFENG W,NA L,SHUO S,et al.The role of PIWIL4,an ARGONAUTE family protein,in breast cancer[J].J Bio Chem,2016,291(20):10646-10658.
[32]曹政.DICER和Drosha在骨肉瘤和骨软骨瘤中的表达及意义[D].长沙:中南大学,2010:1-5.
[33]YAN M,HUANG HY,WANG T,et al.Dysregulated expression of DICER and drosha in breast cancer[J].Pathol Oncol Res,2012,18(2):343-348.
[34]TORRES A,TORRES K.Major regulators of micro RNAs biogenesis DICER and Drosha are down-regulated in endometrial cancer[J].Tumor Biol,2011,32(4):769-776.
[35]XIAOFANG G,QIANJIN L,PAN C,et al.The micro RNA-processing enzymes:Drosha and DICER can predict prognosis of nasopharyngeal carcinoma[J].J Cancer Res Clin Oncol,2012,138(1):49-56.
[36]RUEYJEN L,YOUCHIN L,JEREMY JW,et al.Micro RNA signature and expression of DICER and drosha can predict prognosis and delineate risk groups in neuroblastoma[J].Cancer Res,2010,70(20):7841-7850.
[37]FABER C,HORSTA D,HLUBEK F,et al.Overexpression of DICER predicts poor survival in colorectal cancer[J].Euro J Cancer,2011,47(9):1414-1419.
[38]YOKO K,HISAAKI T,HIROTAKA O,et al.Reduced expression of DICER associated with poor prognosis in lung cancer patients[J].Cancer Sci,2005,96(2):111-115.
[39]WILLIAM MM,YVONNE GL,LIZ YH,et al.DICER,drosha,and outcomes in patients with ovarian cancer[J].New Eng J Med,2008,359(25):2641-2650.