口腔鳞癌中EIF3e和MMP-12的表达意义
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摘要
目的:检测口腔鳞癌组织中真核翻译起始因子(EIF3e)和基质金属蛋白酶-12(MMP-12)的表达,分析其临床价值。方法:选择83例口腔鳞癌作为观察组,39例正常口腔鳞状上皮组织作为对照组。应用免疫组织化学法检测EIF3e和MMP-12蛋白的表达。结果:与对照组比较,观察组中EIF3e和MMP-12的表达明显增高。观察组中EIF3e和MMP-12的表达在不同淋巴结转移和脉管累犯中相比差异具有统计学意义,EIF3e的表达在不同Ki67指数和肿瘤最大径中相比差异具有统计学意义,MMP-12的表达在不同间质炎症细胞浸润程度中相比差异具有统计学意义(P <0. 05)。观察组中EIF3e和MMP-12的表达呈正相关性。相关分析显示二者的表达与预后相关。结论:EIF3e和MMP-12在口腔鳞癌中表达升高,二者具有正相关性,可以促进病变的形成和进展。二者联合检测对判断预后有一定价值
Objective: To detect the expressions of eukaryotic translation initiation factor 3e(EIF3e) and matrix metalloprotein-12(MMP-12) and investigate their clinical significance in oral squamous carcinoma. Methods: The study included 83 cases of oral squamous carcinoma as observation group and 39 cases of normal oral squamous epithelium as control group. The expressions of EIF3e and MMP-12 were detected by immunohistochemistry methods in two groups. Results: The expression of EIP3e and MMP-12 was obviously higher in observation group than in control group. Expression of EIF3e and MMP-12 was obviously different in lymph node metastasis and vascular recidivism. Expression of EIF3e was obviously different in Ki67 and maximum diameter. Expression of MMP-12 was obviously different in inflammation infiltration. Positive correlation was found between EIF3e and MMP-12 in observation group by linear analysis. Their expressions were related with prognosis. Conclusion: The higher expression and positive correlation of EIF3e and MMP-12 may promote occurrence and development in oral squamous carcinoma. Combined detection of EIF3e and MMP-12 will be useful to judge prognosis in oral squamous cell carcinoma.
Objective: To detect the expressions of eukaryotic translation initiation factor 3e(EIF3e) and matrix metalloprotein-12(MMP-12) and investigate their clinical significance in oral squamous carcinoma. Methods: The study included 83 cases of oral squamous carcinoma as observation group and 39 cases of normal oral squamous epithelium as control group. The expressions of EIF3e and MMP-12 were detected by immunohistochemistry methods in two groups. Results: The expression of EIP3e and MMP-12 was obviously higher in observation group than in control group. Expression of EIF3e and MMP-12 was obviously different in lymph node metastasis and vascular recidivism. Expression of EIF3e was obviously different in Ki67 and maximum diameter. Expression of MMP-12 was obviously different in inflammation infiltration. Positive correlation was found between EIF3e and MMP-12 in observation group by linear analysis. Their expressions were related with prognosis. Conclusion: The higher expression and positive correlation of EIF3e and MMP-12 may promote occurrence and development in oral squamous carcinoma. Combined detection of EIF3e and MMP-12 will be useful to judge prognosis in oral squamous cell carcinoma.
引文
[1]Wagner VP,Webber LP,Salvadori G,et al.Overexpression of Mut Sαcomplex proteins predicts poor prognosis in oral squamous cell carcinoma[J].Medicine,2016,95(22):e3725.
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[5]Kaneko T,Dehari H,Sasaki T,et al.Hypoxia-induced epithelialmesenchymal transition is regulated by phosphorylation of GSK3-βvia PI3K/Akt signaling in oral squamous cell carcinoma[J].Oral Sur Oral Med Oral Pathol Oral Radiol,2016,122(6):719-730.
[6]Kahlert C,Fiala M,Musso G,et al.Prognostic impact of a compartment-specific angiogenic marker profile in patients with pancreatic cancer[J].Oncotarget,2014,5(24):12978-12989.
[7]Mack DL,Boulanger CA,Callahan R,et al.Expression of truncated Int6/e IF3e in mammary alveolar epithelium leads to persistent hyperplasia and tumorigenesis[J].Br Cancer Res Bcr,2007,9(4):42-44.
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[9]Yahalom A,Kim TH,Winter E,et al.Arabidopsis e IF3e(INT-6)associates with both e IF3c and the COP9 signalosome subunit CSN7[J].J Biol Chem,2001,276(1):334-340.
[10]Han F,Zhang S,Zhang L,et al.The overexpression and predictive significance of MMP-12 in esophageal squamous cell carcinoma[J].Pathol Res Pract,2017,213(12):1519-1522.
[11]Park JW,Kim YJ,Shin IS,et al.TypeⅢsecretion system of pseudomonas aeruginosa affects MMP-12 and MMP-13 expression via NF-κB signaling in human carcinoma epithelial cells and a pneumonia mouse model[J].J Infect Dis,2016,214(6):962-963.
[12]Jormsjo S,Ye S,Moritz J,et al.Allele-specific regulation of matrix metalloproteinase-12 gene activity is associated with coronary artery luminal dimensions in diabetic patients with manifest coronary artery disease[J].Circ Res,2000,86(9):998-1003.
[13]Sesen J,Cammas A,Scotland SJ,et al.Int6/e IF3e is essential for proliferation and survival of human glioblastoma cells[J].Int JMol Sci,2014,15(2):2172-2190.
[14]Li C,Endler A,Uchida K,et al.Int6/e IF3e silencing promotes functional blood vessel outgrowth and enhances wound healing by upregulating hypoxia-induced factor 2αexpression[J].Circulation,2010,122(9):910-919.
[15]Kim JM,Kim HJ,Koo BS,et al.Expression of matrix metalloproteinase-12 is correlated with extracapsular spread of tumor from nodes with metastasis in head and neck squamous cell carcinoma[J].Eur Arch Otorhinolaryngol,2013,270(3)1137-1142.
[16]Han F,Zhang S,Zhang L,et al.The overexpression and predictive significance of MMP-12 in esophageal squamous cell carcinoma[J].Pathol Res Pract,2017,213(12)1519-1522.
[2]Grzmil M,Rzymski T,Milani M,et al.An oncogenic role of e IF3e/INT6 in human breast cancer[J].Oncogene,2010,29(28):4080-4089.
[3]Cho NH,Hong KP,Hong SH,et al.MMP expression profiling in recurred stage IB lung cancer[J].Oncogene,2015,23(3):845-851.
[4]Wells JE,Biernaskie J,Szymanska A,et al.Matrix metalloproteinase(MMP)-12 expression has a negative impact on sensorimotor function following intracerebral haemorrhage in mice[J].Eur J Neurosci,2015,21(1):187-196.
[5]Kaneko T,Dehari H,Sasaki T,et al.Hypoxia-induced epithelialmesenchymal transition is regulated by phosphorylation of GSK3-βvia PI3K/Akt signaling in oral squamous cell carcinoma[J].Oral Sur Oral Med Oral Pathol Oral Radiol,2016,122(6):719-730.
[6]Kahlert C,Fiala M,Musso G,et al.Prognostic impact of a compartment-specific angiogenic marker profile in patients with pancreatic cancer[J].Oncotarget,2014,5(24):12978-12989.
[7]Mack DL,Boulanger CA,Callahan R,et al.Expression of truncated Int6/e IF3e in mammary alveolar epithelium leads to persistent hyperplasia and tumorigenesis[J].Br Cancer Res Bcr,2007,9(4):42-44.
[8]Li Z,Lin S,Jiang T,et al.Overexpression of e IF3e is correlated with colon tumor development and poor prognosis[J].Int J Clin Exp Pathol,2014,35(10):6462-6474.
[9]Yahalom A,Kim TH,Winter E,et al.Arabidopsis e IF3e(INT-6)associates with both e IF3c and the COP9 signalosome subunit CSN7[J].J Biol Chem,2001,276(1):334-340.
[10]Han F,Zhang S,Zhang L,et al.The overexpression and predictive significance of MMP-12 in esophageal squamous cell carcinoma[J].Pathol Res Pract,2017,213(12):1519-1522.
[11]Park JW,Kim YJ,Shin IS,et al.TypeⅢsecretion system of pseudomonas aeruginosa affects MMP-12 and MMP-13 expression via NF-κB signaling in human carcinoma epithelial cells and a pneumonia mouse model[J].J Infect Dis,2016,214(6):962-963.
[12]Jormsjo S,Ye S,Moritz J,et al.Allele-specific regulation of matrix metalloproteinase-12 gene activity is associated with coronary artery luminal dimensions in diabetic patients with manifest coronary artery disease[J].Circ Res,2000,86(9):998-1003.
[13]Sesen J,Cammas A,Scotland SJ,et al.Int6/e IF3e is essential for proliferation and survival of human glioblastoma cells[J].Int JMol Sci,2014,15(2):2172-2190.
[14]Li C,Endler A,Uchida K,et al.Int6/e IF3e silencing promotes functional blood vessel outgrowth and enhances wound healing by upregulating hypoxia-induced factor 2αexpression[J].Circulation,2010,122(9):910-919.
[15]Kim JM,Kim HJ,Koo BS,et al.Expression of matrix metalloproteinase-12 is correlated with extracapsular spread of tumor from nodes with metastasis in head and neck squamous cell carcinoma[J].Eur Arch Otorhinolaryngol,2013,270(3)1137-1142.
[16]Han F,Zhang S,Zhang L,et al.The overexpression and predictive significance of MMP-12 in esophageal squamous cell carcinoma[J].Pathol Res Pract,2017,213(12)1519-1522.