AtDREB1A基因过量表达对马铃薯盛花期生理生化指标的影响
|
摘要
以马铃薯品种陇薯3号及其转AtDREB1A基因株系T1为研究对象,在干旱胁迫条件下,研究了AtDREB1A基因过量表达对盛花期生理生化指标的影响。分析表明,在不同程度的干旱胁迫下,转基因株系T1盛花期叶片的超氧化物歧化酶(SOD)活性及脯氨酸(Pro)含量均高于同期非转基因陇薯3号对照,而丙二醛(MDA)含量和相对电导率均低于陇薯3号。以上结果初步表明,AtDREB1A基因的过量表达明显提高了马铃薯对干旱的抗性。
In order to study the effects of excessive expression of AtDREB1 A gene on physiological and biochemical indexes of potato at flowering stage,the cultivar of potato Longshu 3 and its AtDREB1 A genetic strain T1 is used as materials and the activity of SOD,the content of Pro,the content of MDA and the electrical conductivity in leaves are analyzed. The result shows that the activity of SOD and the content of Pro in the transgenic potato were higher than those in the non-transgenic control Longshu 3,and the content of MDA and the electrical conductivity in transgenic potato are lower than those of Longshu 3. The result shows that the excessive expression of AtDREB1 A gene in potato improved the potato drought-resistance.
In order to study the effects of excessive expression of AtDREB1 A gene on physiological and biochemical indexes of potato at flowering stage,the cultivar of potato Longshu 3 and its AtDREB1 A genetic strain T1 is used as materials and the activity of SOD,the content of Pro,the content of MDA and the electrical conductivity in leaves are analyzed. The result shows that the activity of SOD and the content of Pro in the transgenic potato were higher than those in the non-transgenic control Longshu 3,and the content of MDA and the electrical conductivity in transgenic potato are lower than those of Longshu 3. The result shows that the excessive expression of AtDREB1 A gene in potato improved the potato drought-resistance.
引文
[1]LIU Q,KASUGA M.Two transcription factors,DREBl and DREB2,with an EREBP/AP2 DNA binding domain separate two cellular signal transduetion pathways in drought-and low-temperature-responsive gene expression,respectively,in Arabidopsis[J].Plant Cell,1998,10:1391-1406.
[2]KASUGA M,MIURA S,SHINOZAKI K.,et a1.A combination of the Arabidopsis DREB1A gene and stress-inducible rd29A promoter improved drought and low-temperature stress tolerance in tobacco by gene transfer[J].Plant and Cell Physiology,2004,45(3):346-350.
[3]LIU L X,ZHAO L S,LIANG X X.et al.Study on production of transgenic wheat with a stress-inducible transcription factor gene REEB1A by microprojectile bombardment[J].China Biotechnology,2003,23(11):53-56.
[4]PELLEGRINESCHI A.,REYNOLDS M.,PACHECO M.,et a1.Stress-induced expression in wheat of the Arobidopsls thaliana DREB1A gene delays water stress symptoms under greenhouse conditions[J].Gencme,2004,47(3):493-500.
[5]许昆朋.转CBF3基因马铃薯提高抗冷性的研究[D].泰安:山东农业大学,2011.
[6]DOUH O,XVK P,MENGQ W,et a1.Potato plants ectopically expressing Arabidopsis thaliana CBF3 exhibit enhanced tolerance to high-temperature stress[J].Plant,Cell and Environment,2014,38:61-72.
[7]贾小霞,齐恩芳,王一航,等.转录因子DREB1A基因和Bar基因双价植物表达载体的构建及对马铃薯遗传转化的研究[J].草业学报,2014,23(3):110-114.
[8]高文俊,徐静,谢开云,等.Na2CO3和Na HCO3胁迫下冰草的生长及生理响应[J].草业学报,2011,20(4):299-304.
[9]李源,刘贵波,高洪文,等.紫花苜蓿种质耐盐性综合评价及盐胁迫下的生理反应[J].草业学报,2010,19(4):79-86.
[10]张海娜,李小娟,李存东,等.过量表达小麦超氧化物歧化酶基因对烟草耐盐能力的影响[J].作物学报,2008,34(8):1403-1408.
[2]KASUGA M,MIURA S,SHINOZAKI K.,et a1.A combination of the Arabidopsis DREB1A gene and stress-inducible rd29A promoter improved drought and low-temperature stress tolerance in tobacco by gene transfer[J].Plant and Cell Physiology,2004,45(3):346-350.
[3]LIU L X,ZHAO L S,LIANG X X.et al.Study on production of transgenic wheat with a stress-inducible transcription factor gene REEB1A by microprojectile bombardment[J].China Biotechnology,2003,23(11):53-56.
[4]PELLEGRINESCHI A.,REYNOLDS M.,PACHECO M.,et a1.Stress-induced expression in wheat of the Arobidopsls thaliana DREB1A gene delays water stress symptoms under greenhouse conditions[J].Gencme,2004,47(3):493-500.
[5]许昆朋.转CBF3基因马铃薯提高抗冷性的研究[D].泰安:山东农业大学,2011.
[6]DOUH O,XVK P,MENGQ W,et a1.Potato plants ectopically expressing Arabidopsis thaliana CBF3 exhibit enhanced tolerance to high-temperature stress[J].Plant,Cell and Environment,2014,38:61-72.
[7]贾小霞,齐恩芳,王一航,等.转录因子DREB1A基因和Bar基因双价植物表达载体的构建及对马铃薯遗传转化的研究[J].草业学报,2014,23(3):110-114.
[8]高文俊,徐静,谢开云,等.Na2CO3和Na HCO3胁迫下冰草的生长及生理响应[J].草业学报,2011,20(4):299-304.
[9]李源,刘贵波,高洪文,等.紫花苜蓿种质耐盐性综合评价及盐胁迫下的生理反应[J].草业学报,2010,19(4):79-86.
[10]张海娜,李小娟,李存东,等.过量表达小麦超氧化物歧化酶基因对烟草耐盐能力的影响[J].作物学报,2008,34(8):1403-1408.