转录因子DREB1A基因的克隆及植物表达载体的构建
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摘要
研究转录因子DREB1A在植物抗渗透胁迫反应中的作用。根据GenBank中登录的DREB1A基因的序列设计引物,用PCR方法从拟南芥中克隆DREB1A基因,利用基因重组技术成功构建了植物表达载体pCAMB1A1300-DREB1A。该结果为进一步利用DREB1A基因做遗传转化研究奠定了基础。
To research the function of the transcription factor DREB1 A in plant tolerance to osmotic stress, a pair of primers was designed according to the sequence of DREB1 A gene in GenBank databases. The DREB1 A gene was cloned from Arabidopsis thaliarra by the method of PCR. By the method of Recombinant DNA Technology, plant expression vector pCAMB1 A1300-DREB1 A was successfully constructed. The results laid a foundation for further research on genetic transformation using DREB1 A gene.
To research the function of the transcription factor DREB1 A in plant tolerance to osmotic stress, a pair of primers was designed according to the sequence of DREB1 A gene in GenBank databases. The DREB1 A gene was cloned from Arabidopsis thaliarra by the method of PCR. By the method of Recombinant DNA Technology, plant expression vector pCAMB1 A1300-DREB1 A was successfully constructed. The results laid a foundation for further research on genetic transformation using DREB1 A gene.
引文
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[2]倪志勇,徐兆师,李连城,等. DREB转录因子在植物逆境胁迫中的作用机理及应用研究进展[J].麦类作物学报,2008,28(6):1100-1106.
[3]张永恩,李潮海,王群.植物抗旱相关基因研究进展[J].中国农学通报,2004,20(6):85-88.
[4] YAMAGUCHI-SHINOZAKI K,SHINOZAKI K. A novel cisacting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or highsalt stress[J]. Plan Cell,1994,6(2):251-264.
[5]张腾国,郭艳峰,陈琼琼,等.油菜COR基因的克隆及原核表达[J].甘肃农业科技,2015(4):1-4.
[6]孙立平,李德全. LEA蛋白的分子生物学研究进展[J].生物技术通报,2003(6):5-13.
[7]刘强,赵南明,YAMAGUCHI-SHINOZAKI K,等.DREB转录因子在提高植物抗逆性的作用[J].科学通报,2000,45(1):11-16.
[8]王艳青,陈雪梅,李悦,等.植物抗逆中的渗透调节物质及其转基因工程进展[J].北京林业大学学报,2001,23(4):66-70.
[9]倪志勇,马文静,吕萌,等.棉花肉桂醇脱氢酶基因GhCAD6的克隆及正义、反义与RNAi干扰载体的构建[J].华北农学报,2009,24(6):20-26.
[10]贾小霞,文国宏,李高峰,等.拟南芥DREB1A基因的克隆与植物表达载体构建[J].甘肃农业科技,2011(6):18-21.
[11]奥斯伯F,金斯顿R F,布伦特R,等.精编分子生物学实验指南[M].颜子颖,王海林,译.北京:科学出版社,1999.