去氢骆驼蓬碱磁固体脂质纳米粒的处方和制备工艺的优化
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摘要
目的:比较去氢骆驼蓬碱磁固体脂质纳米粒(HM-MSLN)的不同制备方法,以单因素考察结合星点设计响应面法优化制备工艺。方法:比较薄膜超声分散法、微乳超声法、乳化低温固化法、乳化溶剂挥发法制备HM-MSLN,筛选得到粒径小、载药量高、稳定性好的制备方法;再以单因素考察结合星点设计响应面法优化处方与制备工艺。结果:优选的薄膜超声分散法所制备的HM-MSLN粒径为(178.00±6.07) nm,包封率为(95.38±1.08)%,载药量为(4.21±0.03)%。结论:薄膜超声分散法所制备的HM-MSLN具有粒径小、载药量较高、制备工艺简单等特点。
Objective: To compare the different preparation methods of harmine magnetic solid lipid nanoparticles(HMMSLN) and optimize the preparation process by single factor analysis combined with star point design response surface method.Methods: Four kinds of preparation methods of HM-MSLN including thin film ultrasonic dispersion method, microemulsion ultrasonic method, emulsification low temperature curing method and emulsion solvent evaporation method were compared to obtain the best preparations method with small particle size, high drug loading and good stability. And single factor analysis and star point design response surface method were further adopted to optimize the prescription and preparation. Results: HM-MSLN prepared by the preferred method of film ultrasonic dispersion had a particle diameter of(178.00 ± 6.07) nm, an entrapment rate of(95.38±1.08)%, and a drug-loading rate of(4.21 ± 0.03)%. Conclusion: HM-MSLN prepared by thin film ultrasonic dispersion had the characteristics of small particle size, high drug loading and simple preparation process.
Objective: To compare the different preparation methods of harmine magnetic solid lipid nanoparticles(HMMSLN) and optimize the preparation process by single factor analysis combined with star point design response surface method.Methods: Four kinds of preparation methods of HM-MSLN including thin film ultrasonic dispersion method, microemulsion ultrasonic method, emulsification low temperature curing method and emulsion solvent evaporation method were compared to obtain the best preparations method with small particle size, high drug loading and good stability. And single factor analysis and star point design response surface method were further adopted to optimize the prescription and preparation. Results: HM-MSLN prepared by the preferred method of film ultrasonic dispersion had a particle diameter of(178.00 ± 6.07) nm, an entrapment rate of(95.38±1.08)%, and a drug-loading rate of(4.21 ± 0.03)%. Conclusion: HM-MSLN prepared by thin film ultrasonic dispersion had the characteristics of small particle size, high drug loading and simple preparation process.
引文
[1]王梅林,牛精铃,高杨,等.去氢骆驼蓬碱对MCF7细胞增殖与迁移侵袭的影响[J].中药材,2018,41(7):1703-1707.
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[11]李海珍,胡彦武,姚慧敏,等.固体脂质纳米粒及其脑靶向作用的研究进展[J].通化师范学院学报,2018,39(4):43-49.
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[13]蔡晓瑶,张莉,任翔,等.离心法和透析法测定蟾皮提取物固体脂质纳米粒包封率的比较[J].国际药学研究杂志,2016,43(4):744-747.
[14]严春临,张季,刘敏,等.星点设计-效应面法优化吴茱萸次碱固体脂质纳米粒处方[J].中草药,2015,46(9):1307-1313.
[2]刘诗伶.去氢骆驼蓬碱对宫颈癌的抑制作用及其分子机制研究[D].江西:南昌大学,2018.
[3]李船.去氢骆驼蓬碱诱导人胃癌细胞MGC-803及SGC-7901自噬及凋亡的机制研究[D].广东:广东药科大学,2017.
[4]Li C, Wang Y, Wang C, et al. Anticancer activities of harmine by inducing a pro-death autophagy and apoptosis in human gastric cancer cells[J]. Phytomedicine, 2017, 28:10-18.
[5]夏衍.去氢骆驼蓬碱衍生物生物活性、作用机理及构效关系研究[D].广东:华南农业大学,2016.
[6]李蕊,王梅.去氢骆驼蓬碱长循环磁纳米脂质体的制备及体外性质的研究[J].西北药学杂志,2017,32(2):181-184.
[7]唐小慧,木尼热·库尔班,焦敏,等.转铁蛋白修饰的去氢骆驼蓬碱磁纳米脂质体的制备及抑瘤效果评价[J].中国药学杂志,2018,53(11):900-905.
[8]黄川生,王新春,王鲁妹,等.去氢骆驼蓬碱固体脂质纳米粒包封率测定方法研究[J].中成药,2011,33(3):516-518.
[9]黄川生,刘恒戈,王新春,等.去氢骆驼蓬碱固体脂质纳米粒制备工艺的研究[J].农垦医学,2010,32(6):487-489.
[10]高艺敏,张震,陈佩敏,等.乳化挥发法和薄膜-超声法制备阿魏酸固体脂质纳米粒及其工艺比较[J].食品与发酵工业,2019,45(6):127-132.
[11]李海珍,胡彦武,姚慧敏,等.固体脂质纳米粒及其脑靶向作用的研究进展[J].通化师范学院学报,2018,39(4):43-49.
[12]史磊,曹思思,吕邵娃,等.星点设计-效应面法优化马钱子碱-士的宁(B-S)双载药固体脂质纳米粒的制备工艺研究[J].中医药信息,2018,35(2):57-61.
[13]蔡晓瑶,张莉,任翔,等.离心法和透析法测定蟾皮提取物固体脂质纳米粒包封率的比较[J].国际药学研究杂志,2016,43(4):744-747.
[14]严春临,张季,刘敏,等.星点设计-效应面法优化吴茱萸次碱固体脂质纳米粒处方[J].中草药,2015,46(9):1307-1313.