一株冬虫夏草菌的鉴定及其抗氧化特性研究
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摘要
对分离自野生冬虫夏草(Cordyceps sinensis)的一株真菌F4539进行形态学和分子生物学鉴定,并对其发酵产物进行抗氧化活性研究。结果表明,F4539(Genbank号为KU359775)与Hirsutella sinensis(AJ309355)具有99%的相似性,初步确认F4539为中华被毛孢(Hirsutella sinensis)。抗氧化活性试验结果表明,F4539发酵物20 g/L乙醇提取液的DPPH抗氧化活性为75.39%,高于同浓度国产某商品化冬虫夏草菌菌丝体胶囊乙醇提取物的抗氧化活性,进一步研究表明,F4395发酵物和国产某商品清除DPPH自由基的IC50分别为5.96 g/L和11.61 g/L,即F4539发酵提取物清除DPPH自由基的能力强于国产某商品。
A fungus numbered F4539 was isolated from wild Cordyceps sinensis and identified by morphology and molecular biological identification, in addition, the antioxidant activity for the fermentation products were studied. The results demonstrated that F4539(Genbank: KU359775) had relatively high similarity in evolution with Hirsutella sinensis(AJ309355), it was confirmed that F4539 was Hirsutella sinensis; Antioxidant activity tests showed that the DPPH antioxidant activity was75.39% with a concentration of 20 g/L ethanol extract from F4539 fermentation products, which was higher than the same concentration of ethanol extracts from the certain commercial Cordyceps sinensis mycelium capsule. Further research showed that the IC50 of F4539 fermentation products and commercial Cordyceps sinensis mycelium capsule were 5.96 g/L and 11.61 g/L respectively. It was shown that the antioxidant activity was higher in F4539 fermentation products than the commercial Cordyceps sinensis mycelium capsule.
A fungus numbered F4539 was isolated from wild Cordyceps sinensis and identified by morphology and molecular biological identification, in addition, the antioxidant activity for the fermentation products were studied. The results demonstrated that F4539(Genbank: KU359775) had relatively high similarity in evolution with Hirsutella sinensis(AJ309355), it was confirmed that F4539 was Hirsutella sinensis; Antioxidant activity tests showed that the DPPH antioxidant activity was75.39% with a concentration of 20 g/L ethanol extract from F4539 fermentation products, which was higher than the same concentration of ethanol extracts from the certain commercial Cordyceps sinensis mycelium capsule. Further research showed that the IC50 of F4539 fermentation products and commercial Cordyceps sinensis mycelium capsule were 5.96 g/L and 11.61 g/L respectively. It was shown that the antioxidant activity was higher in F4539 fermentation products than the commercial Cordyceps sinensis mycelium capsule.
引文
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[2]LIU Z Q,LIN S,BAKER P J,et al.Transcriptome sequencing and analysis of the entomopathogenic fungus Hirsutella sinensis isolated from Ophiocordyceps sinensis[J].BMC Genomics,2015,16(1):106-123.
[3]LIU Z Y,LIANG Z Q,LIU A Y,et al.Molecular evidence for teleomorph-anamorph connections in Cordyceps based on ITS-5.8S rDNA sequences[J].Mycol Res,2002,106(9):1100-1108.
[4]CHEN Y Q,WANG N,QU L H,et al.Determination of the anamorph of Cordyceps sinensis inferred from the analysis of the ribosomal DNA internal transcribed spacers and 5.8S rDNA[J].Biochem Syst Ecol,2001,29(6):597-607.
[5]段琦梅,梁宗锁,聂小妮,等.黄芪和党参提取物的抗氧化活性研究[J].西北植物学报,2010,30(10):2123-2127.
[6]DONG C H,YAO Y J.Nutritional requirements of mycelial growth of Cordyceps sinensis in submerged culture[J].Journal of Applied Microbiology,2005,99(3):10.
[7]尹小武.冬虫夏草无性型的鉴定和生长特性研究[D].上海:上海交通大学,2009.
[8]赵聃聃,黄罗冬,索菲娅,等.冬虫夏草菌培养物抗氧化活性研究[J].中国食用菌,2015,34(1):65-69.
[9]姜微哲,渠凯,朱海波.冬虫夏草提取物调血脂与抗氧化活性[J].中国实验方剂学杂志,2011,17(12):127-131.