长链非编码RNA BC200促进食管鳞癌侵袭与转移
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摘要
目的研究长链非编码RNA BC200(LncRNA BC200,BC200)可否促进食管鳞癌细胞的侵袭与转移。方法应用q RT-PCR检测食管鳞癌细胞BC200的表达。应用划痕试验和Transwell检测抑制BC200表达后食管鳞癌细胞的迁移和侵袭能力的改变。应用MTT试验检测抑制BC200表达后食管鳞癌细胞增殖和凋亡的变化。结果高侵袭细胞系KYSE150中BC200的表达水平高于配对的低侵袭细胞系KYSE450,差异有统计学意义(t=4.125,P<0.01)。高侵袭细胞系KYSE410中BC200的表达水平高于配对的低侵袭细胞系KYSE510,差异有统计学意义(t=4.539,P<0.01)。高侵袭细胞系KYSE70中BC200的表达水平高于配对的低侵袭细胞系9706,差异有统计学意义(t=5.195,P<0.01)。稳定转染BC200 sh RNA的KYSE410细胞(KD组)在划痕4 h迁移率低于阴性病毒感染的KYSE410细胞(NC组),但差异无统计学意义(t=0.397,P>0.05); KD组在划痕8 h迁移率低于NC组,差异有统计学意义(t=2.586,P<0.05)。KD组培养24 h转移细胞变化倍数低于NC组,差异有统计学意义(t=4.298,P<0.05)。KD组存活细胞比例与对照组无明显变化,差异无统计学意义(P>0.05)。结论 BC200可促进食管鳞癌细胞侵袭与转移,抑制BC200表达后食管鳞癌细胞侵袭转移能力降低,增殖和凋亡能力无变化。
Objective To verify whether the long non-coding RNA(LncRNA BC200,BC200) can promote the invasion and metastasis of esophageal carcinoma cell lines.Methods The BC200 expression of esophageal squamous carcinoma cell lines was detected by q RT-PCR.Transwell and scratch test were applied to detect the changes of invasion and metastasis of esophageal squamous carcinoma cell lines after inhibition BC200.The changes of proliferation and apoptosis were detected by MTT test after inhibition BC200.Results The BC200 level in high invasion cell line KYSE150 was higher than that of the paired low invasion cell line KYSE450,and the difference was statistically significant(t=4.125,P<0.01).The BC200 level in high invasion cell line KYSE410 was higher than that of the paired low invasion cell line KYSE510,and the difference was statistically significant(t=4.539,P<0.01).The BC200 level in high invasion cell line KYSE150 was higher than that of the paired low invasion cell line KYSE450,and the difference was statistically significant(t=5.195,P<0.01).The migration rate of KYSE410 cells transfected with BC200 sh RNA(KD group) was lower than that of KYSE410 cells negative control group(NC group) at 4 hour,but the difference was no statistically significant(t=0.397,P>0.05).The migration rate of KD group was significantly lower than that of NC group at 8 hour,and the difference was statistically significant(t=2.586,P<0.05).The invasion fold in KD group was inferior to NC group at 24 hour,and the difference was statistically significant(t=4.298,P<0.05).Between KD group and NC group,surviving fraction had no obvious difference,and there was no statistically significant difference(P>0.05).Conclusion BC200 can promote invasion and metastasis of esophageal squamous carcinoma.With the knockdown of BC200,its ability of invasion and metastasis is decreased,and its ability of proliferation and apoptosis is no susceptible.
Objective To verify whether the long non-coding RNA(LncRNA BC200,BC200) can promote the invasion and metastasis of esophageal carcinoma cell lines.Methods The BC200 expression of esophageal squamous carcinoma cell lines was detected by q RT-PCR.Transwell and scratch test were applied to detect the changes of invasion and metastasis of esophageal squamous carcinoma cell lines after inhibition BC200.The changes of proliferation and apoptosis were detected by MTT test after inhibition BC200.Results The BC200 level in high invasion cell line KYSE150 was higher than that of the paired low invasion cell line KYSE450,and the difference was statistically significant(t=4.125,P<0.01).The BC200 level in high invasion cell line KYSE410 was higher than that of the paired low invasion cell line KYSE510,and the difference was statistically significant(t=4.539,P<0.01).The BC200 level in high invasion cell line KYSE150 was higher than that of the paired low invasion cell line KYSE450,and the difference was statistically significant(t=5.195,P<0.01).The migration rate of KYSE410 cells transfected with BC200 sh RNA(KD group) was lower than that of KYSE410 cells negative control group(NC group) at 4 hour,but the difference was no statistically significant(t=0.397,P>0.05).The migration rate of KD group was significantly lower than that of NC group at 8 hour,and the difference was statistically significant(t=2.586,P<0.05).The invasion fold in KD group was inferior to NC group at 24 hour,and the difference was statistically significant(t=4.298,P<0.05).Between KD group and NC group,surviving fraction had no obvious difference,and there was no statistically significant difference(P>0.05).Conclusion BC200 can promote invasion and metastasis of esophageal squamous carcinoma.With the knockdown of BC200,its ability of invasion and metastasis is decreased,and its ability of proliferation and apoptosis is no susceptible.
引文
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[17]Wu K,Xu K,Liu K,et al.Long noncoding RNA BC200 regulates cell growth and invasion in colon cancer[J].Int J Biochem Cell Biol,2018,99:219-225.
[18]Wu D I,Wang T,Ren C,et al.Downregulation of BC200 in ovarian cancer contributes to cancer cell proliferation and chemoresistance to carboplatin[J].Oncol Lett,2016,11(2):1189-1194.
[19]Booy E P,Mc Rae E K,Koul A,et al.The long non-coding RNA BC200(BCYRN1)is critical for cancer cell survival and proliferation[J].Mol Cancer,2017,16(1):109.
[20]Shin H,Lee J,Kim Y,et al.Knockdown of BC200 RNA expression reduces cell migration and invasion by destabilizing m RNA for calciumbinding protein S100A11[J].RNA Biol,2017,14(10):1418-1430.
[21]Jang S,Shin H,Lee J,et al.Regulation of BC200 RNA-mediated translation inhibition by hn RNP E1 and E2[J].FEBS Lett,2017,591(2):393-405.
[22]Grote P,Herrmann B G.The long non-coding RNA Fendrr links epigenetic control mechanisms to gene regulatory networks in mammalian embryogenesis[J].RNA Biol,2013,10(10):1579-1585.
[23]Hadjiargyrou M,Delihas N.The intertwining of transposable elements and non-coding RNAs[J].Int J Mol Sci,2013,14(7):13307-13328.
[24]Kretz M,Siprashvili Z,Chu C,et al.Control of somatic tissue differentiation by the long non-coding RNA TINCR[J].Nature,2013,493(7431):231-235.
[25]Xu C,Yang M,Tian J,et al.MALAT-1:a long non-coding RNA and its important 3'end functional motif in colorectal cancer metastasis[J].Int JOncol,2011,39(1):169-175.
[26]Tsai M C,Manor O,Wan Y,et al.Long noncoding RNA as modular scaffold of histone modification complexes[J].Science,2010,329(5992):689-693.
[27]Li T,Mo X,Fu L,et al.Molecular mechanisms of long noncoding RNAs on gastric cancer[J].Oncotarget,2016,7(8):8601-8612.
[28]Wang H,Li W,Guo R,et al.An intragenic long noncoding RNA interacts epigenetically with the RUNX1 promoter and enhancer chromatin DNA in hematopoietic malignancies[J].Int J Cancer,2014,135(12):2783-2794
[29]Mohammad F,Pandey G K,Mondal T,et al.Long noncoding RNA-mediated maintenance of DNA methylation and transcriptional gene silencing[J].Development,2012,139(15):2792-2803.
[2]Beermann J,Piccoli M T,Viereck J,et al.Non-coding RNAs in development and disease:background,mechanisms,and therapeutic approaches[J].Physiol Rev,2016,96(4):1297-1325.
[3]张丹,侯小丽,吴博,等.长链非编码RNA在食管癌中的研究进展[J].世界华人消化杂志,2015,(17):2744-2753.
[4]Zhao R H,Zhu C H,Li X K,et al.BC200 Lnc RNA a potential predictive marker of poor prognosis in esophageal squamous cell carcinoma patients[J].Onco Targets Ther,2016,9:2221-2226.
[5]Quan M,Chen J,Zhang D.Exploring the secrets of long noncoding RNAs[J].Int J Mol Sci,2015,16(3):5467-5496.
[6]Li J Y,Ma X,Zhang C B.Overexpression of long non-coding RNAUCA1 predicts a poor prognosis in patients with esophageal squamous cell carcinoma[J].Int J Clin Exp Pathol,2014,7(11):7938-7944.
[7]Wang X,Gao Z,Liao J,et al.lnc RNA UCA1 inhibits esophageal squamous-cell carcinoma growth by regulating the Wnt signaling pathway[J].J Toxicol Environ Health A,2016,79(9-10):407-418.
[8]Li X,Wu Z,Mei Q,et al.Long non-coding RNA HOTAIR,a driver of malignancy,predicts negative prognosis and exhibits oncogenic activity in oesophageal squamous cell carcinoma[J].Br J Cancer,2013,109(8):2266-2278.
[9]Chen F J,Sun M,Li S Q,et al.Upregulation of the long non-coding RNA HOTAIR promotes esophageal squamous cell carcinoma metastasis and poor prognosis[J].Mol Carcinog,2013,52(11):908-915.
[10]Lu J,Xie F,Geng L,et al.Investigation of serum lnc RNA-uc003wbd and lnc RNA-AF085935 expression profile in patients with hepatocellular carcinoma and HBV[J].Tumour Biol,2015,36(5):3231-3236.
[11]Tong Y S,Wang X W,Zhou X L,et al.Identification of the long noncoding RNA POU3F3 in plasma as a novel biomarker for diagnosis of esophageal squamous cell carcinoma[J].Mol Cancer,2015,14:3.
[12]Mus E,Hof P R,Tiedge H.Dendritic BC200 RNA in aging and in Alzheimer's disease[J].Proc Natl Acad Sci U S A,2007,104(25):10679-10684.
[13]Chen W,Bocker W,Brosius J,et al.Expression of neural BC200 RNAin human tumours[J].J Pathol,1997,183(3):345-351.
[14]Hu T,Lu Y R.BCYRN1,a c-MYC-activated long non-coding RNA,regulates cell metastasis of non-small-cell lung cancer[J].Cancer Cell Int,2015,15:36.
[15]Singh R,Gupta S C,Peng W X,et al.Regulation of alternative splicing of Bcl-x by BC200 contributes to breast cancer pathogenesis[J].Cell Death Dis,2016,7(6):e2262.
[16]Iacoangeli A,Lin Y,Morley E J,et al.BC200 RNA in invasive and preinvasive breast cancer[J].Carcinogenesis,2004,25(11):2125-2133.
[17]Wu K,Xu K,Liu K,et al.Long noncoding RNA BC200 regulates cell growth and invasion in colon cancer[J].Int J Biochem Cell Biol,2018,99:219-225.
[18]Wu D I,Wang T,Ren C,et al.Downregulation of BC200 in ovarian cancer contributes to cancer cell proliferation and chemoresistance to carboplatin[J].Oncol Lett,2016,11(2):1189-1194.
[19]Booy E P,Mc Rae E K,Koul A,et al.The long non-coding RNA BC200(BCYRN1)is critical for cancer cell survival and proliferation[J].Mol Cancer,2017,16(1):109.
[20]Shin H,Lee J,Kim Y,et al.Knockdown of BC200 RNA expression reduces cell migration and invasion by destabilizing m RNA for calciumbinding protein S100A11[J].RNA Biol,2017,14(10):1418-1430.
[21]Jang S,Shin H,Lee J,et al.Regulation of BC200 RNA-mediated translation inhibition by hn RNP E1 and E2[J].FEBS Lett,2017,591(2):393-405.
[22]Grote P,Herrmann B G.The long non-coding RNA Fendrr links epigenetic control mechanisms to gene regulatory networks in mammalian embryogenesis[J].RNA Biol,2013,10(10):1579-1585.
[23]Hadjiargyrou M,Delihas N.The intertwining of transposable elements and non-coding RNAs[J].Int J Mol Sci,2013,14(7):13307-13328.
[24]Kretz M,Siprashvili Z,Chu C,et al.Control of somatic tissue differentiation by the long non-coding RNA TINCR[J].Nature,2013,493(7431):231-235.
[25]Xu C,Yang M,Tian J,et al.MALAT-1:a long non-coding RNA and its important 3'end functional motif in colorectal cancer metastasis[J].Int JOncol,2011,39(1):169-175.
[26]Tsai M C,Manor O,Wan Y,et al.Long noncoding RNA as modular scaffold of histone modification complexes[J].Science,2010,329(5992):689-693.
[27]Li T,Mo X,Fu L,et al.Molecular mechanisms of long noncoding RNAs on gastric cancer[J].Oncotarget,2016,7(8):8601-8612.
[28]Wang H,Li W,Guo R,et al.An intragenic long noncoding RNA interacts epigenetically with the RUNX1 promoter and enhancer chromatin DNA in hematopoietic malignancies[J].Int J Cancer,2014,135(12):2783-2794
[29]Mohammad F,Pandey G K,Mondal T,et al.Long noncoding RNA-mediated maintenance of DNA methylation and transcriptional gene silencing[J].Development,2012,139(15):2792-2803.