组蛋白去甲基化酶JMJD3与UTX在Meckel's软骨发育过程中的表达
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摘要
目的研究组蛋白去甲基化酶JMJD3与UTX在Meckel's软骨发育过程中的表达。方法采用免疫组织化学方法观察小鼠胚胎期Meckel's软骨及其周围细胞凝聚区组蛋白去甲基化酶JMJD3与UTX的表达。结果 JMJD3与UTX均表达于细胞核内。JMJD3在软骨细胞内随着软骨细胞的发育成熟其表达由强变弱;UTX表达则是由弱变强。在Meckel's软骨周围细胞凝聚区,小鼠胚胎初期,JMJD3及UTX弱表达;细胞分化期JMJD3与UTX表达增强。结论 JMJD3及UTX参与Meckel's软骨细胞增殖、分化的发育过程,推测JMJD3及UTX参与下颌骨的发育。
Objective To examine the expression of JMJD3 and UTX in the mouse embryonic Meckel's cartilage.Method The levels of JMJD3 and UTX in different embryonic stages were determined by immunohistochemistry. Results JMJD3 and UTX expressed in the nucleus. JMJD3 declined during the development of the chondrocytes,but the expression of UTX showed the opposite. With the differentiation of those cells,the levels of JMJD3 and UTX increased. Conclusion JMJD3 and UTX may get involved in the development.
Objective To examine the expression of JMJD3 and UTX in the mouse embryonic Meckel's cartilage.Method The levels of JMJD3 and UTX in different embryonic stages were determined by immunohistochemistry. Results JMJD3 and UTX expressed in the nucleus. JMJD3 declined during the development of the chondrocytes,but the expression of UTX showed the opposite. With the differentiation of those cells,the levels of JMJD3 and UTX increased. Conclusion JMJD3 and UTX may get involved in the development.
引文
1 Bernstein BE,Mikkelsen TS,Xie X,et a1.A bivalent chromatin structure marks key developmental genes in embryonic stem cells.Cell,2006,125(2):315-326.
2 Lee TI,Jenner RG,Boyer LA,et al.Control of developmental regulators by Polycomb in human embryonic stem cells.Cell,2006,125(2):301-313.
3 Cao R,Wang L,Wang H.Role of histone H3 lysine 27 methylation in polycomb-group silencing.Science,2002,298(5595):1039-1043.
4 Hamada Y,Bando T,Nakamura T,et al.Leg regeneration is epigenetically regulatedby histone H3K27 methylation in the cricket Gryllus bimaculatus.Development,2015,142(17):2916-2927.
5 Qi HH,Sarkissian M,Hu GO,et al.Histone H4K20/H3K9demethylase PHF8 regulates zebrafish brain and craniofacial development.Nature,2010,466(7305):503-507.
6 Loenarz C,Ge W,Coleman ML,et al.PHF8,a gene associated with cleft lip/palate and Mental retardation,encodes for an Nepsilondimethyllysine demethylase.Hum Mol Genet,2010,19(2):217-222.
7 Macdonald ME,Hall BK.Altered timing of the extracellular-matrixmediated epithelial-mesenchmal interaction that initiates mandibular skeletogenesis in three inbred strain of mice:development,heterochrony,and evolutionary change in morphology.J Exp Zool,2001,291(3):258.
8 Chai Y,Bringas P Jr,Shuler C,et al.A mouse mandibular culture model permits the study of neural crest cell migration and tooth development.Int J Dev Biol,1998,42(1):87-94.
9 Tomo S,Ogita M,Tomo I.Development of mandibular cartilages in the rat.Anat Rec,1997,249(2):233-239.
10 李鑫,金岩.细胞凝聚区的形成在C57BL/6系小鼠下颌骨发育中的作用意义.实用口腔医学杂志,1999,15(1):45.
11 Zhang F,Xu LY,Xu LX.et al.JMJD3 promotes chondrocyte proliferation and hypertrophy during endochondral bone formation in mice.J Mol Cell Biol,2015,7(1):23-34.
12 冯欢,张超,张哲恺.H3K27me3去甲基化酶Jmjd3调节胎鼠肺上皮细胞增殖和分化.医学与临床,2015,35(9):1177-1181.
13 Shpargel KB,Sengoku T,Yokoyama S,et al.UTX and UTY demonstrate histone demethylase-independent function in mouse embryonic development.PLo S Gent,2012,8(9):e1002964.
14 Wang FS,Lian WS,Lee MS,et al.Histone demethylase UTX counteracts glucocorticoid Deregulation of osteogenesis by modulating histone-dependent and independent pathways.J Mol Med,2017,95(5):499-512.
15 Cho YW,Hong T,Hong S,et al.PTIP associates with MLL3-and MLL4containing histone H3 lysine 4 methytransferase complex.J Biol Chem,2007,282(28):20395-20406.
16 Herz HM,Mohan M,Garruss AS.et al.Enhancer-associated H3K4monomethylation by Trithorax-related,the Drosophila homolog of mammalian Mll3/Mll4.Genes Dev,2012,26(23):2604-2620.
2 Lee TI,Jenner RG,Boyer LA,et al.Control of developmental regulators by Polycomb in human embryonic stem cells.Cell,2006,125(2):301-313.
3 Cao R,Wang L,Wang H.Role of histone H3 lysine 27 methylation in polycomb-group silencing.Science,2002,298(5595):1039-1043.
4 Hamada Y,Bando T,Nakamura T,et al.Leg regeneration is epigenetically regulatedby histone H3K27 methylation in the cricket Gryllus bimaculatus.Development,2015,142(17):2916-2927.
5 Qi HH,Sarkissian M,Hu GO,et al.Histone H4K20/H3K9demethylase PHF8 regulates zebrafish brain and craniofacial development.Nature,2010,466(7305):503-507.
6 Loenarz C,Ge W,Coleman ML,et al.PHF8,a gene associated with cleft lip/palate and Mental retardation,encodes for an Nepsilondimethyllysine demethylase.Hum Mol Genet,2010,19(2):217-222.
7 Macdonald ME,Hall BK.Altered timing of the extracellular-matrixmediated epithelial-mesenchmal interaction that initiates mandibular skeletogenesis in three inbred strain of mice:development,heterochrony,and evolutionary change in morphology.J Exp Zool,2001,291(3):258.
8 Chai Y,Bringas P Jr,Shuler C,et al.A mouse mandibular culture model permits the study of neural crest cell migration and tooth development.Int J Dev Biol,1998,42(1):87-94.
9 Tomo S,Ogita M,Tomo I.Development of mandibular cartilages in the rat.Anat Rec,1997,249(2):233-239.
10 李鑫,金岩.细胞凝聚区的形成在C57BL/6系小鼠下颌骨发育中的作用意义.实用口腔医学杂志,1999,15(1):45.
11 Zhang F,Xu LY,Xu LX.et al.JMJD3 promotes chondrocyte proliferation and hypertrophy during endochondral bone formation in mice.J Mol Cell Biol,2015,7(1):23-34.
12 冯欢,张超,张哲恺.H3K27me3去甲基化酶Jmjd3调节胎鼠肺上皮细胞增殖和分化.医学与临床,2015,35(9):1177-1181.
13 Shpargel KB,Sengoku T,Yokoyama S,et al.UTX and UTY demonstrate histone demethylase-independent function in mouse embryonic development.PLo S Gent,2012,8(9):e1002964.
14 Wang FS,Lian WS,Lee MS,et al.Histone demethylase UTX counteracts glucocorticoid Deregulation of osteogenesis by modulating histone-dependent and independent pathways.J Mol Med,2017,95(5):499-512.
15 Cho YW,Hong T,Hong S,et al.PTIP associates with MLL3-and MLL4containing histone H3 lysine 4 methytransferase complex.J Biol Chem,2007,282(28):20395-20406.
16 Herz HM,Mohan M,Garruss AS.et al.Enhancer-associated H3K4monomethylation by Trithorax-related,the Drosophila homolog of mammalian Mll3/Mll4.Genes Dev,2012,26(23):2604-2620.