ECT2和P21蛋白在乳腺癌中的表达及意义
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摘要
目的探讨乳腺癌中ECT2和P21蛋白的表达及其临床意义。方法选取133例乳腺癌和80例正常乳腺组织,采用免疫组织化学En Vision的方法检测ECT2和P21蛋白在上述组织中的表达情况,分析二者在乳腺癌中的表达关系。结果ECT2蛋白在乳腺癌和正常组织中的阳性表达率分别为55.6%和8.8%,二者比较差异有统计学意义(P<0.05);ECT2与P21蛋白表达在患者不同年龄、不同肿瘤大小及不同肿瘤类型间比较差异无统计学意义(P>0.05),而在不同肿瘤分级、淋巴结转移与否及不同TNM分期间比较差异有统计学意义(P<0.05)。ECT2和P21蛋白在乳腺癌中的表达呈显著负相关(r=-0.242,P<0.05)。结论 ECT2和P21蛋白表达在乳腺癌的发生发展过程中均存在一定的作用,而且二者可能存在相互抑制作用。
Objective To explore the expression and clinical significance of ECT2 and P21 proteins in breast cancer. Methods Expression of ECT2 and P21 proteins was detected in 133 cases of breast cancer tissues and 80 cases of normal breast tissues using En Vision immunohistochemistry. Results The positive expression rates of ECT2 protein were 55. 6% and 8. 8% in breast cancer and normal breast tissues,respectively,and there was significant difference between the two groups( P < 0. 05). The positive expression rates of P21 proteins were 66. 9% and 12. 5% in breast cancer and normal breast tissues,respectively,and there was significant difference between the two groups( P < 0. 05). The expression of ECT2 and P21 protein was not correlated with age,tumor size,tumor type( P >0. 05),but correlated with tumor grade,lymphatic metastasis and TNM staging( P < 0. 05). ECT2 expression was negatively correlated with P21 expression in breast cancer( r =-0. 242,P < 0. 05). Conclusion The expression of ECT2 and P21 protein may play a role in the development of breast cancer,and inhibit each other.
Objective To explore the expression and clinical significance of ECT2 and P21 proteins in breast cancer. Methods Expression of ECT2 and P21 proteins was detected in 133 cases of breast cancer tissues and 80 cases of normal breast tissues using En Vision immunohistochemistry. Results The positive expression rates of ECT2 protein were 55. 6% and 8. 8% in breast cancer and normal breast tissues,respectively,and there was significant difference between the two groups( P < 0. 05). The positive expression rates of P21 proteins were 66. 9% and 12. 5% in breast cancer and normal breast tissues,respectively,and there was significant difference between the two groups( P < 0. 05). The expression of ECT2 and P21 protein was not correlated with age,tumor size,tumor type( P >0. 05),but correlated with tumor grade,lymphatic metastasis and TNM staging( P < 0. 05). ECT2 expression was negatively correlated with P21 expression in breast cancer( r =-0. 242,P < 0. 05). Conclusion The expression of ECT2 and P21 protein may play a role in the development of breast cancer,and inhibit each other.
引文
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[14]Han S,Woo JK,Jung Y,et al.Evodiamine selectively targets cancer stem-like cells through the p53-p21-Rb pathway[J].Biochem Biophys Res Commun,2016,469(4):1153-1158.
[2]Georgakilas AG,Martin OA,Bonner WM.p21:A two-faced genome guardian[J].Trends Mol Med,2017,23(4):310-319.
[3]Xu J1,Yao Q,Hou Y,et al.MiR-223/Ect2/p21 signaling regulates osteosarcoma cell cycle progression and proliferation[J].Biomed Pharmacother,2013,67(5):381-386.
[4]Iyoda M1,Kasamatsu A,Ishigami T,et al.Epithelial cell transforming sequence 2 in human oral cancer[J].PLo S One,2010,5(11):e14082.
[5]Chen J,Xia H,Zhang X,et al.ECT2 regulates the Rho/ERK signalling axis to promote early recurrence in human hepatocellular carcinoma[J].J Hepatol,2015,62(6):1287-1295.
[6]Luo Y,Qin SL,Mu YF,et al.Elevated expression of ECT2 predicts unfavorable prognosis in patients with colorectal cancer[J].Biomed Pharmacother,2015,73:135-139.
[7]Zhang H,Yin Z,Ning K,et al.Prognostic value of microRNA-223/epithelial cell transforming sequence 2 signaling in patients with osteosarcoma[J].Hum Pathol,2014,45(7):1430-1436.
[8]Wang LL,Guo HH,Zhan Y,et al.Specific up-regulation of p21by a small active RNA sequence suppresses human colorectal cancer growth[J].Oncotarget,2017,8(15):25055-25065.
[9]Chen Z,Wang K,Hou C,et al.CRL4BDCAF11 E3 ligase targets p21 for degradation to control cell cycle progression in human osteosarcoma cells[J].Sci Rep,2017,7(1):1175.
[10]Wang LL,Guo HH,Zhan Y,et al.Specific up-regulation of p21by a small active RNA sequence suppresses human colorectal cancer growth[J].Oncotarget,2017,8(15):25055-25065.
[11]Jiang X,Liu W.Long noncoding RNA highly upregulated in liver cancer activates p53-p21 pathway and promotes nasopharyngeal carcinoma cell growth[J].DNA Cell Biol,2017,36(7):596-602.
[12]Parsa Y,Mirmalek SA,Kani FE,et al.A review of the clinical implications of breast cancer biology[J].Electron Physician,2016,8(5):2416-2424.
[13]Wang LH,Jiang XR,Chen GL,et al.Anti-tumor activity of SL4against breast cancer cells:induction of G2/M arrest through modulation of the MAPK-dependent p21 signaling pathway[J].Sci Rep,2016,6:36486.
[14]Han S,Woo JK,Jung Y,et al.Evodiamine selectively targets cancer stem-like cells through the p53-p21-Rb pathway[J].Biochem Biophys Res Commun,2016,469(4):1153-1158.