结核患者外周血PBMC中T-bet和Eomes表达的检测
|
摘要
目的初步探讨T-bet和Eomes的表达与结核病发生的关系。方法分析比较结核患者(TB)与健康对照(HC)、结核菌潜伏感染者(LTBI)以及细菌性肺炎患者(PN)外周血PBMC中T-bet和Eomes的m RNA表达水平,并观察结核患者在抗结核治疗前及抗结核治疗3、6、9和12个月后,外周血PBMC中T-bet和Eomes的m RNA表达水平的变化。结果结核患者(TB)外周血PBMC中T-bet和Eomes的m RNA表达水平显著低于健康对照(HC)、结核菌潜伏感染者(LTBI)以及细菌性肺炎患者(PN)(P<0.05)。同时,在抗结核治疗12个月后,T-bet的表达水平较治疗前显著升高(P<0.05),Eomes的表达水平较治疗前升高,但差异无统计学意义(P>0.05)。结论 T-bet和Eomes两个基因表达水平的下降可能与结核病的发生有关。
Objective To study the relationship between the T-bet/ Eomes and the occurrence of tuberculosis.Methods Comparative analysis of expression of PBMC T-bet/Eomes m RNA in tuberculosis(TB),healthy controls(HC),latent tuberculosis infection(LTBI) and bacterial pneumonia(PN). Then,we observed the changes of T-bet/Eomes expressionbefore(0 m) and after 3 months,6 months,9 months and 12 months anti-tuberculosis therapy in the patients with tuberculosiscases. Results Expression of PBMC T-bet and Eomes in tuberculosis(TB) were significantly lower than those of healthycontrols(HC),latent tuberculosis infection(LTBI) and bacterial pneumonia(PN)(P<0.05). At the same time,the expressionlevel of T-bet was significantly increased after anti-tuberculosis treatment for 12 months(P<0.05),Eomes also increased butthere was no significant difference(P>0.05). Conclusion The decreased expression of T-bet and Eomes may be related tothe occurrence of tuberculosis..
Objective To study the relationship between the T-bet/ Eomes and the occurrence of tuberculosis.Methods Comparative analysis of expression of PBMC T-bet/Eomes m RNA in tuberculosis(TB),healthy controls(HC),latent tuberculosis infection(LTBI) and bacterial pneumonia(PN). Then,we observed the changes of T-bet/Eomes expressionbefore(0 m) and after 3 months,6 months,9 months and 12 months anti-tuberculosis therapy in the patients with tuberculosiscases. Results Expression of PBMC T-bet and Eomes in tuberculosis(TB) were significantly lower than those of healthycontrols(HC),latent tuberculosis infection(LTBI) and bacterial pneumonia(PN)(P<0.05). At the same time,the expressionlevel of T-bet was significantly increased after anti-tuberculosis treatment for 12 months(P<0.05),Eomes also increased butthere was no significant difference(P>0.05). Conclusion The decreased expression of T-bet and Eomes may be related tothe occurrence of tuberculosis..
引文
[1]WHO.Global tuberculosis report 2014[S].Geneva:World Health Organization,2014.
[2]陈效友.肺结核诊断的研究进展[J].中国临床医生,2013,41(3):11-14.
[3]INTLEKOFER A M,TAKEMOTO N,WHERRY E J,et al.Effector and memory CD8+T cell fate coupled by T-bet and eomesodermin[J].Nat Immunol,2006,6(12):1236-1244.
[4]SZABO S J,SULLIVAN B M,STEMMANN C,et al.Distinct effects of T-bet in TH1 lineage commitment and IFN-gamma production in CD4 and CD8 T cells[J].Science,2002,295(5553):338-342.
[5]TOWNSEND M J,WEINMANN A S,MATSUDA J L,et al.T-bet regulates the terminal maturation and homeostasis of NK and Valpha14i NKT cells[J].Immunity,2004,20(4):477-494.
[6]PEARCE E L,MULLEN A C,MARTINS G A,et al.Control of effector CD8+T cell function by the transcription factor Eomesodermin[J].Science,2003,302(5647):1041-1043.
[7]ZHU Y,JU S,CHEN E,et al.T-bet and eomesodermin are required for T cell-mediated antitumor immune responses[J].J Immunol,2010,185(6):3174-3183.
[8]王永生,杨倩婷,苟继周,等.颈淋巴结结核患者外周血和组织中T-bet表达的检测[J].中国热带医学,2014,14(5):513-515.
[9]LI G,YANG Q,ZHU Y,et al.T-Bet and Eomes Regulate the Balance between the effector/central memory T cells versus memory stem like T cells[J].PLo S One,2013,8(6):67401.
[10]TAKEMOTO N,INTLEKOFER A M,NORTHRUP J T,et al.Cutting Edge:IL-12 inversely regulates T-bet and eomesodermin expression during pathogen-induced CD8+T cell differentiation[J].J Immunol,2006,177(11):7515-7519.
[11]SULLIVAN B M,JOBE O,LAZAREVIC V,et al.Increased susceptibility of mice lacking T-bet to infection with Mycobacterium tuberculosis correlates with increased IL-10 and decreased IFN-gamma production[J].J Immunol,2005,175(7):4593-4602.
[2]陈效友.肺结核诊断的研究进展[J].中国临床医生,2013,41(3):11-14.
[3]INTLEKOFER A M,TAKEMOTO N,WHERRY E J,et al.Effector and memory CD8+T cell fate coupled by T-bet and eomesodermin[J].Nat Immunol,2006,6(12):1236-1244.
[4]SZABO S J,SULLIVAN B M,STEMMANN C,et al.Distinct effects of T-bet in TH1 lineage commitment and IFN-gamma production in CD4 and CD8 T cells[J].Science,2002,295(5553):338-342.
[5]TOWNSEND M J,WEINMANN A S,MATSUDA J L,et al.T-bet regulates the terminal maturation and homeostasis of NK and Valpha14i NKT cells[J].Immunity,2004,20(4):477-494.
[6]PEARCE E L,MULLEN A C,MARTINS G A,et al.Control of effector CD8+T cell function by the transcription factor Eomesodermin[J].Science,2003,302(5647):1041-1043.
[7]ZHU Y,JU S,CHEN E,et al.T-bet and eomesodermin are required for T cell-mediated antitumor immune responses[J].J Immunol,2010,185(6):3174-3183.
[8]王永生,杨倩婷,苟继周,等.颈淋巴结结核患者外周血和组织中T-bet表达的检测[J].中国热带医学,2014,14(5):513-515.
[9]LI G,YANG Q,ZHU Y,et al.T-Bet and Eomes Regulate the Balance between the effector/central memory T cells versus memory stem like T cells[J].PLo S One,2013,8(6):67401.
[10]TAKEMOTO N,INTLEKOFER A M,NORTHRUP J T,et al.Cutting Edge:IL-12 inversely regulates T-bet and eomesodermin expression during pathogen-induced CD8+T cell differentiation[J].J Immunol,2006,177(11):7515-7519.
[11]SULLIVAN B M,JOBE O,LAZAREVIC V,et al.Increased susceptibility of mice lacking T-bet to infection with Mycobacterium tuberculosis correlates with increased IL-10 and decreased IFN-gamma production[J].J Immunol,2005,175(7):4593-4602.