黄蜀葵花中5种黄酮类化合物对肠道L细胞AGEs/RAGE/p38MAPK/NF-κB信号通路的调节作用
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摘要
目的探讨黄蜀葵花中5种黄酮类化合物对肠道L细胞AGEs/RAGE/p38MAPK/NF-κB信号通路的调节作用。方法200 mg·L~(-1)AGEs作用肠道于L细胞株GLUTag细胞,Western blot检测细胞中RAGE、p22~(Phox)、p47~(Phox)、p53、Bax蛋白的相对表达量,ELISA法检测TNF-α、IL-1、IL-6、caspase-3、caspase-9的含量。实验分为7组:空白对照组(NG)、模型组(AGEs)、槲皮素组(QT)、异槲皮苷组(IQT)、金丝桃苷组(HY)、槲皮素-3'-O-葡萄糖苷组(QG)、棉皮素-8-O-葡萄糖醛酸苷组(GG)。NG细胞培养于DMEM低糖培养基中;AGEs细胞培养于含200 mg·L~(-1)AGEs的DMEM低糖培养基中;给药组细胞分别培养于含50μmol·L~(-1)各黄酮单体及含200 mg·L~(-1)AGEs的DMEM低糖培养基中。各组细胞经药物干预24 h后,Western blot检测细胞中RAGE、p22~(Phox)、p47~(Phox)、p53、Bax、p-p38MAPK、NF-κB蛋白的相对表达量,ELISA法检测TNF-α、IL-1、IL-6的含量及caspase-3、caspase-9的活性。结果模型组细胞中RAGE、p22~(Phox)、p47~(Phox)、Bax、caspase-3、caspase-9、Phospho-p38MAPK、NF-κB相对表达量及细胞上清液中TNF-α、IL-1、IL-6的分泌量明显上调(P<0.01),p53相对表达量明显下调(P<0.01)。50μmol·L~(-1)槲皮素、异槲皮苷、金丝桃苷、槲皮素-3'-O-葡萄糖苷、棉皮素-8-O-葡萄糖醛酸苷分别给药后,各蛋白的相对表达量及炎症因子的分泌量接近正常组。结论黄属葵花中5种黄酮类化合物能明显抑制肠道L细胞AGEs/RAGE/p38MAPK/NF-κB信号通路。
Aim To investigate the regulation of five flavonoids from Abelmoschl manihot on the AGEs/RAGE/p38MAPK/NF-κB signaling pathway in GLUTag cells. Methods With GLUTag cells treated with 200 mg · L~(-1)AGEs,Western blot analysis was applied to determine the expression of RAGE,p22 ~(Phox),p47~(Phox),p53 and Bax proteins and ELISA analysis was used to detect the levels of TNF-α, IL-1, IL-6,caspase-3,caspase-9. The cells were divided into seven groups: the normal group( NG), model group( AGEs),quercetin-treated group( QT),isoquercitrintreated group( IQT),hyperoside-treated group( HY),quercetin-3'-O-glucoside-treated group( QG),gossypetin-8-O-glucuronide-treated group( GG). NG cells were cultured in DMEM low-glucose culture medium without adding any other intervention factors; model group cells were cultured in DMEM low-glucose medium containing 200 mg·L~(-1)AGEs; cells in five treatment groups were cultured in DMEM low-glucose culture medium containing 200 mg · L~(-1)AGEs,which were respectively added 50 μmol·L~(-1)QT,IQT,HY,QG,GG. Western blot analysis was applied to determine the expression of RAGE,p22 ~(Phox),p47~(Phox),p53 and bax proteins and ELISA analysis was used to detect the levels of TNF-α,IL-1,IL-6,caspase-3,caspase-9. Results The contents of RAGE,p22 ~(Phox),p47~(Phox),bax,caspase-3,caspase-9,phospho-p38 MAPK,NF-κB and TNF-α,IL-1 and IL-6 significantly increased in model group cells( P < 0. 01),and the relative expression of p53 was significantly down-regulated( P <0. 01) in model group cells. After treated with 50μmol · L~(-1)quercetin, isoquercitrin, hyperoside,quercetin-3'-O-glucoside,gossypetin-8-O-glucuronide,the content of proteins and inflammatory factors were close to normal group. Conclusion The five flavonoids in Abelmoschus manihot( L.) Medicus can inhibit AGEs/RAGE/p38MAPK/NF-κB signaling pathway in GLUTag cells.
Aim To investigate the regulation of five flavonoids from Abelmoschl manihot on the AGEs/RAGE/p38MAPK/NF-κB signaling pathway in GLUTag cells. Methods With GLUTag cells treated with 200 mg · L~(-1)AGEs,Western blot analysis was applied to determine the expression of RAGE,p22 ~(Phox),p47~(Phox),p53 and Bax proteins and ELISA analysis was used to detect the levels of TNF-α, IL-1, IL-6,caspase-3,caspase-9. The cells were divided into seven groups: the normal group( NG), model group( AGEs),quercetin-treated group( QT),isoquercitrintreated group( IQT),hyperoside-treated group( HY),quercetin-3'-O-glucoside-treated group( QG),gossypetin-8-O-glucuronide-treated group( GG). NG cells were cultured in DMEM low-glucose culture medium without adding any other intervention factors; model group cells were cultured in DMEM low-glucose medium containing 200 mg·L~(-1)AGEs; cells in five treatment groups were cultured in DMEM low-glucose culture medium containing 200 mg · L~(-1)AGEs,which were respectively added 50 μmol·L~(-1)QT,IQT,HY,QG,GG. Western blot analysis was applied to determine the expression of RAGE,p22 ~(Phox),p47~(Phox),p53 and bax proteins and ELISA analysis was used to detect the levels of TNF-α,IL-1,IL-6,caspase-3,caspase-9. Results The contents of RAGE,p22 ~(Phox),p47~(Phox),bax,caspase-3,caspase-9,phospho-p38 MAPK,NF-κB and TNF-α,IL-1 and IL-6 significantly increased in model group cells( P < 0. 01),and the relative expression of p53 was significantly down-regulated( P <0. 01) in model group cells. After treated with 50μmol · L~(-1)quercetin, isoquercitrin, hyperoside,quercetin-3'-O-glucoside,gossypetin-8-O-glucuronide,the content of proteins and inflammatory factors were close to normal group. Conclusion The five flavonoids in Abelmoschus manihot( L.) Medicus can inhibit AGEs/RAGE/p38MAPK/NF-κB signaling pathway in GLUTag cells.
引文
[1]李庆林,王成永,彭代银,等.黄蜀葵花总黄酮对心肌缺血再灌注损伤的保护作用研究[J].中国实验方剂学杂志,2006,12(2):39-42.[1]Li Q L,Wang C Y,Peng D Y,et al.Protective effects of total flavone from the flowers of ablemoschl manihot L.Medic on the myocardial ischemia/reperfusion injury[J].Chin J Exp Tradit Med Formula,2006,12(2):39-42.
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[16]张利斌,张晓庆,李玉平,等.异槲皮苷抗抑郁作用实验研究[J].药学实践杂志,2011,29(4):272-5.[16]Zhang L B,Zhang X Q,Li Y P.Experimental study on the antidepressant activities of Isoquercitrin[J].J Pharm Pract,2011,29(4):272-5.
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[2]文继月,陈志武.黄蜀葵花总黄酮预处理对大鼠脑缺血再灌注性损伤的保护作用[J].安徽医科大学学报,2006,41(6):667-9.[2]Wen J Y,Chen Z W.Protective effect of pharmacological preconditioning of total flavones of Ablemoschl manihot L.Medic on cerebral ischemic reperfusion injury[J].Acta Univ Med Anhui,2006,41(6):667-9.
[3]陈萍,万毅刚,王朝俊,等.黄蜀葵花制剂治疗慢性肾脏病的机制和疗效[J].中国中药杂志,2012,37(15):2252-6.[3]Chen P,Wan Y G,Wang C J,et al.Mechanisms and effects of Abelmoschus manihot preparations in treating chronic kidney disease[J].China J Chin Mater Med,2012,37(15):2252-6.
[4]周晓隆,陈志武.黄蜀葵花总黄酮对实验性高血糖大鼠的作用及机制初探[J].中国临床药理学与治疗学,2016,21(6):618-20.[4]Zhou X L,Chen Z W.Effect of total flavones of abelmoschl manihot on rats with experimental hyperglycemia[J].Chin J Clin Pharmacol Ther,2016,21(6):618-20.
[5]潘武.黄蜀葵花总黄酮促血管新生作用及机制的实验研究[D].南京:南京中医药大学.2010.[5]Pan W.Experimental study on angiogenic effect and its mechalism of rotal flavone of ablemoschl manihot[D].Nanjing:Nanjing University of Chinese Medicine.2010.
[6]黄敬群,孙文娟,王四旺,等.槲皮素对大鼠痛风性关节炎抗炎抗氧化活性研究[J].中国实验方剂学杂志,2012,18(2):169-71.[6]Huang J Q,Sun W J,Wang S W,et al.Studies on the anti-inflammatory and antioxidant activity of quercetin in rats with gouty arthritis[J].Chin J Exp Tradit Med Formul,2012,18(2):169-71.
[7]胡庆华,缪明星,卢国,等.槲皮素对尿酸性肾病大鼠肾脏NLRP3和TLRs表达的影响[J].中草药,2013,44(24):3496-9.[7]Hu Q H,Miao M X,Lu G,et al.Effects of quercetin on expression of renal NLRP3 and TLRs in rats with uric acid nephtopathy[J].Chin Tradit Herbal Drugs,2013,44(24):3496-9.
[8]黄敬群,朱妙章,王四旺.染料木素、芹菜素、槲皮素、芦丁和落新妇苷对高尿酸血症小鼠黄嘌呤氧化酶活性及血清尿酸水平的影响[J].中国药理学通报,2011,27(4):561-5.[8]Huang J Q,Zhu M Z,Wang S W.Effects of Genistein,Apigenin,Quercetin,Rutin and Astibin on xanthine oxidase activities and serum uric acid levels in hyperuricemic mice[J].Chin Pharmacol Bull,2011,27(4):561-5.
[9]刘红亮,胡磊,王靖凯,等.槲皮素对H2O2损伤PC12细胞的保护效果与机制[J].中国药理学通报,2014,30(3):373-7.[9]Liu H L,Hu L,Wang J K,et al.Protective effects of quercetin on PC12 cells with H2O2-induced oxidative damage[J].Chin Pharmacol Bull,2014,30(3):373-7.
[10]施剑明,殷嫦嫦,孙维君,等.槲皮素联合顺铂对人骨肉瘤MG-63细胞增殖及凋亡的影响[J].中国药理学通报,2014,30(10):1361-6.[10]Shi J M,Yin C C,Sun W J,et al.Effect of quercetin combined with cisplatin on proliferation and apoptosis of human osteosarcoma cell line MG-63[J].Chin Pharmacol Bull,2014,30(10):1361-6.
[11]Choi J H,Kim D W,Yun N.Protective effects of hyperoside against carbon tetrachloride-induced liver damage in mice[J].J Nat Prod,2011,74(5):1055.
[12]李沛波,王永刚,吴钉红,等.田基黄中三个黄酮类化合物保肝退黄作用的实验研究[J].中山大学学报(医学科学版),2007,28(1):40-2.[12]Li P B,Wang Y G,Wu D H,et al.Experimental study of three flavonoids isolated from Hypericum japonicum Thunb.on Hepatoprotective and Jaundice-relieving Effects[J].J Sun Yat-sen Univ(Med Sci),2007,28(1):40-2.
[13]周晓隆,陈志武.金丝桃苷对新生大鼠神经细胞缺氧/再给氧损伤的保护作用及其机制[J].中国药理学通报,2010,26(1):83-6.[13]Zhou X L,Chen Z W.Action mechanism of hyperin on neonatal rats neuron with anoxia-reoxygenation[J].Chin Pharmacol Bull,2010,26(1):83-6.
[14]Haas J S,Stolz E D,Betti A H.The anti-immobility effect of hyperoside on the forced swimming test in rats is mediated by the D2-like receptors activation[J].Planta Med,2011,77(4):334.
[15]Kim S J,Um J Y,Lee J Y.Anti-inflammatory activity of hyperoside through the suppression of nuclear factor-κB activation in mouse peritoneal macrophages[J].Am J Chin Med,2011,39(1):171.
[16]张利斌,张晓庆,李玉平,等.异槲皮苷抗抑郁作用实验研究[J].药学实践杂志,2011,29(4):272-5.[16]Zhang L B,Zhang X Q,Li Y P.Experimental study on the antidepressant activities of Isoquercitrin[J].J Pharm Pract,2011,29(4):272-5.
[17]蔡红蝶,宿树兰,郭盛,等.黄蜀葵花中黄酮类成分对前脂肪细胞增殖、分化及胰岛素抵抗的影响[J].中国中药杂志,2016,41(24):4634-41.[17]Cai H D,Su S L,Guo S.Effect of flavonoids from Abelmoschus manihot(L.)Medic on proliferation,differentiation of 3T3-L1preadipocyte and insulin resistance[J].China J Chin Mater Med,2016,41(24):4634-41.
[18]Lim G E,Huang G J,Flora N,et al.Insulin regulates glucagonlike peptide-1 secretion from the enteroendocrine L cel I[J].Endocrinol,2009,150(2):580-91.
[19]Daoudi M,Hennuyer N,Borland M G,et al.PPARbeta/delta activation induces enteroendocrine L cell GLP-1 production[J].Gastroenterol,2011,140(5):1564-74.
[20]Hoist J J,Mcgill M A.Potential new approaches to modifying intestinal GLP-1 secretion in patients with type 2 diabetes mellitus:focus on bile acid sequestrants[J].Clin Drug Investig,2012,32(1):1-14.
[21]李双喜.AGEs-RAGE通路在2型糖尿病肠道L细胞早期损伤及凋亡中的机制研究[D].广州:南方医科大学,2014.[21]Li S X.The study on pathogenesis of AGEs-RAGE pathway in the early damageand apoptosis of intestinal L cells from T2DM[D].Guang zhou:Southern Medical University,2014.
[22]Dworakowski R,Alom-Ruiz S P,Shah A M.NADPH oxidase-derived reactive oxygen species in the regulation of endothelial phenotype[J].Pharmacol Rep,2008,60(1):21-8.
[23]Wang H J,Pan Y X,Wang W Z,et al.NADPH oxidase-derived reactive oxygen species in skeletal muscle modulates the exercise pressor reflex[J].J Appl Physiol(1985),2009,107(2):450-9.
[24]Wuyts W A,Vanaudenaerde B M,Dupont L J,et al.Involvement of p38 MAPK,JNK,p42/p44 ERK and NF-κB in IL-1β-induced chemokine release in human airway smooth muscle cells[J].Respiratory Med,2003,97(7):811-7.
[25]Lee J C,Kumar S,Griswold D E,et al.Inhibition of p38 MAP kinase as a therapeutic strategy[J].Immunopharmacol,2000,47(2-3):185-201.
[26]Wang X,Liu J Z,Hu J X,et al.ROS-activated p38 MAPK/ERK-Akt cascade plays a central role in palmitic acid-stimulated hepatocyte proliferation[J].Free Radical Biol Med,2011,51(2):539-51.
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