中华沙塘鳢(Odontobutis sinensis)精子入卵过程扫描电镜(SEM)观察
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摘要
为探讨中华沙塘鳢(Odontobutis sinensis)单精入卵机制,指导中华沙塘鳢人工繁育技术,采用扫描电子显微镜(SEM)对中华沙塘鳢精子和成熟卵子的形态和精子入卵过程予以观察,较为准确地描述了中华沙塘鳢精子和成熟卵细胞的形态与结构。结果显示,中华沙塘鳢精子头部为椭圆形,长轴约为2.25±0.25μm,短轴1.00±0.20μm,颈部较短。成熟卵细胞呈鲜艳黄色,呈圆球形,直径为1.30±0.02 mm,黏性卵,具黏着丝。观察发现,中华沙塘鳢精子能够准确通过成熟卵细胞的黏着丝达到受精孔,整个授精过程平均需要60 s完成,相对于其他科鱼类时间较长。中华沙塘鳢授精15 s,精子开始通过黏着丝;20 s大量精子进入筛网;25 s精子位于筛网孔内部,部分穿过筛网;55 s大量精子通过卵细胞黏着丝;60 s精子入卵;120 s受精孔关闭。
In order to explore the mechanism of single spermatozoa in Odontobutis sinensis,and to guide the artificial breeding,scanning electron microscopy(SEM) was used to observe the morphology and sperm entry process of spermatozoa and mature eggs of Odontobutis sinensis,and to accurately describe the morphology and structure of spermatozoa and mature egg cells in Odontobutis sinensis.The results showed that the head of sperm of the Odontobutis sinensis was elliptical,with a long axis of about 2.25±0.25 μm and a short axis of 1.00±0.20 μm,its neck was short.The mature egg cells are bright yellow,spherical,1.30±0.02 mm in diameter,sticky eggs with adhesive filaments.It was observed that the spermatozoon of the Odontobutis sinensis can accurately reach the fertilization hole through the adhesive filament of the mature egg cell,and the whole inse mination process takes an average of 60 s to complete,which is longer than other species.In the inse mination of Odontobutis sinensis,15 s,the sperm began to pass through the adhesion filament; 20 s large amount of sperm entered the cribriform column; 25 s sperm was located inside cribriform perforation(the hole of cribriform perforation),partially through cribriform perforation; 55 s a large number of sperm through the adhesion filament; 60 s sperm into the egg; 120 s fertilization hole shut down.
In order to explore the mechanism of single spermatozoa in Odontobutis sinensis,and to guide the artificial breeding,scanning electron microscopy(SEM) was used to observe the morphology and sperm entry process of spermatozoa and mature eggs of Odontobutis sinensis,and to accurately describe the morphology and structure of spermatozoa and mature egg cells in Odontobutis sinensis.The results showed that the head of sperm of the Odontobutis sinensis was elliptical,with a long axis of about 2.25±0.25 μm and a short axis of 1.00±0.20 μm,its neck was short.The mature egg cells are bright yellow,spherical,1.30±0.02 mm in diameter,sticky eggs with adhesive filaments.It was observed that the spermatozoon of the Odontobutis sinensis can accurately reach the fertilization hole through the adhesive filament of the mature egg cell,and the whole inse mination process takes an average of 60 s to complete,which is longer than other species.In the inse mination of Odontobutis sinensis,15 s,the sperm began to pass through the adhesion filament; 20 s large amount of sperm entered the cribriform column; 25 s sperm was located inside cribriform perforation(the hole of cribriform perforation),partially through cribriform perforation; 55 s a large number of sperm through the adhesion filament; 60 s sperm into the egg; 120 s fertilization hole shut down.
引文
[1] 伍汉霖,陈义雄.中国沙塘鳢属(Odontobutis)鱼类之一新种(鲈形目:沙塘鳢科)[J].上海海洋大学学报,2002,11(1):6-13.
[2] 伍汉霖,吴小清,解玉浩.中国沙塘鳢属鱼类的整理和一新种的叙述[J].上海海洋大学学报,1993(1):52-61.
[3] 刘良国,杨春英,杨品红,等.洞庭湖水系中华沙塘鳢的形态和核型研究[J].四川动物,2013,32(2):378-387.
[4] 曹晋飞.梁子湖中华沙塘鳢年龄生长、胚胎发育和消化道组织学的研究[D].华中农业大学,2014.56.
[5] 周剑,肖宇,李强,等.中华沙塘鳢在四川岷江汉阳水库发现[J].四川动物,2015,34(6):895.
[6] 李叶新,张应莲,裴成,等.中华沙塘鳢的人工繁殖技术[J].渔业致富指南,2017(1):35-38.
[7] 师瑞丹,吴志强,黄亮亮,等.湘江上游区桂北江段鱼类物种多样性研究[J].广西师范大学学报(自然科学版),2015,33(4):127-136.
[8] 王瑞霞,张毓人,傅仓生,等.鲂鱼受精早期精子入卵的扫描电子显微镜观察[J].水产学报,1982,6(4):313-320.
[9] 黄永松.尼罗罗非鱼成熟卵结构及精子入卵早期的电镜观察[J].动物学报,1990(3):227-230.
[10] 宋慧春,吴坤明,沈其璋,等.大银鱼卵膜孔结构的电镜观察[J]. 动物学报,1999,45(1):8-14.
[11] 何振邦,洪万树,陈仕玺,等.中华乌塘鳢精子入卵过程的扫描电镜观察[J].厦门大学学报(自然版),2009,48(1):128-133.
[12] Amanze D,Iyengar A.The micropyle:a sperm guidance system in teleost fertilization[J].DEVELOPMENT,1990,109(2):495-500.
[13] 潘光碧,邹世平.诱导鲤雌核发育时精子入卵的扫描电镜观察[J].中国水产科学,1999,6(3):28-31.
[14] Giulianini P G,Di Marcotullio A,Ferrero E A,et al.Light microscopical and ultrastructural cytology of the ovaries in the sea‐grass goby Zosterisessor ophiocephalus (Osteichthyes,Gobiidae) [J].Bolletino Di Zoologia,1994,61(2):135-144.
[15] 杨彩根,王永玲,宋学宏,等.沙塘鳢人工繁殖及鱼苗培育试验[J].水生态学杂志,2005,25(6):49.
[16] 杨长根,江志栋,宋长太,等.沙塘鳢人工繁殖与苗种培育技术试验[J].淡水渔业,2003,33(3):51-52.
[17] 杨长根,江志栋,宋长太,等.沙塘鳢人工繁殖与苗种培育技术[J].水产养殖,2003,24(2):19-20.
[18] 胡雨,高胜涛,刘亚秋,等.泥鳅精子和卵子结构及受精过程的细胞学观察[J].水生生物学报,2016(05):942-950.
[19] Giulianini P G,Ota D,Marchesan M,et al.Can goby spermatozoa pass through the filament adhesion apparatus of laid eggs[J]. J FISH BIOL,2010,58(6):1750-1752.
[20] Giulianini P G,Ferrero E A.Ultrastructural aspects of the ovarian follicle and egg envelope of the sea‐grass goby Zosterisessor ophiocephalus (Osteichthyes,Gobiidae) [J].ITAL J ZOOL,2001,68(1):29-37.
[2] 伍汉霖,吴小清,解玉浩.中国沙塘鳢属鱼类的整理和一新种的叙述[J].上海海洋大学学报,1993(1):52-61.
[3] 刘良国,杨春英,杨品红,等.洞庭湖水系中华沙塘鳢的形态和核型研究[J].四川动物,2013,32(2):378-387.
[4] 曹晋飞.梁子湖中华沙塘鳢年龄生长、胚胎发育和消化道组织学的研究[D].华中农业大学,2014.56.
[5] 周剑,肖宇,李强,等.中华沙塘鳢在四川岷江汉阳水库发现[J].四川动物,2015,34(6):895.
[6] 李叶新,张应莲,裴成,等.中华沙塘鳢的人工繁殖技术[J].渔业致富指南,2017(1):35-38.
[7] 师瑞丹,吴志强,黄亮亮,等.湘江上游区桂北江段鱼类物种多样性研究[J].广西师范大学学报(自然科学版),2015,33(4):127-136.
[8] 王瑞霞,张毓人,傅仓生,等.鲂鱼受精早期精子入卵的扫描电子显微镜观察[J].水产学报,1982,6(4):313-320.
[9] 黄永松.尼罗罗非鱼成熟卵结构及精子入卵早期的电镜观察[J].动物学报,1990(3):227-230.
[10] 宋慧春,吴坤明,沈其璋,等.大银鱼卵膜孔结构的电镜观察[J]. 动物学报,1999,45(1):8-14.
[11] 何振邦,洪万树,陈仕玺,等.中华乌塘鳢精子入卵过程的扫描电镜观察[J].厦门大学学报(自然版),2009,48(1):128-133.
[12] Amanze D,Iyengar A.The micropyle:a sperm guidance system in teleost fertilization[J].DEVELOPMENT,1990,109(2):495-500.
[13] 潘光碧,邹世平.诱导鲤雌核发育时精子入卵的扫描电镜观察[J].中国水产科学,1999,6(3):28-31.
[14] Giulianini P G,Di Marcotullio A,Ferrero E A,et al.Light microscopical and ultrastructural cytology of the ovaries in the sea‐grass goby Zosterisessor ophiocephalus (Osteichthyes,Gobiidae) [J].Bolletino Di Zoologia,1994,61(2):135-144.
[15] 杨彩根,王永玲,宋学宏,等.沙塘鳢人工繁殖及鱼苗培育试验[J].水生态学杂志,2005,25(6):49.
[16] 杨长根,江志栋,宋长太,等.沙塘鳢人工繁殖与苗种培育技术试验[J].淡水渔业,2003,33(3):51-52.
[17] 杨长根,江志栋,宋长太,等.沙塘鳢人工繁殖与苗种培育技术[J].水产养殖,2003,24(2):19-20.
[18] 胡雨,高胜涛,刘亚秋,等.泥鳅精子和卵子结构及受精过程的细胞学观察[J].水生生物学报,2016(05):942-950.
[19] Giulianini P G,Ota D,Marchesan M,et al.Can goby spermatozoa pass through the filament adhesion apparatus of laid eggs[J]. J FISH BIOL,2010,58(6):1750-1752.
[20] Giulianini P G,Ferrero E A.Ultrastructural aspects of the ovarian follicle and egg envelope of the sea‐grass goby Zosterisessor ophiocephalus (Osteichthyes,Gobiidae) [J].ITAL J ZOOL,2001,68(1):29-37.