巨型玫瑰冠鸡H-FABP、A-FABP基因表达的发育性变化及其肌内脂肪含量研究
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摘要
为探讨心脏型脂肪酸结合蛋白(heart-type fatty acid-binding protein,H-FABP)和脂肪型脂肪酸结合蛋白(adipocyte fatty acid-binding protein,A-FABP)基因与巨型玫瑰冠鸡生长发育及肌内脂肪含量(IMF)的关系,研究H-FABP、A-FABP基因对巨型玫瑰冠鸡IMF含量和腹脂沉积的作用机制,本试验采集了巨型玫瑰冠鸡与良凤花鸡在不同周龄(2、4、6、8、10、12周龄)的腹脂、心肌、胸肌、腿肌组织样品共480份,采用实时荧光定量PCR对巨型玫瑰冠鸡与良凤花鸡不同生长阶段组织中H-FABP、A-FABP基因mRNA表达量进行了检测,并采用索氏浸提法测定了两种鸡12周龄的胸肌与腿肌的IMF含量。结果表明,巨型玫瑰冠鸡与良凤花鸡的H-FABP、A-FABP基因mRNA在腹脂、心肌、胸肌和腿肌组织中均有不同程度的表达,且两种鸡H-FABP基因mRNA在心肌组织中高度表达,在脂肪组织中表达量较低,在胸肌、腿肌组织中中度表达,而A-FABP基因相反,推测H-FABP基因主要在肌肉组织中表达,而A-FABP基因主要在脂肪组织中表达。良凤花鸡生长速度高于巨型玫瑰冠鸡;巨型玫瑰冠鸡的腹脂率均低于同性别的良凤花鸡,但胸肌、腿肌的IMF均高于同性别的良凤花鸡,表明巨型玫瑰冠鸡的肉品质优于良凤花鸡。本试验结果为巨型玫瑰冠鸡H-FABP、A-FABP基因分子选育奠定了基础。
In order to investigate the relationship between heart-type fatty acid-binding protein(H-FABP)and adipocyte fatty acid-binding protein(A-FABP)genes and the growth and intramuscular fat(IMF)content of Rose cockscomb chicken,and the mechanism of H-FABP and AFABP genes effects on the IMF content and abdominal fat deposition,a total of 480 copies of the abdominal fat,myocardium,chest muscle and leg muscle samples of Rose cockscomb and Liangfenghua chickens were collected,the H-FABP and A-FABP genes mRNA expression were analyzed by Real-time quantitative PCR,and the IMF content of chest and leg muscles of 12-week-old chickens was determined by Soxhlet extraction method.The results showed that the H-FABP and A-FABP genes were all expressed in abdominal fat,myocardium,chest muscle and leg muscle tissues with varying degrees.The H-FABPgenes of Rose cockscomb and Liangfenghua chick-ens was highly expressed in myocardium tissues,moderately expressed in chest and leg muscles tissues,and rarely expressed in abdominal fat tissues.And the expression of A-FABPgene was on the contrary,indicating that H-FABP gene was mainly expressed in muscle tissues while AFABP gene was mainly expressed in adipose tissue.Liangfenghua chicken grew faster than Rose cockscomb chicken,the abdominal fat rat of Rose cockscomb chicken were lower than single-sex Liangfenghua chicken,but the IMF in chest muscle and leg muscle were higher than that of single-sex Liangfenghua chicken,showing that the meat quality of Rose cockscomb chicken was better.The test result laid a solid foundation for the H-FABPand A-FABP genes molecular breeding of Rose cockscomb chicken.
In order to investigate the relationship between heart-type fatty acid-binding protein(H-FABP)and adipocyte fatty acid-binding protein(A-FABP)genes and the growth and intramuscular fat(IMF)content of Rose cockscomb chicken,and the mechanism of H-FABP and AFABP genes effects on the IMF content and abdominal fat deposition,a total of 480 copies of the abdominal fat,myocardium,chest muscle and leg muscle samples of Rose cockscomb and Liangfenghua chickens were collected,the H-FABP and A-FABP genes mRNA expression were analyzed by Real-time quantitative PCR,and the IMF content of chest and leg muscles of 12-week-old chickens was determined by Soxhlet extraction method.The results showed that the H-FABP and A-FABP genes were all expressed in abdominal fat,myocardium,chest muscle and leg muscle tissues with varying degrees.The H-FABPgenes of Rose cockscomb and Liangfenghua chick-ens was highly expressed in myocardium tissues,moderately expressed in chest and leg muscles tissues,and rarely expressed in abdominal fat tissues.And the expression of A-FABPgene was on the contrary,indicating that H-FABP gene was mainly expressed in muscle tissues while AFABP gene was mainly expressed in adipose tissue.Liangfenghua chicken grew faster than Rose cockscomb chicken,the abdominal fat rat of Rose cockscomb chicken were lower than single-sex Liangfenghua chicken,but the IMF in chest muscle and leg muscle were higher than that of single-sex Liangfenghua chicken,showing that the meat quality of Rose cockscomb chicken was better.The test result laid a solid foundation for the H-FABPand A-FABP genes molecular breeding of Rose cockscomb chicken.
引文
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[13]陈锐,霍科科,张涛,等.略阳乌鸡与良凤花鸡肌肉肌苷酸含量比较[J].江苏农业科学,2013,41(8):195-197.CHEN R,HUO K K,ZHANG T,et al.Comparison of muscle inosinic acid content in Liaoyangwu chicken and Liangfeng chicken[J].Jiangsu Agricultural Sciences,2013,41(8):195-197.(in Chinese)
[14]游小燕,刘益平,朱庆,等.鸡H-FABP基因多态性及其与屠宰性能的关联分析[J].遗传,2007,29(2):230-234.YOU X Y,LIU Y P,ZHU Q,et al.Genetic polymorphism of chicken H-FABP gene and its association with slaughter performance[J].Hereditary,2007,29(2):230-234.(in Chinese)
[15]王启贵,关天竹,王守志,等.A-FABP基因多态性与肉鸡生长和体组成性状的关联[J].遗传,2011,33(2):153-162.WANG Q G,GUAN T Z,WANG S Z,et al.Association of A-FABP gene polymorphism with growth and body composition traits in broilers[J].Hereditary,2011,33(2):153-162.(in Chinese)
[16]常国斌,周琼,栾德琴,等.鸡A-FABP基因不同基因型遗传效应及初步验证[J].畜牧兽医学报,2014,42(8):1088-1094.CHANG G B,ZHOU Q,LUAN D Q,et al.Genetic effects and primary validation of different genotypes of chicken A-FABP genes[J].Chinese Journal of Animal and Veterinary Sciences,2014,42(8):1088-1094.(in Chinese)
[17]GERBENS F,VERBURG F J,VAN MOERKERK H T,et al.Association of heart and adipocyte fat acid binding protein gene expression with intramuscular fat content in pigs[J].Journal of Animal Science,2001,79:347-354.
[18]屠云洁,苏一军,王克华,等.利用实时荧光定量RTPCR检测鸡A-FABP和H-FABP基因的差异表达[J].中国畜牧杂志,2010,46(7):1-4.TU Y J,SU Y J,WANG K H,et al.Detection of differential expression of chicken A-FABP and HFABP genes by Real-time fluorescence quantitative RT-PCR[J].Chinese Journal of Animal Science,2010,46(7):1-4.(in Chinese)
[19]叶满红,文杰,曹红鹤,等.脂肪型脂肪酸结合蛋白基因多态性与鸡肉品质性状的关系研究[J].畜牧兽医学报,2007,38(6):526-532.YE M H,WEN J,CAO H H,et al.Study on the relationship between fatty acid-binding protein gene polymorphism and chicken quality traits[J].Chinese Journal of Animal and Veterinary Sciences,2007,38(6):526-532.(in Chinese)
[2]张成先,廖和荣,赵艳.3个鸡群体中4个微卫星DNA多态性及其与杂种优势的关系[J].中国畜牧兽医,2008,35(11):70-74.ZHANG C X,LIAO H R,ZHAO Y.Polymorphism of four microsatellite DNAs in three chicken populations and their relationship with heterosis[J].China Animal Husbandry&Veterinary Medicine,2008,35(11):70-74.(in Chinese)
[3]贾薇,张吉保,邵军,等.玫瑰冠鸡种质特性的研究[J].中国家禽,2014,36(13):5-9.JIA W,ZHANG J B,SHAO J,et al.Study on germplasm characteristics of rose[J].China Poultry,2014,36(13):5-9.(in Chinese)
[4]OCKNER R K,MANNING J A,POPPENHAUSEN R B,et al.Abinding protein for fatty acid in cytosol of intestinal mucosa,liver,myocardium and other tissues[J].Science,1972,177:56-58.
[5]王玉玲,丁淑芬,宋岩,等.索氏抽提法测定粗脂肪含量方法的探讨[J].粮食加工,2014,39(5):76-77.WANG Y L,DING S F,SONG Y,et al.DiscussingSoxhlet fat content determination method[J].Grain Processing,2014,39(5):76-77.(in Chinese)
[6]VEERKAMP J H,VAN MOERKERK H T.Fatty acid-binding protein and its relation to fatty acid oxidation[J].Molecular and Cellular Biochemistry,1993,123(1-2):101-106.
[7]向春和,范丽娜,耿鹏瑞,等.不同冠型鸡H-FABP基因单核苷酸多态性与脂肪性状的关联分析[J].西北农业学报,2012,21(9):1-5.XIANG C H,FAN L N,GENG P R,et al.Association of single nucleotide polymorphisms of H-FABP gene with different fat traits in different crowns[J].Acta Agriculturae Boreali-occidentalis Sinica,2012,21(9):1-5.(in Chinese)
[8]罗桂芬,陈继兰,孙世铎,等.鸡A-FABP基因多态性分析及其与脂肪性状的相关研究[J].遗传,2006,28(1):39-42.LUO G F,CHEN J L,SUN S J,et al.Analysis of chicken A-FABP gene polymorphism and its correlation with fat traits[J].Hereditary,2006,28(1):39-42.(in Chinese)
[9]任文仕,朱庆,杨琴,等.不同品系鸡肌内脂肪和肌苷酸含量比较分析[J].中国畜牧兽医,2011,38(6):230-232.REN W S,ZHU Q,YANG Q,et al.Comparative analysis of intramuscular fat andinosinic acid content in different strains of chicken[J].China Animal Husbandry&Veterinary Medicine,2011,38(6):230-232.(in Chinese)
[10]王彦,朱庆,舒鼎铭,等.鸡H-FABP基因多态性及其与肌内脂肪含量的相关研究[J].中国畜牧杂志,2007,43(11):1-5.WANG Y,ZHU Q,SHU D M,et al.Chicken HFABP gene polymorphism and its correlation with intramuscular fat content[J].Chinese Journal of Animal Science,2007,43(11):1-5.(in Chinese)
[11]周琼,常国斌,王克华,等.如皋鸡EX-FABP基因遗传多态性及其与肌内脂肪含量的相关分析[J].中国畜牧兽医,2010,37(4):143-145.ZHOU Q,CHANG G B,WANG K H,et al.Genetic polymorphism of EX-FABP gene in Ruogao chicken and its correlation with intramuscular fat content[J].China Animal Husbandry&Veterinary Medicine,2010,37(4):143-145.(in Chinese)
[12]邵勇钢,臧长江,曾伟丹,等.拜城油鸡公鸡A-FABP基因多态性及其与生产性能的相关性分析[J].中国畜牧兽医,2017,44(1):141-147.SHAO Y G,ZANG C J,ZENG W D,et al.Polymorphism of A-FABP gene and its correlation with productive performance in male Baicheng fatty chicken[J].China Animal Husbandry&Veterinary Medicine,2017,44(1):141-147.(in Chinese)
[13]陈锐,霍科科,张涛,等.略阳乌鸡与良凤花鸡肌肉肌苷酸含量比较[J].江苏农业科学,2013,41(8):195-197.CHEN R,HUO K K,ZHANG T,et al.Comparison of muscle inosinic acid content in Liaoyangwu chicken and Liangfeng chicken[J].Jiangsu Agricultural Sciences,2013,41(8):195-197.(in Chinese)
[14]游小燕,刘益平,朱庆,等.鸡H-FABP基因多态性及其与屠宰性能的关联分析[J].遗传,2007,29(2):230-234.YOU X Y,LIU Y P,ZHU Q,et al.Genetic polymorphism of chicken H-FABP gene and its association with slaughter performance[J].Hereditary,2007,29(2):230-234.(in Chinese)
[15]王启贵,关天竹,王守志,等.A-FABP基因多态性与肉鸡生长和体组成性状的关联[J].遗传,2011,33(2):153-162.WANG Q G,GUAN T Z,WANG S Z,et al.Association of A-FABP gene polymorphism with growth and body composition traits in broilers[J].Hereditary,2011,33(2):153-162.(in Chinese)
[16]常国斌,周琼,栾德琴,等.鸡A-FABP基因不同基因型遗传效应及初步验证[J].畜牧兽医学报,2014,42(8):1088-1094.CHANG G B,ZHOU Q,LUAN D Q,et al.Genetic effects and primary validation of different genotypes of chicken A-FABP genes[J].Chinese Journal of Animal and Veterinary Sciences,2014,42(8):1088-1094.(in Chinese)
[17]GERBENS F,VERBURG F J,VAN MOERKERK H T,et al.Association of heart and adipocyte fat acid binding protein gene expression with intramuscular fat content in pigs[J].Journal of Animal Science,2001,79:347-354.
[18]屠云洁,苏一军,王克华,等.利用实时荧光定量RTPCR检测鸡A-FABP和H-FABP基因的差异表达[J].中国畜牧杂志,2010,46(7):1-4.TU Y J,SU Y J,WANG K H,et al.Detection of differential expression of chicken A-FABP and HFABP genes by Real-time fluorescence quantitative RT-PCR[J].Chinese Journal of Animal Science,2010,46(7):1-4.(in Chinese)
[19]叶满红,文杰,曹红鹤,等.脂肪型脂肪酸结合蛋白基因多态性与鸡肉品质性状的关系研究[J].畜牧兽医学报,2007,38(6):526-532.YE M H,WEN J,CAO H H,et al.Study on the relationship between fatty acid-binding protein gene polymorphism and chicken quality traits[J].Chinese Journal of Animal and Veterinary Sciences,2007,38(6):526-532.(in Chinese)