FADS2基因rs174575多态性与乳汁多不饱和脂肪酸水平的关系研究
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摘要
目的探讨Δ6去饱和酶基因(FADS2)rs174575位点基因多态性对乳汁多不饱和脂肪酸(PUFA)水平的影响。方法采用3天24 h膳食回顾调查乳母膳食PUFA摄入情况;于乳母产后22~25天收取成熟乳20 ml,气相色谱仪检测乳汁8种PUFA水平,试剂盒提取乳汁基因组DNA,并运用Sequenom mass array平台检测FADS2基因rs174575位点基因多态性。结果rs174575位点主等位基因C频率为90.2%,次等位基因G频率为9.8%;主等位基因纯合子CC携带者乳汁a-亚麻酸(ALA)低于G等位基因携带者ALA水平;而主等位基因纯合子CC携带者n-6与n-3系PUFA比值大于G等位基因携带者比值。结论 FADS2 rs174575基因多态性与乳母乳汁ALA水平及n-6/n-3比值水平相关联。
Objective:To explore the effect of rs174575 polymorphism of FADS2 gene on polyunsaturated fatty acids(PUFA) levels in breast milk.Methods:PUFA intake in breast milk was surveyed retrospectively by 24-hour dietary recall method for three days;20 ml mature milk specimens were collected at 22-25 days after delivery; gas chromatograph was used to detect the levels of eight kinds of PUFA; DNA extraction kit was used to abstract genomic DNA from breast milk; Sequenom mass array was used to detect rs174575 polymorphism of FADS2 gene.Results:The frequencies of the major allele(C) and the minor allele(G) of rs174575 locus were 90.2% and9.8%,respectively.Major allele homozygote(CC) carriers had lower level of a-linolenic acid(ALA) compared with allele G carriers; PUFA ratio of n-6 to n-3 in major allele homozygote(CC) carriers was higher than that in allele G carriers.Conclusion:rs174575 polymorphism of FADS2 gene is correlated with ALA level in breast milk and the ratio of n-6 to n-3.
Objective:To explore the effect of rs174575 polymorphism of FADS2 gene on polyunsaturated fatty acids(PUFA) levels in breast milk.Methods:PUFA intake in breast milk was surveyed retrospectively by 24-hour dietary recall method for three days;20 ml mature milk specimens were collected at 22-25 days after delivery; gas chromatograph was used to detect the levels of eight kinds of PUFA; DNA extraction kit was used to abstract genomic DNA from breast milk; Sequenom mass array was used to detect rs174575 polymorphism of FADS2 gene.Results:The frequencies of the major allele(C) and the minor allele(G) of rs174575 locus were 90.2% and9.8%,respectively.Major allele homozygote(CC) carriers had lower level of a-linolenic acid(ALA) compared with allele G carriers; PUFA ratio of n-6 to n-3 in major allele homozygote(CC) carriers was higher than that in allele G carriers.Conclusion:rs174575 polymorphism of FADS2 gene is correlated with ALA level in breast milk and the ratio of n-6 to n-3.
引文
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8 Xie L,Innis SM.Genetic variants of the FADS1 FADS2 gene cluster are associated with altered(n-6)and(n-3)essential fatty acids in plasma and erythrocyte phospholipids in women during pregnancy and in breast milk during lactation[J].The Journal of nutrition,2008;138(11):2222-2228.
9 Harslof LB,Larsen LH,Ritz C,et al.FADS genotype and diet are important determinants of DHA status:a cross-sectional study in Danish infants[J].The American journal of clinical nutrition,2013,97(6):1403-1410.
10 Steer CD,Hibbeln JR.Golding J,et al.Polyunsaturated fatty acid levels in blood during pregnancy,at birth and at 7 years:their associations with two common FADS2 polymorphisms[J].Human Molecular Genetics,2012,21(7):1504-1512.
2 Feart C,Peuchant E,Letenneur L,et al.Plasma eicosapentaenoic acid is inversely associated with severity of depressive symptomatology in the elderly:data from the Bordeaux sample of the Three-City Study[J].The American journal of clinical nutrition,2008,87(5):1156-1162.
3 Rzehak P,Heinrich J,Klopp N,et al.Evidence for an association between genetic variants of the fatty acid desaturase 1 fatty acid desaturase 2(FADS1 FADS2)gene cluster and the fatty acid composition of erythrocyte membranes[J].Br J Nutr,2009,101(1):20-26.
4 Schaeffer L,Gohlke H,Muller M,et al.Common genetic variants of the FADS1 FADS2 gene cluster and their reconstructed haplotypes are associated with the fatty acid composition in phospholipids[J].Hum Mol Genet,2006,15(11):1745-1756.
5 Molto-Puigmarti C,Plat J,Mensink RP,et al.FADS1 FADS2gene variants modify the association between fish intake and the docosahexaenoic acid proportions in human milk[J].The American journal of clinical nutrition,2010,91(5):1368-1376.
6 中国营养学会,编著.中国居民膳食营养素参考摄入量[M].北京科学出版社,2013:133-135.
7 杨镜玉,甘振威,张娅婕,等.乳汁提取基因组DNA及基因多态性检测研究[J].中国妇幼保健,2011,26(29):4594-4595.
8 Xie L,Innis SM.Genetic variants of the FADS1 FADS2 gene cluster are associated with altered(n-6)and(n-3)essential fatty acids in plasma and erythrocyte phospholipids in women during pregnancy and in breast milk during lactation[J].The Journal of nutrition,2008;138(11):2222-2228.
9 Harslof LB,Larsen LH,Ritz C,et al.FADS genotype and diet are important determinants of DHA status:a cross-sectional study in Danish infants[J].The American journal of clinical nutrition,2013,97(6):1403-1410.
10 Steer CD,Hibbeln JR.Golding J,et al.Polyunsaturated fatty acid levels in blood during pregnancy,at birth and at 7 years:their associations with two common FADS2 polymorphisms[J].Human Molecular Genetics,2012,21(7):1504-1512.