紫外-可见分光光度法在定量监测2,3,5-三碘苯甲酸缓释中的应用
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摘要
为研究显影剂2,3,5-三碘苯甲酸(TIBA)在显影纤维聚对二氧环己酮(PPDO)降解液中的缓释规律,采用紫外可见分光光度法探究了TIBA在不同体积比的磷酸盐缓冲溶液(PBS)和乙醇共混降解液(V_(PBS)∶V_(乙醇)分别为1∶3、1∶1和3∶1)中的紫外-可见分光光谱和回归曲线,根据回归曲线,测定降解液的吸光度值及计算PPDO纤维在降解过程中显影剂TIBA的释放量,借助药物缓释理论模型进行拟合,并分析相应的释放规律。结果表明:在3种共混溶液中,均能得到回归关系明显的线性方程,考虑到乙醇用量,选择PBS与乙醇的体积比为3∶1的溶液作为标准液,并且溶液在37℃的环境中静置8 d后仍能保持一定的稳定性;模型拟合结果表明TIBA的释放规律符合一级释放的函数模型。
In order to study the sustained release rules of 2,3,5-triiodobenzoic acid(TIBA) in degradation solution of radiopaque poly-p-dioxanone(PPDO) filaments, the UV spectrums and regression curves of TIBA in different volume ratio blend degradation solutions of phosphate buffered saline(PBS) and ethanol(V_(PBS)∶V_(ethanol) were 1∶3,1∶1 and 3∶1) were explored by UV-Vis Spectrophotometry. According to regression curve, the release amount of TIBA in the degradation period was measured and calculated and then fitted with a drug release models and the corresponding release rules were analyzed. The results show that ideal spectrums and regression curves could be obtained in three kinds of blend solution. Considering the amount of ethanol used, the volume ratio of 3∶1 was selected as the standard solution and it can still maintain stability after placing in an environment of 37 ℃ for 8 d. The results of model fit show that the release rule of TIBA is in consistence with the first-order release model.
In order to study the sustained release rules of 2,3,5-triiodobenzoic acid(TIBA) in degradation solution of radiopaque poly-p-dioxanone(PPDO) filaments, the UV spectrums and regression curves of TIBA in different volume ratio blend degradation solutions of phosphate buffered saline(PBS) and ethanol(V_(PBS)∶V_(ethanol) were 1∶3,1∶1 and 3∶1) were explored by UV-Vis Spectrophotometry. According to regression curve, the release amount of TIBA in the degradation period was measured and calculated and then fitted with a drug release models and the corresponding release rules were analyzed. The results show that ideal spectrums and regression curves could be obtained in three kinds of blend solution. Considering the amount of ethanol used, the volume ratio of 3∶1 was selected as the standard solution and it can still maintain stability after placing in an environment of 37 ℃ for 8 d. The results of model fit show that the release rule of TIBA is in consistence with the first-order release model.
引文
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[2] ZHAO F, XUE W, WANG F J, et al. A new approach to improve the local compressive properties of PPDO self-expandable stent[J]. Journal of the Mechanical Behavior of Biomedical Materials, 2017, 68: 318-326.
[3] ZHAO Y Q, DING S D, YUAN Y, et al. Enhanced degradation stability of poly(p-dioxanone) under different temperature and humidity with bis-(2,6-diisopropylphenyl) carbodiimide[J]. Journal of Applied Polymer Science, 2014, 131(6): 596-602.
[4] 李利发, 张井潇, 陈小波, 等.三种不同缝合线对腹壁手术切口愈合质量影响的研究[J].中国普外基础与临床杂志, 2016,23(4):445-449.LI Lifa, ZHANG Jingxiao, CHEN Xiaobo, et al. Study on the effect of three kinds of different suture on the healing quality of abdominal incision[J]. Chinese Journal of Bases and Clinics in General Surgery, 2016,23(4):445-449.
[5] ZHAO F, XUE W, WANG F J, et al. Composite self-expanding bioresorbable prototype stents with reinforced compression performance for congenital heart disease application: computational and experimental investigation[J]. Journal of the Mechanical Behavior of Biomedical Materials, 2018,84:126-134.
[6] TANG H, XU Z, QIN X, et al.Chest wall reconstruction in a canine model using polydioxanone mesh, demineralized bone matrix and bone marrow stromal cells[J]. Biomaterials, 2009, 30(19): 3224-3233.
[7] SINGHANA B, CHEN A, SLATTERY P, et al. Infusion of iodine-based contrast agents into poly(p-dioxanone) as a radiopaque resorbable IVC filter[J]. Journal of Materials Science: Materials in Medicine, 2015, 26(3): 124.
[8] 曲乐,曹江,黄新苗.可降解聚对二氧环己酮材料的临床应用[J].中国组织工程研究与临床康复, 2011,15(3): 527-530.QU Le, CAO Jiang, HUANG Xinmiao. Clinical application of biodegradable polydioxanone[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011,15(3): 527-530.
[9] SERIO P, FAINARDI V, LEONE R, et al. Tracheobronchial obstruction: follow-up study of 100 children treated with airway stenting[J]. European Journal of Cardio-Thoracic Surgery, 2014, 45(4): 100-109.
[10] LEON C M, LEE B H, PREUL M, et al. Synthesis and characterization of radio-opaque thermosensitive poly N-isopropylacrylamide-2,2′-(ethylenedioxy)bis(ethylamine)-2,3,5-triiodobenzamide[J].Polymer International,2009,58(8):847-850.
[11] STURZU A, VOGEL U, GHARABAGHI A, et al. Cell nucleus directed 2,3,5-triiodobenzoic acid conju-gates[J]. Medicinal Chemistry, 2009,5(4):385-391.
[12] MARTYNENKO A, CHEN Y. Degradation kinetics of total anthocyanins and formation of polymeric color in blueberry hydrothermodynamic (HTD) processing[J]. Journal of Food Engineering, 2016, 171(4):44-51.
[13] 陈力, 刘砚韬, 黄亮, 等. 缓控释系统药物释放的数学模型研究进展[J].中国药业,2008,17(11):1-4.CHEN Li, LIU Yantao, HUANG Liang, et al. Research progress of mathematic model of sustained and controlled release system of drug release[J]. China Pharmaceuticals, 2008,17(11):1-4.
[14] TABANDEH H, MORTAZAVI S A. An investigation into the drug release from ibuprofen matrix tablets with ethylcellulose and some poly-acrylate polymers[J]. Pakistan Journal of Pharmaceutical Sciences, 2014, 27(3):495-503.
[15] KAFFASHI B, DAVOODI S, OLIAEI E. Poly(epsilon-caprolactone)/triclosan loaded polylactic acid nanoparticles composite: a long-term antibacterial bionanocomposite with sustained release[J]. International Journal of Pharmaceutics, 2016, 508(1/2):10-21.
[16] KARAMI Z, REZAEIAN I, ZAHEDI P, et al. Preparation and performance evaluations of electrospun poly(epsilon-caprolactone), poly(lactic acid), and their hybrid (50/50) nanofibrous mats containing thymol as an herbal drug for effective wound healing[J]. Journal of Applied Polymer Science,2013,129(2):756-766.
[17] 钟萌,谭群友,冯悦,等.阿奇霉素囊泡体外释放行为的初步研究[J].中国抗生素杂志, 2012, 37(6):476-480.ZHONG Meng, TAN Qunyou, FENG Yue, et al.Preliminary study on the release of azithromycin niosomes in vitro[J]. Chinese Journal of Antibiotics, 2012, 37(6):476-480.