转fat-1基因动物模型和n-3 PUFAs功能作用机理研究进展
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摘要
研究fat-1基因功能的作用机理对充分合理利用多不饱和脂肪酸(PUFAs)资源具有重要意义,建立转fat-1基因动物模型是了解fat-1基因功能、作用机理的重要方法。文章详细阐述了fat-1基因通过n-3 PUFAs而发挥的生理功能、转fat-1基因动物神经系统疾病模型、转fat-1基因动物肿瘤疾病模型、转fat-1基因炎症动物疾病模型及作用机理研究进展,并对转fat-1基因动物抗肥胖作用机理、转fat-1基因动物在优质家畜育种改良中的作用、利用转fat-1基因动物模型研究骨骼肌发育机理和糖尿病的治疗方法等方面的进展进行了总结,以期为合理利用PUFAs资源和优质家畜改良育种提供理论依据。
The study of the functional mechanism of fat-1 gene is very important to the full rational utilization of unsaturated fatty acid resources,the establishment of fat-1 transgenic animal model is an important way to understand the mechanism of n-3 PUFAs function. The article describes in detail the physiological function of the fat-1 gene, the animal nervous system disease model of the transfat-1 gene, the animal tumor disease model of the transfat-1 gene, the animal disease model of the transfat-1 gene inflammation, and the progress in research on the mechanism of action.The mechanism of anti-obesity action of transfat-1 gene animals, the role of transfat-1 gene animals in breeding improvement of high-quality livestock, the mechanism of skeletal muscle development using transfat-1 gene animal model and the treatment of diabetes were summarized to provide a theoretical basis for the rational use of PUFAs resources and high-quality livestock breeding improvement.
The study of the functional mechanism of fat-1 gene is very important to the full rational utilization of unsaturated fatty acid resources,the establishment of fat-1 transgenic animal model is an important way to understand the mechanism of n-3 PUFAs function. The article describes in detail the physiological function of the fat-1 gene, the animal nervous system disease model of the transfat-1 gene, the animal tumor disease model of the transfat-1 gene, the animal disease model of the transfat-1 gene inflammation, and the progress in research on the mechanism of action.The mechanism of anti-obesity action of transfat-1 gene animals, the role of transfat-1 gene animals in breeding improvement of high-quality livestock, the mechanism of skeletal muscle development using transfat-1 gene animal model and the treatment of diabetes were summarized to provide a theoretical basis for the rational use of PUFAs resources and high-quality livestock breeding improvement.
引文
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[23] LIU X X,PANG D X,YUAN T,et al.N-3 polyunsaturated fatty acids attenuates triglyceride and inflammatory factors level in hfat-1 transgenic pigs[J].Lipids Health Dis,2016,15:89.
[24] 周飞,张晓宏,邹祖全,等.内源性n-3多不饱和脂肪酸对fat-1转基因小鼠血糖血脂的影响[J].宁波大学学报(理工版),2015,28(3):98-102.
[25] CHILTON F H,MURPHY R C,WILSON B A,et al. Diet-gene interactions and PUFA metabolism: a potential contributor to health disparities and human diseases[J]. Nutrients,2014, 6(5):1993-2022.
[26] FENTON J I,HORD N G,GHOSH S,et al. Immunomodulation by dietary long chain omega-3 fatty acid immunomodulation and the potential for adverse health outcomes[J].Prostaglandins Leukot Essent Fatty Acids,2013,89(6):379-390.
[2] KANG J X, WANG J, WU L,et al.Transgenic mice: fat-1 mice convert n-6 to n-3 fatty acids[J].Nature,2004,427(6974):504.
[3] SIMOPOULOS A P.The importance of the omega-6/omega-3 fatty acid ratio in cardiovascular disease and other chronic diseases[J].Exp Biol Med(Maywood),2008,233(6):674-688.
[4] SIMOPOULOS A P. An increase in the Omega-6/Omega-3 fatty acid ratio increases the risk for obesity[J].Nutrients, 2016, 8(3): 128.
[5] TERRY P D,ROHAN T E, WOLK A.In takes of fish and marine fatty acids and the rishs of cancers of the breast and prostate and of other hormone-related cancers:a review of the epidemiologic evidence[J].Am J Clin Nutr,2003,77(3):532-543.
[6] COMBA A,MAESTRI D M,BERRA M A,et al.Effect of ω-3 and ω-9 fatty acid rich oils on lipoxygenases and cyclooxygenases enzymes and on the growth of a mammary adenocarcinoma model[J].Lipids Health Dis,2010,9:112.
[7] YAM D,PELED A,SHWIT Z M. Suppression of tumorgrowth and metastasis by dietary fish oil combined with vitamins E and C and cisplatin[J].Cancer Chemother Pharmacol,2001,47(1):34-40.
[8] JEROMSON S,GALLAGHER I J,GALLOWAY S D,et al.Omega-3 fatty acids and skeletal muscle health[J].Mar Drugs,2015,13(11):6977-7004.
[9] BOUSQUET M,GUE K,EMOND V,et al.Transgenic conversion of omega-6 into omega-3 fatty acids in a mouse model of Parkinson’s disease[J].J Lipid Res,2011,52(2):263-271.
[10] HE C,QU X,CUI L,et al.Improved spatial learning performance of fat-1 mice is associated with enhanced neurogenesis and neuritogenesis by docosahexaenoic acid[J]. Proc Natl Acad Sci USA,2009,106(27):11370-11375.
[11] 刘白平,李玉玉,张才,等.Fat-1转基因模型小鼠对脂多糖诱导的抑郁样行为和中枢炎症反应的改善作用[J].广东海洋大学学报,2017,37(3):86-92.
[12] ZOU Z,BELLENGER S,MASSEY K A,et al.Inhibition of the HER2 pathway by n-3 polyunsaturated fatty acids prevents breast cancer in fat-1 ransgenic mice[J].J Lipid Res,2013,54(12):3453-3463.
[13] SUN H,HU Y,GU Z,et al. Endogenous synthesis of n-3 polyunsaturated fatty acids in fat-1 mice is associated with increased mammary gland and liver syndecan-1[J].PLoS One,2011,6(5):e20502.
[14] GU Z N,SUBURU J,CHEN H Q,et al.Mechanisms of Omega-3 polyunsaturated fatty acids in prostate cancer prevention[J].Biomed Res Int,2013,2013: 824563.
[15] 柳欣,欣赵艳,陈平,等.内源性n-3多不饱和脂肪酸对AKT/Ras小鼠肝癌细胞抑制作用[J].肠外与肠内营养,2016,23(4):237-241.
[16] MATTHEW S.Deciphering the role of omega-3 polyunsaturated fatty acid derived lipid mediators in health and disease [J].Proc Nutr Soc,2013,72(4):441-450.
[17] LOPEZ-VICARIO C,ALCARAZ-QUILES J,GARCIA-ALONSO V,et al.Inhibition of soluble epoxide hydrolase modulates inflammation and autophagy in obese adipose tissue and liver: Role for omega-3 epoxides[J].Proc Natl Acad Sci USA,2015,112(2):536-541.
[18] TEAGUE H, FHANER C J,HARRIS M,et al.n-3 PUFAs enhance the frequency of murine B-cell subsets and restore the impairment of antibody production to a T-independent antigen in obesity Heather[J].J Lipid Res,2013,54(11):3130-3138.
[19] DIANA L,BONILLAL,YANG Y F,et al.Transgenic mice enriched in omega-3 fatty acids are more susceptible to pulmonary tuberculosis: impaired resistance to tuberculosis in fat-1 mice[J].J Infect Dis,2010,201(3):399-408.
[20] WANG Y,DANIELA B,XU J H,et al.Regulation of hepatic fatty acid elongase and desaturase expression in diabetes and obesity[J].J Lipid Res,2006,47(9): 2028-2041.
[21] KIRSI H,PIETILA T R,TUULIKKII S,et al.Association of lipidome remodeling in the adipocyte membrane with acquired obesity in humans[J].PLoS Biol,2011, 9(6): e1000623.
[22] 盖晓英,马爽爽,曹锦鹏,等.Fat-1转基因对小鼠饮食诱导肥胖的影响[J].青岛大学医学院学报,2015,53(4):401-404.
[23] LIU X X,PANG D X,YUAN T,et al.N-3 polyunsaturated fatty acids attenuates triglyceride and inflammatory factors level in hfat-1 transgenic pigs[J].Lipids Health Dis,2016,15:89.
[24] 周飞,张晓宏,邹祖全,等.内源性n-3多不饱和脂肪酸对fat-1转基因小鼠血糖血脂的影响[J].宁波大学学报(理工版),2015,28(3):98-102.
[25] CHILTON F H,MURPHY R C,WILSON B A,et al. Diet-gene interactions and PUFA metabolism: a potential contributor to health disparities and human diseases[J]. Nutrients,2014, 6(5):1993-2022.
[26] FENTON J I,HORD N G,GHOSH S,et al. Immunomodulation by dietary long chain omega-3 fatty acid immunomodulation and the potential for adverse health outcomes[J].Prostaglandins Leukot Essent Fatty Acids,2013,89(6):379-390.