灯盏花素注射液对病毒性心肌炎临床疗效具有促进作用
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摘要
为探讨磷酸肌酸与灯盏花素注射液联合治疗病毒性心肌炎(viral myocarditis, VMC)的临床治疗效果及其潜在分子机制,本研究将108例VMC患者随机分为两组,对照组和治疗组,每组均54例。对照组在给予常规对症治疗的基础上加磷酸肌酸;治疗组在对照组的基础上给予灯盏花素注射液,4周为一个疗程。采用试剂盒检测治疗前后患者血清中肌酸激酶(creatine kinase, CK)、磷酸肌酸激酶(creatine phosphate kinase,CPK)、磷酸肌酸激酶同工酶(creatine kinase-myocardial band, CK-MB)和天冬氨酸转氨酶(aspartate transaminase,AST)的水平,检测炎症因子肿瘤坏死因子α(tumor necrosis factor, TNF-α)、白细胞介素6 (interleukin-6,IL-6)和白细胞介素8 (interleukin-8, IL-8)以及超氧化物歧化酶(superoxide dismutase, SOD)的水平。结果显示,治疗4周后,治疗组的总有效率显著高于对照组(p<0.05);两组患者血清中CK、CPK、CK-MB及AST水平均显著下降(p<0.05),TNF-α、IL-6和IL-8的水平均显著下降(p<0.05),SOD水平显著上升(p<0.05),其中治疗组的变化幅度更为明显,说明磷酸肌酸联合灯盏花素注射液治疗VMC具有较好的疗效,可能与降低炎症因子TNF-α、IL-6和IL-8,增加SOD活性有关。
In order to study the clinical curative effect and mechanism of creatine phosphate combined with erigeron injection on the viral myocarditis(VMC), 108 cases of VMC were selected and randomly divided into control group(54 cases) and treatment group(54 cases). The control group was treated with creatine phosphate on the basis of routine symptomatic treatment, and the treatment group was given erigeron injection on the basis of the control group. Four weeks as a course of treatment. The serum creatine kinase(CK), creatine phosphate kinase(CPK),creatine kinase isoenzyme(CK-MB), aspartate aminotransferase(AST), tumor necrosis factor alpha(TNF-α),interleukin 6(IL-6), interleukin 8(IL-8) and superoxide disproportionation enzyme(SOD) of the two groups were compared before and after treatment. The results showed that after 4 weeks of treatment, the total effective rate of the treatment group was significantly higher than that of the control group(p<0.05). Both two groups had lower enzyme activity of CK, CPK, CK-MB and AST and the content of TNF-α, IL-6 and IL-8, and had higher enzyme activity of SOD post treatment, in which these of treatment group change greater. It shows that the combination of creatine phosphate and erigeron injection might have a good clinical effect on VMC cases, which might be associated with reducing TNF-α, IL-6 and IL-8 level, and increasing the activity of SOD.
In order to study the clinical curative effect and mechanism of creatine phosphate combined with erigeron injection on the viral myocarditis(VMC), 108 cases of VMC were selected and randomly divided into control group(54 cases) and treatment group(54 cases). The control group was treated with creatine phosphate on the basis of routine symptomatic treatment, and the treatment group was given erigeron injection on the basis of the control group. Four weeks as a course of treatment. The serum creatine kinase(CK), creatine phosphate kinase(CPK),creatine kinase isoenzyme(CK-MB), aspartate aminotransferase(AST), tumor necrosis factor alpha(TNF-α),interleukin 6(IL-6), interleukin 8(IL-8) and superoxide disproportionation enzyme(SOD) of the two groups were compared before and after treatment. The results showed that after 4 weeks of treatment, the total effective rate of the treatment group was significantly higher than that of the control group(p<0.05). Both two groups had lower enzyme activity of CK, CPK, CK-MB and AST and the content of TNF-α, IL-6 and IL-8, and had higher enzyme activity of SOD post treatment, in which these of treatment group change greater. It shows that the combination of creatine phosphate and erigeron injection might have a good clinical effect on VMC cases, which might be associated with reducing TNF-α, IL-6 and IL-8 level, and increasing the activity of SOD.
引文
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Ding J.D.,Wu X.,and Gu Y.,2001,Detection and significance of antibody against human myocardial mitochondria in virus myocarditis and dilated cardiomyopathy,Zhongguo Xunhuan Zazhi(Chinese Circulation Journal),16(1):38-40(丁建东,吴翔,顾勇,2001,抗人心肌线粒体抗体在病毒性心肌炎和扩张型心肌病中的检测及其意义,中国循环杂志,16(1):38-40)
Ju A.,Li Y.Y.,Qu Z.,and Li Q.,2017,Impact of the herbal breviscapine on the pharmacokinetics of simvastatin in rats:the involvement of CYP3A4,Drug Res.,67(5):271-274
Lin Y.Z.,Lu Z.Y.,Liang X.H.,Li K.,Peng B.,and Gong J.,2016,Effect of breviscapine against hepatic ischemia reperfusion injury,J.Surg.Res.,203(2):268-274
Sukumaran V.,Watanabe K.,Veeraveedu P.T.,Gurusamy N.,Ma M.,Thandavarayan R.A.,Lakshmanan A.P.,Yamaguchi K.,Suzuki K.,and Kodama M.,2011,Olmesartan,an AT1antagonist,attenuates oxidative stress,endoplasmic reticulum stress and cardiac inflammatory mediators in rats with heart failure induced by experimental autoimmune myocarditis,Int.J.Biol.Sci.,7(2):154-167
Sun J.H.,and Zhai S.B.,Progress in the research on viral myocarditispathogenesis,Linchuang Erke Zazhi(The Journal of Clinical Pediatrics),(7):607-612(孙景辉,翟淑波,2012,病毒性心肌炎发病机制的研究进展,临床儿科杂志,(7):607-612)
Tada Y.,and Suzuki J.,2016,Oxidative stress and myocarditis,Curr.Pharm.Des.,22(4):450-471
Wang D.,Li T.,Cui H.,and Zhang Y.,2016b,Analysis of the indicating value of cardiac troponinⅠ,tumor necrosis factor-α,interleukin-18,Mir-1 and Mir-146b for viral myocarditis among children,Cell Physiol.Biochem.,40(6):1325-1333
Wang W.,Ma X.,Han J.,Zhou M.,Ren H.,Pan Q.,Zheng C.,and Zheng Q.,2016a,Neuroprotective effect of scutellarin on ischemic cerebral injury by down-regulating the expression of angiotensin-converting enzyme and AT1 receptor,PLoS One,11(1):e0146197
Xiong Z.,Liu C.,Wang F.,Li C.,Wang W.,Wang J.,and Chen J.,2006,Protective effects of breviscapine on ischemic vascular dementia in rats,Biol.Pharm.Bull.,29(9):1880-1885
Ye Y.,Gong G.,Ochiai K.,Liu J.,and Zhang J.,2001,Highenergy phosphate metabolism and creatine kinase in failing hearts:a new porcine model,Circulation,103(11):1570-1576
Yuan Q.,and Zhang P.,2016,Analysis on effectiveness of Xinjikang Granules combined with creatine phosphate sodium for treating,Chongqing Yixue(Chongqing Medicine),45(10):1343-1344,1347(袁泉,张萍,2016,磷酸肌酸钠联合心肌康颗粒治疗小儿病毒性心肌炎实效性分析,重庆医学,45(10):1343-1344,1347)
Zeng J.,and Cai S.,2017,Breviscapine suppresses the growth of non-small cell lung cancer by enhancing microRNA-7 expression,J.Biosci.,42(1):121-129
Zhou X.H.,Gong M.Y.,and Yan F.X.,2009,Inhibition of breviscapine on apoptosis of cultured myocardial cell of neonatal rat,Zhongchengyao(Chinese Traditional Patent Medicine),31(12):114-125(周晓慧,龚明玉,闫凤霞,2009,灯盏花素对缺氧/复氧诱导培养乳鼠心肌细胞凋亡的抑制作用,中成药,31(12):114-125)
Ding J.D.,Wu X.,and Gu Y.,2001,Detection and significance of antibody against human myocardial mitochondria in virus myocarditis and dilated cardiomyopathy,Zhongguo Xunhuan Zazhi(Chinese Circulation Journal),16(1):38-40(丁建东,吴翔,顾勇,2001,抗人心肌线粒体抗体在病毒性心肌炎和扩张型心肌病中的检测及其意义,中国循环杂志,16(1):38-40)
Ju A.,Li Y.Y.,Qu Z.,and Li Q.,2017,Impact of the herbal breviscapine on the pharmacokinetics of simvastatin in rats:the involvement of CYP3A4,Drug Res.,67(5):271-274
Lin Y.Z.,Lu Z.Y.,Liang X.H.,Li K.,Peng B.,and Gong J.,2016,Effect of breviscapine against hepatic ischemia reperfusion injury,J.Surg.Res.,203(2):268-274
Sukumaran V.,Watanabe K.,Veeraveedu P.T.,Gurusamy N.,Ma M.,Thandavarayan R.A.,Lakshmanan A.P.,Yamaguchi K.,Suzuki K.,and Kodama M.,2011,Olmesartan,an AT1antagonist,attenuates oxidative stress,endoplasmic reticulum stress and cardiac inflammatory mediators in rats with heart failure induced by experimental autoimmune myocarditis,Int.J.Biol.Sci.,7(2):154-167
Sun J.H.,and Zhai S.B.,Progress in the research on viral myocarditispathogenesis,Linchuang Erke Zazhi(The Journal of Clinical Pediatrics),(7):607-612(孙景辉,翟淑波,2012,病毒性心肌炎发病机制的研究进展,临床儿科杂志,(7):607-612)
Tada Y.,and Suzuki J.,2016,Oxidative stress and myocarditis,Curr.Pharm.Des.,22(4):450-471
Wang D.,Li T.,Cui H.,and Zhang Y.,2016b,Analysis of the indicating value of cardiac troponinⅠ,tumor necrosis factor-α,interleukin-18,Mir-1 and Mir-146b for viral myocarditis among children,Cell Physiol.Biochem.,40(6):1325-1333
Wang W.,Ma X.,Han J.,Zhou M.,Ren H.,Pan Q.,Zheng C.,and Zheng Q.,2016a,Neuroprotective effect of scutellarin on ischemic cerebral injury by down-regulating the expression of angiotensin-converting enzyme and AT1 receptor,PLoS One,11(1):e0146197
Xiong Z.,Liu C.,Wang F.,Li C.,Wang W.,Wang J.,and Chen J.,2006,Protective effects of breviscapine on ischemic vascular dementia in rats,Biol.Pharm.Bull.,29(9):1880-1885
Ye Y.,Gong G.,Ochiai K.,Liu J.,and Zhang J.,2001,Highenergy phosphate metabolism and creatine kinase in failing hearts:a new porcine model,Circulation,103(11):1570-1576
Yuan Q.,and Zhang P.,2016,Analysis on effectiveness of Xinjikang Granules combined with creatine phosphate sodium for treating,Chongqing Yixue(Chongqing Medicine),45(10):1343-1344,1347(袁泉,张萍,2016,磷酸肌酸钠联合心肌康颗粒治疗小儿病毒性心肌炎实效性分析,重庆医学,45(10):1343-1344,1347)
Zeng J.,and Cai S.,2017,Breviscapine suppresses the growth of non-small cell lung cancer by enhancing microRNA-7 expression,J.Biosci.,42(1):121-129
Zhou X.H.,Gong M.Y.,and Yan F.X.,2009,Inhibition of breviscapine on apoptosis of cultured myocardial cell of neonatal rat,Zhongchengyao(Chinese Traditional Patent Medicine),31(12):114-125(周晓慧,龚明玉,闫凤霞,2009,灯盏花素对缺氧/复氧诱导培养乳鼠心肌细胞凋亡的抑制作用,中成药,31(12):114-125)