闽江口鳝鱼滩芦苇湿地沉积物甲烷产生与氧化潜力对外源物质输入的响应
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摘要
研究河口湿地沉积物甲烷(CH4)产生和氧化对外源物质输入的响应,对环境保护及温室气体减排具有重要意义.本研究基于室内培养-气相色谱法,探讨了闽江河口半咸水芦苇(Phragmites australis)沼泽湿地沉积物CH4产生与氧化对不同外源物质(底物、电子受体和营养物质)输入的响应.结果表明:CH3OH(500 mg·kg-1)、C3H9N(500 mg·kg-1)和Fe2+(0~500 mg·kg-1)对CH4产生潜力起促进作用(p<0.05);NO-3(0~500 mg·kg-1)、NO-2(0~500 mg·kg-1)、Fe3+(50 mg·kg-1)和NH+4(50~500 mg·kg-1)表现为抑制CH4产生潜力(p<0.05);而0~50 mg·kg-1的CH3OH和C3H9N、0~500 mg·kg-1的CH3COOH、SO2-4、Mn4+、PO3-4和低剂量的NH+4(0~5 mg·kg-1)对CH4产生的影响不显著(p>0.05).实验剂量内(0~500 mg·kg-1),Fe3+和Mn4+的添加可促进CH4氧化(p<0.05);CH3COOH、CH3OH、C3H9N、NO-3、NO-2、SO2-4、NH+4和低剂量的PO3-4(0~50 mg·kg-1)对沉积物CH4氧化潜力均有显著的抑制作用(p<0.05);而Fe2+对CH4氧化没有显著影响(p>0.05).综合分析表明,CH3COOH、CH3OH、C3H9N、NO-3、NO-2、SO2-4、PO3-4、NH+4和Fe2+的输入对沉积物CH4产生和氧化的综合作用为增加CH4排放通量,而Fe3+和Mn4+输入的综合作用则与之相反.
Methane,a powerful greenhouse gas,is both produced and consumed in estuary sediments. Studying the response of methane production and oxidation potential to exogenous substances in estuarine marsh sediments is important to environment protection and greenhouse gas emission reductions.The rates of methane production and oxidation of sediments under the Phragmites australis marsh in the Shanyutan wetland in the Min River estuary were determined using incubation experiment. Different exogenous substances,including substrates,electron acceptors and nutrients,were added to explore theaffecting factors of methane production and oxidation potential. High concentration( 500 mg·kg-1) of methyl alcohol and trimethylamine and 0 ~ 500mg·kg-1of ferrous ion promoted methane production potential,nitrate( 0 ~ 500 mg·kg-1),nitrite( 0~ 500 mg·kg-1),ferric iron( 50 mg·kg-1) and ammonium( 50 ~ 500 mg·kg-1) inhibited methane production potential,while there was no significant influence for methyl alcohol( 0 ~ 50 mg·kg-1),trimethylamine( 0 ~ 50 mg·kg-1),low concentration of ammonium( 0~5 mg·kg-1) and acetic acid,sulfate,manganese ion and phosphate radical at the concentration of 0 ~ 500 mg·kg-1. Furthermore,0 ~ 500 mg·kg-1of ferric iron and manganese ion promoted methane oxidation potential,while methyl alcohol,trimethylamine,nitrate,nitrite,sulfate,ammonium and low concentration of phosphate radical( 0 ~ 50 mg·kg-1) inhibited methane oxidation potential. However,various concentrations of ferrous ion didn't make significant difference on methane oxidation potential. Methane emissions will increase as a response of methane production and oxidation potential to acetic acid,methyl alcohol,trimethylamine,phosphate radical,ammonium and ferrous ion,but will mitigate in response to ferric iron and manganese ion.
Methane,a powerful greenhouse gas,is both produced and consumed in estuary sediments. Studying the response of methane production and oxidation potential to exogenous substances in estuarine marsh sediments is important to environment protection and greenhouse gas emission reductions.The rates of methane production and oxidation of sediments under the Phragmites australis marsh in the Shanyutan wetland in the Min River estuary were determined using incubation experiment. Different exogenous substances,including substrates,electron acceptors and nutrients,were added to explore theaffecting factors of methane production and oxidation potential. High concentration( 500 mg·kg-1) of methyl alcohol and trimethylamine and 0 ~ 500mg·kg-1of ferrous ion promoted methane production potential,nitrate( 0 ~ 500 mg·kg-1),nitrite( 0~ 500 mg·kg-1),ferric iron( 50 mg·kg-1) and ammonium( 50 ~ 500 mg·kg-1) inhibited methane production potential,while there was no significant influence for methyl alcohol( 0 ~ 50 mg·kg-1),trimethylamine( 0 ~ 50 mg·kg-1),low concentration of ammonium( 0~5 mg·kg-1) and acetic acid,sulfate,manganese ion and phosphate radical at the concentration of 0 ~ 500 mg·kg-1. Furthermore,0 ~ 500 mg·kg-1of ferric iron and manganese ion promoted methane oxidation potential,while methyl alcohol,trimethylamine,nitrate,nitrite,sulfate,ammonium and low concentration of phosphate radical( 0 ~ 50 mg·kg-1) inhibited methane oxidation potential. However,various concentrations of ferrous ion didn't make significant difference on methane oxidation potential. Methane emissions will increase as a response of methane production and oxidation potential to acetic acid,methyl alcohol,trimethylamine,phosphate radical,ammonium and ferrous ion,but will mitigate in response to ferric iron and manganese ion.
引文
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Chidthaisong A,Conrad R.2000.Turnover of glucose and acetate coupledto reduction of nitrate,ferric iron and sulfate and to methanogenesisin anoxic rice field soil[J].FEMS Microbiology Ecology,31(1):73-86
丁维新,蔡祖聪.2001.氮肥对土壤氧化大气甲烷影响的机制[J].农村生态环境,17(3):30-34
Eriksson T,quist M G,Nilsson M B.2010.Production and oxidation ofmethane in a boreal mire after a decade of increased temperature andnitrogen and sulfur deposition[J].Global Change Biology,16(7):2130-2144
高君颖.2012.闽江河口芦苇湿地土壤甲烷产生与氧化潜力研究[D].福州:福建师范大学.13-14
葛瑞娟,宋长春,杨桂生,等.2010.氮输入对三江湿地小叶章不同生长阶段土壤产生CH4能力的影响[J].环境科学学报,30(10):2070-2077
国家海洋局.2013.2013年中国海洋环境状况公报[R/OL].http://www.soa.gov.cn/zwgk/hygb/zghyhjzlgb/201403/t20140324_31065.html
Hanson R S,Hanson T E.1996.Methanotrophic bacteria[J].Microbiological Reviews,60(2):439-471
Im J,Lee S W,Yoon S,et al.2011.Characterization of a novel facultativeMethylocystis species capable of growth on methane,acetate andethanol[J].Environmental Microbiology Reports,3(2):174-181
IPCC.2013.Climate Change 2013:The Physical Science Basis.Contribution of Working Group I to the Fifth Assessment Report ofthe Intergovernmental Panel on Climate Change[R].TS17.Cambridge,United Kingdom+New York,NY,USA:CambridgeUniversity Press
姜欢欢,孙志高,王玲玲,等.2012.黄河口潮滩湿地土壤甲烷产生潜力及其对有机物和氮输入响应的初步研究[J].湿地科学,10(4):451-458
Kaku N,Ueki A,Ueki K,et al.2005.Methanogenesis as an importantterminal electron accepting process in estuarine sediment at themouth of Orikasa River[J].Microbes and Environments,20(1):41-52
Le Mer J,Roger P.2001.Production,oxidation,emission and consumptionof methane by soils:A review[J].European Journal of Soil Biology,37(1):25-50
Lipson D,Miller K,Lai C.2013.Methane Suppression:The Impacts of Fe(III)and Humic Acids on Net Methane Flux from Arctic TundraWetlands in Alaska and Finland[R].B32C-02.San Diego,CA,USA:American Geophysical Union
柳铮铮.2009.酸沉降对闽江河口湿地CH4产生排放和碳输出的影响[D].福州:福建师范大学.31-36
Lovley D R,Coates J D,Blunt-Harris E L,et al.1996.Humic substancesas electron acceptors for microbial respiration[J].Nature,382(6590):445-448
Lyimo T J,Pol A,Op Den Camp H J M.2002.Sulfate reduction andmethanogenesis in sediments of Mtoni mangrove forest,Tanzania[J].AMBIO:A Journal of the Human Environment,31(7):614-616
牟晓杰,刘兴土,仝川,等.2012.闽江河口短叶茳芏湿地CH4和N2O排放对氮输入的短期响应[J].环境科学,33(7):2482-2489
Odum E P,Barrett G W.2009.生态学基础(第5版)[M].陆健健,王伟,王天慧,等译.北京:高等教育出版社.377
Raghoebarsing A A,Pol A,Van De Pas-Schoonen K T,et al.2006.Amicrobial consortium couples anaerobic methane oxidation todenitrification[J].Nature,440(7086):918-921
Sakai S,Ehara M,Tseng I C,et al.2012.Methanolinea mesophila sp.nov.,a hydrogenotrophic methanogen isolated from rice field soil,andproposal of the archaeal family Methanoregulaceae fam.nov.within theorder Methanomicrobiales[J].International Journal of Systematicand Evolutionary Microbiology,62(Pt 6):1389-1395
Segarra K E,Comerford C,Slaughter J,et al.2013.Impact of electronacceptor availability on the anaerobic oxidation of methane in coastalfreshwater and brackish wetland sediments[J].Geochimica etCosmochimica Acta,115:15-30
孙万龙,孙志高,孙文广,等.2014.黄河口潮滩湿地土壤CH4氧化潜力及其对有机物输入的响应[J].草业学报,23(1):104-112
孙志高,牟晓杰,王玲玲.2009.滨海湿地生态系统N2O排放研究进展[J].海洋环境科学,29(1):159-164
佘晨兴,仝川.2011.自然湿地土壤产甲烷菌和甲烷氧化菌多样性的分子检测[J].生态学报,31(14):4126-4135
佘晨兴,仝川.2012.闽江口芦苇沼泽湿地土壤产甲烷菌群落结构的垂直分布[J].生态学报,32(17):5299-5308Sutula M,Day J,Cable J,et al.2001.Hydrological and nutrient budgets of freshwaterand estuarine wetlands of Taylor Slough in Southern Everglades,Florida(U.S.A)[J].Biogeochemistry,56(3):287-310
Syvsalo E,Regina K,Turtola E,et al.2006.Fluxes of nitrous oxide andmethane,and nitrogen leaching from organically and conventionallycultivated sandy soil in western Finland[J].Agriculture,Ecosystems&Environment,113(1/4):342-348
仝川,陈重安,黄佳芳.2011.河口沼泽土壤醋酸发酵途径产生的甲烷及基质添加对甲烷产生的刺激作用[J].湿地科学,9(4):305-313
仝川,黄佳芳,王维奇,等.2012.闽江口半咸水芦苇潮汐沼泽湿地甲烷动态[J].地理学报,67(9):1165-1180
王维奇,李鹏飞,曾从盛,等.2013.铁炉渣施加对稻田甲烷产生、氧化与排放的影响[J].生态学报,33(5):1578-1583
Wassmann R,Neue H U,Bueno C,et al.1998.Methane productioncapacities of different rice soils derived from inherent and exogenoussubstrates[J].Plant and Soil,203(2):227-237
Weslien P,Kasimir Klemedtsson,Brjesson G,et al.2009.Strong p Hinfluence on N2O and CH4fluxes from forested organic soils[J].European Journal of Soil Science,60(3):311-320
Yang N,LüF,He P J,et al.2011.Response of methanotrophs andmethane oxidation on ammonium application in landfill soils[J].Applied Microbiology and Biotechnology,92(5):1073-1082
贠娟莉,王艳芬,张洪勋.2013.好氧甲烷氧化菌生态学研究进展[J].生态学报,33(21):6774-6785曾从盛,王维奇,仝川.2008.不同电子受体及盐分输入对河口湿地土壤甲烷产生潜力的影响[J].地理研究,27(6):1321-1330
曾从盛,张林海,王天鹅,等.2012.闽江河口湿地植物枯落物立枯和倒伏分解主要元素动态[J].生态学报,32(20):6289-6299
曾从盛,王维奇,仝川.2008.不同电子受体及盐分输入对河口湿地土壤甲烷产生潜力的影响[J].地理研究,27(6):1321-1330
Beal E J,House C H,Orphan V J.2009.Manganese-and iron-dependentmarine methane oxidation[J].Science,325(5937):184-187
Bridgham S D,Megonigal J P,Keller J K,et al.2006.The carbon balanceof North American wetlands[J].Wetlands,26(4):889-916
Bridgham S D,Cadillo-Quiroz H,Keller J K,et al.2013.Methaneemissions from wetlands:biogeochemical,microbial,and modelingperspectives from local to global scales[J].Global Change Biology,19(5):1325-1346
Chidthaisong A,Conrad R.2000.Turnover of glucose and acetate coupledto reduction of nitrate,ferric iron and sulfate and to methanogenesisin anoxic rice field soil[J].FEMS Microbiology Ecology,31(1):73-86
丁维新,蔡祖聪.2001.氮肥对土壤氧化大气甲烷影响的机制[J].农村生态环境,17(3):30-34
Eriksson T,quist M G,Nilsson M B.2010.Production and oxidation ofmethane in a boreal mire after a decade of increased temperature andnitrogen and sulfur deposition[J].Global Change Biology,16(7):2130-2144
高君颖.2012.闽江河口芦苇湿地土壤甲烷产生与氧化潜力研究[D].福州:福建师范大学.13-14
葛瑞娟,宋长春,杨桂生,等.2010.氮输入对三江湿地小叶章不同生长阶段土壤产生CH4能力的影响[J].环境科学学报,30(10):2070-2077
国家海洋局.2013.2013年中国海洋环境状况公报[R/OL].http://www.soa.gov.cn/zwgk/hygb/zghyhjzlgb/201403/t20140324_31065.html
Hanson R S,Hanson T E.1996.Methanotrophic bacteria[J].Microbiological Reviews,60(2):439-471
Im J,Lee S W,Yoon S,et al.2011.Characterization of a novel facultativeMethylocystis species capable of growth on methane,acetate andethanol[J].Environmental Microbiology Reports,3(2):174-181
IPCC.2013.Climate Change 2013:The Physical Science Basis.Contribution of Working Group I to the Fifth Assessment Report ofthe Intergovernmental Panel on Climate Change[R].TS17.Cambridge,United Kingdom+New York,NY,USA:CambridgeUniversity Press
姜欢欢,孙志高,王玲玲,等.2012.黄河口潮滩湿地土壤甲烷产生潜力及其对有机物和氮输入响应的初步研究[J].湿地科学,10(4):451-458
Kaku N,Ueki A,Ueki K,et al.2005.Methanogenesis as an importantterminal electron accepting process in estuarine sediment at themouth of Orikasa River[J].Microbes and Environments,20(1):41-52
Le Mer J,Roger P.2001.Production,oxidation,emission and consumptionof methane by soils:A review[J].European Journal of Soil Biology,37(1):25-50
Lipson D,Miller K,Lai C.2013.Methane Suppression:The Impacts of Fe(III)and Humic Acids on Net Methane Flux from Arctic TundraWetlands in Alaska and Finland[R].B32C-02.San Diego,CA,USA:American Geophysical Union
柳铮铮.2009.酸沉降对闽江河口湿地CH4产生排放和碳输出的影响[D].福州:福建师范大学.31-36
Lovley D R,Coates J D,Blunt-Harris E L,et al.1996.Humic substancesas electron acceptors for microbial respiration[J].Nature,382(6590):445-448
Lyimo T J,Pol A,Op Den Camp H J M.2002.Sulfate reduction andmethanogenesis in sediments of Mtoni mangrove forest,Tanzania[J].AMBIO:A Journal of the Human Environment,31(7):614-616
牟晓杰,刘兴土,仝川,等.2012.闽江河口短叶茳芏湿地CH4和N2O排放对氮输入的短期响应[J].环境科学,33(7):2482-2489
Odum E P,Barrett G W.2009.生态学基础(第5版)[M].陆健健,王伟,王天慧,等译.北京:高等教育出版社.377
Raghoebarsing A A,Pol A,Van De Pas-Schoonen K T,et al.2006.Amicrobial consortium couples anaerobic methane oxidation todenitrification[J].Nature,440(7086):918-921
Sakai S,Ehara M,Tseng I C,et al.2012.Methanolinea mesophila sp.nov.,a hydrogenotrophic methanogen isolated from rice field soil,andproposal of the archaeal family Methanoregulaceae fam.nov.within theorder Methanomicrobiales[J].International Journal of Systematicand Evolutionary Microbiology,62(Pt 6):1389-1395
Segarra K E,Comerford C,Slaughter J,et al.2013.Impact of electronacceptor availability on the anaerobic oxidation of methane in coastalfreshwater and brackish wetland sediments[J].Geochimica etCosmochimica Acta,115:15-30
孙万龙,孙志高,孙文广,等.2014.黄河口潮滩湿地土壤CH4氧化潜力及其对有机物输入的响应[J].草业学报,23(1):104-112
孙志高,牟晓杰,王玲玲.2009.滨海湿地生态系统N2O排放研究进展[J].海洋环境科学,29(1):159-164
佘晨兴,仝川.2011.自然湿地土壤产甲烷菌和甲烷氧化菌多样性的分子检测[J].生态学报,31(14):4126-4135
佘晨兴,仝川.2012.闽江口芦苇沼泽湿地土壤产甲烷菌群落结构的垂直分布[J].生态学报,32(17):5299-5308Sutula M,Day J,Cable J,et al.2001.Hydrological and nutrient budgets of freshwaterand estuarine wetlands of Taylor Slough in Southern Everglades,Florida(U.S.A)[J].Biogeochemistry,56(3):287-310
Syvsalo E,Regina K,Turtola E,et al.2006.Fluxes of nitrous oxide andmethane,and nitrogen leaching from organically and conventionallycultivated sandy soil in western Finland[J].Agriculture,Ecosystems&Environment,113(1/4):342-348
仝川,陈重安,黄佳芳.2011.河口沼泽土壤醋酸发酵途径产生的甲烷及基质添加对甲烷产生的刺激作用[J].湿地科学,9(4):305-313
仝川,黄佳芳,王维奇,等.2012.闽江口半咸水芦苇潮汐沼泽湿地甲烷动态[J].地理学报,67(9):1165-1180
王维奇,李鹏飞,曾从盛,等.2013.铁炉渣施加对稻田甲烷产生、氧化与排放的影响[J].生态学报,33(5):1578-1583
Wassmann R,Neue H U,Bueno C,et al.1998.Methane productioncapacities of different rice soils derived from inherent and exogenoussubstrates[J].Plant and Soil,203(2):227-237
Weslien P,Kasimir Klemedtsson,Brjesson G,et al.2009.Strong p Hinfluence on N2O and CH4fluxes from forested organic soils[J].European Journal of Soil Science,60(3):311-320
Yang N,LüF,He P J,et al.2011.Response of methanotrophs andmethane oxidation on ammonium application in landfill soils[J].Applied Microbiology and Biotechnology,92(5):1073-1082
贠娟莉,王艳芬,张洪勋.2013.好氧甲烷氧化菌生态学研究进展[J].生态学报,33(21):6774-6785曾从盛,王维奇,仝川.2008.不同电子受体及盐分输入对河口湿地土壤甲烷产生潜力的影响[J].地理研究,27(6):1321-1330
曾从盛,张林海,王天鹅,等.2012.闽江河口湿地植物枯落物立枯和倒伏分解主要元素动态[J].生态学报,32(20):6289-6299
曾从盛,王维奇,仝川.2008.不同电子受体及盐分输入对河口湿地土壤甲烷产生潜力的影响[J].地理研究,27(6):1321-1330