千金子制霜前后提取物对4类肠道菌影响的体外研究
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摘要
目的研究并对比千金子制霜前后提取物对正常SD大鼠肠道菌群的影响。方法采用酶标比浊法,体外不同浓度千金子制霜前后提取物作用下的细菌生长曲线来研究制霜对长双歧杆菌、嗜酸乳杆菌、大肠埃希菌和粪肠球菌4种代表性肠道菌的作用,分析其对4种肠道菌生长的影响。结果千金子生品提取物和霜品提取物对4种肠道菌均有抑制作用,且随着给药浓度的增大,抑制作用增强。相同生药量下,千金子生品提取物对4种肠道菌的抑制作用均大于霜品提取物,且低剂量下会使条件致病菌粪肠球菌和大肠埃希菌的数量减少。推测千金子制霜前后均会一定程度影响肠道菌群的稳态,制霜后对4种肠道菌的影响弱于生品,与千金子制霜后泻下作用缓和的研究结果相吻合。结论从肠道微生态角度为切入点,考察千金子制霜前后提取物对肠道菌群数量和生长情况的干预作用,可初步揭示千金子制霜与肠道菌群数量和结构变化可能存在的相关性,为深入揭示千金子制霜减毒微生物学机制奠定基础。
Objective To study and compare the effects of Semen Euphorbiae and Semen Euphorbiae Pulveratun on intestinal flora in vitro.Methods A microtiter plate assay was employed to systematically assess the effects of Semen Euphorbiae and Semen Euphorbiae Pulveratun on the intestinal flora of SD rats in vitro.The growth curves of Escherichia coli,Bifidobacterium longum,Lactobacillus acidophilus and Enterococcus faecalis before and after treated with Semen Euphorbiae and Semen Euphorbiae Pulveratun were obtained.Results The four species of intestinal bacteria were inhibited by Semen Euphorbiae and Semen Euphorbiae Pulveratun,and the inhibitory effect enhanced with the increase of the concentration.The inhibitory effect of Semen Euphorbiae on the four species of bacteria was greater than that of Semen Euphorbiae Pulveratun.The numbers of conditional pathogens,Enterococcus faecalis and E.coli,were reduced by low dose of Semen Euphorbiae extract.It was suggested that Semen Euphorbiae could affect the steady state of intestinal flora before and after powderization,and the effect of Semen Euphorbiae Pulveratun was weaker than that of the Semen Euphorbiae.This result was consist with the toxicity-attenuated mechanism preparing process of Semen Euphorbiae.Conclusion This study revealed the possible correlation between the quantities and structure of intestinal microflora and Semen Euphorbiae,and laid foundation for the study on microbiological mechanism of the attenuation of Semen Euphorbiae after powderization.
Objective To study and compare the effects of Semen Euphorbiae and Semen Euphorbiae Pulveratun on intestinal flora in vitro.Methods A microtiter plate assay was employed to systematically assess the effects of Semen Euphorbiae and Semen Euphorbiae Pulveratun on the intestinal flora of SD rats in vitro.The growth curves of Escherichia coli,Bifidobacterium longum,Lactobacillus acidophilus and Enterococcus faecalis before and after treated with Semen Euphorbiae and Semen Euphorbiae Pulveratun were obtained.Results The four species of intestinal bacteria were inhibited by Semen Euphorbiae and Semen Euphorbiae Pulveratun,and the inhibitory effect enhanced with the increase of the concentration.The inhibitory effect of Semen Euphorbiae on the four species of bacteria was greater than that of Semen Euphorbiae Pulveratun.The numbers of conditional pathogens,Enterococcus faecalis and E.coli,were reduced by low dose of Semen Euphorbiae extract.It was suggested that Semen Euphorbiae could affect the steady state of intestinal flora before and after powderization,and the effect of Semen Euphorbiae Pulveratun was weaker than that of the Semen Euphorbiae.This result was consist with the toxicity-attenuated mechanism preparing process of Semen Euphorbiae.Conclusion This study revealed the possible correlation between the quantities and structure of intestinal microflora and Semen Euphorbiae,and laid foundation for the study on microbiological mechanism of the attenuation of Semen Euphorbiae after powderization.
引文
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[7]LIU Shiwang,XU Yanxia,SHI Hongwu.Effect of ethanol extract of Saxifraga stolonifera Meerb on growth curve of bacterium[J].J Anhui Agric Sci,2007,35(4):943-944,946.(in Chinese)刘世旺,徐艳霞,石宏武.虎耳草乙醇提取物对细菌生长曲线的影响[J].安徽农业科学,2007,35(4):943-944,946.
[8]Patra JK,Das G,Baek KH.Chemical composition and antioxidant and antibacterial activities of an essential oil extracted from an edible seaweed,Laminaria japonica L.[J].Molecules,2015,20(7):12093-12113.
[9]Bekkahadji F,Bombarda I,Touati A.Antibacterial activity against methicillin-resistant of five essential oils from Algerian medicinal plants(Lamiaceae)[J].J Essent Oil Res,2016,28(6):518-527.
[10]ZHOU Changlin.Experiment and Guide for Microbiology[M].2nd ed.Harbin:China Press of Traditional Chinese Medicine,2010:110.(in Chinese)周长林.微生物学实验与指导[M].2版.哈尔滨:中国中医药科技出版社,2010:110.
[11]TAN Wenying,LI Zhong,ZHANG Weiwei,et al.Antibacterial activity of different processed products of Dendranthema indicum[J].Gansu Med J,2016,35(1):1-4.(in Chinese)谭文影,李钟,张维维,等.野菊花不同炮制品提取物抑菌效果比较研究[J].甘肃医药,2016,35(1):1-4.
[2]张照楠,陈瑞雪,王跃飞,等.药物与肠道菌群的相互作用[J].中药材,2015,38(6):1319-1323.
[3]Gormez A,Bozari S,Yanmis D,et al.Chemical composition and antibacterial activity of essential oils of two species of lamiaceae against phytopathogenic bacteria[J].Polish J Microbiol,2015,64(2):121-127.
[4]CHEN Mo,WANG Zhiwei,HU Changying,et al.Rapid evaluating of antimicrobial activity of vanillin with the microplate reader in 96-cell plate[J].Food&Ferment Indust,2009,35(5):63-66.(in Chinese)陈默,王志伟,胡长鹰,等.酶标仪法快速评价香兰素的抑菌活性[J].食品与发酵工业,2009,35(5):63-66.
[5]LI Fengguang.Screening of compound Mongolian medicine treating endometritis of cows and researching of its mechanism on anti-bacteria[D].Hohhot:Inner Mongolia Agricultural University,2015.(in Chinese)李风光.奶牛子宫内膜炎抗菌蒙药复方的筛选及其抗菌作用的研究[D].呼和浩特:内蒙古农业大学,2015.
[6]WENG Peifang,JIANG Huazhen,FENG Fengqin,et al.Evaluating of antimicrobial activity of glycerol monlaurate against Staphylococcus carnosu with the microplate reader[J].J Chin Inst Food Sci&Technol,2012,12(5):188-194.(in Chinese)翁佩芳,江华珍,冯凤琴,等.酶标比浊法评价月桂酸单甘油酯对肉葡萄球菌的抑菌活性[J].中国食品学报,2012,12(5):188-194.
[7]LIU Shiwang,XU Yanxia,SHI Hongwu.Effect of ethanol extract of Saxifraga stolonifera Meerb on growth curve of bacterium[J].J Anhui Agric Sci,2007,35(4):943-944,946.(in Chinese)刘世旺,徐艳霞,石宏武.虎耳草乙醇提取物对细菌生长曲线的影响[J].安徽农业科学,2007,35(4):943-944,946.
[8]Patra JK,Das G,Baek KH.Chemical composition and antioxidant and antibacterial activities of an essential oil extracted from an edible seaweed,Laminaria japonica L.[J].Molecules,2015,20(7):12093-12113.
[9]Bekkahadji F,Bombarda I,Touati A.Antibacterial activity against methicillin-resistant of five essential oils from Algerian medicinal plants(Lamiaceae)[J].J Essent Oil Res,2016,28(6):518-527.
[10]ZHOU Changlin.Experiment and Guide for Microbiology[M].2nd ed.Harbin:China Press of Traditional Chinese Medicine,2010:110.(in Chinese)周长林.微生物学实验与指导[M].2版.哈尔滨:中国中医药科技出版社,2010:110.
[11]TAN Wenying,LI Zhong,ZHANG Weiwei,et al.Antibacterial activity of different processed products of Dendranthema indicum[J].Gansu Med J,2016,35(1):1-4.(in Chinese)谭文影,李钟,张维维,等.野菊花不同炮制品提取物抑菌效果比较研究[J].甘肃医药,2016,35(1):1-4.