HPLC法同时测定麒麟丸中的3种成分
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摘要
目的 :建立HPLC法同时测定麒麟丸(制何首乌、墨旱莲、淫羊藿等)中3种成分的含量。方法 :麒麟丸50%乙醇提取液的分析采用Vensuil XBP C_(18)(4.6 mm×250 mm,5 mm);以含0.05%三氟乙酸的水溶液(A)和含0.05%三氟乙酸的乙腈溶液(B)为流动相,梯度洗脱;体积流量1.2 ml/min;柱温30℃;检测波长220 nm。结果 :橙皮苷、丹酚酸B和淫羊藿苷分别在6～18 mg/ml(r=0.999 8),3～9 mg/ml(r=0.999 8),2～6 mg/ml(r=0.999 9)范围内线性关系良好,平均加样回收率分别为95.9%、93.6%、92.4%,RSD分别为1.6%、3.0%、4.6%。结论 :该方法准确、重复性好,可用于质量控制。
Objective: To establish an HPLC method for the simultaneous determination of the contents of three constituents in Qilin pills(Polygoni Multiflori Radix Praeparata, Ecliptae Herba, Epimedii Folium). Methods: The analysis of 50%ethanol extract of Qilin pills was performed on a Vensuil XBP C_(18)(4.6 mm×250 mm,5 mm)by gradient elution with the mobile phase comprising of acetonitrile-0.05% trifluoroacetic acid at volume flow 1.2 ml/min, 30 ℃ and detection wavelength 220 nm.Results: Hesperidin, salvianolic acid B and icariin showed good linear relationship in the ranges of 6-18 mg/ml(r=0.999 8),3-9 mg/ml(r=0.999 8),2-6 mg/ml(r=0.999 9)with average recoveries of 95.9%, 93.6% and 92.4% and RSDs of 1.6%, 3.0% and 4.6%,respectively. Conclusion: This method is accurate and reproducible and can be used for the quality control of Qilin pills.
Objective: To establish an HPLC method for the simultaneous determination of the contents of three constituents in Qilin pills(Polygoni Multiflori Radix Praeparata, Ecliptae Herba, Epimedii Folium). Methods: The analysis of 50%ethanol extract of Qilin pills was performed on a Vensuil XBP C_(18)(4.6 mm×250 mm,5 mm)by gradient elution with the mobile phase comprising of acetonitrile-0.05% trifluoroacetic acid at volume flow 1.2 ml/min, 30 ℃ and detection wavelength 220 nm.Results: Hesperidin, salvianolic acid B and icariin showed good linear relationship in the ranges of 6-18 mg/ml(r=0.999 8),3-9 mg/ml(r=0.999 8),2-6 mg/ml(r=0.999 9)with average recoveries of 95.9%, 93.6% and 92.4% and RSDs of 1.6%, 3.0% and 4.6%,respectively. Conclusion: This method is accurate and reproducible and can be used for the quality control of Qilin pills.
引文
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[11]赵文静,王历,王芝兰,等.淫羊藿的药理作用及临床应用研究进展[J].中医药信息, 2016, 33(2):105-108.
[2]陈小凯,段启,何伟,等.麒麟丸质量标准提高的研究[J].中国药房, 2013, 24(23):2164-2167.
[3]江秀娟,陈新,梁华伦,等. HPLC法测定麒麟丸中2, 3, 5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量[J].亚太传统医药, 2009, 5(10):28-30.
[4]段启,庄义修,姚丽梅,等.麒麟丸定性定量方法的研究[J].中南药学, 2012, 10(12):919-922.
[5]张冬松,高慧媛,吴立军.橙皮苷的药理活性研究进展[J].中国现代中药, 2006, 8(7):25-27.
[6]钱俊臻,王伯初.橙皮苷的药理作用研究进展[J].天然产物研究与开发, 2010, 22(1):176-180.
[7]湛月娥,徐江平.丹酚酸B药理作用的研究进展[J].华南国防医学杂志, 2007, 21(2):37-54.
[8]赵先,王婧雯,陆杨,等.丹酚酸B药理作用的研究进展[J].西北药学杂志, 2015, 30(1):107-110.
[9]孟宁,孔凯,李师翁.淫羊藿属植物化学成分及药理活性研究进展[J].西北植物学, 2010, 30(5):1063-1073.
[10]李艳,于涛,苗明三.淫羊藿的化学、药理与临床应用分析[J].中医学报, 2017, 32(4):619-622.
[11]赵文静,王历,王芝兰,等.淫羊藿的药理作用及临床应用研究进展[J].中医药信息, 2016, 33(2):105-108.