摘要
目的探讨胃癌组织中脆性组氨酸三联体(FHIT)、Bcl-2蛋白表达与幽门螺杆菌(Hp)感染的关系。方法收集2013年1月~2017年1月于解放军第四六四医院行胃癌根治术的80例胃癌组织标本,另选其中43例癌旁组织。采用快速尿素酶试纸和Warthin-Starry染色,检测组织标本是否感染Hp,采用免疫组化染色方法检测组织标本中FHIT和Bcl-2的表达。结果胃癌组织FHIT阳性表达率低于癌旁组织(P <0.05),胃癌组织Bcl-2阳性表达率高于癌旁组织(P <0.05)。胃癌组织中FHIT的表达与淋巴转移、远处转移、分化程度、浸润程度和Hp感染均有关(均P <0.05),Bcl-2的表达与肿瘤直径、分化程度、浸润程度和Hp感染有关(均P <0.05)。FHIT的阳性率与Hp感染呈负相关(r=-0.295,P <0.05),Bcl-2的阳性率与Hp感染呈正相关(r=0.393,P <0.05)。结论胃癌组织中FHIT蛋白表达减弱,Bcl-2蛋白表达增强,且均与Hp感染关系密切,三者相互作用导致了胃癌的发生。
Objective To investigate the relationship between fragile histidine triad(FHIT) and Bcl-2 protein expression in gastric cancer tissues and helicobacter pylori(Hp) infection. Methods From January 2013 to January 2017, 80 gastric carcinoma tissue samples were collected after radical gastrectomy in the 464 th Hospital of PLA, and 43 paracancerous tissues were selected. Rapid urease assay paper and Warthin-Starry staining were used to detect whether the tissue samples were infected with Hp, and the expressions of FHIT and Bcl-2 in the tissue samples were detected by immunohistochemical staining. Results FHIT positive expression rate in gastric carcinoma tissues was lower than that in para-carcinoma tissues(P < 0.05), Bcl-2 positive expression rate in gastric carcinoma tissues was higher than that in para-carcinoma tissues(P < 0.05). The expression of FHIT in gastric carcinoma tissues was related to lymphatic metastasis, distant metastasis, degree of differentiation, degree of infiltration and Hp infection(all P < 0.05). Bcl-2 expression was related to tumor diameter, differentiation degree, infiltration degree and Hp infection(all P < 0.05). The positive rate of FHIT was negatively correlated with Hp infection(r =-0.295, P < 0.05), and the positive rate of Bcl-2 was positively correlated with Hp infection(r = 0.393, P < 0.05). Conclusion The expression of FHIT protein in gastric carcinoma tissues is weakened, and the expression of Bcl-2 protein is enhanced, which are closely related to Hp infection, and the three interactions lead to the occurrence of gastric carcinoma.
引文
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