电化学方法研究脱脂乳在金电极表面的吸附
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摘要
本文研究脱脂乳在金电极表面的吸附,并探讨脱脂乳的变化对吸附的影响。以微分电容法研究脱脂乳在金电极表面的吸附,并用循环伏安法和交流阻抗表征电极吸附,采用石英晶体微天平研究不同脱脂乳在金电极表面的界面吸附。结果表明:在一定浓度范围内,脱脂乳在金电极表面吸附是单分子层吸附,符合Langmuir吸附等温方程式;随着脱脂乳浓度的增加,脱脂乳在金晶体表面逐步达到饱和吸附;在不同p H缓冲溶液的吸附量也不同,在p H为5时吸附量最大;在同一稀释浓度及p H条件下,吸附量随着掺杂离子强度的增加呈现先增大后减小的规律。
In the study,the adsorption of skimmed milk at Au electrode was nvestigated. Differential capacitance were used to study skimmed milk of different concentrations dispersion adsorption property on the surface of Au electrode,the adsorption behavior of milk on the Au electrode was characterized by cyclic voltammetry and electrochemical impedance spectroscopy.The adsorption behavior of different skimmed milk dispersion on gold crystal surface were researched by QCM. The results showed that within a certain concentration range,skimmed milk dispersion was monomolecular layer adsorption on the surface of the Au electrodes and in line with the Langmuir adsorption isotherm equation. Skimmed milk on the adsorption of gold crystal surface gradually reached saturation with the increasing skimmed milk concentration. In different p H buffer solutions,the adsorption quantity of milk on gold crystal surface was different,with the maximum adsorption in the p H of 5. At the same concentration and p H,adsorption capacity increased firstly and then decreased with the increase of ionic strength.
In the study,the adsorption of skimmed milk at Au electrode was nvestigated. Differential capacitance were used to study skimmed milk of different concentrations dispersion adsorption property on the surface of Au electrode,the adsorption behavior of milk on the Au electrode was characterized by cyclic voltammetry and electrochemical impedance spectroscopy.The adsorption behavior of different skimmed milk dispersion on gold crystal surface were researched by QCM. The results showed that within a certain concentration range,skimmed milk dispersion was monomolecular layer adsorption on the surface of the Au electrodes and in line with the Langmuir adsorption isotherm equation. Skimmed milk on the adsorption of gold crystal surface gradually reached saturation with the increasing skimmed milk concentration. In different p H buffer solutions,the adsorption quantity of milk on gold crystal surface was different,with the maximum adsorption in the p H of 5. At the same concentration and p H,adsorption capacity increased firstly and then decreased with the increase of ionic strength.
引文
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[2]Bueno L,de Araujo W R,Salles M O,et al.Voltammetric Electronic Tongue for Discrimination of Milk Adulterated with Urea,Formaldehyde and Melamine[J].Chemosensors,2014,2(4):251-266.
[3]Bueno L,de Araujo W R,Salles M O,et al.Voltammetric electronic tongue for discrimination of milk adulterated with urea,formaldehyde and melamine[J].Chemosensors,2014,2(4):251-266.
[4]Wei Z,Wang J,Zhang X.Monitoring of quality and storage time of unsealed pasteurized milk by voltammetric electronic tongue[J].Electrochimica Acta,2013,88:231-239.
[5]黄赣辉,邓少平.人工智能味觉系统:概念,结构与方法[J].化学进展,2006,18(4):494-500.
[6]曾祥盛,邓丹雯,黄赣辉,等.传感器阵列对生乳及其若干形态的差异性识别[J].食品科学,2013,34(12):208-212.
[7]黄赣辉,石磊,曾祥盛,等.综合型传感器阵列对掺杂生乳的识别[J].食品工业科技,2014,35(6):63-66.
[8]Farcas M,Cosman N P,Ting D K,et al.A comparative study of electrochemical techniques in investigating the adsorption behaviour of fibrinogen on platinum[J].Journal of Electroanalytical Chemistry,2010,649(1):206-218.
[9]Silva D J R,Diniz F B.Electrochemical Impedance Spectroscopy Study of Concanavalin A Adsorption on Glassy Carbon Electrode:An Analysis of Capacitance Dispersion[J].Electrochimica Acta,2014,119:99-105.
[10]Frumkin A N,Damaskin B B,Bockris J O M,et al.Modern Aspects of Electrochemistry[J].Butterworths,1964:149.
[11]Gettens R T T,Bai Z,Gilbert J L.Quantification of the kinetics and thermodynamics of protein adsorption using atomic force microscopy[J].Journal of Biomedical Materials Research Part A,2005,72(3):246-257.
[12]Bajpai A K,Mishra D D.Adsorption of fibrinogen onto macroporous,biocompatible sponges based on poly(2-hydroxyethyl methacrylate)[J].Journal of Applied Polymer Science,2006,102(2):1341-1355.
[13]Farcas M,Cosman N P,Ting D K,et al.A comparative study of electrochemical techniques in investigating the adsorption behaviour of fibrinogen on platinum[J].Journal of Electroanalytical Chemistry,2010,649(1):206-218.
[14]王秋菊.浅析石英晶体的压电效应及应用[J].职业,2013(2):136-136.
[15]Sauerbrey G.Verwendung von Schwingquarzen zur Wgung dünner Schichten und zur Mikrowgung[J].Zeitschrift für Physik,1959,155(2):206-222.
[16]Imamura K,Shimomura M,Nagai S,et al.Adsorption characteristics of various proteins to a titanium surface[J].Journal of Bioscience and Bioengineering,2008,106(3):273-278.
[17]周笑鹏,白姝,孙彦.离子强度和溶质浓度对蛋白质在Q Sepharose FF中吸附动力学的影响[J].化工学报,2005,56(1):130-134.