从乙烷氧化富集物中分离到的乙醇氧化不动杆菌的生理生化特点
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摘要
采用floating filter方法从乙烷氧化富集物中分离出杆状菌株Z1,其最适生长温度为37℃,最适p H为6. 0,最适盐浓度为0. 2 mol/L,过氧化氢酶阳性,氧化酶阴性。其全长16S r DNA序列与Acinetobacter pittii CIP 70. 29T完全一致,可判定其为不动杆菌属(Acinetobacter)细菌。此菌株可以壬烷、十三烷为碳源生长,具有甲醇、乙醇氧化活性,却不能利用乙烷。不动杆菌菌株Z1可应用于利用floating filter方法的乙烷氧化菌的分离培养以及污染石油烃的降解。
A rod-shaped bacterial strain Z1 from ethane oxidation enrichment was isolated through a method of floating filter. The optimum growth temperature of it was 37 ℃,the optimum p H was 6. 0,the optimum salt concentration was0. 2 mol/L,the catalase was positive,and the oxidase was negative. The 16 S r DNA of strain Z1 completely matched with Acinetobacter pittii CIP 70. 29 T. Therefore,strain Z1 can be judged as a bacterium of the genus Acinetobacter.The strain can grow with nonane or tridecane as carbon source,and it can oxidize methanol and ethanol,but not ethane. The Acinetobacter sp. Z1 can be applied using to isolate ethane oxidizing bacteria through floating filter method for degradation of contaminated petroleum hydrocarbons.
A rod-shaped bacterial strain Z1 from ethane oxidation enrichment was isolated through a method of floating filter. The optimum growth temperature of it was 37 ℃,the optimum p H was 6. 0,the optimum salt concentration was0. 2 mol/L,the catalase was positive,and the oxidase was negative. The 16 S r DNA of strain Z1 completely matched with Acinetobacter pittii CIP 70. 29 T. Therefore,strain Z1 can be judged as a bacterium of the genus Acinetobacter.The strain can grow with nonane or tridecane as carbon source,and it can oxidize methanol and ethanol,but not ethane. The Acinetobacter sp. Z1 can be applied using to isolate ethane oxidizing bacteria through floating filter method for degradation of contaminated petroleum hydrocarbons.
引文
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[3]田甜,李冬梅,戴世鲲,等.海洋环境中难培养微生物的寡营养培养[J].微生物学通报,2009,36(7):1031-1039.
[4] Stackebrandt E,Embley TM. Diversity of uncultured microorganisms in the environment[M]. Nonculturable microorganisms in the environment. Springer US,2000:57-75.
[5] De Bruyn JC,Boogerd FC,Bos P,et al. Floating filters,a novel technique for isolation and enumeration of fastidious,acidophilic,iron-oxidizing,autotrophic bacteria[J]. Applied and Environmental Microbiology,1990,56(9):2891-2894.
[6] Pol A,Heijmans K,Harhangi HR,et al. Methanotrophy below pH 1 by a new Verrucomicrobia species[J]. Nature,2007,450(7171):874-878.
[7] Nguyen NL,Yu WJ,Yang HY,et al. A novel methanotroph in the genus Methylomonas that contains a distinct clade of soluble methane monooxygenase[J]. Journal of Microbiology,2017,55(10):775-782.
[8] Sheets JP,Ge X,Li YF,et al. Biological conversion of biogas to methanol using methanotrophs isolated from solid-state anaerobic digestate[J]. Bioresource Technology,2016,201:50-57.
[9] Jiang H,Duan C,Luo M,et al. Enrichment and characteristics of mixed methane-oxidizing bacteria from a Chinese coal mine[J]. Applied Microbiology and Biotechnology,2016,100(24):10331-10341.
[10] Tawthep S,Fukiya S,Lee JY,et al. Isolation of six novel 7-oxo-or urso-type secondary bile acid-producing bacteria from rat cecal contents[J]. Journal of Bioscience and Bioengineering,2017,124(5):514-522.
[11] Krizova L,Maixnerova M,Sedo O,et al. Acinetobacter albensis sp. nov.,isolated from natural soil and water ecosystems[J].International Journal of Systematic and Evolutionary Microbiology,2015,65(11):3905-3912.
[12] Dong W,Hou Y,Xi X,et al. Biodegradation of fenoxapropethyl by an enriched consortium and its proposed metabolic pathway[J]. International Biodeterioration&Biodegradation,2015,97:159-167.
[13] Beck DA,Kalyuzhnaya MG,Malfatti S,et al. A metagenomic insight into freshwater methane-utilizing communities and evidence for cooperation between the Methylococcaceae and the Methylophilaceae[J]. Peer J,2013,1(1):e23.
[14] Chistoserdova L,Kalyuzhnaya MG,Lidstrom ME. Cycling single-carbon compounds:from omics to novel concepts[J]. Microbe,2013,8:395-400.
[15]刘玉华,王慧,胡晓珂.不动杆菌属(Acinetobacter)细菌降解石油烃的研究进展[J].微生物学通报,2016,43(7):1579-1589.
[16] Doughari HJ,Ndakidemi PA,Human IS,et al. The ecology,biology and pathogenesis of Acinetobacter spp.:an overview[J]. Microbes and Environments,2011,26(2):101-112.
[17] Ron E,Rosenberg E. Acinetobacter and Alkanindiges[M].Handbook of Hydrocarbon and Lipid Microbiology. Springer Berlin Heidelberg,2010:1799-1803.
[18] Fatajeva E,Gailiūt6)e I,Paliulis D,et al. The use of Acinetobacter sp. for oil hydrocarbon degradation in saline waters[J].Biologija,2014,60(3):126-133.