四川兔源大肠埃希菌的耐药性及耐药基因检测
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摘要
从四川地区规模化家兔养殖场分离鉴定出97株大肠埃希菌(Escherichiacoli),采用Kirby–Bauer纸片法进行耐药性测定,同时采用PCR方法对相关耐药基因进行检测。结果显示:分离菌株对四环素类药物表现高度耐药,耐药率高达90.72%以上,对磺胺类药物的耐药率达72.16%,对氨基糖苷类药物的耐药率31.96%~58.76%,对多肽类的耐药率较低,为14.43%;耐药基因tet A、tet B、tet C、arm A、rmt B和flo R的检出率分别为61.86%、84.54%、16.49%、4.12%、3.09%和8.25%,未检出tet D、tet E、tet M、tet O、tet L、tet K、rmt A、rmt C、rmt D、rmt E、npm A和cfr基因。
97 Escherichia coli were isolated from rabbits farms in Sichuan and subjected to detections of antibiotics resistance and resistance genes by K–B disk diffusion and PCR. The results revealed that the isolated strains had highest resistance to tetracyclines with the rate over 90.72%, the resistance sulfonamides with the rate of 72.16%, the resistance to aminoglycoside with the rate between 31.96%–58.76%, the resistance polymyxin B with lowest rate of 14.43%. The detected genes and their rates were tetA of 61.86%, tetB of 84.54%, tetC of 16.49%, arm A of 4.12%, rmt B of 3.09% floR of 8.25%. And, no tetD, tetE, tetM, tetO, tetL, tetK, rmtA, rmtC, rmtD, rmtE, npmA, cfr was detected.
97 Escherichia coli were isolated from rabbits farms in Sichuan and subjected to detections of antibiotics resistance and resistance genes by K–B disk diffusion and PCR. The results revealed that the isolated strains had highest resistance to tetracyclines with the rate over 90.72%, the resistance sulfonamides with the rate of 72.16%, the resistance to aminoglycoside with the rate between 31.96%–58.76%, the resistance polymyxin B with lowest rate of 14.43%. The detected genes and their rates were tetA of 61.86%, tetB of 84.54%, tetC of 16.49%, arm A of 4.12%, rmt B of 3.09% floR of 8.25%. And, no tetD, tetE, tetM, tetO, tetL, tetK, rmtA, rmtC, rmtD, rmtE, npmA, cfr was detected.
引文
[1]刘书亮,张晓利,韩新锋,等.动物性食品源大肠杆菌耐药性研究[J].中国食品学报,2011,11(7):163-168.
[2]陆承平.兽医微生物学[M].4版.北京:中国农业出版社,2007:105-106.
[3]曹文革,马立红.产ESBLs革兰阴性杆菌的检测及耐药性分析[J].吉林医学,2008,9(19):1605-1606.
[4]曲志娜,胡学远,王娟,等.不同省区鸡源大肠杆菌耐药性调查与系统进化群分析[J].中国兽药杂志,2014,48(9):4-8.
[5]舒刚,侯蓉,林居纯,等.畜禽源大肠杆菌质粒介导β-内酰胺酶检测及其耐药基因特性研究[J].中国农业大学学报,2016,21(8):92-97.
[6]刘正明,李金泉,黄德浩,等.内蒙古地区羊源大肠杆菌耐药性研究[J].中国畜牧兽医,2017,44(3):839-846.
[7]坤清芳,耿毅,余泽辉,等.兔源大肠杆菌对喹诺酮药物耐药性及质粒介导的耐药基因检测[J].中国预防兽医学报,2016,38(12):944-948.
[8]M100-S24.Standards Institute.Performance standarda for antimicrobial susceptility testing;Twentieth information supplements[S].2012.
[9]陈燕杰,裴亚玲,吴华,等.鸭源性大肠杆菌氨基糖苷类耐药基因的检测与传播扩散机制[J].中国人兽共患病学报,2013,29(2):138-147.
[10]原鸿雁,郭兵方,尹晶平,等.耐药大肠杆菌氨基糖苷类修饰酶和16SrRNA甲基化酶基因的分析[J].江苏大学学报,2013,23(5):419-431.
[11]SENGELOV G,HALLING-SORENSEN B,AARE-STRUP F M.Susceptibility of Escherichia coli and Enterococcus faecium isolated from pigs and broiler chickens to tetracycline degradation products and distribution of tetracycline resistance determinants in E.coli from food animals[J].Veterinary Microbiology,2003,95(1/2):91-101.
[12]GIOVNRTTI E,BRENCIANI A,LUPID R,et al.Presence of the tet(O)gene in erythromycin-and tetracycline-resistant strains of Streptococcus pyogenes and linkage with either the mef(A)or the erm(A)gene[J].Antimicrobial Agents and Chemotherapy,2003,47(9):2844-2849.
[13]NG L K,MARTIN I,ALFA M,et al.Multiplex PCRfor the detection of tetracycline resistant genes[J].Molecular and Cellular Probes,2001,15(4):209-215.
[14]刘河冰.猪源肠球菌对氟苯尼考耐药新机制的研究[D].北京:中国农业大学,2013.
[15]代鹏飞,舒刚,林居纯,等.四川动物源大肠杆菌耐药性、产β-内酰胺酶及血清型的检测[J].中国人兽共患病学报,2016,32(4):412-416.
[16]冯敏燕,傅剑,杨世发,等.山东省鸡源大肠杆菌耐药性变化趋势监测[J].中国兽医学报,2017,37(2):250-253.
[17]岳秀英,葛荣,吴晓岚,等.四川省猪、鸡源大肠杆菌对抗生素耐药性研究[J].中国兽医杂志,2017,53(1):93-95.
[18]KOZAK G K,BOERLIN P,JANECKO N,et al.Antimicrobial resistance in Escherichia coli isolates from swine and wild small mammals in the proximity of swine farms and in natural environments in Ontario,Canada[J].Applied and Environmental Microbiology,2009,75(3):559-566.
[19]张纯萍,宁宜宝,宋立.健康鸡猪体内大肠杆菌对四环素的耐药性及耐药基因分布[J].中国人兽共患病学报,2010,43(12):2578-2583.
[20]邱军.呼伦贝尔地区猪大肠杆菌病发病情况调查及防治[D].哈尔滨:东北农业大学,2015.
[21]王爽,邓向东,闫国栋,等.食品源动物大肠杆菌耐药性分析及16S rRNA甲基化酶基因的检测[J].西北农林科技大学学报(自然科学版),2016,44(3):1-7.
[22]陈玉霞,李德喜,杜向党,等.病犬大肠杆菌16Sr RNA甲基化酶基因检测[J].中国农学通报,2011,27(17):34-37.
[23]王珊,吕媛,李耘,等.我国阿米卡星耐药大肠埃希菌16S rRNA甲基化酶基因及其水平转移研究[J].中国临床药理学杂志,2015,31(4):283-285.
[24]于学辉,李旭玲,杨发龙,等.鸭源致病性大肠杆菌耐氟苯尼考floR基因的克隆与序列分析[J].中国畜牧兽医,2010,37(3):84-86.
[25]贺志沛,王玲飞,刘建华,等.宠物犬源大肠杆菌氟苯尼考耐药基因floR的检测及药物敏感性分析[J].中国畜牧兽医,2013,40(6):211-215.
[26]李鹏.藏猪源大肠杆菌和肠球菌耐药性调查及其耐药机制的研究[D].北京:中国农业大学,2015.
[2]陆承平.兽医微生物学[M].4版.北京:中国农业出版社,2007:105-106.
[3]曹文革,马立红.产ESBLs革兰阴性杆菌的检测及耐药性分析[J].吉林医学,2008,9(19):1605-1606.
[4]曲志娜,胡学远,王娟,等.不同省区鸡源大肠杆菌耐药性调查与系统进化群分析[J].中国兽药杂志,2014,48(9):4-8.
[5]舒刚,侯蓉,林居纯,等.畜禽源大肠杆菌质粒介导β-内酰胺酶检测及其耐药基因特性研究[J].中国农业大学学报,2016,21(8):92-97.
[6]刘正明,李金泉,黄德浩,等.内蒙古地区羊源大肠杆菌耐药性研究[J].中国畜牧兽医,2017,44(3):839-846.
[7]坤清芳,耿毅,余泽辉,等.兔源大肠杆菌对喹诺酮药物耐药性及质粒介导的耐药基因检测[J].中国预防兽医学报,2016,38(12):944-948.
[8]M100-S24.Standards Institute.Performance standarda for antimicrobial susceptility testing;Twentieth information supplements[S].2012.
[9]陈燕杰,裴亚玲,吴华,等.鸭源性大肠杆菌氨基糖苷类耐药基因的检测与传播扩散机制[J].中国人兽共患病学报,2013,29(2):138-147.
[10]原鸿雁,郭兵方,尹晶平,等.耐药大肠杆菌氨基糖苷类修饰酶和16SrRNA甲基化酶基因的分析[J].江苏大学学报,2013,23(5):419-431.
[11]SENGELOV G,HALLING-SORENSEN B,AARE-STRUP F M.Susceptibility of Escherichia coli and Enterococcus faecium isolated from pigs and broiler chickens to tetracycline degradation products and distribution of tetracycline resistance determinants in E.coli from food animals[J].Veterinary Microbiology,2003,95(1/2):91-101.
[12]GIOVNRTTI E,BRENCIANI A,LUPID R,et al.Presence of the tet(O)gene in erythromycin-and tetracycline-resistant strains of Streptococcus pyogenes and linkage with either the mef(A)or the erm(A)gene[J].Antimicrobial Agents and Chemotherapy,2003,47(9):2844-2849.
[13]NG L K,MARTIN I,ALFA M,et al.Multiplex PCRfor the detection of tetracycline resistant genes[J].Molecular and Cellular Probes,2001,15(4):209-215.
[14]刘河冰.猪源肠球菌对氟苯尼考耐药新机制的研究[D].北京:中国农业大学,2013.
[15]代鹏飞,舒刚,林居纯,等.四川动物源大肠杆菌耐药性、产β-内酰胺酶及血清型的检测[J].中国人兽共患病学报,2016,32(4):412-416.
[16]冯敏燕,傅剑,杨世发,等.山东省鸡源大肠杆菌耐药性变化趋势监测[J].中国兽医学报,2017,37(2):250-253.
[17]岳秀英,葛荣,吴晓岚,等.四川省猪、鸡源大肠杆菌对抗生素耐药性研究[J].中国兽医杂志,2017,53(1):93-95.
[18]KOZAK G K,BOERLIN P,JANECKO N,et al.Antimicrobial resistance in Escherichia coli isolates from swine and wild small mammals in the proximity of swine farms and in natural environments in Ontario,Canada[J].Applied and Environmental Microbiology,2009,75(3):559-566.
[19]张纯萍,宁宜宝,宋立.健康鸡猪体内大肠杆菌对四环素的耐药性及耐药基因分布[J].中国人兽共患病学报,2010,43(12):2578-2583.
[20]邱军.呼伦贝尔地区猪大肠杆菌病发病情况调查及防治[D].哈尔滨:东北农业大学,2015.
[21]王爽,邓向东,闫国栋,等.食品源动物大肠杆菌耐药性分析及16S rRNA甲基化酶基因的检测[J].西北农林科技大学学报(自然科学版),2016,44(3):1-7.
[22]陈玉霞,李德喜,杜向党,等.病犬大肠杆菌16Sr RNA甲基化酶基因检测[J].中国农学通报,2011,27(17):34-37.
[23]王珊,吕媛,李耘,等.我国阿米卡星耐药大肠埃希菌16S rRNA甲基化酶基因及其水平转移研究[J].中国临床药理学杂志,2015,31(4):283-285.
[24]于学辉,李旭玲,杨发龙,等.鸭源致病性大肠杆菌耐氟苯尼考floR基因的克隆与序列分析[J].中国畜牧兽医,2010,37(3):84-86.
[25]贺志沛,王玲飞,刘建华,等.宠物犬源大肠杆菌氟苯尼考耐药基因floR的检测及药物敏感性分析[J].中国畜牧兽医,2013,40(6):211-215.
[26]李鹏.藏猪源大肠杆菌和肠球菌耐药性调查及其耐药机制的研究[D].北京:中国农业大学,2015.