猪源沙门氏菌多药外排泵oqx AB和氟苯尼考耐药基因floR的检测分析
|
摘要
用微量肉汤稀释法测定了215株猪源沙门氏菌对4种抗菌药物的敏感性,用PCR方法测定了多药外排泵基因oqxA、oqxB和氟苯尼考耐药基因floR,并用ERIC-PCR方法分析了猪源沙门氏菌菌株之间的相关性和遗传关系。结果显示:分离菌株对喹乙醇、痢菌净、氟苯尼考和恩诺沙星的耐药率分别为32.56%、20.93%、92.56%、16.74%;有152株菌同时携带oqxA和oqxB,另有7株菌单独携带oqxB;有156株菌携带floR基因; oqxA或oqxB阳性菌株可分为A～Q 17个ERIC型,oqxA和oqxB基因分布于不同的ERIC型菌株中。
The susceptibility of 215 swine-derived Salmonella typhimurium strains to 4 kinds of antibacterial agents was determined by using micro-broth dilution method. The multi-drug efflux pump genes oqxA,oqxB,and the florfenicol-tolerant gene floR in these S. typhimurium strains were detected by using PCR method,and the correlations and genetic relationships among these strains were analyzed by using ERIC-PCR method. The results showed that the tolerant rate of S. typhimurium isolates to olaquindox,mequindox,florfenicol and enrofloxacin was 32.56%,20.93%,92.56% and 16.74%,respectively. A total of 152 strains carried both oqxA and oqxB,and seven strains only carried oqxB. In addition,there were 156 strains carrying the gene floR. The oqxA or oqxB positive strains could be divided into 17 ERIC types( A ～ Q type),and the genes oqxA and oqxB were widely distributed in different ERIC types of strains.
The susceptibility of 215 swine-derived Salmonella typhimurium strains to 4 kinds of antibacterial agents was determined by using micro-broth dilution method. The multi-drug efflux pump genes oqxA,oqxB,and the florfenicol-tolerant gene floR in these S. typhimurium strains were detected by using PCR method,and the correlations and genetic relationships among these strains were analyzed by using ERIC-PCR method. The results showed that the tolerant rate of S. typhimurium isolates to olaquindox,mequindox,florfenicol and enrofloxacin was 32.56%,20.93%,92.56% and 16.74%,respectively. A total of 152 strains carried both oqxA and oqxB,and seven strains only carried oqxB. In addition,there were 156 strains carrying the gene floR. The oqxA or oqxB positive strains could be divided into 17 ERIC types( A ～ Q type),and the genes oqxA and oqxB were widely distributed in different ERIC types of strains.
引文
[1]Adewoye L,Topp E,Li X Z. Antimicrobial drug efflux genes and pumps in bacteria of animal and environmental origin[M]. Springer International Publishing,2016.
[2]Sorensen A H,Hansen L H,Johannesen E,et al. Conjugative plasmid conferring resistance to olaquindox[J]. Antimicrobial Agents and Chemotherapy,2003,47(2):798-799.
[3]Hansen L H,Jensen L B,Srensen S J,et al. The prevalence of the OqxAB multidrug efflux pump amongst olaquindox-resistant Escherichia coli in pigs[J]. Microbial Drug Resistance,2005,11(4):378.
[4]Rodríguezmartínez J M,Alba P D D,Briales A,et al. Contribution of OqxAB efflux pumps to quinolone resistance in extended-spectrum-β-lactamase-producing Klebsiella pneumoniae[J]. Journal of Antimicrobial Chemotherapy,2013,68(1):68-73.
[5]Hansen L H,Jensen L B,Srensen H I,et al. Substrate specificity of the OqxAB multidrug resistance pump in Escherichia coli and selected enteric bacteria[J]. J Antimicrob Chemother,2007,60(1):145-147.
[6]Zhao J,Chen Z,Chen S,et al. Prevalence and dissemination of oqxAB in Escherichia coli isolates from animals,farmworkers,and the environment[J]. Antimicrobial Agents&Chemotherapy,2010,54(10):4219.
[7]Zhong X,Xu H,Chen D,et al. First emergence of acrAB and oqxAB mediated tigecycline resistance in clinical isolates of Klebsiella pneumoniae predating the use of tigecycline in a Chinese hospital[J]. Plos One,2014,9(12):e115185.
[8]Kim E,Aoki T. Sequence analysis of the florfenicol resistance gene encoded in the transferable R-plasmid of a fish pathogen,Pasteurella piscicida[J]. Microbiology&Immunology,1996,40(9):665-669.
[9]Park K S,Kim M H,Park T S,et al. Prevalence of the plasmid-mediated quinolone resistance genes,aac(6')-Ib-cr,qepA,and oqxAB in clinical isolates of extended-spectrumβ-lactamase(ESBL)-producing Escherichia coli and Klebsiella pneumoniae in Korea[J]. Annals of Clinical&Laboratory Science,2012,42(2):191-197.
[10]Cheng L,Cao X L,Zhang Z F,et al. Clonal dissemination of KPC-2 producing Klebsiella pneumoniae ST11 clone with high prevalence of oqxAB and rmtB in a tertiary hospital in China:results from a 3-year period[J]. Annals of Clinical Microbiology&Antimicrobials,2016,15(1):1.
[11]Perez F,Rudin S D,Marshall S H,et al. OqxAB,a quinolone and olaquindox efflux pump,is widely distributed among multidrug-resistant Klebsiella pneumoniae isolates of human origin[J]. Antimicrobial Agents&Chemotherapy,2013,57(9):4602-4603.
[12]Li L,Liao X,Yang Y,et al. Spread of oqxAB in Salmonella enterica serotype typhimurium predominantly by IncHI2 plasmids[J]. Journal of Antimicrobial Chemotherapy,2013,68(10):2263-2268.
[13] CLSI. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals:approved standard[M]. Clinical and Laboratory Standards Institute,2013.
[14]王傲雪,张凌云,张桉潮,等.肉鸡沙门氏菌的分离鉴定及药敏试验[J].中国畜牧兽医,2013,40(7):167-171.
[15]狄文婷,杜雄伟,吴静,等.猪源沙门氏菌的分离与耐药性分析[J].江苏农业科学,2014,42(10):2286-2289.
[16]黄凯,陈素娟,黄骏,等.动物源性沙门氏菌的耐药性分析及氟苯尼考类耐药基因的鉴定[J].中国畜牧兽医,2015,42(2):459-466.
[17]刘艳红,李颖,吕淑霞.猪源沙门菌和大肠杆菌耐药性分析[J].畜牧与兽医,2015,47(12):160-161.
[18]宫艳杉.山东地区死亡鸭胚中细菌分离鉴定及沙门氏菌耐药性与毒力基因检测[D].泰安:山东农业大学,2017.
[19]江萍,关茹飞,夏利宁,等.新疆猪源沙门氏菌耐药性及耐药基因检测[J].中国畜牧兽医,2017,44(3):896-903.
[2]Sorensen A H,Hansen L H,Johannesen E,et al. Conjugative plasmid conferring resistance to olaquindox[J]. Antimicrobial Agents and Chemotherapy,2003,47(2):798-799.
[3]Hansen L H,Jensen L B,Srensen S J,et al. The prevalence of the OqxAB multidrug efflux pump amongst olaquindox-resistant Escherichia coli in pigs[J]. Microbial Drug Resistance,2005,11(4):378.
[4]Rodríguezmartínez J M,Alba P D D,Briales A,et al. Contribution of OqxAB efflux pumps to quinolone resistance in extended-spectrum-β-lactamase-producing Klebsiella pneumoniae[J]. Journal of Antimicrobial Chemotherapy,2013,68(1):68-73.
[5]Hansen L H,Jensen L B,Srensen H I,et al. Substrate specificity of the OqxAB multidrug resistance pump in Escherichia coli and selected enteric bacteria[J]. J Antimicrob Chemother,2007,60(1):145-147.
[6]Zhao J,Chen Z,Chen S,et al. Prevalence and dissemination of oqxAB in Escherichia coli isolates from animals,farmworkers,and the environment[J]. Antimicrobial Agents&Chemotherapy,2010,54(10):4219.
[7]Zhong X,Xu H,Chen D,et al. First emergence of acrAB and oqxAB mediated tigecycline resistance in clinical isolates of Klebsiella pneumoniae predating the use of tigecycline in a Chinese hospital[J]. Plos One,2014,9(12):e115185.
[8]Kim E,Aoki T. Sequence analysis of the florfenicol resistance gene encoded in the transferable R-plasmid of a fish pathogen,Pasteurella piscicida[J]. Microbiology&Immunology,1996,40(9):665-669.
[9]Park K S,Kim M H,Park T S,et al. Prevalence of the plasmid-mediated quinolone resistance genes,aac(6')-Ib-cr,qepA,and oqxAB in clinical isolates of extended-spectrumβ-lactamase(ESBL)-producing Escherichia coli and Klebsiella pneumoniae in Korea[J]. Annals of Clinical&Laboratory Science,2012,42(2):191-197.
[10]Cheng L,Cao X L,Zhang Z F,et al. Clonal dissemination of KPC-2 producing Klebsiella pneumoniae ST11 clone with high prevalence of oqxAB and rmtB in a tertiary hospital in China:results from a 3-year period[J]. Annals of Clinical Microbiology&Antimicrobials,2016,15(1):1.
[11]Perez F,Rudin S D,Marshall S H,et al. OqxAB,a quinolone and olaquindox efflux pump,is widely distributed among multidrug-resistant Klebsiella pneumoniae isolates of human origin[J]. Antimicrobial Agents&Chemotherapy,2013,57(9):4602-4603.
[12]Li L,Liao X,Yang Y,et al. Spread of oqxAB in Salmonella enterica serotype typhimurium predominantly by IncHI2 plasmids[J]. Journal of Antimicrobial Chemotherapy,2013,68(10):2263-2268.
[13] CLSI. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals:approved standard[M]. Clinical and Laboratory Standards Institute,2013.
[14]王傲雪,张凌云,张桉潮,等.肉鸡沙门氏菌的分离鉴定及药敏试验[J].中国畜牧兽医,2013,40(7):167-171.
[15]狄文婷,杜雄伟,吴静,等.猪源沙门氏菌的分离与耐药性分析[J].江苏农业科学,2014,42(10):2286-2289.
[16]黄凯,陈素娟,黄骏,等.动物源性沙门氏菌的耐药性分析及氟苯尼考类耐药基因的鉴定[J].中国畜牧兽医,2015,42(2):459-466.
[17]刘艳红,李颖,吕淑霞.猪源沙门菌和大肠杆菌耐药性分析[J].畜牧与兽医,2015,47(12):160-161.
[18]宫艳杉.山东地区死亡鸭胚中细菌分离鉴定及沙门氏菌耐药性与毒力基因检测[D].泰安:山东农业大学,2017.
[19]江萍,关茹飞,夏利宁,等.新疆猪源沙门氏菌耐药性及耐药基因检测[J].中国畜牧兽医,2017,44(3):896-903.