2017-2018年吉林部分地区规模化猪场猪细环病毒的遗传变异分析
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摘要
为了解猪细环病毒(TTSuVs)在吉林部分规模化猪场的遗传变异情况,对2017-2018年来自吉林部分地区的10家规模化猪场的574份血清样品进行TTSuVs流行情况调查,并根据收集地点对20株TTSuVs进行ORF1全基因序列扩增及遗传变异性分析。结果显示:10家规模化猪场中,TTSuV1感染率为36. 75%,TTSuV2感染率为63. 2%,TTSuV1与TTSuV2混合感染率为30. 3%。10株TTSuV1测序株之间的核苷酸同源性为75. 7%～94. 7%,氨基酸同源性为48. 1%～88. 2%;10株TTSuV2测序株之间的核苷酸同源性为77.5%～94.0%,氨基酸同源性为70.6%～87.4%。研究表明:吉林地区部分规模化猪场流行的TTSuV1均属于TTSuV1-lb型,而TTSuV2流行情况较为复杂,各种亚型均有涉及且呈现遗传变异多样性。
In order to study the genetic variation of Torque teno sus virus( TTSuVs) in large-scale pig farms in Jilin,574 serum samples from 10 large-scale pig farms in Jilin from 2017 to 2018 were tested for TTSuVs.According to the collection farms,the genetic variability of ORF1 gene sequence of 20 strains was analyzed. The results showed that the rate of TTSuV1 infection was 36.75%,the rate of TTSuV2 infection rate was 63.2%,and the mixed rate of TTSuV1 and TTSuV2 infection rate was 30.3%. The nucleotide homology of 10 TTSuV1 strains was 75.7% ～ 94.7%,amino acid homology was 48.1% ～ 88.2%. The nucleotide homology of 10 TTSuV2 strains was 77.5% ～ 94.0%,amino acid homology was 70.6% ～ 87.4%. The TTSuV1 in some large-scale pig farms in Jilin belongs to TTSuV1-lb. The TTSuV2 epidemic situation was complex,various subtypes were involved,and showed genetic diversity.
In order to study the genetic variation of Torque teno sus virus( TTSuVs) in large-scale pig farms in Jilin,574 serum samples from 10 large-scale pig farms in Jilin from 2017 to 2018 were tested for TTSuVs.According to the collection farms,the genetic variability of ORF1 gene sequence of 20 strains was analyzed. The results showed that the rate of TTSuV1 infection was 36.75%,the rate of TTSuV2 infection rate was 63.2%,and the mixed rate of TTSuV1 and TTSuV2 infection rate was 30.3%. The nucleotide homology of 10 TTSuV1 strains was 75.7% ～ 94.7%,amino acid homology was 48.1% ～ 88.2%. The nucleotide homology of 10 TTSuV2 strains was 77.5% ～ 94.0%,amino acid homology was 70.6% ～ 87.4%. The TTSuV1 in some large-scale pig farms in Jilin belongs to TTSuV1-lb. The TTSuV2 epidemic situation was complex,various subtypes were involved,and showed genetic diversity.
引文
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[17]Luo K,Liang W,He H,et al.Experimental infection of nonenveloped DNA virus(TTV)in Rhesus Monkey[J].Journal of Medical Virology,2015,61(1):159-164.
[2]Grenier M,Jauslin H R,Klein C,et al.Prevalence of Torque teno virus infection in pig herds in China[J].Veterinary Record,2011,168(15):410.
[3]Cortey M,Macera L,Segalés J,et al.Genetic variability and phylogeny of Torque teno sus virus 1(TTSu V1)and 2(TTSu V2)based on complete genomes[J].Veterinary Microbiology,2011,148(2):125-131.
[4]Cruz A C D M,Silveira R L,Baez C F,et al.Clinical aspects and weight gain reduction in swine infected with Porcine Circovirus type 2 and Torque Teno Sus Virus in Brazil[J].Veterinary Microbiology,2016,195:154-157.
[5]阮文强,宝志鹏,覃思楠,等.四川地区猪细环病毒1型的流行病学调查和分子特症研究[J].动物医学进展,2018,39(3):19-23.Ruan W Q,Bao Z P,Qin S N,et al.Molecular epidemiology and molecular characterization of TTSuV1 in Sichuan province[J].Progress in Veterinary Medicine,2018,39(3):19-23.
[6]Li Z,Qiao J,He Y,et al.Analysis of TTSu V1b antibody in porcine serum and its correlation with four antibodies against common viral infectious diseases[J].Virology Journal,2015,12(1):125.
[7]陈鹏强,胡崇伟,林群群,等.猪细环病毒1a型ORF1基因的克隆与序列分析[J].中国畜牧兽医,2015,42(7):1668-1673.Chen P Q,Hu C W,Lin Q Q,et al.Cloning and sequence analysis of ORF1 gene of porcine Torque Teno Sus Virus type 1a[J].China Animal Husbandry&Veterinary Medicine,2015,42(7):1668-1673.
[8]Luka P D,Erume J,Yakubu B,et al.Molecular Detection of Torque teno sus virus and coinfection with African swine fever virus in blood samples of pigs from some slaughterhouses in Nigeria[J].Advances in Virology,2016,6:6341015.
[9]Teixeira T F,Cibulski S P,Santos H F D,et al.Torque teno sus virus 1(TTSuV1)and 2(TTSuV2)viral loads in serum of postweaning multisystemic wasting syndrome(PMWS)-affected and healthy pigs in Brazil[J].Research in Veterinary Science,2015,101:38-41.
[10]Lee Y,Lin C M,Jeng C R,et al.The pathogenic role of Torque teno sus virus 1 and 2 and their correlations with various viral pathogens and host immunocytes in wasting pigs[J].Veterinary Microbiology,2015,180(3):186-195.
[11]Singh G,Ramamoorthy S.Potential for the cross-species transmission of swine Torque teno viruses[J].Veterinary Microbiology,2018,215:66-77.
[12]Hamilton G.Virology:The gene weavers[J].Nature,2006,441(7094):683-685.
[13]王礞礞.猪源TTV:分子流行病学,全长序列分析及感染性分子克隆的构建[D].中国农业科学院,2011.Wang M M.Porcine TTV:molecular epidemiology,full length genomic sequence analysis and construction of infectious molecular clones[D].Chinese Academy of Agricultural Sciences,2011.
[14]Peng Y H,Nishizawa T,Takahashi M,et al.Analysis of the entire genomes of thirteen TT virus variants classifiable into the fourth and fifth genetic groups,isolated from viremic infants[J].Archives of Virology,2002,147(1):21-41.
[15]陈轶雯.广东TTSuVs流行病学调查与TTSuV_2 ORF1抗原性分析[D].南京农业大学,2013.Chen Y W.Epidemiological investigation in Guangdong for Porcine Transfusion Transmitted Virus and immunogenicity research for TTSuV2 ORF1[D].Nanjing Agricultural University,2013.
[16]Panichi F,Goz'dziewski K,Migaszewski C,et al.Kepler-30:the Cesar of TTV variations[J].Monthly Notices of the Royal Astronomical Society,2018,478(2):2480-2494.
[17]Luo K,Liang W,He H,et al.Experimental infection of nonenveloped DNA virus(TTV)in Rhesus Monkey[J].Journal of Medical Virology,2015,61(1):159-164.