摘要
为了解猪细环病毒(TTSuVs)在吉林部分规模化猪场的遗传变异情况,对2017-2018年来自吉林部分地区的10家规模化猪场的574份血清样品进行TTSuVs流行情况调查,并根据收集地点对20株TTSuVs进行ORF1全基因序列扩增及遗传变异性分析。结果显示:10家规模化猪场中,TTSuV1感染率为36. 75%,TTSuV2感染率为63. 2%,TTSuV1与TTSuV2混合感染率为30. 3%。10株TTSuV1测序株之间的核苷酸同源性为75. 7%~94. 7%,氨基酸同源性为48. 1%~88. 2%;10株TTSuV2测序株之间的核苷酸同源性为77.5%~94.0%,氨基酸同源性为70.6%~87.4%。研究表明:吉林地区部分规模化猪场流行的TTSuV1均属于TTSuV1-lb型,而TTSuV2流行情况较为复杂,各种亚型均有涉及且呈现遗传变异多样性。
In order to study the genetic variation of Torque teno sus virus( TTSuVs) in large-scale pig farms in Jilin,574 serum samples from 10 large-scale pig farms in Jilin from 2017 to 2018 were tested for TTSuVs.According to the collection farms,the genetic variability of ORF1 gene sequence of 20 strains was analyzed. The results showed that the rate of TTSuV1 infection was 36.75%,the rate of TTSuV2 infection rate was 63.2%,and the mixed rate of TTSuV1 and TTSuV2 infection rate was 30.3%. The nucleotide homology of 10 TTSuV1 strains was 75.7% ~ 94.7%,amino acid homology was 48.1% ~ 88.2%. The nucleotide homology of 10 TTSuV2 strains was 77.5% ~ 94.0%,amino acid homology was 70.6% ~ 87.4%. The TTSuV1 in some large-scale pig farms in Jilin belongs to TTSuV1-lb. The TTSuV2 epidemic situation was complex,various subtypes were involved,and showed genetic diversity.
引文
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