卵泡液FOXL2与卵巢反应的相关性
|
摘要
目的探讨卵泡液FOXL2在超促排卵周期中与卵巢对外源性促性腺激素(Gn)反应的相关性。方法将231例长方案超促排卵周期治疗的不孕患者按照Gn用量分为4组,A组(<2 000 IU)、B组(2 000~2999 IU)、C组(3 001~3 999 IU)、D组(≥4 000 IU)。收集患者卵泡液采用ELISA双抗体夹心法检测FOXL2的浓度。结果随着Gn用量的增加,卵泡液FOXL2表达逐渐降低,组间差异有统计学意义(P<0.05)。各组间Gn用量越高,患者优质胚胎率越低,差异有统计学意义(P<0.05)。临床妊娠率随Gn用量增加呈下降趋势,由于样本量限制,差异无统计学意义。结论超促排卵周期卵泡液FOXL2水平与卵巢反应性呈正相关。高水平的FOXL2可能预示着更好的临床结局。
Objective To investigate the correlation between FOXL2 level in follicular fluid and ovary response to exogenous gonadotropins( Gn) during controlled ovarian hyperstimulation. Methods Totally 231 infertile patients,who received the treatment of controlled ovarian stimulation( COH),were divided into four groups based on the dose of exogenouse gonadotropins,Group A( < 2 000 IU),Group B( 2 000-2 999 IU),Group C( 3 000-3 999 IU) and Group D( ≥4 000 IU). Follicular fluid( FF) were collected during oocytes retrieval. The levels of FOXL2 in FF samples were assessed by double-antibody sandwich ELISA. Results FOXL2 level in follicular was significantly positively correlated with Gn dose( P < 0. 05). Among these four groups,significantly higher live birth rate and high-quality embryonic rate were obtained in lower Gn dose group( P < 0. 05). Conclusion FOXL2 in FF is good marker to predict the ovarian response and the COH outcome.
Objective To investigate the correlation between FOXL2 level in follicular fluid and ovary response to exogenous gonadotropins( Gn) during controlled ovarian hyperstimulation. Methods Totally 231 infertile patients,who received the treatment of controlled ovarian stimulation( COH),were divided into four groups based on the dose of exogenouse gonadotropins,Group A( < 2 000 IU),Group B( 2 000-2 999 IU),Group C( 3 000-3 999 IU) and Group D( ≥4 000 IU). Follicular fluid( FF) were collected during oocytes retrieval. The levels of FOXL2 in FF samples were assessed by double-antibody sandwich ELISA. Results FOXL2 level in follicular was significantly positively correlated with Gn dose( P < 0. 05). Among these four groups,significantly higher live birth rate and high-quality embryonic rate were obtained in lower Gn dose group( P < 0. 05). Conclusion FOXL2 in FF is good marker to predict the ovarian response and the COH outcome.
引文
[1]Caburet S,Georges A,L'Hote A,et al.The transcription factor FOXL2:at the crossroads of ovarian physiology and pathology[J].Mol Cell Endocrinol,2012,356(1):55-64.
[2]Bellessort B,Bachelot A,Heude E,et al.Role fo Foxl2 in uterine maturation and fuction[J].Human Molecular Genetics,2015,24(11):3092-3103.
[3]Cocquet J,Pailhoux E,Jaubert F,et al.Evolution and expression of FOXL2[J].J Med Genet,2002,39(9):916-922.
[4]曲中玉,陈子江.FOXL2——不孕有关的转录因子[J].生殖与避孕,2006,26(1):40-43.
[5]胡琳莉,孙莹璞.卵巢储备功能与卵巢反应性评估[J].中国实用妇科与产科杂志,2015,31(1):18-21.
[6]Valerie LB,Morton BB,Barbara L,et al.Gonadotropin dose is negatively correlated with live birth rate:analysis of over 650000ART cycles[J].Fertil Steril,2015,104(5):1145-1152.
[7]吕芳,张保合,陈智英.IVF-ET患者IGFBP-3和IGF-1水平与卵泡发育、卵巢内分泌、胚胎质量及妊娠之间的关系[J].广东医学,2016,37(12):1836-1839.
[8]龚绍琼.GnRHa长方案中促性腺激素用量对体外受精胚胎移植结局的影响[J].实用医学杂志,2012,28(25):2537-2539.
[9]刘寒艳,靳秀云,刘见桥,等.增加启动剂量对年龄相关的卵巢反应性以及临床结局的影响[J].广东医学,2017,38(2):291-295.
[10]胡琳莉,孙莹璞.卵巢储备功能与卵巢反应性评估[J].中国实用妇科与产科杂志,2015,31(1):18-21.
[11]Anne ZS,David P,Frank ZS,et al.Association between biomarkers of ovarian reserve and infertility among older women of reproductive age[J].JAMA,2017,318(14):1367-1376.
[12]Sandro S,Federica M,Susanna X,et al.The anti-müllerian hormone(AMH)induces forkhead box L2(FOXL2)expression in primary culture of human granulose cells in vitro[J].J Assist Reprod Genet,2017,34(9):1131-1136.
[13]Yufeng S,Yuxia D,Feng H,et al.DNA methylation level of cyp19a1a and Foxl2 gene related to their expression patterns and reproduction traits during ovary development stages of Japanese flounder(paralichthy olivaceus)[J].Gene,2016,575(2):321-330.
[14]曲中玉,陈子江.FOXL2对IVF周期卵巢反应性的影响[J].现代妇产科进展,2006,15(2):128-130.
[15]Jin H,Won M,Park SE,et al.FOXL2 is an essential activator of SF-1-induced transcriptional regulation of anti-mullerian hormone in human granulose cells[J].PLo S One,2016,11(7):e0159112.
[2]Bellessort B,Bachelot A,Heude E,et al.Role fo Foxl2 in uterine maturation and fuction[J].Human Molecular Genetics,2015,24(11):3092-3103.
[3]Cocquet J,Pailhoux E,Jaubert F,et al.Evolution and expression of FOXL2[J].J Med Genet,2002,39(9):916-922.
[4]曲中玉,陈子江.FOXL2——不孕有关的转录因子[J].生殖与避孕,2006,26(1):40-43.
[5]胡琳莉,孙莹璞.卵巢储备功能与卵巢反应性评估[J].中国实用妇科与产科杂志,2015,31(1):18-21.
[6]Valerie LB,Morton BB,Barbara L,et al.Gonadotropin dose is negatively correlated with live birth rate:analysis of over 650000ART cycles[J].Fertil Steril,2015,104(5):1145-1152.
[7]吕芳,张保合,陈智英.IVF-ET患者IGFBP-3和IGF-1水平与卵泡发育、卵巢内分泌、胚胎质量及妊娠之间的关系[J].广东医学,2016,37(12):1836-1839.
[8]龚绍琼.GnRHa长方案中促性腺激素用量对体外受精胚胎移植结局的影响[J].实用医学杂志,2012,28(25):2537-2539.
[9]刘寒艳,靳秀云,刘见桥,等.增加启动剂量对年龄相关的卵巢反应性以及临床结局的影响[J].广东医学,2017,38(2):291-295.
[10]胡琳莉,孙莹璞.卵巢储备功能与卵巢反应性评估[J].中国实用妇科与产科杂志,2015,31(1):18-21.
[11]Anne ZS,David P,Frank ZS,et al.Association between biomarkers of ovarian reserve and infertility among older women of reproductive age[J].JAMA,2017,318(14):1367-1376.
[12]Sandro S,Federica M,Susanna X,et al.The anti-müllerian hormone(AMH)induces forkhead box L2(FOXL2)expression in primary culture of human granulose cells in vitro[J].J Assist Reprod Genet,2017,34(9):1131-1136.
[13]Yufeng S,Yuxia D,Feng H,et al.DNA methylation level of cyp19a1a and Foxl2 gene related to their expression patterns and reproduction traits during ovary development stages of Japanese flounder(paralichthy olivaceus)[J].Gene,2016,575(2):321-330.
[14]曲中玉,陈子江.FOXL2对IVF周期卵巢反应性的影响[J].现代妇产科进展,2006,15(2):128-130.
[15]Jin H,Won M,Park SE,et al.FOXL2 is an essential activator of SF-1-induced transcriptional regulation of anti-mullerian hormone in human granulose cells[J].PLo S One,2016,11(7):e0159112.