SFRP5基因遗传变异对长顺绿壳蛋鸡胸肌肌肉品质的影响
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摘要
分泌型卷曲相关蛋白5(secreted frizzled related protein 5, SFRP5)通过Wnt信号通路调节脂质代谢,改善胰岛素抵抗,维持炎症平衡,是白色脂肪组织分泌的抗炎症脂肪因子之一。为了探讨SFRP5基因多态性与长顺绿壳蛋鸡(Gallus gallus domesticus)肌肉品质的关系、寻找与蛋鸡肌肉品质的相关分子标记、本研究采用直接测序法检测SFRP5基因外显子的单核苷酸多态,分析其对长顺绿壳蛋鸡胸肌肌肉品质的遗传效应。结果表明:在SFRP5基因外显子3上共检测到2个突变位点,g.23253254 G>A的优势基因型和优势等位基因为GG基因型和G等位基因,频率分别为0.569和0.756;g.23253269 T>G的优势基因型和优势等位基因为TT基因型和T等位基因,频率分别为0.681和0.812,且2个位点的突变并未引起氨基酸的变化,为同义突变。2个多态位点的多态信息含量(PIC)均处于0.25～0.5之间,属于中度多态位点,卡方(χ2)检测显示:2个突变位点的基因型分布均未偏离Hardy-Weinberg平衡。连锁不平衡分析发现,SFRP5基因的2个突变位点间均不存在强连锁不平衡。2个SNPs位点在长顺绿壳蛋鸡群体中发现了3种单倍型H1、H2和H3,其频率分别为0.569、0.187和0.244;6种双倍型H1H1、H1H2、H1H3、H2H2、H2H3和H3H3,频率分别为0.313、0.200、0.313、0.056、0.063和0.056。关联分析表明,SFRP5基因的g.23253254 G>A位点对胸肌粗脂肪含量有显著影响(P<0.05);2个SNPs位点组成的双倍型中,双倍型H3H3(AATT)对胸肌粗脂肪含量的影响达到显著水平(P<0.05),显著高于其他5种双倍型(P<0.05),揭示SFRP5基因的遗传变异能影响长顺绿壳蛋鸡胸肌脂肪沉积,g.23253254 G>A和g.23253269 T>G变异位点可能作为提高长顺绿壳蛋鸡胸肌脂肪的分子标记。本研究为进一步探明SFRP5基因变异调控家禽肌肉品质的分子机制提供了理论依据。
Secreted frizzled-related protein 5(SFRP5) is a crucial anti-inflammatory adipokine secreted from white adipose tissue. It can regulate lipid metabolism, reduce insulin resistance, and keep inflammation balance by Wnt singal pathway. To investigate the relationship between SFRP5 gene polymorphism and muscle quality in Changshun Blue-eggshell chicken(Gallus gallus domesticus), and find molecular markers related to muscle quality. In this study, the single nucleotide polymorphisms(SNPs) of SFRP5 gene were detected by direct sequencing, and its correlation with the meat quality of chest muscle in Changshun Blueeggshell chicken was analyzed. The results showed that 2 SNPs(g.23253254 G>A and g.23253269 T>G) were firstly found in SFRP5 gene exon 3. The dominant genotype and dominant allele of the g.23253254 G>A locus was GG genotype and G allele, and its frequencies were 0.569 and 0.756, respectively. The g.23253269 T>G locus was TT genotype and T allele with frequencies were 0.681 and 0.812, respectively. Two mutation sites of SFRP5 gene were synonymous mutations. Polymorphic information content(PIC) of the 2 loci were between0.25 and 0.5, and belong to moderate polymorphic loci. The chi-square test indicated that the genotypes distribution of 2 SNPs(g. 23253254 G>A and g. 23253269 T>G) were agree with the Hardy-Weinberg equilibrium(P>0.05). There was no strong linkage disequilibrium(LD) between each SNP. Among the flock of Changshun Blue-eggshell chicken, 3 haplotypes and 6 diplotypes were formed on SFRP5 gene. The frequency of 3 haplotypes H1, H2 and H3 were 0.569, 0.187 and 0.244 respectively, and then the frequency of6 diplotypes H1 H1, H1 H2, H1 H3, H2 H2, H2 H3 and H3 H3 were 0.313, 0.200, 0.313, 0.056, 0.063 and 0.056,respectively. Genetic correlation analysis demonstated that g.23253254 G>A locus was significantly correlated with crude fat of chest muscle(P<0.05), and further the diplotype H3 H3 was obviously higher crude fat content than other 5 diplotypes(P<0.05). The present research revealed that SFRP5 gene had significant genotype effects on chicken meat quality and also suggested that both g.23253254 G>A and g.23253269 T>G variant sites of SFRP5 gene may be used as molecular markers to improve meat quality in Changshun Blueeggshell chicken. This study provide theoretical basis for further exploring the molecular mechanism of SFRP5 gene variation in poultry muscle quality.
Secreted frizzled-related protein 5(SFRP5) is a crucial anti-inflammatory adipokine secreted from white adipose tissue. It can regulate lipid metabolism, reduce insulin resistance, and keep inflammation balance by Wnt singal pathway. To investigate the relationship between SFRP5 gene polymorphism and muscle quality in Changshun Blue-eggshell chicken(Gallus gallus domesticus), and find molecular markers related to muscle quality. In this study, the single nucleotide polymorphisms(SNPs) of SFRP5 gene were detected by direct sequencing, and its correlation with the meat quality of chest muscle in Changshun Blueeggshell chicken was analyzed. The results showed that 2 SNPs(g.23253254 G>A and g.23253269 T>G) were firstly found in SFRP5 gene exon 3. The dominant genotype and dominant allele of the g.23253254 G>A locus was GG genotype and G allele, and its frequencies were 0.569 and 0.756, respectively. The g.23253269 T>G locus was TT genotype and T allele with frequencies were 0.681 and 0.812, respectively. Two mutation sites of SFRP5 gene were synonymous mutations. Polymorphic information content(PIC) of the 2 loci were between0.25 and 0.5, and belong to moderate polymorphic loci. The chi-square test indicated that the genotypes distribution of 2 SNPs(g. 23253254 G>A and g. 23253269 T>G) were agree with the Hardy-Weinberg equilibrium(P>0.05). There was no strong linkage disequilibrium(LD) between each SNP. Among the flock of Changshun Blue-eggshell chicken, 3 haplotypes and 6 diplotypes were formed on SFRP5 gene. The frequency of 3 haplotypes H1, H2 and H3 were 0.569, 0.187 and 0.244 respectively, and then the frequency of6 diplotypes H1 H1, H1 H2, H1 H3, H2 H2, H2 H3 and H3 H3 were 0.313, 0.200, 0.313, 0.056, 0.063 and 0.056,respectively. Genetic correlation analysis demonstated that g.23253254 G>A locus was significantly correlated with crude fat of chest muscle(P<0.05), and further the diplotype H3 H3 was obviously higher crude fat content than other 5 diplotypes(P<0.05). The present research revealed that SFRP5 gene had significant genotype effects on chicken meat quality and also suggested that both g.23253254 G>A and g.23253269 T>G variant sites of SFRP5 gene may be used as molecular markers to improve meat quality in Changshun Blueeggshell chicken. This study provide theoretical basis for further exploring the molecular mechanism of SFRP5 gene variation in poultry muscle quality.
引文
白冰,吴新民,郭亚明,等.2015.分泌型卷曲相关蛋白5通过调控Wnt信号通路对代谢性疾病发病的影响[J].兰州大学学报(医学版),41(1):14-17.(Bai B,Wu X M,Guo Y M,et al.2015.Secreted frizzled-related protein 5plays a role in pathogenesis of metabolic disease through regulating Wnt signaling pathways[J].Journal of Lanzhou University(Medical Sciences),41(1):14-17.)
卜小雁,李辉,施晓丽,等.2017.70周龄长顺绿壳蛋鸡HO-1基因在不同组织中表达差异研究[J].中国畜牧兽医,44(06):1762-1768.(Bu X Y,Li H,Shi X L,et al.2017.Study on different expression of HO-1 gene of 70-weeks-old changshun blue-shelled chicken in different tissues[J].China Animal Husbandry&Veterinary Medicine,44(6):1762-1768.)
崔焕先.2011.肉鸡肌内脂肪形成的分子调控网络及相关基因研究[D].博士学位论文,中国农业科学院,导师:文杰.pp.1-5.(Cui H Y.2011.The research on molecular regulatory network and gene related intramuscular fat deposition in chicken[D].Thesis for Ph.D.,Chinese Academy of Agricultural Sciences,Supervisor:Wen J.pp.1-5.)
付守艺,闫峰宾,陈毅,等.2018.鸡miR-15a对胸肌肌内脂肪沉积的调控作用[J].畜牧与兽医,50(4):60-65.(Fu S Y,Yan F B,Chen Y,et al.2018.The function of miR-15a in regulating intramuscular fat deposition in pectoralis muscle in chicken[J].Animal Husbandry&Veterinary Medicine,50(4):60-65.)
李晓存,户国,王守志,等.2011.鸡脂肪性状重要候选基因的连锁不平衡模式分析[J].农业生物技术学报,19(2):278-286.(Li X C,Hu G,Wang S Z,et al.2011.Linkage disequilibrium patterns of polymorphisms in candidate genes for fatness in chickens[J].Journal of Agricultural Biotechnology,19(2):278-286.)
刘榜.2007.家畜育种学[M].北京:中国农业出版社,pp.241-248.(Liu B.2007.Animal Breeding[M].Beijin:China Agricultural Press,pp.241-248.)
吕慈.2012.脂肪因子Sfrp5与肥胖及其相关代谢紊乱的相关性研究[D].硕士学位论文,第三军医大学,导师:陈兵.pp.9-26.(Lv C.2012.The relationship of adipokine SFRP5 with obesity and obesity-linked metabolic disorders[D].Master's degree thesis,Third Military Medical University,Supervisor:Chen B,pp.9-26.)
农业部种猪质量监督检验测试中心,2004,NY/T 821-2004,猪肌肉品质测定技术规范[S],北京:中国标准出版社出版,pp.2-6.(Ministry of agriculture breeding pig quality supervision,inspection and testing center,2005,Technical specification for determination of pig muscle quality[M],Beijing:Published by China standard press,pp.2-6).
田茗源.2016.中枢输注Sfrp5对高脂诱导下丘脑炎症大鼠肝脏VLDL-TG的分泌调控及其机制[D].博士学位论文,重庆医科大学,导师:杨刚毅.pp.24-38.(Tian MY.2016.Secretion regulation and mechanism of central infusion of Sfrp5 on VLDL-TG in the liver of rats with hypothalamic inflammation induced by high fat[D].Doctoral thesis,Chongqing Medical University,Supervisor:Yang G Y,pp.24-38.)
王蕊,安建立,朱悦雨,等.2018.Sfrp5,Klotho血清表达量在糖尿病患者氧化应激、脂肪细胞因子分泌中的相关性[J].临床与病理杂志,38(8):1702-1708.(Wang R,An J L,Zhu Y Y,et al.2018.Relationship of Sfrp5 and Klotho serum levels on oxidative stress and secretion of adipocytokines in diabetic patients[J].Journal of clinical pathology Research,38(08):1702-1708.)
熊本海,罗清尧,赵峰.2016.中国饲料成分及营养价值表(2016年第27版)制订说明[J].中国饲料,(21).(Xiong BH,Luo Q Y,Zhao F.2016.Instructions on the composition and nutritional value of Chinese feed(the 27th edition of 2016)[J].Chinese feed,(21).
左东明,张国栓,何淑兵,等.2018.胃癌组织中Sfrp5的甲基化表达及临床意义[J].河北医药,40(14):2090-2094.(Zuo D M,Zhang G D,He S B,et al.2018.Methylation of sfrp5 in gastric cancer tissues and its clinical significance[J].Hebei Medicine,40(14):2090-2094.)
Anunciado-Koza R P,Higgins D C,Koza R A.2016.Adipose tissue mest and Sfrp5 areconcomitant with variations of adiposity among inbred mouse strains fed anon-obesogenic diet[J].Biochimie,124:134-140.
Bie Z,Sun L,Geng C,et al.2016.MiR-125b regulates SFRP5 expression to promote growth and activation of cardiac fibroblasts[J].Cell Biology International,40(11):1224-1234.
Chang J,Esumi N,Moore K,et al.1999.Cloning and characterization of a secreted frizzled-related protein that is expressed by the retinal pigment epithelium[J].Human Molecular Genetics,8(4):575-583.
Chen H,He Y,Wu D,et al.2016.Bone marrow sfrp5 level is negatively associated with bone formation markers[J].Osteoporosis International,28(4):1-7.
Chen L,Zhao X,Liang G,et al.2017.Recombinant SFRP5protein significantly alleviated intrahepatic inflammation of nonalcoholic steatohepatitis[J].Nutrition&Metabolism,14:56.
Chen Y,Zou D,Wang N,et al.2018.SFRP5 inhibits the migration and invasion of melanoma cells through Wnt signaling pathway[J].OncoTargets and Therapy,11:8761-8772.
Cho Y K,Kang Y M,Lee S E,et al.2018.Effect of SFRP5(Secreted Frizzled-Related Protein 5)on the WNT5A(Wingless-Type Family Member 5A)-Induced endothelial dysfunction and its relevance with arterial stiffness in human subjects[J].Arteriosclerosis,Thrombosis,and Vascular Biology,38(6):1358-1367.
Gahlon H L,Walker A R,Cisneros G A,et al.2018.Reduced structural flexibility for an exonuclease deficient DNApolymeraseⅢmutant[J].Physical Chemistry Chemical Physics,20(42):26892-26902.
Gutiérrez-Vidal R,Vega-Badillo J,Reyes-Fermín L M,et al.2015.SFRP5 hepatic expression is associated with nonalcoholic liver disease in morbidly obese women[J].Annals of Hepatology,14(5):666-674.
Jiadela T,Bin B,Zhou Y W,et al.2018,Association of asymptomatic target organ damage with secreted frizzled related protein 5 in the elderly:The Northern Shanghai Study[J].Clinical Interventions in Aging,13:389-395.
Jura M,Jaros?awska J,Chu D T,et al.2016.Mest and sfrp5are biomarkers for healthy adipose tissue[J].Biochimie,124:124-133.
Li B X,Yao Q,Guo S Q,et al.2018.Type 2 diabetes with hypertensive patients results in changes to features of adipocytokines:Leptin,Irisin,LGR4,and Sfrp5[J].Clinical and Experimental Hypertension,11:1-6.
Li Q,Zuo L L,Lin Y Q,et al.2016.Cloning and expression of SFRP5 in Tibetan Chicken and its relationship with IMF deposition[J].Animal Biotechnology,27(4):231-237.
Lin H W,Fu C F,Chang M C,et al.2018.Similar articles CDH1,DLEC1 and SFRP5 methylation panel as a prognostic marker for advanced epithelial ovarian cancer[J].Epigenomics,10(11):1397-1413.
Liu L B,Chen X D,Zhou X Y,et al.2018.The Wnt antagonist and secreted frizzled-related protein 5:Implications on lipid metabolism,inflammation,and type 2 diabetes mellitus[J].Bioscience Reports,38(4):BSR20180011.
Maekawa T,Kulwattanaporn P,Hosur K,et al.2017.Differential expression and roles of secreted frizzled-related protein 5 and the wingless hmolog Wnt5a in periodontitis[J].Journal of Dental Research,96(5):571-577.
NRC.1994.Nutrition requirement of poultry[S].Washing ton,D.C.National Academy Press.pp.1-3.
Ouchi N,Higuchi A,Ohashi K,et al.2010.Sfrp5 is an anti-inflammatory adipokine that modulates metabolic dysfunction in obesity[J].Science,329(5990):454-457.
van Camp J K,Beckers S,Zegers D,et al.2014.Common genetic variation in sFRP5 is associated with fat distribution in men.Endocrine,46(3):477-484.
van Camp J K,Beckers S,Zegers D,et al.2016.Nucleotide variation of sFRP5 gene is not associated with obesity in children and adolescents[J].Molecular biology reports,43(10):1041-1047.
Wang G,Zhang S,Wei S,et al.2015.Novel polymorphisms of SIX4 gene and their association with body measurement traits in Qinchuan cattle[J].Gene,539(1):107-110.
Yin C,Chu H,Li H,et al.2017.Plasma Sfrp5 and adiponectin levels in relation to blood pressure among obese children[J].Journal of Human Hypertension,31(4):284-291.
Zeng J,Hu J,Lian Y,et al.2018.SFRP5 is a target gene transcriptionally regulated by PPARγin 3T3-L1 adipocytes[J].Gene,641:190-195.
Zhu Z,Yin S,Wu K,et al.2018.Downregulation of Sfrp5 in insulin resistant rats promotes macrophage-mediated pulmonary inflammation through activation of Wnt5a/JNK1 signaling[J].Biochemical and Biophysical Research Communications,505(2):498-504.
卜小雁,李辉,施晓丽,等.2017.70周龄长顺绿壳蛋鸡HO-1基因在不同组织中表达差异研究[J].中国畜牧兽医,44(06):1762-1768.(Bu X Y,Li H,Shi X L,et al.2017.Study on different expression of HO-1 gene of 70-weeks-old changshun blue-shelled chicken in different tissues[J].China Animal Husbandry&Veterinary Medicine,44(6):1762-1768.)
崔焕先.2011.肉鸡肌内脂肪形成的分子调控网络及相关基因研究[D].博士学位论文,中国农业科学院,导师:文杰.pp.1-5.(Cui H Y.2011.The research on molecular regulatory network and gene related intramuscular fat deposition in chicken[D].Thesis for Ph.D.,Chinese Academy of Agricultural Sciences,Supervisor:Wen J.pp.1-5.)
付守艺,闫峰宾,陈毅,等.2018.鸡miR-15a对胸肌肌内脂肪沉积的调控作用[J].畜牧与兽医,50(4):60-65.(Fu S Y,Yan F B,Chen Y,et al.2018.The function of miR-15a in regulating intramuscular fat deposition in pectoralis muscle in chicken[J].Animal Husbandry&Veterinary Medicine,50(4):60-65.)
李晓存,户国,王守志,等.2011.鸡脂肪性状重要候选基因的连锁不平衡模式分析[J].农业生物技术学报,19(2):278-286.(Li X C,Hu G,Wang S Z,et al.2011.Linkage disequilibrium patterns of polymorphisms in candidate genes for fatness in chickens[J].Journal of Agricultural Biotechnology,19(2):278-286.)
刘榜.2007.家畜育种学[M].北京:中国农业出版社,pp.241-248.(Liu B.2007.Animal Breeding[M].Beijin:China Agricultural Press,pp.241-248.)
吕慈.2012.脂肪因子Sfrp5与肥胖及其相关代谢紊乱的相关性研究[D].硕士学位论文,第三军医大学,导师:陈兵.pp.9-26.(Lv C.2012.The relationship of adipokine SFRP5 with obesity and obesity-linked metabolic disorders[D].Master's degree thesis,Third Military Medical University,Supervisor:Chen B,pp.9-26.)
农业部种猪质量监督检验测试中心,2004,NY/T 821-2004,猪肌肉品质测定技术规范[S],北京:中国标准出版社出版,pp.2-6.(Ministry of agriculture breeding pig quality supervision,inspection and testing center,2005,Technical specification for determination of pig muscle quality[M],Beijing:Published by China standard press,pp.2-6).
田茗源.2016.中枢输注Sfrp5对高脂诱导下丘脑炎症大鼠肝脏VLDL-TG的分泌调控及其机制[D].博士学位论文,重庆医科大学,导师:杨刚毅.pp.24-38.(Tian MY.2016.Secretion regulation and mechanism of central infusion of Sfrp5 on VLDL-TG in the liver of rats with hypothalamic inflammation induced by high fat[D].Doctoral thesis,Chongqing Medical University,Supervisor:Yang G Y,pp.24-38.)
王蕊,安建立,朱悦雨,等.2018.Sfrp5,Klotho血清表达量在糖尿病患者氧化应激、脂肪细胞因子分泌中的相关性[J].临床与病理杂志,38(8):1702-1708.(Wang R,An J L,Zhu Y Y,et al.2018.Relationship of Sfrp5 and Klotho serum levels on oxidative stress and secretion of adipocytokines in diabetic patients[J].Journal of clinical pathology Research,38(08):1702-1708.)
熊本海,罗清尧,赵峰.2016.中国饲料成分及营养价值表(2016年第27版)制订说明[J].中国饲料,(21).(Xiong BH,Luo Q Y,Zhao F.2016.Instructions on the composition and nutritional value of Chinese feed(the 27th edition of 2016)[J].Chinese feed,(21).
左东明,张国栓,何淑兵,等.2018.胃癌组织中Sfrp5的甲基化表达及临床意义[J].河北医药,40(14):2090-2094.(Zuo D M,Zhang G D,He S B,et al.2018.Methylation of sfrp5 in gastric cancer tissues and its clinical significance[J].Hebei Medicine,40(14):2090-2094.)
Anunciado-Koza R P,Higgins D C,Koza R A.2016.Adipose tissue mest and Sfrp5 areconcomitant with variations of adiposity among inbred mouse strains fed anon-obesogenic diet[J].Biochimie,124:134-140.
Bie Z,Sun L,Geng C,et al.2016.MiR-125b regulates SFRP5 expression to promote growth and activation of cardiac fibroblasts[J].Cell Biology International,40(11):1224-1234.
Chang J,Esumi N,Moore K,et al.1999.Cloning and characterization of a secreted frizzled-related protein that is expressed by the retinal pigment epithelium[J].Human Molecular Genetics,8(4):575-583.
Chen H,He Y,Wu D,et al.2016.Bone marrow sfrp5 level is negatively associated with bone formation markers[J].Osteoporosis International,28(4):1-7.
Chen L,Zhao X,Liang G,et al.2017.Recombinant SFRP5protein significantly alleviated intrahepatic inflammation of nonalcoholic steatohepatitis[J].Nutrition&Metabolism,14:56.
Chen Y,Zou D,Wang N,et al.2018.SFRP5 inhibits the migration and invasion of melanoma cells through Wnt signaling pathway[J].OncoTargets and Therapy,11:8761-8772.
Cho Y K,Kang Y M,Lee S E,et al.2018.Effect of SFRP5(Secreted Frizzled-Related Protein 5)on the WNT5A(Wingless-Type Family Member 5A)-Induced endothelial dysfunction and its relevance with arterial stiffness in human subjects[J].Arteriosclerosis,Thrombosis,and Vascular Biology,38(6):1358-1367.
Gahlon H L,Walker A R,Cisneros G A,et al.2018.Reduced structural flexibility for an exonuclease deficient DNApolymeraseⅢmutant[J].Physical Chemistry Chemical Physics,20(42):26892-26902.
Gutiérrez-Vidal R,Vega-Badillo J,Reyes-Fermín L M,et al.2015.SFRP5 hepatic expression is associated with nonalcoholic liver disease in morbidly obese women[J].Annals of Hepatology,14(5):666-674.
Jiadela T,Bin B,Zhou Y W,et al.2018,Association of asymptomatic target organ damage with secreted frizzled related protein 5 in the elderly:The Northern Shanghai Study[J].Clinical Interventions in Aging,13:389-395.
Jura M,Jaros?awska J,Chu D T,et al.2016.Mest and sfrp5are biomarkers for healthy adipose tissue[J].Biochimie,124:124-133.
Li B X,Yao Q,Guo S Q,et al.2018.Type 2 diabetes with hypertensive patients results in changes to features of adipocytokines:Leptin,Irisin,LGR4,and Sfrp5[J].Clinical and Experimental Hypertension,11:1-6.
Li Q,Zuo L L,Lin Y Q,et al.2016.Cloning and expression of SFRP5 in Tibetan Chicken and its relationship with IMF deposition[J].Animal Biotechnology,27(4):231-237.
Lin H W,Fu C F,Chang M C,et al.2018.Similar articles CDH1,DLEC1 and SFRP5 methylation panel as a prognostic marker for advanced epithelial ovarian cancer[J].Epigenomics,10(11):1397-1413.
Liu L B,Chen X D,Zhou X Y,et al.2018.The Wnt antagonist and secreted frizzled-related protein 5:Implications on lipid metabolism,inflammation,and type 2 diabetes mellitus[J].Bioscience Reports,38(4):BSR20180011.
Maekawa T,Kulwattanaporn P,Hosur K,et al.2017.Differential expression and roles of secreted frizzled-related protein 5 and the wingless hmolog Wnt5a in periodontitis[J].Journal of Dental Research,96(5):571-577.
NRC.1994.Nutrition requirement of poultry[S].Washing ton,D.C.National Academy Press.pp.1-3.
Ouchi N,Higuchi A,Ohashi K,et al.2010.Sfrp5 is an anti-inflammatory adipokine that modulates metabolic dysfunction in obesity[J].Science,329(5990):454-457.
van Camp J K,Beckers S,Zegers D,et al.2014.Common genetic variation in sFRP5 is associated with fat distribution in men.Endocrine,46(3):477-484.
van Camp J K,Beckers S,Zegers D,et al.2016.Nucleotide variation of sFRP5 gene is not associated with obesity in children and adolescents[J].Molecular biology reports,43(10):1041-1047.
Wang G,Zhang S,Wei S,et al.2015.Novel polymorphisms of SIX4 gene and their association with body measurement traits in Qinchuan cattle[J].Gene,539(1):107-110.
Yin C,Chu H,Li H,et al.2017.Plasma Sfrp5 and adiponectin levels in relation to blood pressure among obese children[J].Journal of Human Hypertension,31(4):284-291.
Zeng J,Hu J,Lian Y,et al.2018.SFRP5 is a target gene transcriptionally regulated by PPARγin 3T3-L1 adipocytes[J].Gene,641:190-195.
Zhu Z,Yin S,Wu K,et al.2018.Downregulation of Sfrp5 in insulin resistant rats promotes macrophage-mediated pulmonary inflammation through activation of Wnt5a/JNK1 signaling[J].Biochemical and Biophysical Research Communications,505(2):498-504.