摘要
分泌型卷曲相关蛋白5(secreted frizzled related protein 5, SFRP5)通过Wnt信号通路调节脂质代谢,改善胰岛素抵抗,维持炎症平衡,是白色脂肪组织分泌的抗炎症脂肪因子之一。为了探讨SFRP5基因多态性与长顺绿壳蛋鸡(Gallus gallus domesticus)肌肉品质的关系、寻找与蛋鸡肌肉品质的相关分子标记、本研究采用直接测序法检测SFRP5基因外显子的单核苷酸多态,分析其对长顺绿壳蛋鸡胸肌肌肉品质的遗传效应。结果表明:在SFRP5基因外显子3上共检测到2个突变位点,g.23253254 G>A的优势基因型和优势等位基因为GG基因型和G等位基因,频率分别为0.569和0.756;g.23253269 T>G的优势基因型和优势等位基因为TT基因型和T等位基因,频率分别为0.681和0.812,且2个位点的突变并未引起氨基酸的变化,为同义突变。2个多态位点的多态信息含量(PIC)均处于0.25~0.5之间,属于中度多态位点,卡方(χ2)检测显示:2个突变位点的基因型分布均未偏离Hardy-Weinberg平衡。连锁不平衡分析发现,SFRP5基因的2个突变位点间均不存在强连锁不平衡。2个SNPs位点在长顺绿壳蛋鸡群体中发现了3种单倍型H1、H2和H3,其频率分别为0.569、0.187和0.244;6种双倍型H1H1、H1H2、H1H3、H2H2、H2H3和H3H3,频率分别为0.313、0.200、0.313、0.056、0.063和0.056。关联分析表明,SFRP5基因的g.23253254 G>A位点对胸肌粗脂肪含量有显著影响(P<0.05);2个SNPs位点组成的双倍型中,双倍型H3H3(AATT)对胸肌粗脂肪含量的影响达到显著水平(P<0.05),显著高于其他5种双倍型(P<0.05),揭示SFRP5基因的遗传变异能影响长顺绿壳蛋鸡胸肌脂肪沉积,g.23253254 G>A和g.23253269 T>G变异位点可能作为提高长顺绿壳蛋鸡胸肌脂肪的分子标记。本研究为进一步探明SFRP5基因变异调控家禽肌肉品质的分子机制提供了理论依据。
Secreted frizzled-related protein 5(SFRP5) is a crucial anti-inflammatory adipokine secreted from white adipose tissue. It can regulate lipid metabolism, reduce insulin resistance, and keep inflammation balance by Wnt singal pathway. To investigate the relationship between SFRP5 gene polymorphism and muscle quality in Changshun Blue-eggshell chicken(Gallus gallus domesticus), and find molecular markers related to muscle quality. In this study, the single nucleotide polymorphisms(SNPs) of SFRP5 gene were detected by direct sequencing, and its correlation with the meat quality of chest muscle in Changshun Blueeggshell chicken was analyzed. The results showed that 2 SNPs(g.23253254 G>A and g.23253269 T>G) were firstly found in SFRP5 gene exon 3. The dominant genotype and dominant allele of the g.23253254 G>A locus was GG genotype and G allele, and its frequencies were 0.569 and 0.756, respectively. The g.23253269 T>G locus was TT genotype and T allele with frequencies were 0.681 and 0.812, respectively. Two mutation sites of SFRP5 gene were synonymous mutations. Polymorphic information content(PIC) of the 2 loci were between0.25 and 0.5, and belong to moderate polymorphic loci. The chi-square test indicated that the genotypes distribution of 2 SNPs(g. 23253254 G>A and g. 23253269 T>G) were agree with the Hardy-Weinberg equilibrium(P>0.05). There was no strong linkage disequilibrium(LD) between each SNP. Among the flock of Changshun Blue-eggshell chicken, 3 haplotypes and 6 diplotypes were formed on SFRP5 gene. The frequency of 3 haplotypes H1, H2 and H3 were 0.569, 0.187 and 0.244 respectively, and then the frequency of6 diplotypes H1 H1, H1 H2, H1 H3, H2 H2, H2 H3 and H3 H3 were 0.313, 0.200, 0.313, 0.056, 0.063 and 0.056,respectively. Genetic correlation analysis demonstated that g.23253254 G>A locus was significantly correlated with crude fat of chest muscle(P<0.05), and further the diplotype H3 H3 was obviously higher crude fat content than other 5 diplotypes(P<0.05). The present research revealed that SFRP5 gene had significant genotype effects on chicken meat quality and also suggested that both g.23253254 G>A and g.23253269 T>G variant sites of SFRP5 gene may be used as molecular markers to improve meat quality in Changshun Blueeggshell chicken. This study provide theoretical basis for further exploring the molecular mechanism of SFRP5 gene variation in poultry muscle quality.
引文
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