2015-2018年河北省廊坊市食源性沙门氏菌分子分型及耐药监测的研究
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摘要
目的掌握廊坊市食源性沙门氏菌的分子分型及耐药状况,为沙门氏菌引起的食源性疾病的暴发预警、溯源及抗生素的合理应用提供科学依据。方法对2015-2018年廊坊市食品中分离出的24株沙门氏菌进行脉冲场凝胶电泳(PFGE),并利用BIOFSUN系列微生物药敏检测系统对菌株进行药敏检测。结果 24株沙门氏菌PFGE分型带型分布广泛,血清型与PFGE条带表现为聚类一致性。24株沙门氏菌耐药现象严重,均存在不同程度的耐药。14株菌对2种以上抗生素耐药,耐药率高达70. 83%。其中伦敦沙门氏菌、肠炎沙门氏菌、鼠伤寒沙门氏菌尤为严重。结论廊坊市各区、县食品存在着较高的食品安全风险问题。某些区县的食品污染高度怀疑源于同一污染源,存在沙门氏菌污染暴发的风险;且沙门氏菌呈现不同程度的抗生素耐药现象,某些菌株存在多重耐药。
Objective To understand the molecular classification and drug resistance of food-borne Salmonella in Langfang City,and provide scientific evidence for the outbreak warning,traceability and reasonable application of antibiotics caused by Salmonella. Methods The pulsed-field gel electrophoresis( PFGE) of 24 strains of Salmonella isolated from Langfang food in2015-2018 was carried out,and the strains were tested for drug susceptibility by BIOFSUN series of microbial susceptibility testing system. Results The PFGE patterns of 24 strains of Salmonella were widely distributed,and the serotypes and PFGE bands showed clustering consistency. 24 strains of Salmonella were severely resistant,and there were different degrees of drug resistance. 14 strains were resistant to more than 2 kinds of antibiotics,and the drug resistance rate was as high as 70. 83%. Among them,Salmonella London,Salmonella enteritidis,and Salmonella typhimurium are particularly serious. Conclusion Foods in all districts and counties in Langfang City have high food safety risks. After PFGE cluster analysis,food contamination in some districts and counties is highly suspected to originate from the same source of pollution,and there is a risk of Salmonella contamination outbreaks; and Salmonella exhibits varying degrees of antibiotic resistance,and some strains are multi-drug resistant.
Objective To understand the molecular classification and drug resistance of food-borne Salmonella in Langfang City,and provide scientific evidence for the outbreak warning,traceability and reasonable application of antibiotics caused by Salmonella. Methods The pulsed-field gel electrophoresis( PFGE) of 24 strains of Salmonella isolated from Langfang food in2015-2018 was carried out,and the strains were tested for drug susceptibility by BIOFSUN series of microbial susceptibility testing system. Results The PFGE patterns of 24 strains of Salmonella were widely distributed,and the serotypes and PFGE bands showed clustering consistency. 24 strains of Salmonella were severely resistant,and there were different degrees of drug resistance. 14 strains were resistant to more than 2 kinds of antibiotics,and the drug resistance rate was as high as 70. 83%. Among them,Salmonella London,Salmonella enteritidis,and Salmonella typhimurium are particularly serious. Conclusion Foods in all districts and counties in Langfang City have high food safety risks. After PFGE cluster analysis,food contamination in some districts and counties is highly suspected to originate from the same source of pollution,and there is a risk of Salmonella contamination outbreaks; and Salmonella exhibits varying degrees of antibiotic resistance,and some strains are multi-drug resistant.
引文
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[11]孙朋浩,邱荣超,李书宁,等.广州市生鲜鸡中肠炎沙门菌的监测及耐药基因的分子鉴定[J].畜牧兽医学报,2018,49(2):388-395.
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[14]梁未丽,王红霞,周海健,等.沙门菌监测点汤卜逊沙门菌分离株的分子分型和耐药性分析[J].疾病监测,2009,24(6):422-425.
[2]李杰,丁承超,翟续昭,等.沙门氏菌检测技术研究进展[J].微生物学杂志,2017,37(4):126-132.
[3]王青青. 2014-2015年杭州经济技术开发区食品安全风险监测结果分析[J].食品安全导刊,2016,10(15):39-40.
[4]贺连华,吴平芳,陈妙玲,等. 2010年-2013年深圳市食品中沙门菌血清分型及耐药分析[J].中国卫生检验杂志,2015,25(2):289-291,294.
[5]孙步武.一起由沙门氏菌Ⅱ引起的食物中毒事件调查[J].应用预防医学,2011,17(1):64.
[6]张晓嫒,王迪,陈倩.北京市食源性非伤寒沙门菌的分子分型和耐药性研究[J].中国食品卫生杂志,2016,27(3):232-236.
[7]王丽,徐建国.脉冲场凝胶电泳技术(PFGE)在分子分型中的应用现状[J].疾病监测,2006,21(5):276-279.
[8]王岚,贾华云,张红,等.湖南省食源性沙门氏菌血清型分布及耐药性研究[J].实用预防医学,2011,18(6):994-997.
[9]吴蜀豫.食品中沙门氏菌污染的主动监测及其耐药基因播散新机制的研究[D].成都:四川大学,2004.
[10]张晓嫒,王迪,陈倩,北京市食源性非伤寒沙门菌的分子分型和耐药性研究[J].中国食品卫生杂志,2016,27(3):232-236.
[11]孙朋浩,邱荣超,李书宁,等.广州市生鲜鸡中肠炎沙门菌的监测及耐药基因的分子鉴定[J].畜牧兽医学报,2018,49(2):388-395.
[12]李楠,赵思俊,王娟,等.肉鸡生产链中肠炎沙门氏菌耐药性分析及ERIC-PCR分型[J].食品科学,2016,37(3):120-124.
[13]郭云昌,刘秀梅.市售鸡肉中沙门菌分离株多重耐药谱测定[J].中国食品卫生杂志,2005,17(2):100-103.
[14]梁未丽,王红霞,周海健,等.沙门菌监测点汤卜逊沙门菌分离株的分子分型和耐药性分析[J].疾病监测,2009,24(6):422-425.