复方苦参水杨酸(足光散)的含量检测
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摘要
通过高效液相色谱(HPCL)测量足光散的几种有效成分及含量,拟找到一种方法可以检测出足光散的主要成分的含量。采用Agilent 1260型高效液相色谱仪,C18柱,流动相:甲醇-0.1 mol/L磷酸二氢钠溶液(93∶7和80∶20),用磷酸调节pH值至4;流速:1.5 mL/min;柱温:35℃;检测波长:240 nm;进样量:10μL。结果表明该方法能够简便高效地测出足光粉的几种有效成分及含量。
In this study, we measured the contents of several effective components in Zuguangsan by HPLC. Agilent 1260 high performance liquid chromatograph and C18 column were adopted;mobile phase was methanol-0.1 mol/L sodium dihydrogen phosphate solution(93∶7 and 80∶20), pH value adjusted to 4 with phosphoric acid; flow rate was 1.5 mL/min;column temperature was 35℃;detection wavelength was 240 nm and injection amount was 10 μL. The results showed that this method could be used to measure the three effective components in Zuguangsan easily and efficiently.
In this study, we measured the contents of several effective components in Zuguangsan by HPLC. Agilent 1260 high performance liquid chromatograph and C18 column were adopted;mobile phase was methanol-0.1 mol/L sodium dihydrogen phosphate solution(93∶7 and 80∶20), pH value adjusted to 4 with phosphoric acid; flow rate was 1.5 mL/min;column temperature was 35℃;detection wavelength was 240 nm and injection amount was 10 μL. The results showed that this method could be used to measure the three effective components in Zuguangsan easily and efficiently.
引文
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[4]肖琦,阳文武,张钦,等.高效液相色谱法测定足光散中硼酸含量[J].中国药业,2015(18):73-75.
[5]陈长水,朱蓉.高效液相色谱法测定足光散中氧化苦参碱含量[J].中国卫生检验杂志,2016(8):1105-1106.
[6]刘羽.不同产地和不同季节苦参中苦参碱和氧化苦参碱的含量测定[J].山东化工,2016,29(11):72-73.
[2]余小平.高效液相色谱法同时测定足光散中水杨酸、苯甲酸的含量[J].药物分析杂志,2005,39(8):955-957.
[3]肖琦,阳文武,李柏群,等.高效液相色谱法测定足光散中苦参碱含量[J].中国药业,2015(17):55-56.
[4]肖琦,阳文武,张钦,等.高效液相色谱法测定足光散中硼酸含量[J].中国药业,2015(18):73-75.
[5]陈长水,朱蓉.高效液相色谱法测定足光散中氧化苦参碱含量[J].中国卫生检验杂志,2016(8):1105-1106.
[6]刘羽.不同产地和不同季节苦参中苦参碱和氧化苦参碱的含量测定[J].山东化工,2016,29(11):72-73.