精子代谢与男性不育的相关性研究进展
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摘要
精子发生和成熟中涉及到多种与代谢相关的过程,任何一种代谢异常都可引起精子活动力、受精能力等受损,最终导致男性不育的发生,如精子中活性氧簇过度产生引起的氧化应激反应,损害了精子的运动和获能;在临床上,男性不育症患者的精浆与正常男性相比,其代谢物成分表现出明显的变化,也可反映精子发育和成熟过程的异常,并间接表明这可能与男性不育相关。本文从精子代谢的相关途径、精子代谢组学检测与精子活动力及临床男性不育症的相关性等方面进行综述。
A variety of metabolism-related courses are involved in spermatogenesis and sperm maturation.Any metabolic abnormality will damage the sperm motility and fertilization, which eventually leads to male infertility. For example, the oxidative stress caused by the excessive production of reactive oxygen in sperm impairs the sperm motility and capacitation. In clinic, the metabolic profiling of seminal plasma in the infertile patients was different from that in the normal male, suggesting the abnormality of sperm development and maturation. This study indirectly showed that there is a potential relevancy between the abnormal metabolism of sperm and male infertility. In this review, we discussed sperm metabolism and the related pathways, sperm metabonomics and tests,the relationship between sperm metabolism and sperm motility, and male infertility.
A variety of metabolism-related courses are involved in spermatogenesis and sperm maturation.Any metabolic abnormality will damage the sperm motility and fertilization, which eventually leads to male infertility. For example, the oxidative stress caused by the excessive production of reactive oxygen in sperm impairs the sperm motility and capacitation. In clinic, the metabolic profiling of seminal plasma in the infertile patients was different from that in the normal male, suggesting the abnormality of sperm development and maturation. This study indirectly showed that there is a potential relevancy between the abnormal metabolism of sperm and male infertility. In this review, we discussed sperm metabolism and the related pathways, sperm metabonomics and tests,the relationship between sperm metabolism and sperm motility, and male infertility.
引文
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[27]Wang Z,Tang WH,Buffa JA,et al.Prognostic value of choline and betaine depends on intestinal microbiota-generated metabolite trimethylamine-N-oxide[J].Eur Heart J,2014,35(14):904-910.
[28]Menni C,Fauman E,Erte I,et al.Biomarkers for type 2 diabetes and impaired fasting glucose using a nontargeted metabolomics approach[J].Diabetes,2013,62(12):4270-4276.
[29]Wang TJ,Ngo D,Psychogios N,et al.2-Aminoadipic acid is a biomarker for diabetes risk[J].J Clin Invest,2013,123(10):4309-4317.
[30]Sharma K,Karl B,Mathew AV,et al.Metabolomics reveals signature of mitochondrial dysfunction in diabetic kidney disease[J].J Am Soc Nephrol,2013,24(11):1901-1912.
[31]Pena MJ,Lambers Heerspink HJ,Hellemons ME,et al.Urine and plasma metabolites predict the development of diabetic nephropathy in individuals with Type 2 diabetes mellitus[J].Diabet Med,2014,31(9):1138-1147.
[32]Zhang X,Diao R,Zhu X,et al.Metabolic characterization of asthenozoospermia using nontargeted seminal plasma metabolomics[J].Clin Chim Acta,2015,450:254-261.
[33]Gilany K,Moazeni-Pourasil RS,Jafarzadeh N,et al.Metabolomics fingerprinting of the human seminal plasma of asthenozoospermic patients[J].Mol Reprod Dev,2014,81(1):84-86.
[34]Zhou X,Wang Y,Yun Y,et al.A potential tool for diagnosis of male infertility:Plasma metabolomics based on GC-MS[J].Talanta,2016,147:82-89.
[35]Qiao S,Wu W,Chen M,et al.Seminal plasma metabolomics approach for the diagnosis of unexplained male infertility[J].PLo S One,2017,12(8):e0181115.
[36]Zhang Z,Zhang Y,Liu C,et al.Serum Metabolomic Profiling Identifies Characterization of Non-Obstructive Azoospermic Men[J].Int J Mol Sci,2017,18(2).pii:E238.
[37]Jafarzadeh N,Mani-Varnosfaderani A,Minai-Tehrani A,et al.Metabolomics fingerprinting of seminal plasma from unexplained infertile men:a need for novel diagnostic biomarkers[J].Mol Reprod Dev,2015,82(3):150.
[38]Kand′ar R,DrabkovaP,MyslíkovaK,et al.Determination of retinol andα-tocopherol in human seminal plasma using an HPLC with UV detection[J].Andrologia,2014,46(5):472-478.
[39]Gilany K,Pouracil RS,Sadeghi MR.Fourier transform infrared spectroscopy:a potential technique for noninvasive detection of spermatogenesis[J].Avicenna J Med Biotechnol,2014,6(1):47-52.
[40]Jayaraman V,Ghosh S,Sengupta A,et al.Identification of biochemical differences between different forms of male infertility by nuclear magnetic resonance(NMR)spectroscopy[J].J Assist Reprod Genet,2014,31(9):1195-1204.
[41]Chen X,Hu C,Dai J,et al.Metabolomics analysis of seminal plasma in infertile males with kidney-yang deficiency:a preliminary study[J].Evid Based Complement Alternat Med,2015,2015:892930.
[2]Mukai C,Travis AJ.What sperm can teach us about energy production[J].Reprod Domest Anim,2012,47(Suppl 4):164-169.
[3]Liu J,Sun CM,Zhang CL,et al.Location and characterization of GAPDS in male reproduction[J].Urol Int,2013,90(4):449-454.
[4]Tourmente M,Villar-Moya P,Rial E,et al.Differences in ATP Generation Via Glycolysis and Oxidative Phosphorylation and Relationships with Sperm Motility in Mouse Species[J].J Biol Chem,2015,290(33):20613-20626.
[5]Nakamura N,Dai Q,Williams J,et al.Disruption of a spermatogenic cell-specific mouse enolase 4(eno4)gene causes sperm structural defects and male infertility[J].Biol Reprod,2013,88(4):90.
[6]Odet F,Gabel SA,Williams J,et al.Lactate dehydrogenase C and energy metabolism in mouse sperm[J].Biol Reprod,2011,85(3):556-564.
[7]Huang Z,Danshina PV,Mohr K,et al.Sperm function,protein phosphorylation,and metabolism differ in mice lacking successive sperm-specific glycolytic enzymes[J].Biol Reprod,2017,97(4):586-597.
[8]Paoli D,Pelloni M,Gallo M,et al.Sperm glyceraldehyde 3-phosphate dehydrogenase gene expression in asthenozoospermic spermatozoa[J].Asian J Androl,2017,19(4):409-413.
[9]Takei GL,Miyashiro D,Mukai C,et al.Glycolysis plays an important role in energy transfer from the base to the distal end of the flagellum in mouse sperm[J].J Exp Biol,2014,217(Pt 11):1876-1886.
[10]Aitken RJ,De Iuliis GN,Finnie JM,et al.Analysis of the relationships between oxidative stress,DNA damage and sperm vitality in a patient population:development of diagnostic criteria[J].Hum Reprod,2010,25(10):2415-2426.
[11]TvrdaE,Kovacˇik A,TusˇimovaE,et al.Antioxidant efficiency of lycopene on oxidative stress-induced damage in bovine spermatozoa[J].J Anim Sci Biotechnol,2016,7(1):50.
[12]Ko EY,Sabanegh ES Jr,Agarwal A.Male infertility testing:reactive oxygen species and antioxidant capacity[J].Fertil Steril,2014,102(6):1518-1527.
[13]La Maestra S,De Flora S,Micale RT.Effect of cigarette smoke on DNA damage,oxidative stress,and morphological alterations in mouse testis and spermatozoa[J].Int J Hyg Environ Health,2015,218(1):117-122.
[14]Lavranos G,Balla M,Tzortzopoulou A,et al.Investigating ROS sources in male infertility:a common end for numerous pathways[J].Reprod Toxicol,2012,34(3):298-307.
[15]Sabeti P,Pourmasumi S,Rahiminia T,et al.Etiologies of sperm oxidative stress[J].Int J Reprod Biomed(Yazd),2016,14(4):231-240.
[16]Kovac JR,Pastuszak AW,Lamb DJ.The use of genomics,proteomics,and metabolomics in identifying biomarkers of male infertility[J].Fertil Steril,2013,99(4):998-1007.
[17]Milardi D,Grande G,Vincenzoni F,et al.Proteomics of human seminal plasma:identification of biomarker candidates for fertility and infertility and the evolution of technology[J].Mol Reprod Dev,2013,80(5):350-357.
[18]Aguiar-Pulido V,Huang W,Suarez-Ulloa V,et al.Metagenomics,Metatranscriptomics,and Metabolomics Approaches for Microbiome Analysis[J].Evol Bioinform Online,2016,12(Suppl1):5-16.
[19]Bernini P,Bertini I,Luchinat C,et al.Individual human phenotypes in metabolic space and time[J].J Proteome Res,2009,8(9):4264-4271.
[20]Ibanez C,SimóC,García-Canas V,et al.Metabolomics,peptidomics and proteomics applications of capillary electrophoresismass spectrometry in Foodomics:a review[J].Anal Chim Acta,2013,802:1-13.
[21]Castro-Puyana M,Herrero M.Metabolomics approaches based on mass spectrometry for food safety,quality and traceability[J].Trends in Analytical Chemistry,2013,52(52):74-87.
[22]Tolstikov V.Metabolomics:Bridging the Gap between Pharmaceutical Development and Population Health[J].Metabolites,2016,6(3).pii:E20.
[23]Patel S,Ahmed S.Emerging field of metabolomics:big promise for cancer biomarker identification and drug discovery[J].J Pharm Biomed Anal,2015,107:63-74.
[24]Joice R,Yasuda K,Shafquat A,et al.Determining microbial products and identifying molecular targets in the human microbiome[J].Cell Metab,2014,20(5):731-741.
[25]Rappaport SM,Barupal DK,Wishart D,et al.The blood exposome and its role in discovering causes of disease[J].Environ Health Perspect,2014,122(8):769-774.
[26]Wishart DS.Emerging applications of metabolomics in drug discovery and precision medicine[J].Nat Rev Drug Discov,2016,15(7):473-484.
[27]Wang Z,Tang WH,Buffa JA,et al.Prognostic value of choline and betaine depends on intestinal microbiota-generated metabolite trimethylamine-N-oxide[J].Eur Heart J,2014,35(14):904-910.
[28]Menni C,Fauman E,Erte I,et al.Biomarkers for type 2 diabetes and impaired fasting glucose using a nontargeted metabolomics approach[J].Diabetes,2013,62(12):4270-4276.
[29]Wang TJ,Ngo D,Psychogios N,et al.2-Aminoadipic acid is a biomarker for diabetes risk[J].J Clin Invest,2013,123(10):4309-4317.
[30]Sharma K,Karl B,Mathew AV,et al.Metabolomics reveals signature of mitochondrial dysfunction in diabetic kidney disease[J].J Am Soc Nephrol,2013,24(11):1901-1912.
[31]Pena MJ,Lambers Heerspink HJ,Hellemons ME,et al.Urine and plasma metabolites predict the development of diabetic nephropathy in individuals with Type 2 diabetes mellitus[J].Diabet Med,2014,31(9):1138-1147.
[32]Zhang X,Diao R,Zhu X,et al.Metabolic characterization of asthenozoospermia using nontargeted seminal plasma metabolomics[J].Clin Chim Acta,2015,450:254-261.
[33]Gilany K,Moazeni-Pourasil RS,Jafarzadeh N,et al.Metabolomics fingerprinting of the human seminal plasma of asthenozoospermic patients[J].Mol Reprod Dev,2014,81(1):84-86.
[34]Zhou X,Wang Y,Yun Y,et al.A potential tool for diagnosis of male infertility:Plasma metabolomics based on GC-MS[J].Talanta,2016,147:82-89.
[35]Qiao S,Wu W,Chen M,et al.Seminal plasma metabolomics approach for the diagnosis of unexplained male infertility[J].PLo S One,2017,12(8):e0181115.
[36]Zhang Z,Zhang Y,Liu C,et al.Serum Metabolomic Profiling Identifies Characterization of Non-Obstructive Azoospermic Men[J].Int J Mol Sci,2017,18(2).pii:E238.
[37]Jafarzadeh N,Mani-Varnosfaderani A,Minai-Tehrani A,et al.Metabolomics fingerprinting of seminal plasma from unexplained infertile men:a need for novel diagnostic biomarkers[J].Mol Reprod Dev,2015,82(3):150.
[38]Kand′ar R,DrabkovaP,MyslíkovaK,et al.Determination of retinol andα-tocopherol in human seminal plasma using an HPLC with UV detection[J].Andrologia,2014,46(5):472-478.
[39]Gilany K,Pouracil RS,Sadeghi MR.Fourier transform infrared spectroscopy:a potential technique for noninvasive detection of spermatogenesis[J].Avicenna J Med Biotechnol,2014,6(1):47-52.
[40]Jayaraman V,Ghosh S,Sengupta A,et al.Identification of biochemical differences between different forms of male infertility by nuclear magnetic resonance(NMR)spectroscopy[J].J Assist Reprod Genet,2014,31(9):1195-1204.
[41]Chen X,Hu C,Dai J,et al.Metabolomics analysis of seminal plasma in infertile males with kidney-yang deficiency:a preliminary study[J].Evid Based Complement Alternat Med,2015,2015:892930.