中国小麦周8425B/中国春RIL群体成株抗叶锈基因的QTL定位
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摘要
为确定小麦叶锈病抗病基因的QTL检测及定位,找到能与抗病基因紧密连锁的分子标记,以小麦品种周8425B,中国春及其杂交获得的244个F_(2∶8) RIL群体为试验材料,分别于2014～2015年在河北保定和河南周口进行了田间叶锈病病害严重度调查,获得了群体的表型数据,利用SNP标记和SSR标记进行基因分型,得到了群体基因型数据,运用软件Joinmap和QTL Ici Mapping 3.1进行连锁作图和QTL定位。结果找到2个QTL位点,分别位于2B,7D染色体上。
In order to determine QTL detection and mapping of wheat leaf rust resistance gene and find the molecular markers that can be closely linked to the resistance gene, the wheat variety Zhou 8425B, Chinese Spring, and 244 F_(2∶8) RIL populations obtained from their hybridization were used as the experimental materials. The disease severity of leaf rust was investigated in Baoding, Hebei province and Zhoukou, Henan province from 2014 to 2015 respectively, and phenotypic data of the population were obtained. SNP markers and SSR markers were used for genotyping to obtain population genotype data, and software Joinmap and QTL Ici Mapping 3.1 were used for linkage mapping and QTL mapping. As a result, 2 QTL loci were found on chromosomes 2B and 7D respectively. These QTL and their closely linked molecular markers can be used for durable disease resistance breeding of wheat in China.
In order to determine QTL detection and mapping of wheat leaf rust resistance gene and find the molecular markers that can be closely linked to the resistance gene, the wheat variety Zhou 8425B, Chinese Spring, and 244 F_(2∶8) RIL populations obtained from their hybridization were used as the experimental materials. The disease severity of leaf rust was investigated in Baoding, Hebei province and Zhoukou, Henan province from 2014 to 2015 respectively, and phenotypic data of the population were obtained. SNP markers and SSR markers were used for genotyping to obtain population genotype data, and software Joinmap and QTL Ici Mapping 3.1 were used for linkage mapping and QTL mapping. As a result, 2 QTL loci were found on chromosomes 2B and 7D respectively. These QTL and their closely linked molecular markers can be used for durable disease resistance breeding of wheat in China.
引文
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[19] Bansal U K,Hayden M J,Venkata B P,et al.Genetic mapping of adult plant leaf rust resistance genes Lr48 and Lr49 in common wheat[J].Theoretical and Applied Genetics,2008,117(3):307-312.
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[21] Herrera-Foessel S A,Singh R P,Huerta-Espino J,et al.Lr68:a new gene conferring slow rusting resistance to leaf rust in wheat[J].Theoretical and Applied Genetics,2012,124(8):1 475-1 486.
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[24] 李星,杨文香,李亚宁,等.小麦抗叶锈基因Lr19的SSR标记[J].中国农业科学,2005,38(6):1 156-1 159.
[25] Suenaga K,Singh R P,Huerta-Espino J,et al.Tagging of slow rusting genes for leaf rust,Lr34 and Lr46,using microsatellite markers in wheat[J].JIRCAS Research Highlights,2001,93:881-890.
[26] Blaszczyk L,Goyeau H,Huang X Q,et al.Identifying leaf rust resistance genes and mapping gene Lr37 on the microsatellite map of wheat[J].Cellular and Molecular Biology Letters,2004,9(48):869-878.
[27] Raupp W J,Sukhwinder-Singh G L,Brown-Guedira B S,et al.Cytogenetic and molecular mapping of the rust resistance gene Lr39 in wheat[J].Theoretical and Applied Genetics,2001,102(2-3):347-352.
[28] 张娜,杨文香,李亚宁,等.小麦抗叶锈基因Lr45的SSR分子标记[J].作物学报,2007,33(4):657-662.
[29] McIntosh R A,Dubcovsky J,Rogers W J,et al.Catalogue of gene symbols for wheat:2010 supplement[J].Annu Wheat Newslett,2010,56:273-283.
[30] Hiebert C,Thomas J,McCallum B.Locating the broad-spectrum wheat leaf rust resistance gene Lr52 (LrW) to chromosome 5B by a new cytogenetic method[J].Theoretical and Applied Genetics,2005,110(8):1 453-1 457.
[31] Michelmore R W,Paran I,Kesseli R V.Identification of markers linked to disease-resistance genes by bulked segregantanalysis:A rapid method to detect markers in specific genomic regions by using segregating populations[J].Proc Natl Acad Sci U S A,1991,88(21):9 828-9 832.
[32] 李德炎.小麦育种学[M].北京:科学出版社,1976:93.
[33] 董玉琛,郑殿升.中国小麦遗传资源[M].北京:中国农业出版社,2000:224.
[34] Sharp P J,Kreis M,Shewry P R,et al.Location of β-amylase sequence in wheat and its relatives[J].Theoretical and Applied Genetics,1988,75(2):286-290.
[35] Gao F M,Wen W E,Liu J D,et al.Genome-Wide Linkage Mapping of QTL for Yield Components,Plant Height and Yield-Related Physiological Traits in the Chinese Wheat Cross Zhou 8425B/Chinese Spring[J].Frontiers in Plant Science,2015,6:1 099.
[36] Li Zaifeng,Lan Caixia,He Zhonghu,et al.Overview and application of QTL for adult plant resistance to leaf rust and powdery mildew in wheat[J].Crop Sci,2014,54(5):1 907-1 925.
[37] Wang Cuifen,Yin Guihong,Xia Xianchun,et al.Molecular mapping of a new temperature-sensitive gene LrZH22 for leaf rust resistance in Chinese wheat cultivar Zhoumai 22[J].Molecular Breeding,2016,36(2):18.
[38] Piech J,Supryn S.Location of adult-plant leaf rust resistance on chromosome 7D in Chinese Spring wheat[J].Cereal Res Commun,1978,6:367-375.
[2] 金夏红,冯国华,刘东涛,等.小麦抗叶锈病遗传研究进展[J].麦类作物学报,2017,37(4):504-512.
[3] 王竹林,刘曙东,王辉,等.小麦慢病性的遗传育种研究进展[J].麦类作物学报,2006,26(1):129-134.
[4] Krattinger S G,Lagudah E S,Spielmeyer W,et al.A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat[J].Science,2009,323(5 919):1 360-1 363.
[5] Dyck P L.Genetics of leaf rust resistance in three introductions of common wheat[J].Can J Genet Cytol,1977(19):711-716.
[6] Dyck P L.The association of a gene for leaf rust resistance with the chromosome 7D suppressor of stem rust resistance in common wheat[J].Genome,1987(29):467-469.
[7] Singh R P.Genetic association of leaf rust resistance gene Lr34 with adult plant resistance to stripe rust in bread wheat[J].Phytopathology,1992,82(8):835-838.
[8] Singh R P.Genetic association of gene Bydvl for tolerance to barley yellow dwarf virus with genes Lr34 and Yr18 for adult plant resistance to rusts in bread wheat[J].Plant Disease,1993,77(11):1 103-1 106.
[9] Spielmeyer W,McIntosh R A,Kolmer J,et al.Powdery mildew resistance and Lr34/Yr18 genes for durable resistance at a locus on the short arm of chromosome 7D of wheat[J].Theoretical and Applied Genetics,2005,111(4):731-735.
[10] McIntosh R A,Devos K M,Dubcovsky J,et al.Catalogue of gene symbols for wheat:2001 Supplement[J].Wheat Information Service,2001(93):40-60.
[11] McIntosh R A,Devos K M,Dubcovsky J,et al.Catalogue of gene symbols for wheat:2002 Supplement[J].Wheat Information Service,2002 (95):50-80.
[12] Yuan J H,Liu T G,Chen W Q.Postulation of leaf rust resistance genes in 47 new wheat cultivarsat seedling stage[J].Scientia Agricultura Sinica,2007,40:1 925-1 935.
[13] Singh S,Bowden R L.Molecular mapping of adult-plant race-specific leaf rust resistance gene Lr12 in bread wheat[J].Molecular Breeding.2011,28(2):137-142.
[14] Kolmer J A.Enhanced leaf rust resistance in wheat conditioned by resistance gene pairs with Lr13[J].Euphytica,1992,61(2):123-130.
[15] Hiebert C W,Thomas J B,Somers D J,et al.Microsatellite mapping of adult-plant leaf rust resistance gene Lr22a in wheat[J].Theoretical and Applied Genetics,2007,115(6):877-884.
[16] Park R F,McIntosh R A.Adult plant resistances to Puccinia recondita f.sp.tritici in wheat[J].New Zealand Journal of Crop and Horticultural Science,1994,22(2):151-158.
[17] William H M,Hoisington D,Singh R P,et al.Detection of quantitative trait loci associated with leaf rust resistance in bread wheat[J].Genome,1997,40:253-260.
[18] William M,Singh R P,Huerta-Espino J,et al.Genetics and resistance molecular marker mapping of leaf rust resistance gene Lr46 and its association with stripe rust resistance gene Yr29 in wheat[J].Phytopathol,2003,93(2):153-159.
[19] Bansal U K,Hayden M J,Venkata B P,et al.Genetic mapping of adult plant leaf rust resistance genes Lr48 and Lr49 in common wheat[J].Theoretical and Applied Genetics,2008,117(3):307-312.
[20] Herrera-Foessel S A,Lagudah E S,Huerta-Epino J,et al.New slow-rusting leaf rust and stripe rust resistance genes Lr67 and Yr46 are pleiotropic or closely linked[J].Theoretical and Applied Genetics,2011,122(1):239-249.
[21] Herrera-Foessel S A,Singh R P,Huerta-Espino J,et al.Lr68:a new gene conferring slow rusting resistance to leaf rust in wheat[J].Theoretical and Applied Genetics,2012,124(8):1 475-1 486.
[22] Pei-pei ZHANG,Takele Weldu Gebrewahid,Yue ZHOU,et al.Seedling and adult plant resistance to leaf rust in 46 Chinese bread wheat landraces and 39 wheat lines with known Lr genes[J].Journal of Integrative Agriculture,2019,18(5):1 014-1 023.
[23] 贾诗雅,郭惠杰,郭鹏,等.2017年我国小麦叶锈菌生理小种的鉴定[C]//彭友良,王琦.中国植物病理学会2018年学术年会论文集.北京:中国农业科学技术出版社,2018.
[24] 李星,杨文香,李亚宁,等.小麦抗叶锈基因Lr19的SSR标记[J].中国农业科学,2005,38(6):1 156-1 159.
[25] Suenaga K,Singh R P,Huerta-Espino J,et al.Tagging of slow rusting genes for leaf rust,Lr34 and Lr46,using microsatellite markers in wheat[J].JIRCAS Research Highlights,2001,93:881-890.
[26] Blaszczyk L,Goyeau H,Huang X Q,et al.Identifying leaf rust resistance genes and mapping gene Lr37 on the microsatellite map of wheat[J].Cellular and Molecular Biology Letters,2004,9(48):869-878.
[27] Raupp W J,Sukhwinder-Singh G L,Brown-Guedira B S,et al.Cytogenetic and molecular mapping of the rust resistance gene Lr39 in wheat[J].Theoretical and Applied Genetics,2001,102(2-3):347-352.
[28] 张娜,杨文香,李亚宁,等.小麦抗叶锈基因Lr45的SSR分子标记[J].作物学报,2007,33(4):657-662.
[29] McIntosh R A,Dubcovsky J,Rogers W J,et al.Catalogue of gene symbols for wheat:2010 supplement[J].Annu Wheat Newslett,2010,56:273-283.
[30] Hiebert C,Thomas J,McCallum B.Locating the broad-spectrum wheat leaf rust resistance gene Lr52 (LrW) to chromosome 5B by a new cytogenetic method[J].Theoretical and Applied Genetics,2005,110(8):1 453-1 457.
[31] Michelmore R W,Paran I,Kesseli R V.Identification of markers linked to disease-resistance genes by bulked segregantanalysis:A rapid method to detect markers in specific genomic regions by using segregating populations[J].Proc Natl Acad Sci U S A,1991,88(21):9 828-9 832.
[32] 李德炎.小麦育种学[M].北京:科学出版社,1976:93.
[33] 董玉琛,郑殿升.中国小麦遗传资源[M].北京:中国农业出版社,2000:224.
[34] Sharp P J,Kreis M,Shewry P R,et al.Location of β-amylase sequence in wheat and its relatives[J].Theoretical and Applied Genetics,1988,75(2):286-290.
[35] Gao F M,Wen W E,Liu J D,et al.Genome-Wide Linkage Mapping of QTL for Yield Components,Plant Height and Yield-Related Physiological Traits in the Chinese Wheat Cross Zhou 8425B/Chinese Spring[J].Frontiers in Plant Science,2015,6:1 099.
[36] Li Zaifeng,Lan Caixia,He Zhonghu,et al.Overview and application of QTL for adult plant resistance to leaf rust and powdery mildew in wheat[J].Crop Sci,2014,54(5):1 907-1 925.
[37] Wang Cuifen,Yin Guihong,Xia Xianchun,et al.Molecular mapping of a new temperature-sensitive gene LrZH22 for leaf rust resistance in Chinese wheat cultivar Zhoumai 22[J].Molecular Breeding,2016,36(2):18.
[38] Piech J,Supryn S.Location of adult-plant leaf rust resistance on chromosome 7D in Chinese Spring wheat[J].Cereal Res Commun,1978,6:367-375.